Journal of Cellular Biotechnology最新文献

{"title":"Hydrogen Sulfide in the Cardiovascular System: A Small Molecule with Promising Therapeutic Potential","authors":"I. Tikhomirova, A. Muravyov","doi":"10.20944/PREPRINTS202101.0193.V1","DOIUrl":"https://doi.org/10.20944/PREPRINTS202101.0193.V1","url":null,"abstract":"this review summarizes current knowledge of the hydrogen sulfide role in cardiovascular system, the proposed mechanisms of its action and the prospects for its applicability in the treatment of cardiovascular diseases. Hydrogen sulfide was recently recognized as gasotransmitter – simple signaling molecule which freely penetrates the cell membrane and regulates a number of biological functions. In humans endogenous H2S is generated via enzymatic and non-enzymatic pathways and its content varies in different tissues and is strictly regulated. In cardiovascular system H2S is produced by myocardial, vascular and blood cells and regulates a number of vital functions. Numerous experimental data prove that endogenously generated as well as exogenously administered H2S exerts a wide range of actions in cardiovascular system, including vasodilator/vasoconstrictor effects, regulation of blood pressure, pro-apoptotic and anti-proliferative effects in the vascular smooth muscle cells, influence on angiogenesis and erythropoiesis, myocardial cytoprotection in ischemia-reperfusion injury, oxygen sensing, inhibition of platelet aggregation and blood coagulation, modification of erythrocyte microrheological properties (aggregability and deformability). Understanding of molecular mechanisms of H2S action and molecular crosstalk between H2S, NO, and CO is essential for the development of its diagnostic and therapeutic potential.","PeriodicalId":15286,"journal":{"name":"Journal of Cellular Biotechnology","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-01-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45376951","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comparative efficiency of three gasotransmitters (nitric oxide, hydrogen sulfide and carbon monoxide): Analysis on the model of red blood cell microrheological responses","authors":"A. V. Muravyov, I. Tikhomirova, P. Avdonin, S. Bulaeva, J. Malisheva","doi":"10.3233/jcb-200023","DOIUrl":"https://doi.org/10.3233/jcb-200023","url":null,"abstract":"BACKGROUND: It is now known regulatory effect of gaseous mediators in many bodily functions. These mediators include nitric oxide (NO), carbon monoxide (CO) and hydrogen sulfide (H2S). However, detailed data on the regulatory role of each of these gasotransmitters (GTs) are still not sufficiently studied. OBJECTIVE: The aim of this study was to investigate on models of microrheological behaviour of intact red blood cells and their recovered ghosts of cellular responses to all three known gasotransmitters: NO, H2S, and CO. METHODS: In experiments with intact red blood cells (RBCs) and their recovered ghosts (filled with an isotonic solution of known viscosity), deformability (RBCD) and aggregation (RBCA) were recorded after incubation of cells with GT donors or stimulators of their endogenous synthesis. RESULTS: It was found that all three GT donors moderately increased the deformability of both intact RBCs and their recovered ghosts (by 5–10%, p < 0.05). In addition GT donors and substrates of their endogenous synthesis significantly reduced RBCA, from 20 to 37% (p < 0.01). Experiments with inhibition of soluble guanylate cyclase (s-GC) almost completely eliminated the RBCD increasing effect of GT donors CONCLUSION: In present study it was demonstrated that all three known gaseous mediators: NO, CO and H2S cause moderate statistically significant positive alterations in RBC deformability as well as a noticeable decrease in RBC aggregation. This was confirmed both in experiments with gasotransmitter donors and stimulators of GT endogenous synthesis. In addition, the data obtained suggest that RBC microrheological responses to the actions of all three GTs are associated with activation of the guanylate cyclase signalling cascade.","PeriodicalId":15286,"journal":{"name":"Journal of Cellular Biotechnology","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.3233/jcb-200023","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"70024795","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Tissue engineering in periodontics- A demystifying review","authors":"Shivani Sachdeva, H. Saluja, Amit Mani, M. Phadnaik, U. Palekar","doi":"10.3233/jcb-200028","DOIUrl":"https://doi.org/10.3233/jcb-200028","url":null,"abstract":"INTRODUCTION: Novel concept known as tissue engineering is for the betterment of human. The use of much advanced molecular science and cell biology in processing the tissues to regenerate even after the loss of inborn tendency of pluripotent cells to multiply is possible by this new therapy. CONTENT: Periodontal tissue regeneration in both height and function is attributed to a complete recovery of the periodontal structures, that is, the formation of alveolar bone, a new connective attachment through collagen fibers as well as functionally oriented on the newly formed cementum is regeneration. Cell based therapies including tissue regeneration is an alternative approach for the regeneration of tissues damaged by disease or trauma. SUMMARY: Though tissue engineering requires the fundamentals of all the three keys namely genomics, proteomics and biometrics to give the solutions to biological problems appearing in dentistry as well as medical sciences.","PeriodicalId":15286,"journal":{"name":"Journal of Cellular Biotechnology","volume":"68 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.3233/jcb-200028","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"70025438","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Practical evaluation of two commercial immunoassays for the quantification of steroid hormones in human saliva","authors":"Jonas Schmidt, F. Wenzel, F. Blessing","doi":"10.3233/jcb-209012","DOIUrl":"https://doi.org/10.3233/jcb-209012","url":null,"abstract":"BACKGROUND: There are certain issues using saliva as a sample for steroid hormone analysis in clinical diagnostics. Therefore, it is important to test whether currently available assays are suitable for this approach in a routine laboratory. OBJECTIVES: The analytical performance of two commercially available immunoassays was compared under routine conditions. In addition, salivary steroid hormone levels were compared with serum levels. METHODS: Saliva and serum samples were collected from healthy subjects. Afterwards, the concentration of cortisol, testosterone, progesterone and estradiol in saliva was measured by using the ADIVA Centaur® XP platform and commercially available manual ELISA kits. Serum levels of the selected steroid hormones were determined by using the ADVIA Centaur® platform. RESULTS: Currently, the ADVIA Centaur® XP platform cannot be used for the quantification of steroid hormones in saliva due to an insufficient calibration range. Commercially available ELISA kits on the other side are well suitable for this application. CONCLUSIONS: There are commercially available immunoassays which are sensitive enough for the determination of steroid hormones from saliva and can be used under routine conditions. However, the interpretation of salivary steroid hormone levels stays a challenging task due to the large variability of the results.","PeriodicalId":15286,"journal":{"name":"Journal of Cellular Biotechnology","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.3233/jcb-209012","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"70026140","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Immunomodulatory response and serum level of neutrophil gelatinase associated lipocalin (NGAL) as a marker of acute kidney injury in wistar rats exposed to pyrethroids","authors":"A. Atere, Olumide Faith Ajani, Akinpelu Moronkeji, Victory Oluwaseun Ajibade, H. Osadolor","doi":"10.3233/jcb-210038","DOIUrl":"https://doi.org/10.3233/jcb-210038","url":null,"abstract":"BACKGROUND: Insecticide usage has increased in the tropics and subtropics due to the high prevalence of vector-borne infections, even though insecticide use effectively reduces insect-borne diseases. Insecticide exposure can cause oxidative stress and have severe consequences for human health. The study was then designed to evaluate oxidative stress and its effects on immunomodulatory and renal integrity among Wistar rats exposed to pyrethroids. METHODS: Eighty-four Wistar rats were randomly selected and divided into two groups. Fifty-one rats were exposed to 1.2 %w/v pyrethroids, while the remaining thirty-three rats were grouped as non-exposed. The groups were divided into three different groups, each with 7, 21, and 41 days. After days of exposure, the animals in each group were anesthetized, and blood samples were collected from the inferior vena cava. Using standard spectrophotometric techniques, the levels of total antioxidant status (TAS), malondialdehyde (MDA), glutathione (GSH), hydrogen peroxide (H2O2), urea, creatinine and uric acid were determined. Blood activities of superoxide dismutase (SOD), glutathione peroxidase (GPX), and catalase (CAT) were determined. ELISA was used to determine levels of IgG, IgA, IgE, TNF-α, and NGAL. Data obtained were statistically compared. RESULTS: The serum mean levels of SOD, GPx, CAT, GSH, and TAS were significantly reduced (p <  0.05) while mean levels of MDA, H2O2, IgG, IgE, IgA, TNFα, neutrophil gelatinase-associated lipocalin (NGAL), urea, uric acid, and creatinine were significantly elevated (p <  0.05) from 7 to 41 days exposure in exposed groups. NGAL had a higher area under the ROC curve than urea and creatinine. CONCLUSIONS: This study observed that pyrethroids can cause oxidative stress, deplete antioxidant levels, nephrotoxicity, and may modulate both humoral and cellular immune functions. It also established NGAL as a sensitive diagnostic tool and early biomarker for acute kidney injury (AKI).","PeriodicalId":15286,"journal":{"name":"Journal of Cellular Biotechnology","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.3233/jcb-210038","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"70026516","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Convalescent plasma therapy: A promising solution for SARS-CoV-2 outbreak","authors":"A. Singh, A. Chaterjee, S. Sirohi, Nikhil Sharma, A. Kathuria","doi":"10.3233/jcb-200024","DOIUrl":"https://doi.org/10.3233/jcb-200024","url":null,"abstract":"Severe Acute Respiratory Syndrome Coronavirus-2 (SARS-CoV-2) has caused more than 18 million confirmed cases and 700000 deaths worldwide after the outbreak in November 2019 (COVID-19). It has been considered as most pathogenic infection under this category. Till date none of the therapeutics or prophylaxis measures have been claimed by any researcher which could cure the patient suffering from the SARS-CoV-2 infection. There is an urgent need for any alternative and effective way of disease management for COVID-19. However, convalescent plasma therapy (CPT) has gain attention of researchers with significant improvement of patient survival rate. Interestingly, there are numerous examples where CPT has proven its potential upon post-exposure prophylaxis and/or treatment in various diseases including COVID-19. This review summarizes the essential elements related to CPT, its past clinical evidences and application of CPT for the management of SARS-CoV-2 infection during COVID-19. We used the published literatures from PubMed, EMBASE and Medline databases until 31st May 2020.","PeriodicalId":15286,"journal":{"name":"Journal of Cellular Biotechnology","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2020-12-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.3233/jcb-200024","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42404369","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Whole blood based cytokine release assay assesses cellular immunity in hepatitis E resolver patients","authors":"W. Dammermann, K. Bröker, F. Bentzien, K. Börner, Katharina Heinzel, S. Lüth","doi":"10.3233/JCB-189007","DOIUrl":"https://doi.org/10.3233/JCB-189007","url":null,"abstract":"","PeriodicalId":15286,"journal":{"name":"Journal of Cellular Biotechnology","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-01-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.3233/JCB-189007","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43547707","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Immersion technique for the preparation of protein gradients on Collagen Type I membranes to control cell migration","authors":"S. Berger, Jan Kiebist, Elisabeth Pötschke, K. Salchert","doi":"10.3233/JCB-180012","DOIUrl":"https://doi.org/10.3233/JCB-180012","url":null,"abstract":"","PeriodicalId":15286,"journal":{"name":"Journal of Cellular Biotechnology","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-01-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.3233/JCB-180012","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45515530","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Tyramide signal amplification as universal detection method on protein coated microbeads","authors":"Christoph Jurischka, F. Dinter, M. Sowa, J. Noack, Juliane Schiebel, D. Roggenbuck, P. Schierack, S. Rödiger","doi":"10.3233/JCB-189003","DOIUrl":"https://doi.org/10.3233/JCB-189003","url":null,"abstract":"","PeriodicalId":15286,"journal":{"name":"Journal of Cellular Biotechnology","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-01-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.3233/JCB-189003","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47257216","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Photoacids in biochemical applications","authors":"H. Kagel, M. Frohme, J. Glökler","doi":"10.3233/JCB-189004","DOIUrl":"https://doi.org/10.3233/JCB-189004","url":null,"abstract":"BACKGROUND: After excitation with light photoacids can change the pH in a solution by release of a proton. They have been used mostly for excited state proton transfer studies. In this review the general functionality and mechanisms and the subdivision of photoacids is explained. STATE OF THE ART: Different uses of photoacids are described, covering a wide range of various biochemical topics, focusing on biochemical applications. Examples for the introduced subdivisions are covered. CONCLUSIONS AND OUTLOOK: The areas in which photoacids can be employed are diverse. Photoacids have a promising future in biotechnology and biochemistry and should be considered for upcoming applications, especially in non-invasive control of biochemical reactions.","PeriodicalId":15286,"journal":{"name":"Journal of Cellular Biotechnology","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-01-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.3233/JCB-189004","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42545205","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}