{"title":"Biofilm-Mediated Heavy Metals Bioremediation in PGPR Pseudomonas","authors":"A. Meliani, A. Bensoltane","doi":"10.4172/2155-6199.1000370","DOIUrl":"https://doi.org/10.4172/2155-6199.1000370","url":null,"abstract":"It is often reported that biofilm-grown cells exhibit enhanced tolerance toward adverse environmental stress conditions, and thus there has been a growing interest in recent years to use biofilms for biotechnological applications such as the uptake of heavy metal. We present in this study the promising application of Pseudomonas biofilms in heavy metal uptake. The main objective of this study is to investigate if these isolates can withstand metal toxicity, and concomitantly to evaluate the interaction between heavy metals and biofilm formation. Compared to control experiments, all Strains were found to produce a greasy-looking biofilm which varied in thickness from an ‘almost invisible film’ to a paper-thick structure depending on the presence of zinc and lead, they exhibited an important biofilm mass. These findings underline the robustness of biofilms under stress conditions and its potential to maintain a favorable niche in stressful environments with increased heavy metal concentrations. Statistically, the biofilms formation seems to be more correlated to the antibiotics resistance (r=0.73; P<0.05) than the heavy metals resistance (r=0.31; P<0.05). Surprisingly, stationary-phase growing was found to be more resistant than logarithmically growing. There is no direct evidence that links metal resistance in biofilms according to the statistical analysis.","PeriodicalId":15262,"journal":{"name":"Journal of Bioremediation and Biodegradation","volume":"208 1","pages":"1-9"},"PeriodicalIF":0.0,"publicationDate":"2016-09-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"89299990","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Biobutanol Production from Lignocellulosic Biomass: Prospective and Challenges","authors":"Cao Guangli, Yachun Sheng, Liang Zhang, Jinzhu Song, Hua Cong, Junzheng Zhang","doi":"10.4172/2155-6199.1000363","DOIUrl":"https://doi.org/10.4172/2155-6199.1000363","url":null,"abstract":"Biobutanol production from lignocellulosic biomass is considered promising and economically feasible. This paper provides an updated review on development of lignocellulose-based biobutanol production with a focus on the understanding of the structure of the feedstock, pretreatment technologies, and fermentation processes. To enhance butanol production from lignocellulosic materials, strategies in terms of inhibitors detoxification, strains improvement and process integration and optimization are also addressed. Besides, the reviewer attempts to shed light on the challenges and perspectives for the bioconversion of lignocellulosic biomass-to-butanol.","PeriodicalId":15262,"journal":{"name":"Journal of Bioremediation and Biodegradation","volume":"42 1","pages":"1-6"},"PeriodicalIF":0.0,"publicationDate":"2016-08-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"80590395","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Assessments of Heavy Metal Accumulation Capacity of Selected Plant Species for Phytoremediation: A Case Study in Little Akaki River","authors":"Mekonnen Amberber, Fantu Kifle","doi":"10.4172/2155-6199.1000369","DOIUrl":"https://doi.org/10.4172/2155-6199.1000369","url":null,"abstract":"Studies on assessments of heavy metal accumulation capacity of selected plant species were carried out in little Akaki river, Addis Ababa city administration, Ethiopia. The aim was to identify plant species and assess the heavy metal accumulation capacity for the Phytoremediation. The heavy metal concentrations in the leaves of selected plant species grown along little Akaki River, soil and water were determined using Atomic Absorption Spectrophotometer at AAEPA. The water analysis result indicated that there was Cd (0.077 mg/L), Cr (0.90 mg/L), Pb (<0.001 mg/L) and Ni (0.034 mg/L) in the river. Similarly, the soil analysis result indicated that Cr (153, 22 mg/L), Ni (30.71 mg/L) and Pb (9.55 mg/L) were detected from the soil but Cd was not detected. The plant analysis result indicated that the species Arundo donax, Ricinus communis and Vernonia amygdalina had a good potential plant that absorbs Cd, Cr and Pb in different concentrations, while Ni was not detected in the leaves of selected plants. The species Arundo donax accumulated Cr (80.90 mg/L), Pb (37.30 mg/L) and Cd (25.98 mg/L), Vernonia amygdalina accumulated Cr (83.59 mg/L), Cd (44.46 mg/L) and Pb (14.49 mg/L) and Ricinus communis accumulated Cr (62.06 mg/L), Cd (16.64 mg/L) and Pb (16.64 mg/L). It was concluded that the selected plant species had a good accumulation capacity of Cr, Pb and Cd for the phytoremediation activity.","PeriodicalId":15262,"journal":{"name":"Journal of Bioremediation and Biodegradation","volume":"67 1","pages":"1-4"},"PeriodicalIF":0.0,"publicationDate":"2016-08-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"89966322","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
A. P. Schaik, J. Prosser, Doug Graham, J. Xue, L. Booth, J. Horswell
{"title":"The Suitability of Using Vermicomposting for the Stabilization of Septic Tank Waste","authors":"A. P. Schaik, J. Prosser, Doug Graham, J. Xue, L. Booth, J. Horswell","doi":"10.4172/2155-6199.1000368","DOIUrl":"https://doi.org/10.4172/2155-6199.1000368","url":null,"abstract":"An investigation into the viability of vermicomposting septic tank waste was carried out. Progression of the composting process was monitored by analysing a range of chemical and biological parameters (dehydrogenase enzyme activity, Escherichia coli (E. coli), Olsen P, nitrate and ammonium). At the end of the composting period additional parameters were measured such as total C and N, organic matter, pH, soluble P and N, Salmonella spp., Campylobacter spp. and Helminth ova. Dehydrogenase activity and nitrate and ammonia ratios were shown to be useful indicators to determine the maturation of compost. However, reduction in E. coli did not relate well to removal of pathogens such as Campylobacter spp. and Helminth ova. Pasteurising temperatures cannot be achieved during vermiculture as worms are sensitive to thermophile temperatures, thus for wastes containing high levels of pathogens (such as raw sewage or septic tank waste), further treatment may be required to produce a pathogen free compost.","PeriodicalId":15262,"journal":{"name":"Journal of Bioremediation and Biodegradation","volume":"226 1","pages":"1-6"},"PeriodicalIF":0.0,"publicationDate":"2016-08-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"74675192","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Jun Yin, W. Stevenson, Zhiling Guo, B. Koopman, S. Svoronos
{"title":"Effect of Protein Crude Extract on Oxic/Anoxic Diauxic Growth of a Napdeficient Mutant of Paracoccus pantotrophus","authors":"Jun Yin, W. Stevenson, Zhiling Guo, B. Koopman, S. Svoronos","doi":"10.4172/2155-6199.1000367","DOIUrl":"https://doi.org/10.4172/2155-6199.1000367","url":null,"abstract":"In this study it is shown that addition of crude protein extract containing either membrane bound nitrate reductase (Nar) or periplasmic nitrate reductase (Nap) shortens the diauxic lag of denitrifying bacteria switched from aerobic to anoxic conditions. The specific growth rate under anoxic conditions, following resumption of exponential growth, was found to be linearly related to the extract dose.","PeriodicalId":15262,"journal":{"name":"Journal of Bioremediation and Biodegradation","volume":"9 1","pages":"1-5"},"PeriodicalIF":0.0,"publicationDate":"2016-08-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"72661752","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"An Evaluation of Biological Approach for the Effluent Treatment of Paper Boards Industry - An Economic Perspective","authors":"K. Rajkumar","doi":"10.4172/2155-6199.1000366","DOIUrl":"https://doi.org/10.4172/2155-6199.1000366","url":null,"abstract":"The environmental problems of paper board industry are not limited by the high water consumption, generate wastewaters and solid wastes. This paper presents the paper board industry effluent treatment plant components performance and physio-chemical characteristics of wastewater and sludge. In ETP operation most important factors is effluent characterization, flow rate, aeration tank dissolved oxygen (DO) level, mixed liquid suspended solids (MLSS), food to micro-organism ratio (F/M), sludge volume and chemical addition for nutrients and coagulant. The wastewater is characterized by extreme quantities of pH, total dissolved solids (TDS), total suspended solids (TSS), biochemical oxygen demand (BOD), chemical oxygen demand (COD) and the solid waste from ETP sludge is characterized by SEM –EDX and ICP. The wastewater samples were collected from the partied of one month influent and treated effluent. An influent consist pH of 7.1-7.7, TSS of 1160-1380 mg/L, TDS of 1500-2000 mg/L, BOD and COD varies 200-250 mg/L and 2010-1286 mg/L respectively. The treated effluent of pH varies 7.1-7.3, TSS 10-20 mg/L, TDS ranges from 1500-1900 mg/L, BOD and COD ranges from 7-16 mg/L and 54-145 mg/L, respectively. Further solid waste in the form of sludge handled through mechanical dewatering devise (belt press). The result shows that the performance of effluent treatment plant based on the permissible limits prescribed by regulatory agencies and can be recycle an entire treated effluent into the board manufacturing process. The fixed capital cost was 700 lakhs- Indian Rupees (Rs.) and annual operation and maintenance cost was estimated Rs. 180 lakhs (without RO plant). Reuse of treated effluent in pulp process and sludge in sun dry board manufacturing and composting were adopted to achieve the target of sustainable development for ecological and economical gains.","PeriodicalId":15262,"journal":{"name":"Journal of Bioremediation and Biodegradation","volume":"2011 1","pages":"1-13"},"PeriodicalIF":0.0,"publicationDate":"2016-08-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"76749391","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Remediation of Escravous Crude Oil Contaminated Soil Using Activated Carbon from Coconut Shell","authors":"Ibrahim, Rilwan Shuaibu, S. Abdulsalam, S. Giwa","doi":"10.4172/2155-6199.1000365","DOIUrl":"https://doi.org/10.4172/2155-6199.1000365","url":null,"abstract":"Environmental pollution is one of the major hazards facing humanity in the quest for energy today. In Nigeria, the activities of oil exploration and exploitation have repeatedly exposed the environment to the effect of hydrocarbon spill. This research work was carried out to investigate the potential of activated carbon produced from coconut shell to treat Escravous crude oil contaminated soil, by varying pollutant dosage, adsorbent dosage and time. The present study has clearly demonstrated that activated carbon from coconut shell (ACCS) can be used to significantly enhance the rate of degradation of petroleum hydrocarbon in the soil when homogenized with water in a ratio of 20 w/w and studied under an atmospheric temperature. A significant degradation was achieved after 32 days of the remediation process. When the soil to ACCS ratio and crude oil to ACCS were 48 w/w, and 1 mL/g respectively, (AX1) sample code had a ratio of crude to ACCS as 1:1, which reduces the total petroleum hydrocarbon (TPH) from 43.56 to 18.78 residue (mg/L). TPH was also observed to reduce to 2.83 mg/L from initial concentration of 28.92 mg/L when ratio of crude oil to ACCS was 1:1.5 (BX1) sample code, while reduction in THP of 13.63 mL was achieved with 0.5:1.5 ratio of crude oil to ACCS (CX1) sample code. The three (3) results had their rate and percentage of remediation for AX1, BX1, and CX1 as 0.77, 0.82 and 0.43 (mg/L)/day and 56.88%, 90.22% and 92.97% respectively. BX1 approach with the ratio of 1:1.5 has the high rate and percentage remediation more promising compared to AX1 and CX1. Lead which is a big treat to both plants and animals was almost reduced to a Zero (0) percentage.","PeriodicalId":15262,"journal":{"name":"Journal of Bioremediation and Biodegradation","volume":"115 1","pages":"1-6"},"PeriodicalIF":0.0,"publicationDate":"2016-08-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"86208444","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Potential of Salvinia molesta for Removal of Sodium in Textile Wastewater","authors":"M. Pavithra, H. Kousar","doi":"10.4172/2155-6199.1000364","DOIUrl":"https://doi.org/10.4172/2155-6199.1000364","url":null,"abstract":"Textile industries are one of the major contaminators of water and soil. Sodium is the only major cation present in textile wastewater due to high consumption of sodium salts in processing units. Many aquatic macrophytes are successfully used for phytoremediation of contaminated water. An attempt has been made to test the potential of Salvinia molesta in reduction of sodium from textile effluent. The experimental study showed that Salvinia molesta is a suitable candidate for effective removal of sodium from textile wastewater.","PeriodicalId":15262,"journal":{"name":"Journal of Bioremediation and Biodegradation","volume":"217 1","pages":"1-5"},"PeriodicalIF":0.0,"publicationDate":"2016-08-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"76486835","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
D. Mutua, E. Njagi, G. Orinda, Geoffry Obondi, F. Kansiime, J. Kyambadde, J. Omara, R. Odong, Hellen Butungi
{"title":"Biological Treatment of Meat Processing Wastewater Using Lab-Scale Anaerobic-Aerobic/Anoxic Sequencing Batch Reactors Operated in Series","authors":"D. Mutua, E. Njagi, G. Orinda, Geoffry Obondi, F. Kansiime, J. Kyambadde, J. Omara, R. Odong, Hellen Butungi","doi":"10.4172/2155-6199.1000362","DOIUrl":"https://doi.org/10.4172/2155-6199.1000362","url":null,"abstract":"In the eastern Africa sub-region, many industries discharge untreated effluents to nearby water resources, thereby polluting the environment. This is because the technologies applicable for wastewater treatment are expensive for these small-medium sized companies with low profit margins. Slaughterhouses belong to this category of industrial setup. The objective of this study was to investigate treatment of meat processing wastewater using anaerobic– aerobic/anoxic Sequencing Batch Reactors (SBRs) operated in series. Reactors were operated for one year using meat processing wastewater. Hydraulic retention time was 2 days for the anaerobic SBR, and 1 day for the aerobic/ anoxic SBR while the organic loading was 12.8 kg COD/m3/day. In the anaerobic SBR, removal efficiencies for total and soluble chemical oxygen demand (TCOD and SCOD), total suspended solids (TSS) and turbidity were 79, 76, 79, and 70%, respectively, with effluent mean concentrations of 3554 ± 58 mg/L, 762 ± 3 mg/L, 2307 ± 21, and 2800 ± 9 FAU. Conductivity, ammonia-nitrogen, ortho-phosphates and total phosphorus concentrations increased by 38, 80, 81 and 71%. Pollutant removal efficiencies in the aerobic/anoxic SBR were 98, 96, 97, 89, 74, 97, 91, 90, and 86% for TCOD, SCOD, BOD, TSS, turbidity, ammonium nitrogen (NH4 +–N), total nitrogen (TN), orthophosphorus (o-PO4 3-–P), and total phosphorus (TP), respectively. Except TKN (35 ± 4 mg/L) and o-PO4 3-–P (8 ± 1 mg/L), all other parameters in the aerobically treated effluent met national discharge standards. Thus, abattoir effluent can be treated using anaerobic–aerobic/anoxic SBR system.","PeriodicalId":15262,"journal":{"name":"Journal of Bioremediation and Biodegradation","volume":"25 1","pages":"1-6"},"PeriodicalIF":0.0,"publicationDate":"2016-07-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"75287353","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"In Vitro Biodegradation of Palm Oil Mill Effluent (POME) by Bacillus subtilis, Pseudomonas aeruginosa and Aspergillus niger","authors":"O. Loretta, E. Stephen, A. Ezeata, E. Usman","doi":"10.4172/2155-6199.1000361","DOIUrl":"https://doi.org/10.4172/2155-6199.1000361","url":null,"abstract":"In vitro Comparison of Palm Oil Mill Effluent (POME) degradation was carried out using Bacillus subtilis, Pseudomonas aeruginosa and Aspergillus niger over a period of 16 days using POME as carbon source. pH, nitrate, phosphate, turbidity, and bacterial counts were carried out at intervals of 0, 4, 8, 12, and 16 days to assess the progress of degradation. There was appreciable increase in pH and turbidity in medium containing the organisms while phosphate and nitrate decreased with time. Colony counts showed a decline after day 8 in all organisms. The counts in P. aeruginosa and B. subtilis were higher than that of A. niger throughout the period of study. There were no significant differences (p>0.05) in the pH and nitrate while significant differences were observed in the turbidity, phosphate and colony counts at 5% probability level. Gas Chromatography Mass Spectrophotometric (GCMS) analysis carried out on days 0, 7, and 14 for Pseudomonas aeruginosa and Aspergillus niger showed decrease in peaks after day 7 while samples containing B. subtilis showed no reduction in peak numbers rather new compounds were formed in the process. At the end of the study, it was deduced that P. aeruginosa was able to efficiently utilize POME as a carbon source for growth better than Aspergillus niger and Bacillus subtilis and could play a great role in large scale treatment of palm oil effluent.","PeriodicalId":15262,"journal":{"name":"Journal of Bioremediation and Biodegradation","volume":"28 1","pages":"1-8"},"PeriodicalIF":0.0,"publicationDate":"2016-07-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"74941384","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}