{"title":"Degradation of Profenofos and λ-Cyhalothrin Using Endogenous Bacterial Isolates and Detection of the Responsible Genes","authors":"R. Abdullah, Sherif B Abde, Ghani, N. Sukar","doi":"10.4172/2155-6199.1000360","DOIUrl":"https://doi.org/10.4172/2155-6199.1000360","url":null,"abstract":"An effective profenofos and λ-cyhalothrin degrading bacterium (strain DB17) was isolated from soil samples collected from Qassim region, Saudi Arabia. Based on the results of phylogenetic similarity of 16SrDNA gene sequences, strain DB17 was identified to be Pseudomonas putida. The isolate utilized profenofos and λ-cyhalothrin as the sole source of carbon for its growth. Analytical method was developed and validated for the determination of profenofos and λ-cyhalothrin in bacterial medium to monitor the intended biodegradation of both compounds. The inoculation of isolate DB17 109 and 1011 cells/ml to mineral salt medium supplemented by 100 mg/liter of profenofos and λ-cyhalothrin resulted in a higher degradation rate than in non-inoculated medium. The genes encoding organophosphorus hydrolase (mpd and opd) and pyrethroid-degrading esterase gene (pytY) were cloned using a PCR cloning strategy. The obtained results highlight the potential of this bacterium to be used in the cleanup of pesticide contaminated sites.","PeriodicalId":15262,"journal":{"name":"Journal of Bioremediation and Biodegradation","volume":"18 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2016-07-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"80800569","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Microalgal-Bacterial Consortium in Polyaromatic Hydrocarbon Degradation of Petroleum Based Effluent","authors":"Egberomoh Godsgift Omojevwe, F. Ezekiel","doi":"10.4172/2155-6199.1000359","DOIUrl":"https://doi.org/10.4172/2155-6199.1000359","url":null,"abstract":"The recurrent and indiscriminate discharge of flow stations' crude oil effluent has led to an increased polyaromatic hydrocarbons (PAHs) level in the environment. PAHs are high-priority environmental pollutants of significant human health concerns, thus considerable effort has been made in this study to biodegrade these PAHs using microalgalbacterial consortia. Crude oil effluent samples collected from the saver pit at Egbaoma flow station in the Niger Delta region of Nigeria were analysed biochemically and microbiologically. Three species of bacteria and two species of microalgae isolated, characterized and screened emerged as best hydrocarbon utilizers. The consortia comprised of Chlorella minutissimma and Aphanocapsa sp. as microalgae inoculants, while Citrobacter sp. SB9, Pseudomonas aeruginosa SA3 and Bacillus subtilis SA7 as bacterial inoculants. Growth dynamics, pH and degradation of the effluents' PAH content were analyzed for a period of ten days. Consortium BCC (bacteria inoculants and Chlorella minutissimma), BCCA (all inoculants) and BCA (bacteria inoculants and Aphanocapsa sp.) had PAH degradation percentage of 92.09%, 67.76% and 47.19% and optical density (OD) of 0.1255°A, 0.0669°A and 0.0703°A from an initial OD of 0.0126°A respectively. Only BCA had an acidic pH after ten days. Effective synergism and excellent PAH degradation is achievable and highly recommendable with microalgal-bacterial consortium yet its success is highly dependent on the consortium assembling, therefore, proper selection of the microbes for biodegradation is paramount to the success and efficiency of the degradation process.","PeriodicalId":15262,"journal":{"name":"Journal of Bioremediation and Biodegradation","volume":"80 1","pages":"1-5"},"PeriodicalIF":0.0,"publicationDate":"2016-07-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"83950384","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Application of Microbial Culture and Rhamnolipid for Improving the Sedimentation of Oil Sand Tailings","authors":"Soroor Javan Roshtkhari, C. Mulligan","doi":"10.4172/2155-6199.1000358","DOIUrl":"https://doi.org/10.4172/2155-6199.1000358","url":null,"abstract":"Densification of oil sand tailings deposited in the tailing ponds and recovering water from them are two major challenges in the oil sands surface mining industry. A small increase in the tailings settlement rate (which normally is very slow) can improve the densification of tailings and significantly reduce water consumption and the volume of the tailing ponds. In this work, the objective was to evaluate the role of a mixed culture of two microbial strains isolated from weathered oil and rhamnolipid (JBR 425) together with these strains in the sedimentation of fine tailing particles. It has been found that a mixed culture of two microbial strains isolated from weathered oil increased the sedimentation. Rhamnolipid (0.5%) together with these two microbial strains at 15°C ± 2°C showed significant increases in sedimentation (by a factor of 5.1), the concentration of larger particles (by a factor of 2.63), the particle mean diameter (by a factor of 2.70) and flocculation in the tailings samples compared to the control while the zeta potential is still negative. This means that the mechanism of flocculation is probably due to increasing the hydrophobicity of the particles, interaction of biosurfactant and high molecular weight microbial organic compounds through a bridging mechanism with clay particles. This work shows the potential of using rhamnolipid and microbial culture in order to increase the oil sand sedimentation through flocculation and microbial activity in a more environmentally friendly densification process.","PeriodicalId":15262,"journal":{"name":"Journal of Bioremediation and Biodegradation","volume":"60 1","pages":"1-8"},"PeriodicalIF":0.0,"publicationDate":"2016-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"85856952","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Status of Remediation Approaches","authors":"S. Kulshreshtha","doi":"10.4172/2155-6199.1000E176","DOIUrl":"https://doi.org/10.4172/2155-6199.1000E176","url":null,"abstract":"Remediation states for the removal of pollutants from the environment. Remediation can be done in various ways such as by using waste biomass for sorption of pollutants or by living organisms like plants, microbes that cause degradation of pollutants. Bioremediation implies the use of microbes in the removal of heavy metals, industrial wastes and any other type of toxic wastes from the environment. These microbes may be used in viable and non-viable form, natural and modified form. Microbes used in natural form, faced many challenges related to their survival and tolerance to harsh environmental conditions.","PeriodicalId":15262,"journal":{"name":"Journal of Bioremediation and Biodegradation","volume":"31 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2016-06-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"85864373","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Continuing and Emerging Concerns about Contaminants","authors":"B. Ramakrishnan","doi":"10.4172/2155-6199.1000E175","DOIUrl":"https://doi.org/10.4172/2155-6199.1000E175","url":null,"abstract":"","PeriodicalId":15262,"journal":{"name":"Journal of Bioremediation and Biodegradation","volume":"53 1","pages":"1-3"},"PeriodicalIF":0.0,"publicationDate":"2016-06-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"75689751","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Foliar Nutrient Composition of 19 Tree Species Grown on a PhytocappedLandfill Site","authors":"K. Venkatraman, N. Ashwath","doi":"10.4172/2155-6199.1000357","DOIUrl":"https://doi.org/10.4172/2155-6199.1000357","url":null,"abstract":"An alternative landfill capping technique ‘Phytocapping’ (establishment of perennial plants on a layer of soil placed over the waste) was trailed at Rockhampton, Australia, as it is eco-friendly, less expensive and socially acceptable. In this capping, trees are used as ‘Bio-pumps and Screens’ and soil cover as ‘Storage’. They together minimise water percolation leading to reduced leachate production. Twenty one tree species were grown on two depths of soil (700 mm and 1400 mm) and monitored for their growth and their ability to restrict water infiltration through the waste. A very common question raised by most scientist and engineers is growth and survival of tree species in a landfill environment. Hence to determine the conditions and health of the tree species, foliar and foliar litter nutrient concentrations were measured in all the tree species grown on the phytocapped landfill site. The 3 year-old trees showed slightly elevated levels of nutrient and this will continue as the trees mature and develop more roots. The trees in the 700 mm soil cover contained slightly higher leaf concentrations of nutrients due to the possible closer proximity of their roots to the waste.","PeriodicalId":15262,"journal":{"name":"Journal of Bioremediation and Biodegradation","volume":"36 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2016-06-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"77695826","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
P. Prabhavathi, R. Rajendran, S. Karthiksundaram, S. Dineshkumar, P. Santhanam, D. Premnath, G. Ponmari, Manik, A. An, Mi Kyung Kim
{"title":"Molecular Docking Studies on Potent Adsorbed Receptor of Thrh Protein: A New Target for Biodegradation of Indigo Dye","authors":"P. Prabhavathi, R. Rajendran, S. Karthiksundaram, S. Dineshkumar, P. Santhanam, D. Premnath, G. Ponmari, Manik, A. An, Mi Kyung Kim","doi":"10.4172/2155-6199.1000356","DOIUrl":"https://doi.org/10.4172/2155-6199.1000356","url":null,"abstract":"Vat dyes are aromatic compounds widely used for denim textile industries, this result in a great wastewater problem from this industry due to recalcitrant nature of these dyes. The active protein (ThrH) was purified from Pseudomonas aeruginosa by DEAE-Sepharose A-50 column chromatography and this 3D crystal structure was reported recently. The present study aimed to demonstrate the binding energy between 3D crystal structures of indigo dye and ThrH. We have calculated the gliding score as well as gliding energy based on the hydrophobic interactions between targeted sites (amino acid and dye residue) and the main think is binding energy which was observed maximum level because of the presence of magnesium ions along with catalytic molecules located at the binding sites. The dye degraded mineralized compound was predicted by mass spectrum and infrared spectroscopy.","PeriodicalId":15262,"journal":{"name":"Journal of Bioremediation and Biodegradation","volume":"11 1","pages":"1-7"},"PeriodicalIF":0.0,"publicationDate":"2016-06-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"84165955","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Arsenic Occurrence, Ecotoxicity and its Potential Remediation","authors":"Turquoise C Alex, Er, E. Gulledge, F. Han","doi":"10.4172/2155-6199.1000E174","DOIUrl":"https://doi.org/10.4172/2155-6199.1000E174","url":null,"abstract":"Arsenic levels in water, sediments, and biota of the estuaries and coastal ecosystems have been well documented. Natural and anthropogenic activities such as industrial offshore oil spills and groundwater pollution have increased its levels in natural water systems. This brief review provides a clear view on arsenic occurrence, ecotoxicity and its potential remediation. Both humans and biota were affected from arsenic contamination. The development of substitute materials for arsenic applications in the agricultural and forestry industries and controls of arsenic emissions from the coal industry may be possible strategies to significantly decrease arsenic pollution sources and dissipation rates into the environment. At the same, to develop cost effective green remediation technology for cleaning As in water and soils is essential.","PeriodicalId":15262,"journal":{"name":"Journal of Bioremediation and Biodegradation","volume":"9 1","pages":"1-3"},"PeriodicalIF":0.0,"publicationDate":"2016-06-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"74963804","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Xuemei Liu, Samuel Kiwanuka, K. Cleary, D. Ryan, D. Dowling, K. Germaine
{"title":"Use of Ecopiling to Remediate PAH-Contaminated Storm-water LagoonSediment","authors":"Xuemei Liu, Samuel Kiwanuka, K. Cleary, D. Ryan, D. Dowling, K. Germaine","doi":"10.4172/2155-6199.1000355","DOIUrl":"https://doi.org/10.4172/2155-6199.1000355","url":null,"abstract":"Polycyclic Aromatic Hydrocarbons (PAHs) are widely distributed in the environment originating from both natural and anthropogenic sources. Consequently, they are found ubiquitously in the lithosphere, hydrosphere, atmosphere and biosphere. They are of major concern due to their recalcitrant nature, ability to bio-accumulate and their toxic, carcinogenic and mutagenic effects on biological systems. One major anthropogenic source of PAHs is creosote which is used as a wood preservative and contains 85% PAHs (w/w). Industrial facilities carrying out creosote treatment activities can result in major contamination of soil and groundwater at these sites. The current study investigates the potential of Ecopiling as a bioremediation strategy for creosote contaminated soil/sediment at a former wood preservation facility. The study involved greenhouse experiments to assess the phytoremediation potential of perennial ryegrass (Lolium perenne L.) and onsite construction of two small-scale, trial Ecopiles. The greenhouse study showed that the creosote contaminated sediment had an inhibitory effect on plant growth and reduced the effectiveness of rhizoremediation. However, the use of a clean soil overlay led to a significant decrease in the phyto-toxicity experienced by the ryegrass plants and subsequently resulted in greater PAH degradation in these pots. The use of Ecopiling was found to reduce the sum of EPA 16 PAH concentrations in the sediment by 94-96% after 730 days. The incorporation of spent-mushroom compost into the Ecopile, did initially increase the rate of PAH degradation, however, after 730 days there was no significant difference between the PAH concentrations remaining in the two Ecopiles. Although Ecopiling is a slow process compared to thermal and chemical treatments it has proven to be an effective and low cost process for the remediation of PAHs from industrial sediments.","PeriodicalId":15262,"journal":{"name":"Journal of Bioremediation and Biodegradation","volume":"2 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2016-05-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"75639976","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Bioremediation of Soil Contaminated with Tannery Effluent by Combined Treatment with Cow Dung and Microorganisms Isolated from Tannery Effluent","authors":"Akpomie Olubunmi O, Ejechi Bernard O","doi":"10.4172/2155-6199.1000354","DOIUrl":"https://doi.org/10.4172/2155-6199.1000354","url":null,"abstract":"Bioremediation potential of a combination of cow dung and a microbial consortium (Pseudomonas aeruginosa, Penicillium chrysogenum and Aspergillus niger) in soil contaminated with tannery effluent, was investigated in the laboratory. Concentrations of phenol, sulphide and ammonium nitrogen in contaminated soil, were significantly reduced (P=0.000) to permissible levels after treatment with microbial consortium, cow dung or combination of microbial consortium and cow dung. Reduction of these compounds was best with combination of microbial consortium and cow dung. Cr (VI) level (139.2 mg/kg) was significantly reduced (P=0.000-0.050) by 33.2, 96.9 and 99.9% after treatment with microbial consortium, cow dung, and combination of consortium and cow dung, respectively. The treatments elevated soil pH from 5.8 to 6.9-7.2. Growth of maize in soil treated with the combination of microbial consortium and cow dung was not significantly different from growth in uncontaminated soil. Cr (VI) concentrations in the maize tissues were very low (0.002-0.006 mg/g). Thus combination of microbial consortium and cow dung may have potential application in bioremediation of soil polluted by tannery wastes.","PeriodicalId":15262,"journal":{"name":"Journal of Bioremediation and Biodegradation","volume":"19 1","pages":"1-5"},"PeriodicalIF":0.0,"publicationDate":"2016-05-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"80799472","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}