Boletín chileno de parasitología最新文献

{"title":"Inmunodiagnóstico de la triquinosis humana","authors":"M. D. C. Contreras, L. Sandoval, P. Salinas, T. Saavedra, H. Schenone","doi":"10.4067/S0365-94022001000200003","DOIUrl":"https://doi.org/10.4067/S0365-94022001000200003","url":null,"abstract":"An indirect hemagglutination test (IHAT) and an ELISA test for trichinosis using as antigen a larvae soluble fraction from Trichinella spiralis was carried out for the detection of IgG, IgM and IgA specific antibodies in 113 serum samples from patients confirmed or suspected to have trichinosis by strong clinical and epidemiological evidences (Group I). The same tests were also performed on 110 serum samples corresponding to patients without strong evidences of having trichinosis (Group II). In Group I the corresponding sensitivities for RHAI, ELISA IgG, ELISA IgM, ELISA IgA were: 82.3-85.8-88.5 and 88.5% respectively. Seventeen patients were tested again a week after the first analysis (10 of them corresponded to negative ones), increasing the positivity: 23.5-100.0;35.3-100.0;41.2-100.0 and 41.2-100.0% for RHAI, ELISA IgG, ELISA IgM and ELISA IgA, respectively. Other two patients were followed-up for 5 years. IHAT and ELISA IgG remained positive, whereas ELISA IgM and ELISA IgA were constantly negative betweeen 17 and 32 months in one case, and between 48 and 60 months in the other (this last one had presented a severe clinic disease). In the group II, four patients were positive with IHAT, however only one for ELISA IgA, the latter also presented ELISA IgM near the cut off. The use of ELISA IgG, ELSIA IgM and ELISA IgA in the immunodiagnosis of trichinosis is discussed.","PeriodicalId":152182,"journal":{"name":"Boletín chileno de parasitología","volume":"30 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2001-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"115592876","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comportamiento biológico de Triatoma pallidipennis (Hemiptera: Reduviidae) en el estado de Morelos, México","authors":"Norma Bautista, Gloria Rojas, I. Haro, M. Bucio, P. M. Schettino","doi":"10.4067/S0365-94022001000200004","DOIUrl":"https://doi.org/10.4067/S0365-94022001000200004","url":null,"abstract":"T. pallidipennis was found naturally infected in localities of domestic, peridomestic and wild areas in the State of Morelos, Mexico. In agreement with the triatomines high potentiality like vectors of T. cruzi, protozoan that causes Chagas' disease and the knowledge of these bugs in Mexico, the aim of the present research was to collect triatomines for assessment of T. cruzi infection, to characterize and compare life cycles, identification of feeding preferences and observe indexes of experimental infectivity. Seven isolates of T. cruzi were obtained, from differents localities which curves of parasites showed different behaviors. Histopathological study, carried out by counting of amastigotes nests, which affected the gastrocnemius muscle and heard was observed. The life cycles of T. pallidipennis was completed in 131 days. The feeding preferences were rats, cats and pigs.","PeriodicalId":152182,"journal":{"name":"Boletín chileno de parasitología","volume":"43 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2001-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"132659538","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Implementación de un ensayo de ELISA para el diagnóstico de la fascioliasis equina","authors":"L. DaniloVargas, A. SilviaDelPino, I. CarmenGloriaGonzález, O. MacarenaVidal","doi":"10.4067/S0365-94022001000200011","DOIUrl":"https://doi.org/10.4067/S0365-94022001000200011","url":null,"abstract":"And indirect enzyme linked immunosorbent assay was developed for the diagnosis of fascioliasis in naturally infected equines as a screening test with secretory and excretory antigens of adult flukes, in order to support the diagnosis of the disease. Fascioliasis in an economically important disease and the trematode Fasciola hepatica infects a wide variety of mammals and occasionally can infect man. The standarization was performed with negative sera (collected in the XII Region of Chile) and positive sera checked by post mortem examination. The evaluation was done in 226 sera samples: 115 negative, and 70 positive with confirmed fascioliasis, 19 from animals with other pathologies and 22 from apparently healthy animals. The ELISA presented a 85.7% of sensibility and a 97.4% of specificity.","PeriodicalId":152182,"journal":{"name":"Boletín chileno de parasitología","volume":"45 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2001-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"120894700","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Búsqueda de anticuerpos contra Trichinella spiralis en roedores de libre desplazamiento capturados en un zoológico de la Ciudad de México","authors":"J. L. Rosa, A. Gomez-priego","doi":"10.4067/S0365-94022000000300016","DOIUrl":"https://doi.org/10.4067/S0365-94022000000300016","url":null,"abstract":"A serological survey to search for antibodies against T. spiralis was performed in free roaming rats (n=64) and mice (n=35) caught in a zoological park from Mexico City. Serum samples were analyzed by ELISA and immunoelectrotransfer blot assay (EIBT). None serum show positive absorbance values in ELISA nor recognized T. spiralis specific antigenic fractions in EIBT. However, two rat samples recognized three antigens of 31, 37 y 55 kDa, while one of them reacted with two additional antigens of 64 and 67 kDa. As it is known that the antigen epitope profiles varied among Trichinella species, it could be possible that in rats, there is 3% of antibody prevalence against Trichinella sp.; however, due that other organisms could induce the production of cross-reacting antibodies, such conclusion can not be supported at all. These results suggest that T. spiralis was not part of helminthological fauna in these rodents.","PeriodicalId":152182,"journal":{"name":"Boletín chileno de parasitología","volume":"16 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2000-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"121987059","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Descripción de la morfología externa de Duboisiella proloba Baer, 1938 (Trematoda:Strigeida) de Didelphis albiventris en Argentina, por microscopía electrónica de barrido","authors":"A. S. Cruz, O. H. Prieto, J. T. Borda, Nora Scheibler, L. Gómez","doi":"10.4067/S0365-94022000000300014","DOIUrl":"https://doi.org/10.4067/S0365-94022000000300014","url":null,"abstract":"The aim of this study was to report some external morphological features of Duboisiella proloba Baer, 1938. The parasites were collected during necropsy from the small intestine of Didelphis albiventris hunted in its native environment near Corrientes City, Argentina. Collected specimens of Duboisiella proloba were observed by light microscopy in order to be classified. Later the parasites were put into a 3% glutaraldehyde solution, dehydrated, dried by critical point, assembled, metallized, observed and photographed with a SEM. This is the first description of some external morphologic features of this species carried out by SEM in Argentina.","PeriodicalId":152182,"journal":{"name":"Boletín chileno de parasitología","volume":"14 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2000-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"125219647","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Contaminación por helmintos en espacios públicos de la provincia de Chubut, Argentina","authors":"M. G. Zunino, Maria V. De Francesco, Jorge A. Kuruc, Nicolás Schweigmann, M. C. Wisnivesky-Colli, O. Jensen","doi":"10.4067/S0365-94022000000300008","DOIUrl":"https://doi.org/10.4067/S0365-94022000000300008","url":null,"abstract":"Canine-borne helminthiases constitute a worldwide problem. Some of these parasites can originate serious pathologies in humans. Given the potential existence of these zoonoses in Argentina, horizontal surveys on contamination degree evaluated as faeces per hectare and on parasite prevelence in faeces were carried out in six localities of the Province of Chubut. The behaviour of people visiting public places was also analyzed qualitatively. All of the survey sites were contaminated with faeces and with some parasitic forms. Contamination degrees ranged from 32 to 147 faeces/ha. Toxocara sp. (17.4%), Strongyloides sp. (5.1%), and Ancylostoma. sp (5.6%) were the main genera among other parasites detected. As a whole, the analysis revealed that contamintion degree, prevalence and human behaviuor in relation to the exposure to the parasite would be the main factors to be taken into account in formulating control measures. Transmission risk patterns in Chubut were not homogenous; therefore, studies and control strategies should be devised and implemented at the local scale.","PeriodicalId":152182,"journal":{"name":"Boletín chileno de parasitología","volume":"17 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2000-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"127731299","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"La parasitología y la entomología en la narrativa latinoamericana del siglo XX","authors":"H. Schenone","doi":"10.4067/S0365-94022000000300007","DOIUrl":"https://doi.org/10.4067/S0365-94022000000300007","url":null,"abstract":"In the present reviev of twelve pieces produced by distinguished 20th century Latin American writers Jorge Luis Borges from Argentina, Jorge Amado and Joao Ubaldo Ribeiro from Brazil, Jose Donoso from Chile, Gabriel Garcia Marquez from Colombia, Alejo Carpentier from Cuba, Miguel Angel Asturias from Guatemala, Octavio Paz from Mexico, Mario Vargas Llosa from Peru, Horacio Quiroga and Mario Benedetti from Uruguay and Arturo Uslar-Pietri from Venezuela ­ paragraphs or parts of paragraphs in which parasitological or entomological situations of the most varied hues are referred to or described, have been extracted in a selective form. Sometimes in these descriptions appear, local or regional expressions, without ignoring colorful folkloric representations. For a easier interpretation these or part of these paragraph sentences have been arranged by thematic similarities. In a varied and kaleidoscopic vision, it will be possible to find protozooses (malaria, Chagas disease, leishmaniasis, amibiasis), helminthiases (ascariasis, hydatidosis, trichinosis, schistosomiasis, cysticercosis, onchocerciasis), parasitoses produced by arthropods (pediculosis, scabies, tungiasis, myiasis), passing progressivelly to hemaphagous arthropods (mosquitoes, gnats, horse flies, bedbugs, ticks), venomous arthropods (Latrodectus spiders, scorpions, wasps, bees), mechanical vectors (flies and cockroaches), culminating with a conjuction of bucolic arthropods (butterflies, crickets, grasshoppers cicadas, ants, centipedes, beetles, glowworms, dragonflies).","PeriodicalId":152182,"journal":{"name":"Boletín chileno de parasitología","volume":"78 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2000-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"126318419","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Frecuencia de anticuerpos anti Toxoplasma gondii en gatos de la ciudad de Valdivia, Chile","authors":"Francisca Ovalle, Alejandro García, Jorge Thibauth, M. Lorca","doi":"10.4067/S0365-94022000000300012","DOIUrl":"https://doi.org/10.4067/S0365-94022000000300012","url":null,"abstract":"Toxoplasmosis is a parasitic zoonosis. The importance of the cat as related to this parasitosis lies in the fact that it is not only the definite host of the parasite, but responsible for its dissemination through the release of oocysts, which subsequently infect both humans and other animals. The objective of this study was to determine the serological prevalence of Toxoplasma gondii in the feline population of the city of Valdivia (Chile) and to establish the possible epidemiological implications of this prevalence. With these goals in mind the technique of indirect immunofluorescence was implemented to detect anti- T. gondii species--specific IgG. Blood samples from 97 cats (selected using a directed sampling process) from different sectors of Valdivia were collected. The sample included 46 males and 51 females of different ages, Positive and negative control sera obtained from de United States were used to verify the results, observed by UV microscopy. The anti-feline IgG antibody was used as directed by the manufacturer (Sigma). Of the 97 selected cats, 32 were found to be positive with a titre higher than 1:4, a prevalence of 33.0%. The number of infected males as compared to the number of infected females was found to be statistically insignificant, using the chi-square analysis with p less than 0.1. In contrast, a definite correlation between age and seropositivity was found; infection levels were higher in older animals. These results are consistent with those obtained in different studies on this topic that have been performed in different parts of the world. However, they would seem to be in conflict with other, similar studies that have been done recently in Chile; this may be due to the fact that the cats selected for this study were exposed to different climatic conditions than those examined in previous works. In conclusion, it has been determined that in Valdivia exist cats infected by T. gondii, which indicate the presence of the necessary epidemiological conditions for the persistence of this parasitic cycle and the source of infection for humans and other animals.","PeriodicalId":152182,"journal":{"name":"Boletín chileno de parasitología","volume":"283 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2000-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"127719599","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Frecuencia de infección por Diphyllobothrium sp. (Cestoda: Diphyllobothriidae) en carnívoros silvestres de Argentina","authors":"F. A. Martínez, J. C. Troiano, L. G. Añasco, A. Duchene, A. N. J. Siscardi","doi":"10.4067/S0365-94022000000300013","DOIUrl":"https://doi.org/10.4067/S0365-94022000000300013","url":null,"abstract":"With the aims to determine the infection frequency by tapeworms of Diphyllobothrium genus 30 samples from captive wild carnivoves were analyzed. A 30% of the animal analyzed was positive to the infection. Whereas the family Procionidae has a high percentage of positivity (60%), Canidae have lower infection ratio (20-25%). The accuracy for the diagnosis of Diphylobothrium was made by the morphology of scolex, proglottids and eggs. This is the first report of the parasite presence in Argentine wild carnivores.","PeriodicalId":152182,"journal":{"name":"Boletín chileno de parasitología","volume":"142 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2000-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"114405141","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Relación hospedador - parásito entre tres cepas de Schistosoma mansoni y seis cepas de Biomphalaria glabrata de áreas endémicas y no endémicas venezolanas","authors":"Liboria Matinella, L. Pino, G. Morales","doi":"10.4067/S0365-94022000000300005","DOIUrl":"https://doi.org/10.4067/S0365-94022000000300005","url":null,"abstract":"Six lots of 18 B. glabrata from: La Victoria, Turmero, Cagua in Aragua state; Caserio El 25 in Carabobo state, Chabasquen in Portuguesa state and Humocaro Bajo in Lara state, were experimentally infected with miracidia of SM, C5 and C6 strains of Schistosoma mansoni (18 snails/Schistosoma mansoni strain). The averages of the intramolluscal period (IMP) obtained for the S. mansoni strains were very similar and comprised between 35.4 and 36.1 days. No significative statistical differences in the IMP were found according to the S. mansoni strain and the size of snails: < 7 mm and 7 mm. However, significative statistical differences in the IMP were found, in relation to the B. glabrata strain and between the snails classifiel in two groups according to the S. mansoni dose (5 miracidia/snail and 10 miracidia/snail). The higher percentages of infection (PI) were found for the following parasite-snail combinations: C6-Cas. El 25 (80.7%), SM-La Victoria (73.1%) and C5-Cagua (62%). No significative statistical differences were found for the PI a) between the snail classifiel in two groups according to the size (<7 mm and 7 mm), b) in relation to the miracidium dosification (5 and 10 miracidia/ snail and c) in accord to the S. mansoni strain. However, significative statistical differences were found for the PI obtained with different strains of the snail.","PeriodicalId":152182,"journal":{"name":"Boletín chileno de parasitología","volume":"26 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2000-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"121480783","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}