Journal of Berry Research最新文献

{"title":"From the chokeberry fruit products and by-products to health-promoting effects through multifaceted in vitro bioactivity evaluation and molecular docking studies","authors":"Milica Milutinović, Nada Ćujić Nikolić, Milica Radan, Tatjana Mihajilov Krstev, Katarina Šavikin, Predrag Petrović, Milica Randjelović, Bojana Miladinović, Suzana Branković, Dušanka Kitić","doi":"10.3233/jbr-230066","DOIUrl":"https://doi.org/10.3233/jbr-230066","url":null,"abstract":"BACKGROUND:\u0000Polyphenolic-rich chokeberry extracts and juice could provide health benefits in humans. Moreover, by-products after processing stand out as a potential new source of valuable compounds. OBJECTIVE:\u0000The research aimed to evaluate in vitro bioactivity of chokeberry fruit extract, juice, and waste extract (material remaining after juice processing) based on their chemical composition and molecular docking analysis. METHODS:\u0000Spectrophotometric methods were used to determine the phenolics, anthocyanins, flavonoids, and proanthocyanins content in freeze-dried samples. Additionally, individual anthocyanins, flavonoids, and sugars were identified through HPLC analysis. The antioxidant capacity was tested using two in vitro methods and assessing antimicrobial activity. The effects of tyrosinase and acetylcholinesterase inhibition were examined using spectrophotometric methods. Molecular docking analysis identified the interaction of chokeberry components with the active sites of these enzymes. RESULTS:\u0000The waste extract exhibited the most robust antioxidant activity within the DPPH system. While bactericidal activity was absent across all chokeberry preparations, they demonstrated inhibitory effects on specific microbiological strains relevant to the gastrointestinal tract. Results revealed the chokeberry products’ ability to inhibit tyrosinase and acetylcholinesterase. Molecular docking confirmed the strong interactions between chokeberry compounds and these enzymes. CONCLUSION:\u0000The results indicate the potential of chokeberry products and by-products as valuable resources for pharmaceutical applications.","PeriodicalId":15194,"journal":{"name":"Journal of Berry Research","volume":"126 1","pages":""},"PeriodicalIF":1.7,"publicationDate":"2024-04-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140805456","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Pre-harvest treatments: A different insight into preservation of strawberries","authors":"Jingcheng Xu, Ying Wang, Lin Chai, Danping Yin, Tingwei Lin, Yujia Tao, Shudong Liu, Huijuan Qi, Xianyi Gao, Jingyong Jiang","doi":"10.3233/jbr-240001","DOIUrl":"https://doi.org/10.3233/jbr-240001","url":null,"abstract":"Strawberry is one of the most favored consumed fresh fruits worldwide. However, the major constraint limiting future sustainable production and sales of strawberries is post-harvest decay. Thus, taking a deep look into the effective preservation measures including pre- and post-harvest techniques to retain freshness and inhibit the pathological spoilage of strawberries in the supply chain is of great significance and necessity. At present, relying solely on post-harvest technology is not enough to fix the aim of strawberry fruit preservation and longer shelf life. On the contrary, pre-harvest treatments could be extend shelf life of strawberry fruit and adapt to the diverse post-harvest environments. This review give the latest pre-harvest treatments, their effects on post-harvest quality and storability of strawberries, and develop the expectation of strawberries pre-harvest methods applicated in the future.","PeriodicalId":15194,"journal":{"name":"Journal of Berry Research","volume":"43 1","pages":""},"PeriodicalIF":1.7,"publicationDate":"2024-04-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140571282","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effects of 60Co γ-irradiation treatment on the diversity of fungal microflora in blueberries during cold storage","authors":"Feng Wang, Chengdu Miao, Peiming Ye, Lukai Ma, Qin Wang, Dongjie Liu, Gengsheng Xiao","doi":"10.3233/jbr-230038","DOIUrl":"https://doi.org/10.3233/jbr-230038","url":null,"abstract":"In the present study, the effects of 60Co-γ irradiation on the diversity of fungal microflora in blueberries during cold storage have been explored in detail. The effects of irradiation on the fungal microflora in blueberries could be observed at the lowest effective dose of 1.0 kGy. The genome library of fungal microflora in blueberries was evaluated by tusing Shannon, Simpson, Chao1, and ACE indices, presenting good coverage of blueberry samples. Principal coordinate analysis clarified the differences in the evolution and clustering of fungi in blueberries under different irradiation levels. The composition and diversity of fungal microflora in blueberries treated with different doses of radiation exhibited significant differences under cold storage of 4°C. However, most of the fungi belonged to the Ascomycota at the phylum. The fungal microflora was relatively stable at the initial stage of cold storage, however, the diversity of fungal species in irradiated blueberries varied greatly in the middle stage around the 15th day. This comprehensive study provides deeper insight about the effects of irradiation on the evolution of fungal microflora in blueberries during cold storage. The findings of this study lay a good foundation for post-harvest storage and preservation of blueberries.","PeriodicalId":15194,"journal":{"name":"Journal of Berry Research","volume":"62 1","pages":""},"PeriodicalIF":1.7,"publicationDate":"2024-03-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140075030","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The preventive and inhibitory effects of red raspberries on cancer","authors":"Zexiu Qi, Bei Yang, Francesca Giampieri, Danila Cianciosi, José Miguel Alvarez-Suarez, Maria Elexpuru-Zabaleta, Josè L. Quiles, Tamara Y. Forbes-Hernandez, Di Zhang, Weibin Bai, Lingmin Tian, Bruno Mezzetti, Maurizio Battino, Yasmany Armas Diaz","doi":"10.3233/jbr-240004","DOIUrl":"https://doi.org/10.3233/jbr-240004","url":null,"abstract":"Red raspberries are gaining attention more and more for their nutritional and bioactive components, with potential health effects such as antitumor properties. This review aims to describe the antioxidant activities of red raspberries, emphasizing the role of anthocyanins and ellagitannins as primary contributors among red raspberry polyphenols; it also outlined the connection between red raspberries and their role in inhibiting cancer cell growth by regulating oxidative stress. Numerous studies suggest that red raspberries are able to block cancer cell progression by inhibiting proliferation, migration, and autophagy, as well as regulating the cell cycle, angiogenesis, and DNA damage repair. This review sheds light to the growing evidence supporting antioxidants as a crucial link between fruit consumption and cancer prevention.","PeriodicalId":15194,"journal":{"name":"Journal of Berry Research","volume":"5 1","pages":""},"PeriodicalIF":1.7,"publicationDate":"2024-03-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140045508","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Investigating the tolerance of different strawberry cultivars to Botrytis cinerea infection and its relation with fruit quality","authors":"Rohullah Qaderi, Luca Mazzoni, Virginia Tonanni, Francesco Gagliardi, Luca Capriotti, Valeria Pergolotti, Yasmany Armas Diaz, Franco Capocasa, Bruno Mezzetti","doi":"10.3233/jbr-230050","DOIUrl":"https://doi.org/10.3233/jbr-230050","url":null,"abstract":"BACKGROUND:\u0000Strawberries are a delicate, high nutritional value fruit with an extremely short shelf life and high susceptibility to tissue infection, mainly by Botrytis cinerea. Control of the disease requires an extensive amount of fungicide that is applied in varying complexes because the pathogen easily develops resistance against the active compounds. Planting tolerant cultivars seems to be a promising alternative for fruit growers, but there are currently no cultivars available combining tolerance to B. cinerea with attractive horticultural traits. OBJECTIVE:\u0000In this study, four well-defined strawberry cultivars (‘Romina’, ‘Cristina’, ‘Silvia’, and ‘Sibilla’) were selected and tested under treatment with Botrytis cinerea to determine the tolerance of each cultivar and its association with fruit quality. METHODS:\u0000Fruits were harvested and then stored for four days after inoculation (treatment) or not (control) with spores of B. cinerea; then, every day the level of decay was detected, and fruits were frozen for successive evaluation of fruit quality. RESULTS:\u0000The ‘Silvia’ cultivar is the one that demonstrated a higher level of tolerance to Botrytis infection during the treatment, and the ‘Romina’ cultivar was the cultivar most susceptible to the treatment. The results of the study also highlighted qualitative changes in all strawberry cultivars infected with Botrytis cinerea, by a decrease in the soluble solids content and an increase of acids. Generally, in all cultivars, phenolic acids, and vitamin C decreased in both control and infected but there was a strong decrease in infected fruit compared to control. Anthocyanin content increased in control fruits but strongly decreased in infected. CONCLUSION:\u0000As a preliminary outcome, a positive correlation was obtained between fruit nutritional quality and tolerance to decay, as a high amount of nutritional compound content provides lower susceptibility of fruits to Botrytis cinerea. To confirm this result more studies need to be done.","PeriodicalId":15194,"journal":{"name":"Journal of Berry Research","volume":"271 1","pages":""},"PeriodicalIF":1.7,"publicationDate":"2024-03-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140045506","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Polyphenols profile and in vitro biological activities of black goji berries ( Lycium ruthenicum Murr.)","authors":"Tijana Ilić, Nemanja Krgović, Dragana D. Božić, Stevan Samardžić, Mirjana Marčetić, Gordana Zdunić, Bojana B. Vidović","doi":"10.3233/jbr-230029","DOIUrl":"https://doi.org/10.3233/jbr-230029","url":null,"abstract":"BACKGROUND:\u0000Due to the presence of wide-range phytochemicals, particularly anthocyanins, and their health-promoting activities, black goji fruits (Lycium ruthenicum Murr., LRf) gained attention as functional foods or nutraceuticals. However, data on the phytochemical composition and bioactivities of LRf from outside Asian countries are very limited. OBJECTIVE:\u0000This study reports the polyphenols profile and in vitro biological activities of LRf cultivated in the southern part of Serbia. METHODS:\u0000The total content of polyphenols, flavonoids and anthocyanins of LRf was determined spectrophotometrically, while the polyphenol profile was evaluated by LC-DAD-ESI-MS. Antioxidant and enzyme-inhibitory potentials against α-amylase, α-glucosidase, acetylcholinesterase, tyrosinase, and 15-lipoxygenase properties were determined spectrophotometrically. The prebiotic potential was assessed by the broth microdilution method. RESULTS:\u0000Chromatographic analysis confirmed that hydroxycinnamic acid derivatives and acylated anthocyanins were the predominant polyphenols of LRf. In addition to antioxidative activities, Lycium ruthenicum methanol extract (LRe) showed inhibitory effects against all tested enzymes (IC50 = 5.28–7.28 mg/mL). Prebiotic potential on the growth of various microorganisms’ species was observed, with a marked effect on the growth of yeast Saccharomyces boulardii. CONCLUSIONS:\u0000The polyphenol pattern of LRf cultivated in Serbia corresponds with the literature data. Observed bioactivities support further phytochemical characterizations of LRf and investigations of their health-promoting effects, especially prebiotic properties.","PeriodicalId":15194,"journal":{"name":"Journal of Berry Research","volume":"16 1","pages":""},"PeriodicalIF":1.7,"publicationDate":"2024-01-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139104739","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Modulatory effect of Andean blackberry polyphenols on genes related to antioxidant and inflammatory responses, the NLRP3 inflammasome, and autophagy","authors":"Beatriz Castejón-Vega, Nikolaos C. Kyriakidis, Elizabet Alcócer-Gómez, Francesca Giampieri, Ana M. González-Paramás, Mario D. Cordero, José M. Alvarez-Suarez","doi":"10.3233/jbr-230058","DOIUrl":"https://doi.org/10.3233/jbr-230058","url":null,"abstract":"BACKGROUND:\u0000The Andean blackberry (Rubus glaucus Benth) is one of Ecuador’s most iconic Andean berries for which a high anthocyanin content has been described. OBJECTIVE:\u0000The aim of the present study was to determine the chemical composition and anti-inflammatory potential of the Andean blackberryfrom Ecuador, with an emphasis on its effects on NLRP3 inflammasome activation and autophagy processes. RESULTS:\u0000Andean blackberry extracts were rich in hydroxycinnamates (coumaric acid and derivates), in addition to quercetin and kaempferol as principal flavonols. Cyanidin and its glycosides were identified as the main anthocyanins present. Andean blackberry extracts efficiently reduced oxidative stress markers in the lipopolysaccharide-stimulated RAW 264.7 cells. The extracts also caused a moderate decrease in the expression of the pro-inflammatory and antioxidant genes NFκB1, TNF, IL-1β, IL-6, and NOS2 expression, while they significantly increased the mRNA levels of both SOD1 and NFE2L2 genes. Andean blackberry extracts significantly decreased the activation of the NLRP3 inflammasome complex, as well as p62 levels, and the LC3I/LC3II ratio increased, suggesting a direct action of Andean blackberry compounds on the inflammatory response and restoration of the autophagy process. CONCLUSIONS:These results suggest that Andean blackberries potentially have an anti-inflammatory effect through their ability to regulate genes related to the inflammatory and antioxidant response, as well as modulate the activation of the NLRP3 inflammasome complex and autophagy processes.","PeriodicalId":15194,"journal":{"name":"Journal of Berry Research","volume":"24 1","pages":""},"PeriodicalIF":1.7,"publicationDate":"2023-12-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139104697","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Assessment of photosynthetic capacity of two blackberry cultivars subjected to salt stress by the JIP fluorescence test","authors":"Ines Mihaljević, Marija Viljevac Vuletić, Vesna Tomaš, Zvonimir Zdunić, Dominik Vuković, Krunoslav Dugalić","doi":"10.3233/jbr-230026","DOIUrl":"https://doi.org/10.3233/jbr-230026","url":null,"abstract":"BACKGROUND:\u0000Salt stress has recently emerged as one of the most significant abiotic stresses with negative impact on crop production. Photosynthesis is a vital biochemical process in plants, very sensitive to environmental constraints, including salt stress. OBJECTIVE:\u0000The aim of this study was toevaluate the impact of salt stress on photosystem II in the leaves of blackberry cultivars (Rubus fructicosus L.). METHODS:\u0000Two blackberry cultivars (Thornfree and Boysen) were exposed to salt stress (20, 40, and 60 mM NaCl) for 28 days in in vitro culture. The photosynthetic activity was evaluated by probing chlorophyll a fluorescence (JIP test) and photosynthetic pigments. RESULTS:\u0000The maximum quantum yield of PSII (Fv/Fm), performance indexes (PIABS and PItotal), and photosynthetic driving force (DFABS) were greatly affected by salt stress in the leaves of Boysen cultivar. Lower photosynthetic efficiency was confirmed by the increased values of absorption flux per RC (ABS/RC), trapping flux per PSII RC (TR0/RC), dissipation flux per RC (DI0/RC), decreased values of chlorophyll and carotenoid contents, disruption in the interaction between the light-harvesting complex and the core proteins of PSII, as well as disruption of the oxygen-evolving complex. CONCLUSIONS:\u0000The provided informations suggest that the photosynthetic apparatus of Thornfree cultivar was found to be more tolerant to salt stress compared to Boysen.","PeriodicalId":15194,"journal":{"name":"Journal of Berry Research","volume":"32 1","pages":""},"PeriodicalIF":1.7,"publicationDate":"2023-12-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138680530","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Assessing genetic diversity of berberis genotypes selected from an F1 population using molecular markers","authors":"Amir Safari, Mehdi Rezaei","doi":"10.3233/jbr-230044","DOIUrl":"https://doi.org/10.3233/jbr-230044","url":null,"abstract":"Barberries are versatile shrubs with diverse applications, including ornamental, medicinal, and edible purposes. In this study, we employed molecular markers to assess the genetic diversity and genetic base of superior barberry genotypes selected from an F1 population obtained through Shahrood University Barberry Breeding Program (SUBBP), alongside their parents. We utilized nine ISSR markers and 10 RAPD markers to analyze the population’s genetic diversity. From these markers, we obtained 98 polymorphic bands using ISSR markers and 112 polymorphic bands using RAPD markers. The average PIC value was 0.16 for ISSR markers and RAPD markers, while the average genetic resolution power was 3.93 for ISSR markers and 2.11 for RAPD markers. Furthermore, we calculated the genetic dissimilarity coefficient (GDC) based on ISSR and RAPD markers, which ranged from 0.23 to 0.86 (average 0.62) and 0.21 to 0.85 (average 0.60), respectively. The ISSR data analysis classified the genotypes into three main clusters, with genotypes 0515, R5N1, ‘Bth’, ‘Seedless (BD)’, and R2N1 being genetically distant from the others. Similarly, the analysis of 10 RAPD primers resulted in the classification of genotypes into three main groups. Notably, genotype 0609 exhibited greater genetic distance from other genotypes in this subgroup. The Principal Coordinates Analysis (PCoA) using both ISSR and RAPD marker data further supported the grouping of genotypes into three distinct clusters. These results provide valuable insights into the genetic composition of the F1 population and contribute to the advancement of barberry breeding strategies.","PeriodicalId":15194,"journal":{"name":"Journal of Berry Research","volume":"3 1","pages":""},"PeriodicalIF":1.7,"publicationDate":"2023-11-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138539078","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comparative variability of nutrients, minerals, phenolics and anthocyanins with antioxidant potentials during fruit development stages in five Mulberry ( Morus) cultivars","authors":"Asma Saeed, Shabana Kauser, Adil Hussain, Abad Ali Nadeem","doi":"10.3233/jbr-230053","DOIUrl":"https://doi.org/10.3233/jbr-230053","url":null,"abstract":"BACKGROUND:Mulberry (Morus) from the Moraceae family is distributed widely in tropical to temperate regions of the world having economic and medicinal significance with exceptional nutritional characteristics. OBJECTIVES:Assessment of variations in sugars, acidity, minerals, total phenolics, anthocyanins and antioxidant potentials of three black mulberry (Morrus serrata, M. nigra and M. macroura black) and two white mulberry cultivars (M. alba and M. macroura white) during development stages. METHODS:Total sugars and acidity were evaluated with AOAC methods and total phenolic content was estimated with folin-ciocalteau method. Antioxidant activity was evaluated with DPPH assay, minerals were quantified with atomic absorption spectroscopy and anthocyanins were assessed and identified with high performance liquid chromatography and liquid chromatography-mass spectroscopy. RESULTS:Over all, M. alba (white cultivar) displayed maximum sugar (1.35%) at fruit development stage 3 (FDS-3) following M. serrata (black cultivar) at FDS-5 (1.1%) and M. macroura white (white cultivar) at FDS-4 (0.9%). Acidity decreased in M. alba (0.8–0.09%), M. nigra (0.8–0.03%) and M. macroura white (0.6–0.04%) during development. Total phenolic content was maximum (590 GAE/100 g) in M. nigra at FDS-6 and M. serrata (450 GAE/100 g) at FDS-7. Two black cultivars (M. serrata and M. nigra) showed significant increase in total anthocyanins (14.57–100.06 and 14.13–201.87 CGE/100 g FW) from FDS-1 to FDS-7 while decrease in M. macroura black and M. macroura white (17.07–11.79 and 9.03–2.68) from FDS-1 to FDS-7. The DPPH inhibition of black mulberries was pointedly greater with significant levels of iron, copper, zinc, calcium, magnesium, sodium, phosphorus and potassium than white cultivars during maturity. HPLC and LC-MS procedures identified various anthocyanins in all cultivars including cyanidin, malvidin, pelargonidin and delphinidin. CONCLUSIONS:Mulberries with improved nutrition, acceptable range of metals, bioactive compounds and higher antioxidant potentials could be significant against various diseases like cancer, cardiovascular problems, neurodegenerative disorders, obesity, diabetes and other metabolic syndromes.","PeriodicalId":15194,"journal":{"name":"Journal of Berry Research","volume":"163 1","pages":""},"PeriodicalIF":1.7,"publicationDate":"2023-11-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138539070","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}