Journal of Berry Research最新文献

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Black raspberry anthocyanins protect BV2 microglia from LPS-induced inflammation through down-regulating NOX2/TXNIP/NLRP3 signaling 黑覆盆子花青素通过下调NOX2/TXNIP/NLRP3信号通路,保护BV2小胶质细胞免受lps诱导的炎症
IF 1.7 4区 农林科学
Journal of Berry Research Pub Date : 2021-01-01 DOI: 10.3233/JBR-200692
Teng Mu, Y. Guan, Tianqiao Chen, Shuning Wang, Meifen Li, A. Chang, Zhe Yang, Xiuli Bi
{"title":"Black raspberry anthocyanins protect BV2 microglia from LPS-induced inflammation through down-regulating NOX2/TXNIP/NLRP3 signaling","authors":"Teng Mu, Y. Guan, Tianqiao Chen, Shuning Wang, Meifen Li, A. Chang, Zhe Yang, Xiuli Bi","doi":"10.3233/JBR-200692","DOIUrl":"https://doi.org/10.3233/JBR-200692","url":null,"abstract":"BACKGROUND: Increasing evidence has established neuroinflammation as the hallmark of neurodegenerative disorders such as Alzheimer’s disease (AD). However, despite the underlying immunological mechanisms are far from being understood, the involvement of excessive activation of microglia is attracting more and more attention. OBJECTIVE: In the present study, we investigated the protective effect of black raspberry (BRB) anthocyanins on LPS-induced neuroinflammation in BV2 microglia. METHODS: LPS-induced mouse BV2 microglia were treated with black raspberry anthocyanins and the levels of NO, ROS, IL-1β and IL-18 produced by the cells were measured to determine the extent of oxidative stress and inflammatory response. RESULTS: The results showed that BRB anthocyanins reduced the production of ROS in LPS-induced BV2 microglia by down-regulating the level of NOX2 and its downstream factors, including thioredoxin-interacting protein (TXNIP) and NOD-like receptor protein 3 (NLRP3) inflammasome. Furthermore, BRB anthocyanins inhibited the secretion of Interleukin-18 (IL-18) and Interleukin-1β (IL-1β), eventually attenuating the LPS-induced inflammatory response of BV2 microglia. CONCLUSIONS: BRB anthocyanins might play an important neuroprotective role in inflammation-related neurodegenerative disease, potentially, by down-regulating the NOX2 /TXNIP/ NLRP3 signaling axis in brain microglia.","PeriodicalId":15194,"journal":{"name":"Journal of Berry Research","volume":"11 1","pages":"333-347"},"PeriodicalIF":1.7,"publicationDate":"2021-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.3233/JBR-200692","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"70017836","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 4
Accumulation of sugars and associated gene expression in highbush blueberries differ by cultivar, ripening stage, and storage temperature 高丛蓝莓的糖积累和相关基因表达因品种、成熟期和贮藏温度而异
IF 1.7 4区 农林科学
Journal of Berry Research Pub Date : 2021-01-01 DOI: 10.3233/jbr-210748
Chau Thi Thu Nguyen, J. H. Lee, Phu-Tri Tran
{"title":"Accumulation of sugars and associated gene expression in highbush blueberries differ by cultivar, ripening stage, and storage temperature","authors":"Chau Thi Thu Nguyen, J. H. Lee, Phu-Tri Tran","doi":"10.3233/jbr-210748","DOIUrl":"https://doi.org/10.3233/jbr-210748","url":null,"abstract":"BACKGROUND: The sweetness of highbush blueberries is a sensory quality standard for consumers. Changes in metabolites and expression of associated genes induce variation in the sensory quality of blueberries. OBJECTIVE: This work investigated the ripening mechanism of blueberries by measuring changes in organic acids and sugar content as well as the gene expression levels associated with sugar accumulation, such as β amylase, invertase, sucrose phosphate synthase, and tonoplast monosaccharide transporter genes, before and after harvesting. METHODS: We used three different blueberry cultivars (Duke, Sierra, and Elizabeth) that we harvested at two ripening stages in a randomized complete block design and then kept at room temperature or 4°C for 7 days. The organic acids and sugar content were measured via HPLC, and gene expression levels were analyzed by qPCR. RESULTS: Cultivars, ripening stage, and storage conditions affected the synthesis of sugars and degradation of organic acids to determine the sweetness in blueberries and the expression of tagged genes and analyses of compounds involved in the metabolic mechanism. CONCLUSIONS: The results provide insights into the mechanism underlying the ripening and the postharvest quality. This study may support the selection of suitable blueberry cultivars that meet customer demand.","PeriodicalId":15194,"journal":{"name":"Journal of Berry Research","volume":"1 1","pages":""},"PeriodicalIF":1.7,"publicationDate":"2021-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.3233/jbr-210748","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"70019265","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 3
Factors affecting in vitro and in vivo antioxidant effects. Experimental conditions and nature of oxidants determine antioxidant efficacy 影响体外和体内抗氧化作用的因素。实验条件和氧化剂的性质决定了其抗氧化效果
IF 1.7 4区 农林科学
Journal of Berry Research Pub Date : 2021-01-01 DOI: 10.3233/JBR-200695
E. Niki
{"title":"Factors affecting in vitro and in vivo antioxidant effects. Experimental conditions and nature of oxidants determine antioxidant efficacy","authors":"E. Niki","doi":"10.3233/JBR-200695","DOIUrl":"https://doi.org/10.3233/JBR-200695","url":null,"abstract":"Reactive oxygen and nitrogen species have been implicated in the onset and progression of various diseases and the role of antioxidants in the maintenance of health and prevention of diseases has received much attention. The action and effect of antioxidants have been studied extensively under different reaction conditions in multiple media. The antioxidant effects are determined by many factors. This review aims to discuss several important issues that should be considered for determination of experimental conditions and interpretation of experimental results in order to understand the beneficial effects and limit of antioxidants against detrimental oxidation of biological molecules. Emphasis was laid on cell culture experiments and effects of diversity of multiple oxidants on antioxidant efficacy.","PeriodicalId":15194,"journal":{"name":"Journal of Berry Research","volume":"1 1","pages":"1-9"},"PeriodicalIF":1.7,"publicationDate":"2021-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.3233/JBR-200695","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"70017883","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 3
Is aromatic terpenoid composition of grapes in Northwestern Iberian wine cultivars related to variation in VviDXS1 gene? 伊比利亚西北部葡萄酒品种葡萄的芳香萜类成分是否与VviDXS1基因变异有关?
IF 1.7 4区 农林科学
Journal of Berry Research Pub Date : 2021-01-01 DOI: 10.3233/JBR-200609
C. Royo, P. Carbonell-Bejerano, R. Torres-Pérez, L. Freire, J. Ibáñez, J. Martínez-Zapater, M. Vilanova
{"title":"Is aromatic terpenoid composition of grapes in Northwestern Iberian wine cultivars related to variation in VviDXS1 gene?","authors":"C. Royo, P. Carbonell-Bejerano, R. Torres-Pérez, L. Freire, J. Ibáñez, J. Martínez-Zapater, M. Vilanova","doi":"10.3233/JBR-200609","DOIUrl":"https://doi.org/10.3233/JBR-200609","url":null,"abstract":"BACKGROUND: Monoterpenes and C13-norisoprenoids are key terpenoid compounds for wine aroma. The enzyme encoded by VviDXS1 participates in terpenoid biosynthesis in grapevine fruits and gain-of-function mutations in this gene lead to characteristic muscat aroma. OBJECTIVE: To assess for VviDXS1 contribution to aroma variation in Northwestern Iberian wine cultivars, we resequenced this gene in 111 cultivars and compared grape juice terpenic composition in 12 of them. METHODS: VviDXS1 was capture-targeted for resequencing with Illumina paired-end reads, SAMtools was used for variant calling and gene haplotypes were reconstructed with PHASE. Monoterpenes and C13-norisoprenoids were quantified in free and glycosidically-bound forms from grape juice by gas chromatography-mass spectrometry. RESULTS: Terpenic composition discriminated between muscat, terpenic and neutral profiles across cultivars. While the terpenic profile of Loureira and Albariño white cultivars was not associated with muscat-like mutations, Albariño carries a V34L substitution in VviDXS1 that is also present in other aromatic cultivars and was not reported before. Tempranillo and Cabernet Sauvignon red cultivars accumulated higher levels of C13-norisoprenoids, which was not associated with specific variation in VviDXS1. CONCLUSIONS: Apart from the uncharacterized substitution present in Albariño, findings suggest that terpenoid pathway-related genes other than VviDXS1 could contribute to the aromatic attributes of these cultivars.","PeriodicalId":15194,"journal":{"name":"Journal of Berry Research","volume":"1 1","pages":"1-14"},"PeriodicalIF":1.7,"publicationDate":"2021-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.3233/JBR-200609","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"70017350","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 1
Blueberries and insulin protect microglial cells against high glucose-induced inflammation and restore GLUT-1 蓝莓和胰岛素可以保护小胶质细胞免受高糖诱导的炎症,并恢复GLUT-1
IF 1.7 4区 农林科学
Journal of Berry Research Pub Date : 2021-01-01 DOI: 10.3233/JBR-200628
I. Hininger-Favier, N. Thangthaeng, D. Bielinski, D. Fisher, Shibu M. Poulose, B. Shukitt-Hale
{"title":"Blueberries and insulin protect microglial cells against high glucose-induced inflammation and restore GLUT-1","authors":"I. Hininger-Favier, N. Thangthaeng, D. Bielinski, D. Fisher, Shibu M. Poulose, B. Shukitt-Hale","doi":"10.3233/JBR-200628","DOIUrl":"https://doi.org/10.3233/JBR-200628","url":null,"abstract":"BACKGROUND: Growing evidence suggests that hyperglycemia could be harmful for cognitive function. That insulin (INS) has a neuro-modulatory role is supported by various findings, but its effect on microglia, the innate immune cells in the brain, is largely unknown. Blueberries have been shown to reduce neuro-inflammation. OBJECTIVE: We hypothesized that high glucose stimulated an inflammation in microglia and that BB and INS were able to reduce it and both might act through GLUT-1 transporter. METHODS: We examined the effects of low (5 mM), medium (25 mM), or high (50 mM) glucose, stimulated or not with lipopolysaccharide (LPS; 100 nM) with either BB extract (2 mg/ml) and/or INS, on inflammatory responses in a microglia cell line. Nitric oxide (NO) production and the expression levels of iNOS, TNF-α, NOX4 and glucose transporter protein-1 (GLUT1) were assessed. RESULTS: We observed that treatment with BB, similarly to INS treatments, reduced the high glucose concentration-induced response on oxidative stress and inflammation, and that this protective effect is more important with LPS added to glucose media. Interestingly, both BB and INS attenuated the LPS-induced inflammatory response on GLUT1. CONCLUSION: Increasing glucose concentration triggers inflammation by microglia. BB as well as INS protected microglia from high glucose levels, by reducing inflammation and altering glucose transport in microglia. These preliminary data compared for the first time BB to Insulin on microglia. Blueberries are promising dietary intervention to prevent diabetic neuropathy. Our preliminary results suggest a possible new mechanism involving GLUT-1 by which BB has insulin-like effects.","PeriodicalId":15194,"journal":{"name":"Journal of Berry Research","volume":"11 1","pages":"201-216"},"PeriodicalIF":1.7,"publicationDate":"2021-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.3233/JBR-200628","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"70017713","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 5
Vitis vinifera VvPUB17 functions as a E3 ubiquitin ligase and enhances powdery mildew resistance via the salicylic acid signaling pathway Vitis vinifera VvPUB17作为E3泛素连接酶,通过水杨酸信号通路增强白粉病抗性
IF 1.7 4区 农林科学
Journal of Berry Research Pub Date : 2021-01-01 DOI: 10.3233/JBR-210709
Min Li, Siqi Shen, Yi-bin Xing, W. Jiao, Yongzhong Zhan, Ya-dan Sun, D. Guo, Yi-He Yu
{"title":"Vitis vinifera VvPUB17 functions as a E3 ubiquitin ligase and enhances powdery mildew resistance via the salicylic acid signaling pathway","authors":"Min Li, Siqi Shen, Yi-bin Xing, W. Jiao, Yongzhong Zhan, Ya-dan Sun, D. Guo, Yi-He Yu","doi":"10.3233/JBR-210709","DOIUrl":"https://doi.org/10.3233/JBR-210709","url":null,"abstract":"BACKGROUND: Powdery mildew affects grapevine growth and development and reduces grapevine fruit yield and quality. Plant U-box (PUB) E3 ubiquitin ligases play important roles in ubiquitin/proteasome-mediated protein degradation during plant development and in the plant defense response. OBJECTIVE: We cloned the VvPUB17 gene from Vitis vinifera and analyzed that VvPUB17 enhanced the resistance of grapevine to powdery mildew through the SA signal pathway. METHODS: Pathogen inoculation of Arabidopsis thaliana and grapevine plants was carried out by the tableting method. Gene expression was analyzed by quantitative real-time PCR. Sequence analysis and in vitro ubiquitination experiments show the structure and characteristics of VvPUB17. Exogenous salicylic acid, methyl jasmonate, ethylene and powdery mildew induced the expression of VvPUB17 in Arabidopsis leaves to verify the resistance of VvPUB17 to powdery mildew. RESULTS: Sequence analysis and in vitro ubiquitination experiments show that VvPUB17 contains U-box and Armadillo repeats (ARM repeat) and has E3 ubiquitin ligase activity dependent on the conserved U-box motif. Transgenic plants showed elevated levels of key genes related to the SA defense response pathway and high concentrations of salicylic acid. CONCLUSIONS: VvPUB17 functions as an E3 ubiquitin ligase that enhances the resistance of grapes to powdery mildew through the SA signal pathway.","PeriodicalId":15194,"journal":{"name":"Journal of Berry Research","volume":"1 1","pages":""},"PeriodicalIF":1.7,"publicationDate":"2021-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.3233/JBR-210709","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"70018902","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Hawthorn berry (crataegus songarica) causes endothelium-dependent relaxation of the porcine coronary artery: Role of Estrogen receptors 山楂引起猪冠状动脉内皮依赖性松弛:雌激素受体的作用
IF 1.7 4区 农林科学
Journal of Berry Research Pub Date : 2021-01-01 DOI: 10.3233/JBR-200662
Waqas Younis, Alamgeer, V. Schini-Kerth, M. Farooq, Musaad Althobaiti, R. Roberts
{"title":"Hawthorn berry (crataegus songarica) causes endothelium-dependent relaxation of the porcine coronary artery: Role of Estrogen receptors","authors":"Waqas Younis, Alamgeer, V. Schini-Kerth, M. Farooq, Musaad Althobaiti, R. Roberts","doi":"10.3233/JBR-200662","DOIUrl":"https://doi.org/10.3233/JBR-200662","url":null,"abstract":"BACKGROUND: Fruits of Crataegus songarica are commonly used for the treatment of vascular insufficiency and heart problems. OBJECTIVE: Our aim was to determine the effect of C. songarica on vascular tone and to determine the mechanisms underlying the vasorelaxant properties. METHODS: Extracts of C. songarica were tested for vasodilator activity of porcine coronary artery after pre-contraction with the thromboxane mimetic U46619 in the presence or absence of inhibitors of intracellular signaling cascades. Reactive oxygen species were assessed by dihydroethidine staining and the level of eNOS and AKT phosphorylation was measured by immunohistochemical staining. RESULTS: Extracts of C. songarica berries produced endothelium dependent vasorelaxation, with most significant effect induced by aqueous fraction (AS-CS). This vasorelaxant effect of AS-CS was reduced by inhibition of nitric oxide pathways and inhibition of potassium channels. Inhibition of phosphatidylinositol 3- kinase and Src tyrosine kinase, as well as scavenging of reactive oxygen species, produced an attenuation of the relaxation response. Estrogen receptor antagonists (tamoxifen and ICI 182,782) reduced the AS-CS mediated vasorelaxation. AS-CS also stimulated the endothelial formation of ROS and phosphorylation of Akt and eNOS. CONCLUSION: The data indicated that C. songarica produces an endothelium-dependent vasorelaxation, which is partly dependent upon estrogen receptors, and sensitive to inhibition of ROS/Src/PI3K/NO pathways.","PeriodicalId":15194,"journal":{"name":"Journal of Berry Research","volume":"1 1","pages":"1-17"},"PeriodicalIF":1.7,"publicationDate":"2021-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.3233/JBR-200662","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"70018115","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
The treatment with an extract from Calafate (Berberis microphylla) induces transcript and protein expression of molecules involved in thermogenesis and adipocyte browning in adipose tissue from obese mice 用小叶小檗提取物处理可诱导肥胖小鼠脂肪组织中参与产热和脂肪细胞褐变的分子的转录和蛋白质表达
IF 1.7 4区 农林科学
Journal of Berry Research Pub Date : 2021-01-01 DOI: 10.3233/JBR-200670
L. Duarte, J. Quezada, L. A. Ramirez, Karla Vásquez, J. Orellana, V VanessaVillanueva, F. Pérez-Bravo, D. Garcia-Diaz
{"title":"The treatment with an extract from Calafate (Berberis microphylla) induces transcript and protein expression of molecules involved in thermogenesis and adipocyte browning in adipose tissue from obese mice","authors":"L. Duarte, J. Quezada, L. A. Ramirez, Karla Vásquez, J. Orellana, V VanessaVillanueva, F. Pérez-Bravo, D. Garcia-Diaz","doi":"10.3233/JBR-200670","DOIUrl":"https://doi.org/10.3233/JBR-200670","url":null,"abstract":"BACKGROUND: Polyphenols intake increases the function of brown adipose tissue (BAT), stimulating energy expenditure (EE). Calafate (Berberis microphylla) is a polyphenol-rich Chilean native fruit. OBJECTIVE: To analyse the effect of a treatment with a Calafate extract in the thermogenic activity of mice adipose tissues. METHODS: Forty adult C57BL/6J male mice were subdivided into four groups (n=10 each): control diet, control+Calafate (extract: 50mg total polyphenols/kg weight), high-fat diet (HF) and HF+Calafate. RESULTS: Calafate prevented the increase in body weight and the decrease EE induced by HF. In BAT, Ucp-1 transcript was influenced by the interaction between diet and Calafate (p<0.01), Pparα showed the same expression pattern as Ucp-1 and both, diet (p<0.01) and Calafate (p<0.05), induced significant effects in Sirt1. In inguinal adipose tissue, Pgc1α, Pparα, Prdm16, Sirt1, and Dio2 transcripts presented a decreased expression caused by HF, that was reversed by Calafate. In BAT, an effect of diet (p<0.05) and an interaction between diet and Calafate (p<0.01) was observed in UCP-1 protein levels. CONCLUSIONS: A treatment with Calafate drives less weight gain in mice fed with HF, and reverses the effects generated by it on the expression of thermogenic and browning markers.","PeriodicalId":15194,"journal":{"name":"Journal of Berry Research","volume":"38 1","pages":"1-11"},"PeriodicalIF":1.7,"publicationDate":"2021-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.3233/JBR-200670","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"70018206","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 2
Effects of photoselective colour nets on the vegetative, productive, and qualitative behaviour of kiwifruit, jintao cultivar 光选择性色网对猕猴桃营养、生产和品质行为的影响
IF 1.7 4区 农林科学
Journal of Berry Research Pub Date : 2021-01-01 DOI: 10.3233/jbr-200530
G. Gullo, A. Dattola, V. Vonella, R. Zappia
{"title":"Effects of photoselective colour nets on the vegetative, productive, and qualitative behaviour of kiwifruit, jintao cultivar","authors":"G. Gullo, A. Dattola, V. Vonella, R. Zappia","doi":"10.3233/jbr-200530","DOIUrl":"https://doi.org/10.3233/jbr-200530","url":null,"abstract":"BACKGROUND: In the Mediterranean basin, the solar radiation received is very high for many tree species over several hours of the day during the summer. Using a photoselective net can improve the climatic conditions and also modify the quality of light received. OBJECTIVE: This study aimed to evaluate the influence of the colour of photoselective nets on the yield and quality of Actinidia chinensis. MATERIALS: The experiment was carried out in Southern Italy on an orchard of Jintao kiwifruit. Photoselective nets of different colours were used: black, red, yellow, white, and grey. The resulting fruits were compared to those of plants in the open field. A randomised block design was adopted. Environmental, vegetative, leaf gas exchange parameter, yield, and fruit quality were defined. All data were analysed using the Variance Analysis (ANOVA) and the Principal Component Analysis (PCA). RESULTS: Photoselectivity influenced production. The best results were obtained under the red net in terms of production and fertility indices. Fruit size, maturation indices, and nutraceutical parameters were also higher under the red net. Among the nets, the grey net induced the worst tree productivity results and the worst fruit quality parameters. However, all nets showed better results compared to the open field. CONCLUSION: The photoselective net strongly influenced the yield and quality of Jintao kiwifruit and the better results compared to the open field. The red net proved to be the best performer for the environment where the experiment was carried out.","PeriodicalId":15194,"journal":{"name":"Journal of Berry Research","volume":"11 1","pages":"1-19"},"PeriodicalIF":1.7,"publicationDate":"2021-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.3233/jbr-200530","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"70016986","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 6
Genome-wide identification and expression analysis revealed cinnamyl alcohol dehydrogenase genes correlated with fruit-firmness in strawberry 全基因组鉴定和表达分析显示,肉桂醇脱氢酶基因与草莓果实硬度相关
IF 1.7 4区 农林科学
Journal of Berry Research Pub Date : 2021-01-01 DOI: 10.3233/jbr-210729
Ping Wang, Jing Yang, Zi-Yi Li, Jia-Jun Zhu, Gao Qinghua, D. Ni, Duan Ke
{"title":"Genome-wide identification and expression analysis revealed cinnamyl alcohol dehydrogenase genes correlated with fruit-firmness in strawberry","authors":"Ping Wang, Jing Yang, Zi-Yi Li, Jia-Jun Zhu, Gao Qinghua, D. Ni, Duan Ke","doi":"10.3233/jbr-210729","DOIUrl":"https://doi.org/10.3233/jbr-210729","url":null,"abstract":"BACKGROUND: Strawberry fruits are perishable with a short post-harvest life. Cinnamyl alcohol dehydrogenase (CAD) is the key enzyme for lignin biosynthesis strengthening plant cell wall. A systematic characterization of strawberry CAD family is absent and their involvement in fruit firmness is largely elusive. OBJECTIVE: Current work aims for a genome-wide identificationof CAD family and its expression correlation with fruit firmness in strawberry varieties. METHODS: A genome-scale identification and molecular characterization for CADs were performed in the commercial strawberry (Fragaria×ananassa) and woodland strawberry (F. vesca). qPCR analysis of CAD homoeologs in three cultivars varying with fruit firmness revealed candidate CAD members positively correlated with lignin content and fruit firmness. RESULTS: A total of 14 and 24 CAD loci were identified in the genomes of F.vesca var. Hawaii4 and F. ×ananassa cv. Camarosa, respectively.Phylogenetic analysis supported a division of this family into three classes. Class I FvCAD each has four homoeologs in commercial strawberry, while those of Class II and Class III have only one or two homoeologs. Except for FvCAD2 and -6, there exits at least one pair of CADs sharing ∼97% or above amino acid identity between F. vesca and F. ×ananassa.The flesh firmness and lignin content varied greatly among strawberry germplasm. Distinct dynamic changes in fruit lignin content were observed before the large green stage, but fruit firmness displayed a similar decrease profile during fruit development in three varieties. Of the eight genes detected in F.×ananassa, FvCAD3 and -12 did not display a F. vesca-biased expression pattern during fruit development.FvCAD4 of Class I was expressed at levels positively correlated with variation in fruit lignin content at white stage.Transcript abundance of five Class IIgenes including FvCAD3, -8, -10, -11, and -12 was positively correlated with lignin content and fruit firmness, with FvCAD10 and -11 (FaCAD in previous publication) reaching an extremely significant correlation with the genetic variation in fruit firmness across three varieties. CONCLUSION: Strawberry Class II CADs were significantly correlated with the genetic variation in fruit firmness, which might expand the potential choices for improving strawberry shelf life.","PeriodicalId":15194,"journal":{"name":"Journal of Berry Research","volume":"1 1","pages":""},"PeriodicalIF":1.7,"publicationDate":"2021-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.3233/jbr-210729","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"70019236","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 5
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