Journal of Biosciences最新文献_第5页

O-GlcNAc transferase promotes synaptic assembly independent of catalytic activity in C. elegans. O-GlcNAc转移酶在秀丽隐杆线虫中独立于催化活性促进突触组装。

IF 2.1 4区生物学

Journal of Biosciences Pub Date : 2025-01-01

Mengting Wu, Huihui Jiang, Qian Li, Yunhe Liu, Hongjun Zhang, Zhiyong Shao

引用次数: 0

Human adipose-derived stem cell exosomes alleviate human respiratory system-related cells damaged by exposure to SO₂ derivatives. 人类脂肪来源的干细胞外泌体减轻暴露于二氧化硫衍生物的人类呼吸系统相关细胞的损伤。

IF 2.1 4区生物学

Journal of Biosciences Pub Date : 2025-01-01

Ji-Seon Lee, You-Rin Kim, Dogeon Yoon, Ji Hye Park, Ga Eun You, Wook Chun

{"title":"Human adipose-derived stem cell exosomes alleviate human respiratory system-related cells damaged by exposure to SO2 derivatives.","authors":"Ji-Seon Lee, You-Rin Kim, Dogeon Yoon, Ji Hye Park, Ga Eun You, Wook Chun","doi":"","DOIUrl":"","url":null,"abstract":"Inhalation burns, especially when combined with thermal burns, can be fatal and significantly increase mortality rate through inhaling hazardous gas. However, there is no specific treatment for inhalation burns except for relieving bronchospasm and cleaning the airways. In particular, inhaled sulfur dioxide (SO2), a major component of inhalation burns, can easily be hydrated in the respiratory tract to produce sulfurous acid, which subsequently dissociates to form bisulfite and sulfite derivatives. In this study, we intend to assess whether human adipose-derived stem cell (ASC) exosomes rescue respiratory system-related cells damaged by exposure to SO2 derivatives. We found that the uptake of ASC exosomes was high in human respiratory systemrelated cells and they rescue decreased proliferation of cells damaged by treatment with SO2 derivatives. In human pulmonary endothelial cells (HPMECs), total tubule length was increased by pre-treatment of ASC exosomes through an in vitro angiogenesis assay. Besides, we confirmed that ASC exosomes alleviate increased expression of inflammation-related genes by treatment of SO2 derivatives in primary respiratory epithelial cells. Taken together, these results suggest that ASC exosomes have potential in regeneration of human respiratory system-related cells damaged by inhalation burns, which currently lack specific treatment methods.","PeriodicalId":15171,"journal":{"name":"Journal of Biosciences","volume":"50 ","pages":""},"PeriodicalIF":2.1,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144325831","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Respiratory viruses and systemic lupus erythematosus: Biomarkers and mechanisms leading to autoimmunity. 呼吸道病毒和系统性红斑狼疮：导致自身免疫的生物标志物和机制。

IF 2.1 4区生物学

Journal of Biosciences Pub Date : 2025-01-01

Aruna Rajalingam, Sekar Kanagaraj, Anjali Ganjiwale

{"title":"Respiratory viruses and systemic lupus erythematosus: Biomarkers and mechanisms leading to autoimmunity.","authors":"Aruna Rajalingam, Sekar Kanagaraj, Anjali Ganjiwale","doi":"","DOIUrl":"","url":null,"abstract":"Autoimmunity has been explored in various viral infections, and its relevance to respiratory viruses deserves more attention, especially its immune derangement during these infections, which could potentially trigger relapse and induction of many new cases. Our study aimed to utilize publicly available transcriptomic respiratory viral datasets of rhinovirus, influenza virus, respiratory syncytial virus, and COVID-19 to understand their autoimmune activation. Antibodies produced against the autoantigens associated with respiratory viruses resulted in the identification of three biomarker genes: TRIM21, ELANE, and CTSG. These genes are reported to be involved in the pathways of neuroactive ligand-receptor interaction, neutrophil extracellular trap formation, apoptosis, amebiasis, renin-angiotensin system, and lysosome, commonly triggering the systemic lupus erythematosus (SLE) pathway in genetically susceptible SLE patients. These results emphasize that the key genes are enriched mainly in the immune system process linking SLE pathogenesis. Literature sources suggest that the biomarkers induce autoreactivity through bystander activation and molecular mimicry which results in aberrant B-cell activation and the formation of neutrophil extracellular traps leading to autoimmunity. Thus, these key biomarkers indicate a new direction for early diagnosis, risk assessment, and treatment of respiratory virus infections and SLE pathogenesis.","PeriodicalId":15171,"journal":{"name":"Journal of Biosciences","volume":"50 ","pages":""},"PeriodicalIF":2.1,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144674894","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Life history traits and spatial characteristics of an Asia Minor thin-toed gecko population (Mediodactylus heterocercus Blanford, 1874). 小亚细亚瘦趾壁虎种群生活史特征和空间特征（Mediodactylus heterocercus Blanford, 1874）。

IF 1.9 4区生物学

Journal of Biosciences Pub Date : 2025-01-01

Cantekin Dursun, Kamil Candan, Elif YıLDıRıM Caynak, Yusuf Kumlutaş, Çetin Ilgaz, Serkan Gül

{"title":"Life history traits and spatial characteristics of an Asia Minor thin-toed gecko population (Mediodactylus heterocercus Blanford, 1874).","authors":"Cantekin Dursun, Kamil Candan, Elif YıLDıRıM Caynak, Yusuf Kumlutaş, Çetin Ilgaz, Serkan Gül","doi":"","DOIUrl":"","url":null,"abstract":"The aim of the present study was to obtain information on certain life history traits such as life span and age of sexual maturity of individuals of the species Mediodactylus heterocercus using the method of skeletochronology. A total of 31 specimens (12 males, 19 females) was aged from a population located in Güzelköy (Haliliye, Şanlıurfa, Türkiye). The age of male individuals ranged from 4 to 9 years, while that of females ranged from 3 to 10 years, with mean values of 6.25±1.60 and 5.21±1.55 years, respectively. The age of sexual maturity was 3 years for both sexes. The mean age of individuals in the population did not show a statistically significant difference between the sexes. The calculated mean values of snout-vent length (SVL) were 41.90±4.31 mm (33.53-46.69 mm) for males and 39.16±5.05 mm (30.18-48.29 mm) for females. It was concluded that the difference in SVL measurements between the sexes was not statistically significant, but a significant relationship between age and SVL was found. The species M. heterocercus does not follow Bergmann's rule with regard to climatic or topographic heterogeneity.","PeriodicalId":15171,"journal":{"name":"Journal of Biosciences","volume":"50 ","pages":""},"PeriodicalIF":1.9,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145137611","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Analysis of downstream targets of PAX6 and LHX2, fundamental regulators of the developing mammalian neocortex. 哺乳动物新皮层发育的基本调控因子PAX6和LHX2的下游靶点分析。

IF 2.1 4区生物学

Journal of Biosciences Pub Date : 2025-01-01

Mansi Srivastava, Varun Suresh, Shubha Tole

{"title":"Analysis of downstream targets of PAX6 and LHX2, fundamental regulators of the developing mammalian neocortex.","authors":"Mansi Srivastava, Varun Suresh, Shubha Tole","doi":"","DOIUrl":"","url":null,"abstract":"The transcription factors PAX6 and LHX2 play fundamental roles in mammalian cerebral cortical development. Loss of either factor causes depletion of the cortical progenitor pool and reduction of neurogenesis of later-born cortical neurons. We compared the chromatin occupancy of PAX6 and LHX2 and transcriptional dysregulation upon loss of each factor to analyze whether they function via common gene regulatory networks. We identified common direct and indirect targets that were dysregulated either concordantly or discordantly, based on whether their expression showed similar up- or downregulation upon loss of either factor. Finally, we examined single-cell RNA-seq datasets from neocortical progenitors to identify the cell types within which each common direct/indirect and concordantly/discordantly regulated target gene is expressed as cortical progenitors undergo neurogenesis. Our analysis shows that PAX6 and LHX2 have several common targets that suggest similar pathways for progenitor maintenance, but the regulation of neurogenesis may occur via at least partially non-overlapping pathways. Furthermore, each factor functions to suppress the expression of a set of common Cajal-Retzius cell-specific and interneuron-specific genes which are not normally expressed by cortical progenitors. Together, our analysis offers experimentally testable hypotheses for how PAX6 and LHX2 may execute their critical roles.","PeriodicalId":15171,"journal":{"name":"Journal of Biosciences","volume":"50 ","pages":""},"PeriodicalIF":2.1,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143255552","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A jack of all trades: Hermaphrodite-specific serotonergic neuron in C. elegans. 多面手：秀丽隐杆线虫的雌雄同体特异性血清素能神经元。

IF 2.1 4区生物学

Journal of Biosciences Pub Date : 2025-01-01

Patrick Laurent

{"title":"A jack of all trades: Hermaphrodite-specific serotonergic neuron in C. elegans.","authors":"Patrick Laurent","doi":"","DOIUrl":"","url":null,"abstract":"How does a single neuron type shape both short- and long-term behavior? A study reveals how co-transmission by two hermaphrodite-specific neurons (HSNs) of Caenorhabditis elegans produces both transient and long-lasting effects on its egg-laying behavior. A key question in neuroscience is how neurons and circuits integrate information to drive behavior. In the nematode C. elegans, locomotion, feeding, defecation and egg-laying motor programs are all generated by a simple nervous system consisting of 302 neurons whose connectivity patterns are fully described (White et al. 1986). Egg-laying behavior allows dispersal of eggs in the most appropriate locations. The egg-laying circuit consists of two hermaphrodite-specific serotonergic neurons (HSNs) and six ventral cholinergic C-neurons (VCs) connected to the vulval muscle. In addition, uv1 neuroendocrine cells mechanically respond to egg laying by the release of tyramine and neuropeptides. Together, they produce a rhythmic behavior modulated by environmental cues that alternates between egg-laying phases and resting periods (Hardaker et al. 2001; Ringstad and Horvitz 2008; Fenk and de Bono 2015). How can such a simple circuit generate rhythmic and regulated behavior?","PeriodicalId":15171,"journal":{"name":"Journal of Biosciences","volume":"50 ","pages":""},"PeriodicalIF":2.1,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144000971","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Ex vivo expansion of human disc chondrocytes from surgically removed disc tissue: Post-operative tissue stability. 人椎间盘软骨细胞从手术切除的椎间盘组织中体外扩增：术后组织稳定性。

IF 2.1 4区生物学

Journal of Biosciences Pub Date : 2025-01-01

Ankita Bendre, Rohini Lad, Sharwani Dole, Saurabh Vaishnav, Onkar Sudame, Sunil Nadkarni, Rita Mulherkar

{"title":"Ex vivo expansion of human disc chondrocytes from surgically removed disc tissue: Post-operative tissue stability.","authors":"Ankita Bendre, Rohini Lad, Sharwani Dole, Saurabh Vaishnav, Onkar Sudame, Sunil Nadkarni, Rita Mulherkar","doi":"","DOIUrl":"","url":null,"abstract":"Cell therapy using autologous, ex vivo expanded chondrocytes from surgically removed disc tissues has emerged as a promising treatment option for patients with degenerated intervertebral discs. One critical aspect of this is the requirement to culture cells in a good manufacturing practice (GMP)-compliant tissue culture facility. Given the potential for delays during the transportation of disc tissue to a centralized cell culture facility, our study aimed to assess the stability of surgically removed disc tissues stored appropriately for varying durations. Surgically removed disc tissues, stored in normal saline at 4°C for different durations, were cultured in vitro in Dulbecco's Modified Eagle medium (DMEM) supplemented with human platelet lysate. Immunophenotyping was performed using a panel of reported chondrocyte-specific antibodies, including cluster of differentiation 73 (CD73), CD90, CD105, CD166, CD14, human leukocyte antigen DR (HLA-DR), and CD34, for validation. We report that more than 80% of the tissues remained viable up to the 24-h time point as evidenced by cell growth, after which the success of culturing the chondrocytes fell to almost 30% when stored for up to 96 h. The immunophenotype of the cells was identical at all time points. For cell therapy, surgically removed disc tissue should preferably be expanded ex vivo within 24 h post surgery, although all successfully cultured cells, irrespective of the storage duration, expressed the same phenotypic markers. This study will help in planning autologous chondrocyte cell therapy for back pain where the tissue has to be transported to distant GMP facilities.","PeriodicalId":15171,"journal":{"name":"Journal of Biosciences","volume":"50 ","pages":""},"PeriodicalIF":2.1,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144275019","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Chronic administration of morphine provokes generation of antibodies to morphine and immunosuppression in individuals with opioid use disorder. 长期服用吗啡可引起阿片类药物使用障碍患者产生吗啡抗体和免疫抑制。

IF 2.1 4区生物学

Journal of Biosciences Pub Date : 2025-01-01

Sidhanta Nanda, Mohammad Adeel Zafar, Sanpreet Singh, Jonaid Ahmad Malik, Ritika Gautam, Abhishek Ghosh, Debasish Basu, Javed N Agrewala

{"title":"Chronic administration of morphine provokes generation of antibodies to morphine and immunosuppression in individuals with opioid use disorder.","authors":"Sidhanta Nanda, Mohammad Adeel Zafar, Sanpreet Singh, Jonaid Ahmad Malik, Ritika Gautam, Abhishek Ghosh, Debasish Basu, Javed N Agrewala","doi":"","DOIUrl":"","url":null,"abstract":"Globally, opioid use disorder (OUD) presents a significant public health challenge linked to high mortality and disability rates. Heroin and other morphine derivatives are prevalent among abused opioids. Prolonged exposure to these substances in individuals with OUD can trigger an immune response, leading to the production of antibodies to morphine that may bind to morphine and potentially mitigate its rewarding effects. In our study, we analyzed serum samples from patients diagnosed with OUD to explore the nature and properties of antibodies to morphine, aiming to characterize the generation of antibodies to morphine due to long-term exposure to morphine. We observed varying titers of antibodies to morphine in OUD patients, absent in healthy controls, with both free morphine and morphine complexes detected bound to these antibodies, indicating less potency. Furthermore, our analysis revealed elevated levels of FoxP3, a critical transcription factor in regulatory T-cells (Tregs) responsible for maintaining immunosuppression. Concurrently, reduced levels of inducible nitric oxide synthase (iNOS) and interleukin-6 (IL-6) indicated immunosuppressive activity. Notably, decreased antibody titers against the Acr1 protein of Mycobacterium tuberculosis suggested that morphine-induced immune suppression might compromise responses to other pathogens. These findings indicate that chronic morphine exposure not only suppresses host immunity but also induces the production of antibodies to morphine. Investigating whether these antibodies contribute to immune suppression or can be harnessed to combat morphine dependence presents an intriguing avenue for future research.","PeriodicalId":15171,"journal":{"name":"Journal of Biosciences","volume":"50 ","pages":""},"PeriodicalIF":2.1,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144275023","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Decoding hepatitis B virus mutations that impact host-virus interactions and therapeutics. 解码影响宿主-病毒相互作用和治疗的乙型肝炎病毒突变。

IF 2.1 4区生物学

Journal of Biosciences Pub Date : 2025-01-01

Harshni Venkatesan, Jagadeesh Sai Mahesh, Sangita Venkataraman

{"title":"Decoding hepatitis B virus mutations that impact host-virus interactions and therapeutics.","authors":"Harshni Venkatesan, Jagadeesh Sai Mahesh, Sangita Venkataraman","doi":"","DOIUrl":"","url":null,"abstract":"Hepatitis B virus (HBV), a lethal virus that results in the loss of two lives every minute, induces chronic and acute infections. Chronic infections may result in liver cirrhosis, which in turn may lead to hepatocellular carcinoma (HCC). Our study analysed 1,06,970 protein sequences of HBV genotypes (Gen A to H) from the HBV database (HBVdb) to construct position-specific scoring matrices. A total of 5,058 mutations were detected across all proteins, reflecting the notorious mutability of HBV. Among these, 2,658 significant mutations (sigmuts) with frequencies ranging between 10 and 80 were screened. Gen A presented the greatest number of sigmuts, whereas Gen H presented the least. Gen C, the most common HBV gene, featured 417 sigmuts, which we used for structural studies using DynaMut2 and molecular docking. We found that most core protein signatures significantly impact functions, including B-cell receptor binding and dimerisation. Interestingly, most sigmuts of the RNase H domain (694-843) of polymerase proteins promoted structural disorder, with possible impact on interactions with LINE-1 elements and progression to hepatocellular carcinoma. Intriguingly, despite the use of prominent nucleoside reverse transcriptase inhibitors (NRTIs) for over two decades, the drug-binding pockets of polymerase proteins have been found to be highly conserved. Nevertheless, since the long-term use of a few drugs as monotherapies has resulted in the development of drug resistance in recent years, we propose novel HBV targets for alternative therapeutic interventions.","PeriodicalId":15171,"journal":{"name":"Journal of Biosciences","volume":"50 ","pages":""},"PeriodicalIF":2.1,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144674892","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Role of the extracellular matrix in amylin aggregation: Opportunities for improved therapy in type 2 diabetes mellitus. 细胞外基质在胰淀素聚集中的作用：改善2型糖尿病治疗的机会

IF 1.9 4区生物学

Journal of Biosciences Pub Date : 2025-01-01

Sagarika Prabhakar, Priyadarshini Veerabhadraswamy, Nikhil R Gandasi

引用次数: 0