Impact of HIV-1 TAT activation on retinal Müller glia: Implications for barrier properties.

IF 2.1 4区 生物学 Q2 BIOLOGY
Journal of Biosciences Pub Date : 2025-01-01
Kamini Khatak, Kavitha Sankaranarayanan, Nivedita Chatterjee
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引用次数: 0

Abstract

HIV-associated immune activation is characterized by an increase in pro-inflammatory mediators and dysfunctional T-cells with senescent phenotypes. This persistent activation predisposes HIV-infected persons to non-AIDS-defining co-morbid conditions. At the retina, Müller glia undertake innate immune functions. Evidence from our microarray data shows changes in pathways which include cytokines, their receptors, and focal adhesion genes, suggesting inflammatory changes which could affect the blood-retinal barrier. Using a bioinformatics approach, we analyzed our dataset to identify changes in reactive Müller glia. Abnormalities in Müller glia signaling involve phosphatidylinositol 3-kinase (PI3K) and protein kinase B (AKT). In silico analysis was validated by quantitative RT-PCR. PKB/AKT is increased in reactive Müller glia. Inhibition of PI3K/AKT affected transendothelial resistance in TAT-exposed Müller glia. Identification of a cluster of gene expression suggests underlying changes in the functions of Müller glia in maintaining barrier permeability through the PI3K/AKT signaling network. Activation of retinal Müller cells can therefore lead to proinflammatory molecular cascades that promote widespread physiological changes. Alterations in these pathways may affect vascular permeability, retinal and corneal angiogenesis, and disruption of the blood-ocular barrier.

HIV-1 TAT激活对视网膜神经胶质细胞的影响:对屏障特性的影响。
hiv相关免疫激活的特征是促炎介质的增加和衰老表型的功能失调t细胞。这种持续的激活使艾滋病毒感染者易患非艾滋病定义的合并症。在视网膜上,神经胶质承担先天免疫功能。来自微阵列数据的证据显示,包括细胞因子、它们的受体和局灶粘附基因在内的通路发生了变化,表明炎症变化可能影响血液-视网膜屏障。使用生物信息学方法,我们分析了我们的数据集,以确定反应性勒神经胶质细胞的变化。突触神经胶质信号异常涉及磷脂酰肌醇3-激酶(PI3K)和蛋白激酶B (AKT)。通过定量RT-PCR验证硅分析。PKB/AKT在反应性勒胶质细胞中升高。抑制PI3K/AKT影响tat暴露的神经胶质细胞的跨内皮耐药。一组基因表达的鉴定表明,在通过PI3K/AKT信号网络维持屏障通透性的过程中,突触神经胶质的功能发生了潜在的变化。因此,视网膜网膜细胞的活化可导致促炎分子级联反应,从而促进广泛的生理变化。这些通路的改变可能影响血管通透性、视网膜和角膜血管生成以及血眼屏障的破坏。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
Journal of Biosciences
Journal of Biosciences 生物-生物学
CiteScore
5.80
自引率
0.00%
发文量
83
审稿时长
3 months
期刊介绍: The Journal of Biosciences is a quarterly journal published by the Indian Academy of Sciences, Bangalore. It covers all areas of Biology and is the premier journal in the country within its scope. It is indexed in Current Contents and other standard Biological and Medical databases. The Journal of Biosciences began in 1934 as the Proceedings of the Indian Academy of Sciences (Section B). This continued until 1978 when it was split into three parts : Proceedings-Animal Sciences, Proceedings-Plant Sciences and Proceedings-Experimental Biology. Proceedings-Experimental Biology was renamed Journal of Biosciences in 1979; and in 1991, Proceedings-Animal Sciences and Proceedings-Plant Sciences merged with it.
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