Journal of biotechnology & biomaterials最新文献_第3页

Glycosylation of therapeutic proteins: Functions and analytical innovation 治疗蛋白的糖基化:功能和分析创新

Journal of biotechnology & biomaterials Pub Date : 2017-12-14 DOI: 10.4172/2155-952X-C1-081

Baolin Zhang

引用次数: 0

DNA Barcoding of Tilapia Species (Pisces: Cichlidae) from North-Eastern Nigeria 尼日利亚东北部罗非鱼种(双鱼座:慈鱼科)的DNA条形码

Journal of biotechnology & biomaterials Pub Date : 2017-11-21 DOI: 10.4172/2155-952X.1000277

O. A. Sogbesan, M. Sanda, J. Ja’afar, Hameed Adebowale Adedeji

{"title":"DNA Barcoding of Tilapia Species (Pisces: Cichlidae) from North-Eastern Nigeria","authors":"O. A. Sogbesan, M. Sanda, J. Ja’afar, Hameed Adebowale Adedeji","doi":"10.4172/2155-952X.1000277","DOIUrl":"https://doi.org/10.4172/2155-952X.1000277","url":null,"abstract":"The Tilapiine complexes are made up of diverse species, hence the need for proper identification. Samples of tilapia species belonging to the family of Cichlidae obtained from Upper Benue River and Lake Geriyo landing sites were used for this study. Fish species were morphologically identified in situ by visual inspection with the aid of field guide for taxonomic studies. DNA was extracted from fresh fish tissue and the barcode gene region cytochrome c oxidase subunit 1 (co1) was amplified using the FishF1 and FishR1 primer pair. Amplified products were visualized on 1% agarose gel electrophoresis, purified and sequenced using Genetic Analyzer (Applied Biosystems 3130 XL). Four species (Oreochromis niloticus, Sarotherodon galilaeus, Sarotherodon galilaeus boulengeri and Coptodon zillii) belonging to three genera (Oreochromis, Sarotherodon and Coptodon) were identified in the study area. From our sampling survey, Oreochromis niloticus was the most dominant species in both areas, followed by Coptodon zillii. Sarotherodon spp. was not present in Upper Benue River but present in Lake Geriyo (an ox-bow lake of Upper Benue River). A new sub-species of Sarotherodon galilaeus known as Sarotherodon galilaeus boulengeri was identified in this study from the sequencing result. This species has never been reported in any literature to be present in Nigeria, which further confirmed the efficacy of DNA barcoding in the identification of cryptic species. The phylogenetic relationship and genetic distance between generic groups obtained in this study was in concordance with the hypothesis of Trewavas that the mouth brooders Orechromis niloticus and Sarotherodon galilaeus are more closely related to each other than the substrate brooders (Tilapia zillii).","PeriodicalId":15156,"journal":{"name":"Journal of biotechnology & biomaterials","volume":"33 1","pages":"1-4"},"PeriodicalIF":0.0,"publicationDate":"2017-11-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"82170233","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 6

Liposomes for Drug Delivery 用于药物递送的脂质体

Journal of biotechnology & biomaterials Pub Date : 2017-11-06 DOI: 10.4172/2155-952X.1000276

Durgavati Yadav, K. Sandeep, Deepak Pandey, Ranu K. Dutta

引用次数: 149

Quantitative Analysis of AntiHevea brasiliensis Antibody Cross-Reactivity against Taraxacum kok-saghyz Latex Proteins Demonstrates Significantly Reduced Antibody Recognition 抗巴西橡胶树抗体与Taraxacum kok-saghyz乳胶蛋白交叉反应性的定量分析表明抗体识别显著降低

Journal of biotechnology & biomaterials Pub Date : 2017-10-30 DOI: 10.4172/2155-952X.1000270

Julian T. Dafoe, Fang Huang, T. C. Yang

{"title":"Quantitative Analysis of AntiHevea brasiliensis Antibody Cross-Reactivity against Taraxacum kok-saghyz Latex Proteins Demonstrates Significantly Reduced Antibody Recognition","authors":"Julian T. Dafoe, Fang Huang, T. C. Yang","doi":"10.4172/2155-952X.1000270","DOIUrl":"https://doi.org/10.4172/2155-952X.1000270","url":null,"abstract":"The annual plant Taraxacum kok-saghyz, commonly called Russian dandelion, has recently gained commercial attention as an alternative source to the rubber tree, Hevea brasiliensis, for natural rubber latex. While producing rubber of equivalent quality, the potential application of TKS latex for hypoallergenic products remains to be determined. In order to quantify the allergenicity of TKS latex proteins, we compared the extent of anti-Hevea latex polyclonal and monoclonal antibody cross-reactivity toward Hevea latex proteins to that of TKS latex proteins by quantitative ELISA methods and semi-quantitative image densitometry. ELISA measurement of polyclonal antibody recognition toward TKS latex proteins was on the order of one-tenth relative to equal amounts of Hevea latex protein, while recognition by the monoclonal antibody was below 2%. Immunoblots confirmed significantly reduced polyclonal antibody recognition toward TKS latex proteins, in the same range as the cross-reactivity exhibited toward the rubber-producing plant, Lactuca sativa and Glycine max, a non-rubber-producing plant. Despite the presence of cross-reactivity, these quantitative results support TKS as an alternative source of latex that may require lessintensive processing for the reduction of antigenic proteins in the manufacture of hypoallergenic natural rubber latex products.","PeriodicalId":15156,"journal":{"name":"Journal of biotechnology & biomaterials","volume":"278 1","pages":"1-8"},"PeriodicalIF":0.0,"publicationDate":"2017-10-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"80099226","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Update on Fungal Disease: From Establish Infection to Clinical Manifestation 真菌疾病最新进展:从感染到临床表现

Journal of biotechnology & biomaterials Pub Date : 2017-10-25 DOI: 10.4172/2155-952X.1000273

W. Melo, L. Scorzoni, S. A. Rossi, C. B. Costa-Orlandi, Mônica Yonashiro, M. J. Mendes-Giannini, Ana Marisa Fusco Almeida

引用次数: 0

Spray flash evaporation for the continuous production of high performance nano-drugs: New challenges for a new disruptive process 连续生产高性能纳米药物的喷雾闪蒸:颠覆性新工艺的新挑战

Journal of biotechnology & biomaterials Pub Date : 2017-10-20 DOI: 10.4172/2155-952X-C1-075

D. Spitzer

引用次数: 1

Sol-Gel Mediated Synthesis of Pure Hydroxyapatite at Different Temperatures and Silver Substituted Hydroxyapatite for Biomedical Applications 溶胶-凝胶在不同温度下合成纯羟基磷灰石及银取代羟基磷灰石在生物医学上的应用

Journal of biotechnology & biomaterials Pub Date : 2017-10-19 DOI: 10.4172/2155-952X.1000275

Kalaiselvi, R. Mathammal, P. Anitha

引用次数: 5

Hybrid Amniotic Membrane Dressing with Green Silver Nanoparticles as Bioengineered Skin for Wounds and Burns: A Pilot Studies 混合羊膜敷料与绿色纳米银作为生物工程皮肤伤口和烧伤:一个试点研究

Journal of biotechnology & biomaterials Pub Date : 2017-09-28 DOI: 10.4172/2155-952X.1000272

Balasundari Ramesh, Jaikanth Chandrasekaran, S. Jeevankumar, G. Jacob, K. Cherian

{"title":"Hybrid Amniotic Membrane Dressing with Green Silver Nanoparticles as Bioengineered Skin for Wounds and Burns: A Pilot Studies","authors":"Balasundari Ramesh, Jaikanth Chandrasekaran, S. Jeevankumar, G. Jacob, K. Cherian","doi":"10.4172/2155-952X.1000272","DOIUrl":"https://doi.org/10.4172/2155-952X.1000272","url":null,"abstract":"Fabrication of 3D novel scaffolds is very challenging and critical to achieve the appropriate function for tissue regeneration. This study aims to develop a hybrid biological nano-scaffold using Liquid nitrogen (LN2) decellularised human amniotic membrane (hAM), human umbilical cord derived collagen nano-fibres and green silver nanoparticles obtained by green synthesis of curcumin. A novel attempt was made to develop in vitro bioengineered dermal layer by seeding the scaffold with cord blood derived Mesenchymal stem cells (CBMSCs) and trans-differentiating them to keratinocytes and dermal fibroblast like cells using keloid foreskin conditioned media. Furthermore, the small animal experiments in Albino Wistar rats prove it to be a safe healing product without scar formation. This novel scaffold can be a promising dressing material for none healing ulcers and burns. This scaffold has long shelf life, easy to apply, have sustained/controlled release of silver, the minimum frequency of dressing changes, manage excessive exudates, moisture retainment, reduce inflammation and facilitate autolytic debridement.","PeriodicalId":15156,"journal":{"name":"Journal of biotechnology & biomaterials","volume":"16 1","pages":"1-8"},"PeriodicalIF":0.0,"publicationDate":"2017-09-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"88103898","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 7

Green Synthesis of Zinc Oxide Nanoparticles Using Parthenium hysterophorus Leaf Extract and Evaluation of their Antibacterial Properties 子宫草叶提取物绿色合成氧化锌纳米颗粒及其抗菌性能评价

Journal of biotechnology & biomaterials Pub Date : 2017-09-17 DOI: 10.4172/2155-952X.1000271

A. Datta, C. Patra, H. Bharadwaj, Sukhjeet Kaur, N. Dimri, Robinka Khajuria

{"title":"Green Synthesis of Zinc Oxide Nanoparticles Using Parthenium hysterophorus Leaf Extract and Evaluation of their Antibacterial Properties","authors":"A. Datta, C. Patra, H. Bharadwaj, Sukhjeet Kaur, N. Dimri, Robinka Khajuria","doi":"10.4172/2155-952X.1000271","DOIUrl":"https://doi.org/10.4172/2155-952X.1000271","url":null,"abstract":"Parthenium hysterophorus an annual herbaceous weed, is known for its vigorous growth and as a causative agent for contact dermatitis, hay fever and diarrhea in humans and systemic toxicity in livestock. However, in spite of all the problems associated with this weed, it has been reported to have pharmacological activities. The objective of this study is to evaluate the potential of Parthenium hysterophorus leaf extracts for the synthesis of zinc oxide nanoparticles and their antimicrobial properties. Nanoparticles were extracted with the help of aqueous, methanolic and ethanolic solutions of P. hysterophorus leaves. Since a higher yield of nanoparticles was observed when synthesised using aqueous extract therefore these were used in further experiments. Synthesised nanoparticles were characterised using UV-Vis spectroscopy with maximum absorbance peak at 400 nm. SEM and TEM analysis revealed that the particles were spherical and cylindrical in shape with average particle size ranging from 16-45 nm. The chemical group association and elemental composition of nanoparticles was analysed using FTIR and SEM-EDX. Green synthesized zinc oxide nanoparticles were evaluated for antimicrobial activities against Gram positive and Gram negative bacteria. Zinc nanoparticles exhibited maximum zone of inhibition against Enterobacter aerogenes (36 mm) while least activity was seen against Staphylococcus aureus and Bacillus subtilis.","PeriodicalId":15156,"journal":{"name":"Journal of biotechnology & biomaterials","volume":"64 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2017-09-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"85775666","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 74

Isolation and Identification of Mycobacterium tuberculosis with Mixed Growth from Positive MGIT 960 Cultures by Re-Decontamination 从MGIT 960阳性培养物中分离和鉴定混合生长结核分枝杆菌

Journal of biotechnology & biomaterials Pub Date : 2017-09-05 DOI: 10.4172/2155-952X.1000267

V. Ahmad, M. Hanif, K. Chopra, Z. Sidiq, K. Dwivedi, Divy Shrivastsava

引用次数: 3