Balasundari Ramesh, Jaikanth Chandrasekaran, S. Jeevankumar, G. Jacob, K. Cherian
{"title":"混合羊膜敷料与绿色纳米银作为生物工程皮肤伤口和烧伤:一个试点研究","authors":"Balasundari Ramesh, Jaikanth Chandrasekaran, S. Jeevankumar, G. Jacob, K. Cherian","doi":"10.4172/2155-952X.1000272","DOIUrl":null,"url":null,"abstract":"Fabrication of 3D novel scaffolds is very challenging and critical to achieve the appropriate function for tissue regeneration. This study aims to develop a hybrid biological nano-scaffold using Liquid nitrogen (LN2) decellularised human amniotic membrane (hAM), human umbilical cord derived collagen nano-fibres and green silver nanoparticles obtained by green synthesis of curcumin. A novel attempt was made to develop in vitro bioengineered dermal layer by seeding the scaffold with cord blood derived Mesenchymal stem cells (CBMSCs) and trans-differentiating them to keratinocytes and dermal fibroblast like cells using keloid foreskin conditioned media. Furthermore, the small animal experiments in Albino Wistar rats prove it to be a safe healing product without scar formation. This novel scaffold can be a promising dressing material for none healing ulcers and burns. This scaffold has long shelf life, easy to apply, have sustained/controlled release of silver, the minimum frequency of dressing changes, manage excessive exudates, moisture retainment, reduce inflammation and facilitate autolytic debridement.","PeriodicalId":15156,"journal":{"name":"Journal of biotechnology & biomaterials","volume":"16 1","pages":"1-8"},"PeriodicalIF":0.0000,"publicationDate":"2017-09-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"7","resultStr":"{\"title\":\"Hybrid Amniotic Membrane Dressing with Green Silver Nanoparticles as Bioengineered Skin for Wounds and Burns: A Pilot Studies\",\"authors\":\"Balasundari Ramesh, Jaikanth Chandrasekaran, S. Jeevankumar, G. Jacob, K. Cherian\",\"doi\":\"10.4172/2155-952X.1000272\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"Fabrication of 3D novel scaffolds is very challenging and critical to achieve the appropriate function for tissue regeneration. This study aims to develop a hybrid biological nano-scaffold using Liquid nitrogen (LN2) decellularised human amniotic membrane (hAM), human umbilical cord derived collagen nano-fibres and green silver nanoparticles obtained by green synthesis of curcumin. A novel attempt was made to develop in vitro bioengineered dermal layer by seeding the scaffold with cord blood derived Mesenchymal stem cells (CBMSCs) and trans-differentiating them to keratinocytes and dermal fibroblast like cells using keloid foreskin conditioned media. Furthermore, the small animal experiments in Albino Wistar rats prove it to be a safe healing product without scar formation. This novel scaffold can be a promising dressing material for none healing ulcers and burns. This scaffold has long shelf life, easy to apply, have sustained/controlled release of silver, the minimum frequency of dressing changes, manage excessive exudates, moisture retainment, reduce inflammation and facilitate autolytic debridement.\",\"PeriodicalId\":15156,\"journal\":{\"name\":\"Journal of biotechnology & biomaterials\",\"volume\":\"16 1\",\"pages\":\"1-8\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2017-09-28\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"7\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Journal of biotechnology & biomaterials\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.4172/2155-952X.1000272\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of biotechnology & biomaterials","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.4172/2155-952X.1000272","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
Hybrid Amniotic Membrane Dressing with Green Silver Nanoparticles as Bioengineered Skin for Wounds and Burns: A Pilot Studies
Fabrication of 3D novel scaffolds is very challenging and critical to achieve the appropriate function for tissue regeneration. This study aims to develop a hybrid biological nano-scaffold using Liquid nitrogen (LN2) decellularised human amniotic membrane (hAM), human umbilical cord derived collagen nano-fibres and green silver nanoparticles obtained by green synthesis of curcumin. A novel attempt was made to develop in vitro bioengineered dermal layer by seeding the scaffold with cord blood derived Mesenchymal stem cells (CBMSCs) and trans-differentiating them to keratinocytes and dermal fibroblast like cells using keloid foreskin conditioned media. Furthermore, the small animal experiments in Albino Wistar rats prove it to be a safe healing product without scar formation. This novel scaffold can be a promising dressing material for none healing ulcers and burns. This scaffold has long shelf life, easy to apply, have sustained/controlled release of silver, the minimum frequency of dressing changes, manage excessive exudates, moisture retainment, reduce inflammation and facilitate autolytic debridement.