Journal of Biomedical and Clinical Research最新文献_第8页

Hemisection- A Saviour for Periodontally & Cariously Involved Permanent Mandibular Molar 半切开术-治疗牙周及严重受累的恒颌磨牙的救星

Journal of Biomedical and Clinical Research Pub Date : 2021-12-31 DOI: 10.47363/jcbr/2021(3)138

M. Yadav, Shilpi K Pandey, Poonam Agrawa, Devikarani S Khurpade, Ankita Roy, Shubham Bhuktar

引用次数: 0

Study of the Content of Heavy Metals in Foods in Bulgaria 保加利亚食品中重金属含量的研究

Journal of Biomedical and Clinical Research Pub Date : 2021-12-01 DOI: 10.2478/jbcr-2021-0019

Ivelina R. Drambozova

引用次数: 0

Single-Dose Cold Modified Bretschneider Solution for Myocardial Protection in the Surgical Treatment of Patients with Aortic Valve Stenosis and Left Ventricular Hypertrophy 单剂量冷改良Bretschneider溶液在主动脉瓣狭窄和左心室肥厚患者手术治疗中的心肌保护作用

Journal of Biomedical and Clinical Research Pub Date : 2021-12-01 DOI: 10.2478/jbcr-2021-0021

Petar H. Uzov, Chavdar M. Ivanov

引用次数: 0

Carrier Screening for Single-Gene Disorders – A Brief Review 单基因疾病的携带者筛选综述

Journal of Biomedical and Clinical Research Pub Date : 2021-12-01 DOI: 10.2478/jbcr-2021-0015

K. Kovacheva, S. Nikolova, Z. Kamburova

{"title":"Carrier Screening for Single-Gene Disorders – A Brief Review","authors":"K. Kovacheva, S. Nikolova, Z. Kamburova","doi":"10.2478/jbcr-2021-0015","DOIUrl":"https://doi.org/10.2478/jbcr-2021-0015","url":null,"abstract":"Summary Carrier screening (CS) is an approach to pre-reproductive identification of couples at risk of having offspring with a single-gene disorder (SGD). The goal of CS is to facilitate reproductive autonomy and informed decision-making of the revealed risk couples. Initially, CS was introduced in the 1970s among the Ashkenazi Jewish population in the USA with a high incidence of Tay-Sachs disease. Until recently, CS had a limited application, mainly among individuals of a particular ethnicity or family history of SGD, and a limited number of conditions are included. Implementing the revolutionary technology of next-generation sequencing revealed a much more comprehensive range of possibilities for CS. The expanded alternative variant introduced a new concept that allows screening for hundreds to thousands of SGDs without pre-selection of individuals based on their ancestry or family history. Furthermore, the number of detected pathogenic variants is much higher compared to the traditional CS. Currently, there is insufficient experience with the application of expanded CS, and the lack of uniform guidelines is a major problem when it comes to massive implementation. Nevertheless, the significant potential of the expanded CS has given rise to a growing interest in it and provoked in-depth discussions about the opportunities of widespread application in practice.","PeriodicalId":15099,"journal":{"name":"Journal of Biomedical and Clinical Research","volume":"138 1","pages":"105 - 116"},"PeriodicalIF":0.0,"publicationDate":"2021-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"73951167","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A Review of Results from Clinical Trials Based on Co-Variants of the R-CHOP Regimen in Untreated Patients with Diffuse Large B-cell Lymphoma 未经治疗的弥漫性大b细胞淋巴瘤患者R-CHOP方案共变临床试验结果综述

Journal of Biomedical and Clinical Research Pub Date : 2021-12-01 DOI: 10.2478/jbcr-2021-0016

V. S. Popova

{"title":"A Review of Results from Clinical Trials Based on Co-Variants of the R-CHOP Regimen in Untreated Patients with Diffuse Large B-cell Lymphoma","authors":"V. S. Popova","doi":"10.2478/jbcr-2021-0016","DOIUrl":"https://doi.org/10.2478/jbcr-2021-0016","url":null,"abstract":"Summary Since 2000, new drugs for treating lymphoproliferative diseases have been introduced in haematology, and their initial indications have been gradually expanded. Some of the so-called „target molecules“ have shown good efficacy when treating some malignant non-Hodgkin lymphomas. The possibility of achieving therapeutic disease control in some indolent non-Hodgkin lymphomas using drugs with a different mechanism of action from that of cytostatics has logically raised the question of a change in therapeutic management in other lymphoproliferative diseases. Since 2010, clinical trials have been initiated with untreated patients with aggressive non-Hodgkin‘s lymphoma - diffuse large B-cell lymphoma as the primary target. The key aim of most clinical trials has been to compare the efficacy of the so-called „gold standard“ - rituximab, cyclophosphamide, doxorubicin, oncovin, prednisolone (R- CHOP) versus its derivatives. The review discusses the results of completed clinical trials published on the Internet. These trials covered covariants of the R-CHOP regimen used as first-line treatment of patients with diffuse large B-cell lymphoma. The review also includes drugs registered in Bulgaria for the last ten years but with a different clinical indication at present. The results of five clinical trials in which obinotuzumab, venetoclax, lenalidomide, bortezomib, and ibrutinib were used are presented.","PeriodicalId":15099,"journal":{"name":"Journal of Biomedical and Clinical Research","volume":"15 2 1","pages":"117 - 123"},"PeriodicalIF":0.0,"publicationDate":"2021-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"85819699","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Relationship between Elevated Maternal Serum Podocalyxin Concentrations with Blood Pressure Values and Routine Laboratory Parameters in Preeclampsia 子痫前期孕妇血足alyxin浓度升高与血压值及常规实验室参数的关系

Journal of Biomedical and Clinical Research Pub Date : 2021-12-01 DOI: 10.2478/jbcr-2021-0020

N. Popovski, A. Nikolov, Yordan D. Popov, S. Blazheva

{"title":"Relationship between Elevated Maternal Serum Podocalyxin Concentrations with Blood Pressure Values and Routine Laboratory Parameters in Preeclampsia","authors":"N. Popovski, A. Nikolov, Yordan D. Popov, S. Blazheva","doi":"10.2478/jbcr-2021-0020","DOIUrl":"https://doi.org/10.2478/jbcr-2021-0020","url":null,"abstract":"Summary Podocalyxin (PCX) is a glycoprotein member of the CD34 transmembrane sialomucin family and covers the surface of podocytes. It is assumed to be a marker of glomerular endothelial injury. In conditions, podocalyxin excretion in the urine is increased as a result of podocyte injury. Recent studies show that PCX is expressed not only in kidneys but also in the endothelial cells of other organs. Preeclampsia (PE) is characterized by new-onset hypertension, generalized endothelial injury, and glomerular alteration. Having this in mind, in the present study, we aimed to: (1) determine circulating PCX levels in sera of women with preeclampsia and normal pregnancy; (2) explore for an association between PCX and blood pressure and (3) investigate a possible relationship between PCX and routine laboratory markers of cardiac and renal injury/dysfunction. Fifty-five women with preeclampsia were examined. The mean age of patients was 24.9±6 years, and the mean age of the control group of 35 women with normal pregnancies was 24.7±5.4 years. The enzyme-linked immunosorbent assay (ELISA) was used to determine concentrations of PCX. Levels of serum PCX in preeclamptic women were statistically significantly higher than those in women with normal pregnancy: 2.66±0.67 vs. 2.40±0.33 ng/ml (p=0.03). Podocalyxin correlated with SBP (r=0.30; p=0.004), DBP (r=0.35; p=0.0007), uric acid (r=0.32; p=0.002), CPK (r=0.22; 0.03) and its isoenzyme CK-MB (r=0.21; p=0.04). Our results showed significantly higher levels of serum PCX in women with preeclampsia than in healthy pregnancy. Elevated podocalyxin levels are associated with an increase in blood pressure in preeclampsia. We found a relationship between PCX and routine laboratory indicators of cardiac (CPK and CK-MB) and renal injury/dysfunction (uric acid). PCX has the potential as a future preeclampsia diagnostic biomarker.","PeriodicalId":15099,"journal":{"name":"Journal of Biomedical and Clinical Research","volume":"27 1","pages":"156 - 161"},"PeriodicalIF":0.0,"publicationDate":"2021-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"73923765","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Review on Anastomotic Leak Rate after ICG Angiography during Minimally Invasive Colorectal Surgery 微创结直肠手术中ICG造影吻合口漏率的研究进展

Journal of Biomedical and Clinical Research Pub Date : 2021-12-01 DOI: 10.2478/jbcr-2021-0017

T. Yotsov, M. Karamanliev, S. Maslyankov, D. Dimitrov

{"title":"Review on Anastomotic Leak Rate after ICG Angiography during Minimally Invasive Colorectal Surgery","authors":"T. Yotsov, M. Karamanliev, S. Maslyankov, D. Dimitrov","doi":"10.2478/jbcr-2021-0017","DOIUrl":"https://doi.org/10.2478/jbcr-2021-0017","url":null,"abstract":"Summary Colorectal cancer is the 3rd most common type of cancer worldwide. The most devastating complication after colorectal surgery remains the anastomotic leak (AL). Many techniques have been developed to reduce its rate. One such new method is perfusion angiography using indocyanine green (ICG). A literary search in PUBMED on 1.03.2021 for full-text English articles published between 2014 and 2021 was performed. ICG, colorectal cancer, and angiography were the keywords we used. The review was performed following the recommendations of the Preferred Reporting Items for Systematic Reviews and Meta-Analyses guidelines. The literature search yielded 27 results when searching the database of PUBMED with the above keywords. Twenty-one out of 27 identified articles were included. Six were excluded from the analysis – four case reports, one review on the evolution of treating gastrointestinal cancers, and one containing no information on AL rate with ICG. One included article was RCT, sixteen were cohort studies, and four were meta-analyses or reviews. All articles reported a reduction in the anastomotic leak rate. However, the reduction was significant only in nine of them. Anastomotic leak is a severe complication and a subject of extensive research. Perfusion angiography with ICG is a step towards predicting and preventing AL, although it does not guarantee success in all cases.","PeriodicalId":15099,"journal":{"name":"Journal of Biomedical and Clinical Research","volume":"82 1","pages":"124 - 130"},"PeriodicalIF":0.0,"publicationDate":"2021-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"77272631","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Effect of Aminosteroid U74389G in a Model of Inflammatory Bowel Disease in Rats 氨基类固醇U74389G在炎症性肠病大鼠模型中的作用

Journal of Biomedical and Clinical Research Pub Date : 2021-12-01 DOI: 10.2478/jbcr-2021-0018

P. Krastev, A. Blazhev, G. Stavreva

{"title":"Effect of Aminosteroid U74389G in a Model of Inflammatory Bowel Disease in Rats","authors":"P. Krastev, A. Blazhev, G. Stavreva","doi":"10.2478/jbcr-2021-0018","DOIUrl":"https://doi.org/10.2478/jbcr-2021-0018","url":null,"abstract":"Summary Lazaroid U-74389G is a synthetic 21-aminosteroid with free radical-scavenging and anti-inflammatory effects. This study was designed to evaluate the anti-inflammatory activity of U-74389G on experimental 2,4-dinitrobenzene sulfonic acid hydrate (DNBS)-induced colitis in Wistar rats. Five experimental groups were formed: a sham control group, a control group, treated with 0.25 ml of 50% ethanol intrarectally (n=8), a group treated with DNBS (30 mg in 0.25 ml of 50% ethanol administered intrarectally, (n=8), a group treated with DNBS and U-74389G at a daily dose of 15 mg/kg i.p. (n=8), and a group treated with DNBS and sulfasalazine, orally, at a dose of 300 mg/kg. During the experiment, the bodyweight of the rats, food intake, stool consistency, and presence of blood in the stool were recorded as markers of clinical condition. On day 6, colonic tissues were excised and scored for macroscopic and histological damage. Blood samples were taken to measure levels of cytokines by ELISA methods. DNBS decreased significantly body weight (from 237.00±2.52 g to 212.50±6.25 g, p=0.04). The rats treated with U-74389G showed greater food intake and weight gain. U-74389G reduced ulceration index: the U-74389G score was 1.25±0.25, and the DNBS score –3.87±0.61; p<0.05. All other macroscopic parametersassessed were significantly improved in rats treated with U-74389G. The levels of inflammatory cytokines IL-1, IL-6, and TNF-α, were significantly lower than those of the DNBS group, while U-74389G significantly elevated the level of anti-inflammatory IL-10. These findings indicate that U-74389G significantly inhibits colonic inflammatory damages in a rat model of inflammatory bowel disease.","PeriodicalId":15099,"journal":{"name":"Journal of Biomedical and Clinical Research","volume":"27 1","pages":"131 - 139"},"PeriodicalIF":0.0,"publicationDate":"2021-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"87692205","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Molecular Surveillance and Assessment of Ceftolozane/Tazobactam Resistance with Common β-Lactam Antibiotics and β-Lactamase Genes 常用β-内酰胺类抗生素及β-内酰胺酶基因对头孢唑烷/他唑巴坦耐药性的分子监测与评价

Journal of Biomedical and Clinical Research Pub Date : 2021-09-30 DOI: 10.47363/jcbr/2021(3)136

Jourjina Subih Alkhouri, F. Santiago, Candace Guzman-Cole, Sona Garsevanyan, Suzanne S. Sindi, M. Barlow

{"title":"Molecular Surveillance and Assessment of Ceftolozane/Tazobactam Resistance with Common β-Lactam Antibiotics and β-Lactamase Genes","authors":"Jourjina Subih Alkhouri, F. Santiago, Candace Guzman-Cole, Sona Garsevanyan, Suzanne S. Sindi, M. Barlow","doi":"10.47363/jcbr/2021(3)136","DOIUrl":"https://doi.org/10.47363/jcbr/2021(3)136","url":null,"abstract":"Ceftolozane/tazobactam (c/t) is a potent β-lactam antibiotic which combines the fifth generation cephalosporin ceftolozane and tazobactam, a β-lactamase inhibitor. The c/t combination therapy was approved in 2014 for the treatment of multidrug resistant (MDR) Enterobacteriaceae, especially intra-abdominal and urinary tract infections. The aim of this study is to assess c/t activity and to examine the association of c/t resistance with four common β-lactamase resistance genes found in clinical Enterobacteriaceae isolates collected from mainly urinary tract infections in an agricultural region in California (USA) between 2013-2020. We tested 993 Extended Spectrum β-lactamases (ESBL) producing Enterobacteriaceae isolates (885 E. coli, 94 K. pneumoniae, 14 other) for c/t susceptibility by Kirby-Bauer disk diffusion and screened using PCR for four common resistance genes with β-lactamase activity(blaTEM, blaOXA, blaSHV, and blaCTX-M) for 855 of the isolates. We also investigated co-resistance of c/t and nine other β-lactam antibiotics. We found that most isolates were susceptible to c/t (58.3%), while 38.5% showed intermediate resistance, and 3.2% were resistant. We also found that K. pneumoniae isolates were more resistant to c/t than E. coli isolates, and that c/t may be a good alternative to carbapenems, in that that some carbapenem resistant isolates were susceptible to c/t. Genotypic analysis showed blaSHV and blaCTX-M are independently associated with elevated c/t resistance. Although c/t demonstrated strong activity against Enterobacteriaceae, the high percentage of isolates with intermediate susceptibility emphasizes the need for close monitoring and continued surveillance for c/t resistance among ESBLs","PeriodicalId":15099,"journal":{"name":"Journal of Biomedical and Clinical Research","volume":"46 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"72859006","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Relative Concentrations of Amino Acids in the Exoskeleton of Male and Female Neopetrolisthes Maculatus 雄性和雌性斑点新石鱼外骨骼中氨基酸的相对浓度

Journal of Biomedical and Clinical Research Pub Date : 2021-09-30 DOI: 10.47363/jcbr/2021(3)133

Adeyeye Ei, Idowu Ot, Akinsola Af, Olatunya Am, Popoola Ok, A. O, Gbolagade Ay

{"title":"Relative Concentrations of Amino Acids in the Exoskeleton of Male and Female Neopetrolisthes Maculatus","authors":"Adeyeye Ei, Idowu Ot, Akinsola Af, Olatunya Am, Popoola Ok, A. O, Gbolagade Ay","doi":"10.47363/jcbr/2021(3)133","DOIUrl":"https://doi.org/10.47363/jcbr/2021(3)133","url":null,"abstract":"Amino acid levels in the exoskeleton of the heterosexual samples of Neopetrolisthes maculatus were evaluated. Twenty parameters were reported on; 13 of these values were greater in the female than the male (i.e. 13/20 or 65.0%). Values in female > male were observed in Val, Thr, Lys, Met, Phe, Trp, Gly, Ala, Asp, Arg, Cys, total amino acids and protein. The variations between the male and female amino acid values were low having coefficient of variation percent range of 0.0579-16.9. The amino acid levels were significantly different between the heterosexuals at r=0.01. Limiting amino acids on the various scoring standards were egg (Cys=0.3172 and 0.3511), provisional essential amino acid scoring pattern (Lys = 0.4145 and 0.4746), preschool child requirements (Lys =0.3930 and 0.4501) and all the three scores were significantly different at r=0.01. The EAAI1 (compared to soybean) were 1.18 (male) and 1.16 (female), EAAI2 (compared to egg) were 88.5 (male) and 88.3 (female); their corresponding BV were 84.7 and 84.5. P-PER1 range was 1.86 (male) - 1.24 (female) and P-PER2 range was 2.02 (male) - 1.41 (female). Total EAA was 37.7g/100g protein (male) and 37.0g/100g protein (female). In the estimates of amino acid requirements of school boys at ages 10-12years (mg/kg/day), male exoskeleton would provide more than the requirement in Phe + Tyr (186%), Trp (25.8%) and Val (48.1%) whereas in female the following were higher than the standards: Phe + Tyr (218%), Thr (8.86%), Trp (35.0%), Val (61.6%) and TEAAs (4.01%)","PeriodicalId":15099,"journal":{"name":"Journal of Biomedical and Clinical Research","volume":"2674 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"74810329","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0