Journal of Bioenergetics and Biomembranes最新文献_第9页

The status and trends of mitochondrial dynamics research: A global bibliometric and visualized analysis. 线粒体动力学研究的现状和趋势:全球文献计量和可视化分析。

IF 3 4区生物学

Journal of Bioenergetics and Biomembranes Pub Date : 2023-02-01 DOI: 10.1007/s10863-023-09959-6

Zijian Guo, Zehua Wang, Zhenzhong Gao, Tengda Feng, Yingjie Gao, Zhiwen Yin, Zui Tian, Yang Liu, Xingjia Mao, Chuan Xiang

{"title":"The status and trends of mitochondrial dynamics research: A global bibliometric and visualized analysis.","authors":"Zijian Guo, Zehua Wang, Zhenzhong Gao, Tengda Feng, Yingjie Gao, Zhiwen Yin, Zui Tian, Yang Liu, Xingjia Mao, Chuan Xiang","doi":"10.1007/s10863-023-09959-6","DOIUrl":"https://doi.org/10.1007/s10863-023-09959-6","url":null,"abstract":"Background: Mitochondria are remarkably dynamic organelles encapsulated by bilayer membranes. The dynamic properties of mitochondria are critical for energy production.Aims: The aim of our study is to investigate the global status and trends of mitochondrial dynamics research and predict popular topics and directions in the field.Methods: Publications related to the studies of mitochondrial dynamics from 2002 to 2021 were retrieved from Web of Science database. A total of 4,576 publications were included. Bibliometric analysis was conducted by visualization of similarities viewer and GraphPadPrism 5 software.Results: There is an increasing trend of mitochondrial dynamics research during the last 20 years. The cumulative number of publications about mitochondrial dynamics research followed the logistic growth model [Formula: see text]. The USA made the highest contributions to the global research. The journal Biochimica et Biophysica Acta (BBA)-Molecular Cell Research had the largest publication numbers. Case Western Reserve University is the most contributive institution. The main research orientation and funding agency were cell biology and HHS. All keywords related studies could be divided into three clusters: \"Related disease research\", \"Mechanism research\" and \"Cell metabolism research\".Conclusions: Attention should be drawn to the latest popular research and more efforts will be put into mechanistic research, which may inspire new clinical treatments for the associated diseases.","PeriodicalId":15080,"journal":{"name":"Journal of Bioenergetics and Biomembranes","volume":null,"pages":null},"PeriodicalIF":3.0,"publicationDate":"2023-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9942064/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9305209","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Correction to: Glycolytic inhibitors 2-deoxyglucose and 3-bromopyruvate synergize with photodynamic therapy respectively to inhibit cell migration. 修正:糖酵解抑制剂2-脱氧葡萄糖和3-溴丙酮酸分别与光动力疗法协同作用，抑制细胞迁移。

IF 3 4区生物学

Journal of Bioenergetics and Biomembranes Pub Date : 2023-02-01 DOI: 10.1007/s10863-022-09955-2

Xiaolan Feng, Pan Wang, Quanhong Liu, Ting Zhang, Bingjie Mai, Xiaobing Wang

引用次数: 0

Resveratrol ameliorates myocardial ischemia/reperfusion induced necroptosis through inhibition of the Hippo pathway. 白藜芦醇通过抑制Hippo通路改善心肌缺血/再灌注诱导的坏死下垂。

IF 3 4区生物学

Journal of Bioenergetics and Biomembranes Pub Date : 2023-02-01 DOI: 10.1007/s10863-022-09954-3

Hao Tian, Yonghong Xiong, Zhongyuan Xia

{"title":"Resveratrol ameliorates myocardial ischemia/reperfusion induced necroptosis through inhibition of the Hippo pathway.","authors":"Hao Tian, Yonghong Xiong, Zhongyuan Xia","doi":"10.1007/s10863-022-09954-3","DOIUrl":"https://doi.org/10.1007/s10863-022-09954-3","url":null,"abstract":"Myocardial ischemia-reperfusion (I/R) injury is a major cause of poor hemodynamic reconstitution outcomes after myocardial infarction or circulatory arrest. Currently, the search for effective therapeutic agents and tools is a focus of research in the field of myocardial I/R injury. Resveratrol (Res) has been extensively studied in recent years because of its good cardiovascular therapeutic effects, but its specific mechanism of action has not been fully elucidated. Therefore, the aim of this study was to investigate the mechanism of interaction between myocardial I/R injury and Res in vitro and in vivo. In our in vivo study, we used PI/TUNEL staining and western blotting to detect relevant necroptotic key molecules such as RIP1, RIP3 and p-MLKL/MLKL to observe myocardial necroptosis. The extent of myocardial injury was determined using hematoxylin and eosin (HE) staining and 2,3,5-triphenyltetrazolium chloride (TTC) staining as well as serum levels of CK-MB and LDH and echocardiography. In the in vitro study, cellular injury was assessed by CCK-8 and cell supernatant LDH levels. In addition, we used small interfering RNA (siRNA) transfection to knock down YAP, a key effector molecule of the Hippo pathway, to validate the molecular mechanism of action by which Res exerts myocardial protection. The localization of YAP in H9c2 cardiomyocytes was examined using immunofluorescence. Our data demonstrated that Res could ameliorate myocardial I/R-induced necroptosis by modulating the Hippo pathway, and that the beneficial effect of Res might be associated with nuclear translocation of the transcriptional regulator YAP.","PeriodicalId":15080,"journal":{"name":"Journal of Bioenergetics and Biomembranes","volume":null,"pages":null},"PeriodicalIF":3.0,"publicationDate":"2023-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9255740","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

The evolution of the human mitochondrial bc1 complex- adaptation for reduced rate of superoxide production? 人类线粒体bc1复合体的进化——对超氧化物生成速率降低的适应?

IF 3 4区生物学

Journal of Bioenergetics and Biomembranes Pub Date : 2023-02-01 DOI: 10.1007/s10863-023-09957-8

Hagai Rottenberg

{"title":"The evolution of the human mitochondrial bc1 complex- adaptation for reduced rate of superoxide production?","authors":"Hagai Rottenberg","doi":"10.1007/s10863-023-09957-8","DOIUrl":"https://doi.org/10.1007/s10863-023-09957-8","url":null,"abstract":"The mitochondrial bc1 complex is a major source of mitochondrial superoxide. While bc1-generated superoxide plays a beneficial signaling role, excess production of superoxide lead to aging and degenerative diseases. The catalytic core of bc1 comprises three peptides -cytochrome b, Fe-S protein, and cytochrome c1. All three core peptides exhibit accelerated evolution in anthropoid primates. It has been suggested that the evolution of cytochrome b in anthropoids was driven by a pressure to reduce the production of superoxide. In humans, the bc1 core peptides exhibit anthropoid-specific substitutions that are clustered near functionally critical sites that may affect the production of superoxide. Here we compare the high-resolution structures of bovine, mouse, sheep and human bc1 to identify structural changes that are associated with human-specific substitutions. Several cytochrome b substitutions in humans alter its interactions with other subunits. Most significantly, there is a cluster of seven substitutions, in cytochrome b, the Fe-S protein, and cytochrome c1 that affect the interactions between these proteins at the tether arm of the Fe-S protein and may alter the rate of ubiquinone oxidation and the rate of superoxide production. Another cluster of substitutions near heme bH and the ubiquinone reduction site, Qi, may affect the rate of ubiquinone reduction and thus alter the rate of superoxide production. These results are compatible with the hypothesis that cytochrome b in humans (and other anthropoid primates) evolve to reduce the rate of production of superoxide thus enabling the exceptional longevity and exceptional cognitive ability of humans.","PeriodicalId":15080,"journal":{"name":"Journal of Bioenergetics and Biomembranes","volume":null,"pages":null},"PeriodicalIF":3.0,"publicationDate":"2023-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10842675","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Exposure of combination of environmental pollutant, lead (Pb) and β-amyloid peptides causes mitochondrial dysfunction and oxidative stress in human neuronal cells. 环境污染物、铅和β-淀粉样肽联合暴露可引起人神经元细胞线粒体功能障碍和氧化应激。

IF 3 4区生物学

Journal of Bioenergetics and Biomembranes Pub Date : 2023-02-01 DOI: 10.1007/s10863-023-09956-9

Lakshmi Jaya Madhuri Bandaru, Lokesh Murumulla, Bindu Lasya C, Krishna Prasad D, Suresh Challa

{"title":"Exposure of combination of environmental pollutant, lead (Pb) and β-amyloid peptides causes mitochondrial dysfunction and oxidative stress in human neuronal cells.","authors":"Lakshmi Jaya Madhuri Bandaru, Lokesh Murumulla, Bindu Lasya C, Krishna Prasad D, Suresh Challa","doi":"10.1007/s10863-023-09956-9","DOIUrl":"https://doi.org/10.1007/s10863-023-09956-9","url":null,"abstract":"Exposure to the environmental pollutant lead (Pb) has been linked to Alzheimer's disease (AD), in which mitochondrial dysfunction is a pathological consequence of neuronal degeneration. The toxicity of Pb in combination with β-amyloid peptides (1-40) and (25-35) causes selective death in neuronal cells. However, the precise mechanism through which Pb induces Alzheimer's disease, particularly mitochondrial damage, is unknown. Changes in mitochondrial mass, membrane potential, mitochondrial complex activities, mitochondrial DNA and oxidative stress were examined in neuronal cells of human origin exposed to Pb and β-amyloid peptides (1-40) and (25-35) individually and in different combinations. The results showed depolarization of mitochondrial membrane potential, decrease in mitochondrial mass, ATP levels and mtDNA copy number in Pb and β-amyloid peptides (1-40) and (25-35) exposed cells. Also, significant reductions in the expression of mitochondrial electron transport chain (ETC) complex proteins (ATP5A, COXIV, UQCRC2, SDHB, NDUFS3), as well as down regulation of ETC complex gene expressions such as COXIV, ATP5F1 and NDUFS3 and antioxidant gene expressions like MnSOD and Gpx4 were observed in exposed cells. Furthermore, Pb and β-amyloid peptides exposure resulted in elevated mitochondrial malondialdehyde levels and a decrease in mitochondrial GSH levels. Our findings suggest that Pb toxicity could be one of the causative factors for the mitochondrial dysfunction and oxidative stress in Alzheimer's disease progression.","PeriodicalId":15080,"journal":{"name":"Journal of Bioenergetics and Biomembranes","volume":null,"pages":null},"PeriodicalIF":3.0,"publicationDate":"2023-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9250477","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 4

Separation and analysis of Bacillus subtilis respiratory chain complexes. 枯草芽孢杆菌呼吸链复合物的分离与分析。

IF 3 4区生物学

Journal of Bioenergetics and Biomembranes Pub Date : 2022-12-01 Epub Date: 2022-11-23 DOI: 10.1007/s10863-022-09951-6

Gerardo Ignacio Picón Garrido, Ana Paula García García, Luis González de la Vara, Alicia Chagolla-López, Carlos Gómez-Lojero, Emma Berta Gutiérrez-Cirlos

{"title":"Separation and analysis of Bacillus subtilis respiratory chain complexes.","authors":"Gerardo Ignacio Picón Garrido, Ana Paula García García, Luis González de la Vara, Alicia Chagolla-López, Carlos Gómez-Lojero, Emma Berta Gutiérrez-Cirlos","doi":"10.1007/s10863-022-09951-6","DOIUrl":"https://doi.org/10.1007/s10863-022-09951-6","url":null,"abstract":"Bacillus subtilis is a Gram-positive bacterium with a respiratory chain embedded in the cytoplasmic membrane. The respiratory chain is bifurcated after menaquinol into a cytochrome b6c + caa3 branch and a branch with up to three quinol oxidases. The complexes that generate the proton gradient are b6c, associated with caa3 and aa3 oxidase. The b6c and caa3 complexes form a supercomplex, and it is proposed to form respiratory strings in the membrane. There is still information missing about the quinol branch and if the primary oxidase quinol aa3 is associated with the electron donor complexes. It is unclear whether succinate quinone reductase (SQR) can form associations with the quinol branch or the cytochrome branch. In this paper, we show the separation of an almost pure b6c complex associated with cytochromes c550 and c551. We obtained a b6c + caa3 supercomplex of 600 kDa and SQR, aa3, and NADH dehydrogenase by dodecyl maltoside solubilization and separation of the respiratory chain components by ionic exchange chromatography. We found that aa3 does not associate with other complexes. SQR was associated with the b6c complex in a mutant lacking aa3. This association could facilitate electron transfer from SQR to menaquinone-7. The lack of associations between the abundant quinol oxidase aa3 and other complexes is a feature we cannot explain yet.","PeriodicalId":15080,"journal":{"name":"Journal of Bioenergetics and Biomembranes","volume":null,"pages":null},"PeriodicalIF":3.0,"publicationDate":"2022-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40493561","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 3

Isolation and characterization of a main porin from the outer membrane of Salinibacter ruber. 橡胶盐杆菌外膜主要孔蛋白的分离与表征。

IF 3 4区生物学

Journal of Bioenergetics and Biomembranes Pub Date : 2022-12-01 Epub Date: 2022-10-13 DOI: 10.1007/s10863-022-09950-7

Domenica Farci, Emma Cocco, Marta Tanas, Joanna Kirkpatrick, Andrea Maxia, Elena Tamburini, Wolfgang P Schröder, Dario Piano

{"title":"Isolation and characterization of a main porin from the outer membrane of Salinibacter ruber.","authors":"Domenica Farci, Emma Cocco, Marta Tanas, Joanna Kirkpatrick, Andrea Maxia, Elena Tamburini, Wolfgang P Schröder, Dario Piano","doi":"10.1007/s10863-022-09950-7","DOIUrl":"https://doi.org/10.1007/s10863-022-09950-7","url":null,"abstract":"Salinibacter ruber is an extremophilic bacterium able to grow in high-salts environments, such as saltern crystallizer ponds. This halophilic bacterium is red-pigmented due to the production of several carotenoids and their derivatives. Two of these pigment molecules, salinixanthin and retinal, are reported to be essential cofactors of the xanthorhodopsin, a light-driven proton pump unique to this bacterium. Here, we isolate and characterize an outer membrane porin-like protein that retains salinixanthin. The characterization by mass spectrometry identified an unknown protein whose structure, predicted by AlphaFold, consists of a 8 strands beta-barrel transmembrane organization typical of porins. The protein is found to be part of a functional network clearly involved in the outer membrane trafficking. Cryo-EM micrographs showed the shape and dimensions of a particle comparable with the ones of the predicted structure. Functional implications, with respect to the high representativity of this protein in the outer membrane fraction, are discussed considering its possible role in primary functions such as the nutrients uptake and the homeostatic balance. Finally, also a possible involvement in balancing the charge perturbation associated with the xanthorhodopsin and ATP synthase activities is considered.","PeriodicalId":15080,"journal":{"name":"Journal of Bioenergetics and Biomembranes","volume":null,"pages":null},"PeriodicalIF":3.0,"publicationDate":"2022-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9701654/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"33506361","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Synthesis, biological evaluation and molecular modeling studies of novel 1,2,3-triazole-linked menadione-furan derivatives as P2X7 inhibitors. 新型P2X7抑制剂1,2,3-三唑-甲萘醌-呋喃衍生物的合成、生物学评价及分子模型研究。

IF 3 4区生物学

Journal of Bioenergetics and Biomembranes Pub Date : 2022-12-01 Epub Date: 2022-09-07 DOI: 10.1007/s10863-022-09947-2

Juliana P S Dos Santos, Ruan Carlos B Ribeiro, Juliana V Faria, Murilo L Bello, Carolina G S Lima, Fernanda P Pauli, Amanda A Borges, David R Rocha, Matheus G Moraes, Luana S M Forezi, Vitor F Ferreira, Robson X Faria, Fernando de C da Silva

{"title":"Synthesis, biological evaluation and molecular modeling studies of novel 1,2,3-triazole-linked menadione-furan derivatives as P2X7 inhibitors.","authors":"Juliana P S Dos Santos, Ruan Carlos B Ribeiro, Juliana V Faria, Murilo L Bello, Carolina G S Lima, Fernanda P Pauli, Amanda A Borges, David R Rocha, Matheus G Moraes, Luana S M Forezi, Vitor F Ferreira, Robson X Faria, Fernando de C da Silva","doi":"10.1007/s10863-022-09947-2","DOIUrl":"https://doi.org/10.1007/s10863-022-09947-2","url":null,"abstract":"The P2X7 receptor (P2X7R) is an ion channel that promotes the passage of ions through the membrane through brief stimulation once activated by ATP, its endogenous opener. However, prolonged stimulation with ATP, which occurs in pathological processes, opens a nonselective pore in the plasma membrane, allowing the passage of large molecules and leading to cytokine release or even cell death. In this sense, the search for new inhibitors for this receptor has attracted a great deal of attention in recent years. Considering the booming of biomass upgrading reactions in recent years and the continued efforts to synthesize biologically active molecules containing the 1,2,3-triazole ring, in the present work, we aimed to investigate whether triazole-linked menadione-furan derivatives could present P2X7R inhibitory activity. The novel compounds were tested for their inhibitory activity on ATP-induced dye uptake in peritoneal macrophages. Some have shown promising results, having displayed IC50 values lower than that of the P2X7R inhibitor BBG. Molecular docking studies also indicated that the active compounds bind to an allosteric site on P2X7R, presenting potential P2X7R inhibition.","PeriodicalId":15080,"journal":{"name":"Journal of Bioenergetics and Biomembranes","volume":null,"pages":null},"PeriodicalIF":3.0,"publicationDate":"2022-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40354416","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 6

Effect of H₂O₂ on Na,K-ATPase. H2O2对Na、k - atp酶的影响。

IF 3 4区生物学

Journal of Bioenergetics and Biomembranes Pub Date : 2022-12-01 Epub Date: 2022-11-04 DOI: 10.1007/s10863-022-09948-1

Gvantsa Chkadua, Eka Nozadze, Leila Tsakadze, Lia Shioshvili, Nana Arutinova, Marine Leladze, Sopio Dzneladze, Maia Javakhishvili

{"title":"Effect of H2O2 on Na,K-ATPase.","authors":"Gvantsa Chkadua, Eka Nozadze, Leila Tsakadze, Lia Shioshvili, Nana Arutinova, Marine Leladze, Sopio Dzneladze, Maia Javakhishvili","doi":"10.1007/s10863-022-09948-1","DOIUrl":"https://doi.org/10.1007/s10863-022-09948-1","url":null,"abstract":"Na,K-ATPase is a member of the P-type ATPase family, which transforms the energy of ATP to the transmembrane Na/K gradient that is used to create membrane potential, support the excitability of neurons and myocytes, control pH, and transport substances. The regulation of the Na,K-ATPase function by physiological regulators also comprises a central role in the adaptation of organisms to different conditions. H2O2 is one of the main signaling molecules in redox metabolism and plays important function in cellular physiology. H2O2 also regulates signaling pathways via the specific oxidation of proteins harboring redox-sensitive moieties, like metal centers or cysteine residues, which control their activity. The Na,K-ATPase is redox-sensitive with an \"optimal redox potential range,\" where the reactive oxygen species (ROS), levels beyond this \"optimal range\" are responsible for enzyme inhibition. Thus reactive oxygen species manifest a hermetic effect, which is expressed by biphasic action; stimulation by low doses and inhibition by high doses. This study was aimed to reveal redox-sensitivity of brain synaptic membrane fractions Na,K-ATPase via H2O2 effects. Different concentrations of H2O2 change the kinetic parameters of the enzyme system for MgATP complex, Na+, and K+ differently. Moreover, H2O2 changes p-chloromercuribenzoic acids (PCMB) affinity. H2O2 targets thiols of the Na,K-ATPase - low and high concentrations of H2O2 change the oxidative state of thiolate (S-) from Cys differently, resulting in the corresponding activation or inhibition of the enzyme. Targeting thiols of the Na,K-ATPase tunes the activity of the Na,K-ATPase to the cellular demands and sustains the enzyme activity at the \"optimal\" level.","PeriodicalId":15080,"journal":{"name":"Journal of Bioenergetics and Biomembranes","volume":null,"pages":null},"PeriodicalIF":3.0,"publicationDate":"2022-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40665901","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 4

NDUFA4 promotes cell proliferation by enhancing oxidative phosphorylation in pancreatic adenocarcinoma. NDUFA4 通过增强胰腺腺癌的氧化磷酸化促进细胞增殖。

IF 3 4区生物学

Journal of Bioenergetics and Biomembranes Pub Date : 2022-12-01 Epub Date: 2022-10-29 DOI: 10.1007/s10863-022-09949-0

Yue Zhang, Mengchen Ge, Yuxiang Chen, Yan Yang, Weibo Chen, Di Wu, Huihua Cai, Xuemin Chen, Xinquan Wu

{"title":"NDUFA4 promotes cell proliferation by enhancing oxidative phosphorylation in pancreatic adenocarcinoma.","authors":"Yue Zhang, Mengchen Ge, Yuxiang Chen, Yan Yang, Weibo Chen, Di Wu, Huihua Cai, Xuemin Chen, Xinquan Wu","doi":"10.1007/s10863-022-09949-0","DOIUrl":"10.1007/s10863-022-09949-0","url":null,"abstract":"Pancreatic adenocarcinoma (PAAD) is the third leading cause of cancer-related deaths, with a 5-year relative survival rate of 6%. Hence, novel therapeutic targets need to be urgently explored for PAAD. Recently, oxidative phosphorylation (OXPHOS) has been identified to contribute to the development of PAAD. Nicotinamide adenine dinucleotide + hydrogen (NADH) dehydrogenase (ubiquinone) 1 alpha subcomplex 4 (NDUFA4) is known to affect the mitochondrial respiration pathway. However, the function of NDUFA4 in PAAD remains unclear. In this study, NDUFA4 expression was examined in samples from patients with PAAD using real-time polymerase chain reaction and immunohistochemical staining. Furthermore, cell proliferation and cell cycle were analyzed using Cell Counting Kit-8 assay and flow cytometry. A xenograft tumor model derived from a PAAD cell line was developed to validate the in vitro findings. NDUFA4 was observed to be upregulated in the PAAD samples, and high levels were associated with a poor survival rate. NDUFA4 knockdown reduced cell proliferation by inducing G1 arrest in SW1990 cells. Mechanistically, NDUFA4 knockdown decreased the oxygen consumption rate, cellular adenosine triphosphate level, mitochondrial complex IV activity, and protein levels of COX6C and COX5B, which indicated the suppression of OXPHOS. In contrast, NDUFA4 overexpression exerted the opposite effects. Finally, NDUFA4 knockdown significantly inhibited the growth of the xenograft tumor derived from the SW1990 cell line in vivo. Therefore, NDUFA4 contributes to PAAD proliferation by enhancing OXPHOS.","PeriodicalId":15080,"journal":{"name":"Journal of Bioenergetics and Biomembranes","volume":null,"pages":null},"PeriodicalIF":3.0,"publicationDate":"2022-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40654320","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2