Journal of capillary electrophoresis and microchip technology最新文献_第4页

Fast method development and rapid analysis using a screening approach for enantiomeric separations in capillary electrophoresis. 毛细管电泳中对映体分离的筛选方法的快速开发和快速分析。

Journal of capillary electrophoresis and microchip technology Pub Date : 2003-09-01

M Ilias Jimidar, Willy Van Ael, Rekha Shah, Dirk Redlich, Maurits De Smet

{"title":"Fast method development and rapid analysis using a screening approach for enantiomeric separations in capillary electrophoresis.","authors":"M Ilias Jimidar, Willy Van Ael, Rekha Shah, Dirk Redlich, Maurits De Smet","doi":"","DOIUrl":"","url":null,"abstract":"In order to speed up the trial-and-error process during enantioselective capillary electrophoresis methods development, a systemized approach is proposed to develop methods by applying several screening methods in the search for an initial separation. Screening methods combine high selectivity with broad applicability and are applied to find an initial enantiomeric separation during early pharmaceutical development (pre-Phase 1 to Phase 1). The goal is to achieve enantiomeric separation rapidly in order to characterize the chiral purity of pharmaceutical products. Dedicated, highly efficient screening methods are suggested for basic, neutral, and acidic compounds. In these screening methods, multiple chiral selectors are applied in mixtures at different buffer pH values. For the compounds studied, the technique allows fast method development. Furthermore, it is potentially applicable to a wide range of low-molecular-weight compounds and permits rapid analysis at low cost, since runs are performed in inexpensive, bare silica capillaries using ordinary buffer systems with only typical cyclodextrins as the selector. Along with simplicity and robustness, the approach results in sufficient efficacy (i.e., it is easy, straightforward, and reproducible, with a high success rate). Typical pharmaceutical applications are described. The major advantage of the screening approach to methods development is the decrease in development cycle time. The total screening time for one compound was about 5.3 hr on one CE instrument.","PeriodicalId":15060,"journal":{"name":"Journal of capillary electrophoresis and microchip technology","volume":"8 5-6","pages":"101-10"},"PeriodicalIF":0.0,"publicationDate":"2003-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"24684089","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Critical comparison of extraction procedures for the capillary electrophoretic analysis of opiates in hair. 毛发中阿片类药物毛细管电泳分析提取方法的关键比较。

Journal of capillary electrophoresis and microchip technology Pub Date : 2003-09-01

Elizabete C de Lima, Clóvis L da Silva, Magnólia L N Gauchée, Marina F M Tavares

{"title":"Critical comparison of extraction procedures for the capillary electrophoretic analysis of opiates in hair.","authors":"Elizabete C de Lima, Clóvis L da Silva, Magnólia L N Gauchée, Marina F M Tavares","doi":"","DOIUrl":"","url":null,"abstract":"This work presents a comparative evaluation of extraction procedures for the capillary analysis of seven opiates (meperidine, morphine, naloxone, tramadol, fentanyl, sufentanyl, and alfentanyl) in human hair. Pieces of hair (50-150 mg) were subjected to acidic hydrolysis (0.25 mmol L(-1) HCl at 45 degrees C, overnight) followed by pH adjustment and either liquid-liquid extraction (LLE) in hexane, petroleum ether, dichloromethane, and ethyl acetate solvents, or solid-phase extraction (SPE) in octadecyl, cyanopropyl, and aminopropyl bonded silica and cation exchange polymeric phases. Excellent recoveries of approximately 70% (naloxone and fentanyl and its analogues), 88% (meperidine), and ca. 100% (morphine and tramadol) were obtained using SPE in a M-fixed-mode cation exchange reversed-phase cartridge (Oasis MCX LP, Waters Corp., Milford, MA, U.S.A.), making this type of procedure eligible for novel clinical and forensic methodologies for hair analysis. The utility of the proposed extraction technique was demonstrated by the analysis of hair extracts from patients using morphine as part of their pain management protocol.","PeriodicalId":15060,"journal":{"name":"Journal of capillary electrophoresis and microchip technology","volume":"8 5-6","pages":"111-6"},"PeriodicalIF":0.0,"publicationDate":"2003-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"24684090","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

High-throughput genotyping by microchip electrophoresis. 芯片电泳高通量基因分型。