Journal of Bacteriology & Parasitology最新文献_第7页

Dynamics and Fitness Cost of Genetically Engineered Entrobacter cloacae Expressing Defensin for Paratransgenesis in Phlebotomus papatasi 在木瓜白蛉中表达防御素的基因工程泄殖腔内entrobacer的动力学和适应度成本

Journal of Bacteriology & Parasitology Pub Date : 2019-01-01 DOI: 10.35248/2155-9597.1000349

R. Abassi, M. Akhlaghi, M. Oshaghi, A. Akhavan, M. Yaghoobi-Ershadi, Rounak Bakhtiary, F. Mohtarami

{"title":"Dynamics and Fitness Cost of Genetically Engineered Entrobacter cloacae Expressing Defensin for Paratransgenesis in Phlebotomus papatasi","authors":"R. Abassi, M. Akhlaghi, M. Oshaghi, A. Akhavan, M. Yaghoobi-Ershadi, Rounak Bakhtiary, F. Mohtarami","doi":"10.35248/2155-9597.1000349","DOIUrl":"https://doi.org/10.35248/2155-9597.1000349","url":null,"abstract":"Background: Enterobacter cloacae subsp. dissolvens bacterium is a known commensal of the gut microflora of Phlebotomus papatasi, the main vector for zoonotic cutaneous Leishmaniasis, and nominated for paratransgenesis in sand flies. In this study, we evaluated dynamics and fitness costs of engineered E. cloacae for its potential to serve as a 'Trojan Horse' in P. papatasi. Methods: The engineered strain of E. cloacae transformed with a constantly active expressed red fluorescent protein plus defensin (EC-DR) plasmid and was fed to sand fly colonies via larval food to larvae. A wild type the bacterium (EC-WT) and intact food were used as controls. Fitness characters as well as dynamics of the EC-DR at various development stages of sand fly larvae were tested by plating homogenized specimens and counting fluorescent expressing colonies on the Tet-BHI agar medium. Results: Enterobacter cloacaeDR producing red fluorescent protein could be isolated from the larvae gut after 36 days when the bacteria were added once in larval pots. The EC-DR with multiple applications had no negative effect on emergence time of instar II larvae, pupae, and adults but increased slightly mortality rate of P. papatasi larvae. The experiment also confirmed lack or weak trans-stadial transmission of E. cloacae DR in P. papatasi. It has minimal fitness cost on P. papatasifeeding behavior and survival. Conclusion: Results of this study showed that E. cloacae DR is suitable for paratransgenesis of P. papatasi at only adult stage because it did not transmit transstadially.","PeriodicalId":15045,"journal":{"name":"Journal of Bacteriology & Parasitology","volume":"20 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"80599854","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 5

Prevalence and Intensity of Soil Transmitted Helminths among School Children in Ifetedo, Osun State, Nigeria 尼日利亚奥松州伊费特多学龄儿童土壤传播蠕虫的流行程度和强度

Journal of Bacteriology & Parasitology Pub Date : 2019-01-01 DOI: 10.35248/2155-9597.1000352

Oluwarotimi Ademola Omotola, I. E. Ofoezie

{"title":"Prevalence and Intensity of Soil Transmitted Helminths among School Children in Ifetedo, Osun State, Nigeria","authors":"Oluwarotimi Ademola Omotola, I. E. Ofoezie","doi":"10.35248/2155-9597.1000352","DOIUrl":"https://doi.org/10.35248/2155-9597.1000352","url":null,"abstract":"The study assessed the prevalence, intensity and risk factors affecting the transmission of soil transmitted helminthiasis among school-age children in Ifetedo, Osun State, Nigeria with the view to determining the patterns of transmission of the diseases in the area. The study involved stool examination for infective stages of soil-transmitted helminth parasites using some parasitological analysis. Ascariasis, Trichiuriasis and hookworms infection were the only soil transmitted helminthes (STHs) infections observed among the pupils. The overall prevalence of the diseases was 44.2% (175 of 396 pupils). The sex related prevalence showed that males (48.6%) were more infected than females (40.4%). The peak prevalence (45.9%) was recorded among the 6-9 years old age group and lowest (42.4%) among the youngest children (≤ 5 years old age group). Prevalence and intensity of STHs infection did not vary significantly (p>0.05) among the sexes and age groups. The study concluded that factors such as types of toilets in home and presence of human/animal faeces around home environment are risk factors for STHs infections in the area.","PeriodicalId":15045,"journal":{"name":"Journal of Bacteriology & Parasitology","volume":"2 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"80216158","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 7

Intestinal Tuberculosis Presenting as Massive Lower Gastrointestinal Bleeding: A Rare Presentation in Child 以大量下消化道出血为表现的肠结核:一种罕见的儿童表现

Journal of Bacteriology & Parasitology Pub Date : 2019-01-01 DOI: 10.35248/2155-9597.19.10.357

Aji Mathew, Yusuf Parvez, S. Thomas

{"title":"Intestinal Tuberculosis Presenting as Massive Lower Gastrointestinal Bleeding: A Rare Presentation in Child","authors":"Aji Mathew, Yusuf Parvez, S. Thomas","doi":"10.35248/2155-9597.19.10.357","DOIUrl":"https://doi.org/10.35248/2155-9597.19.10.357","url":null,"abstract":"Background: Intestinal tuberculosis is a form of extra pulmonary tuberculosis that can involve any part of the gastrointestinal tract. It can present with nonspecific symptoms like chronic abdominal pain, anorexia, fatigue, fever, night sweats, weight loss, diarrhea, constipation, or blood in the stools. Acute presentation including intestinal obstruction and perforation have been reported in the literature; however massive lower gastrointestinal bleeding as one of the presenting symptom is rare. The management includes anti-tubercular drugs along with surgical intervention in acute presentation. Case characteristic: 11-year-old girl was admitted with complaints of fever and vague abdominal pain for one-week duration. Observation/intervention: The child was having recent weight loss, hypo albuminemia and strongly positive Manteaux test. Ultrasound abdomen revealed multiple pre and para aortic lymphadenopathy. The Computerised tomography (CT) guided biopsy from lymph node was planned but the child developed massive lower gastrointestinal bleeding which was managed surgically. Conclusion: The child underwent hemi-colectomy with ileostomy and intestinal tuberculosis was confirmed by biopsy. The patient was discharged home on anti-tubercular drugs with further follow up. Message: Intestinal tuberculosis should be considered as one of the differential diagnosis in a child presenting with massive lower gastrointestinal bleeding.","PeriodicalId":15045,"journal":{"name":"Journal of Bacteriology & Parasitology","volume":"8 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"86407885","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Development and Testing of Trial Samples for Specific Serodiagnosis of Fasciola gigantica via External Quality Assessment 通过外部质量评估进行巨型片形吸虫特异性血清诊断的试验样品的开发和测试

Journal of Bacteriology & Parasitology Pub Date : 2019-01-01 DOI: 10.35248/2155-9597.1000351

V. Q. Huy, T. T. Trung, H. M. Tuan, V. T. Thanh, Le Van Chuong, Nguyen Lam Duc Vu, Huynh Thi Diem Phuc, B. Q. Sang

{"title":"Development and Testing of Trial Samples for Specific Serodiagnosis of Fasciola gigantica via External Quality Assessment","authors":"V. Q. Huy, T. T. Trung, H. M. Tuan, V. T. Thanh, Le Van Chuong, Nguyen Lam Duc Vu, Huynh Thi Diem Phuc, B. Q. Sang","doi":"10.35248/2155-9597.1000351","DOIUrl":"https://doi.org/10.35248/2155-9597.1000351","url":null,"abstract":"Objective: To build a procedure for production of samples for specific serodiagnosis of anti-Fasciola gigantica antibodies via an external quality assessment scheme, and to evaluate the homogeneity and stability of the trial samples. Methods: In this experimental study, samples contained an anti-Fasciola gigantica antibody according to screening by ELISA, followed by confirmation by Western blotting were collected. All samples were tested and found negative for other helminth, especially of trematode antibodies. The samples were also negative for anti-HIV-1 and -2 antibodies, anti-HCV antibodies, and HBs antigen. The samples were prepared by freeze-drying and freezing methods, the stability and homogeneity were evaluated each 2, 4, 8, 12 and up to 24 weeks. Results: We produced three lots of serum samples containing anti-Fasciola gigantica antibodies at three levels. Lot DK1 had optical density, OD=0.350 ± 0.037; Lots DK3 and DL3 had O=0.653 ± 0.046, and Lot DL1 had OD=0.850 ± 0.047 with the wavelength of 450 nm. The specific IgG antibodies against Fasciola gigantica antigens were found to be positive for all three proteins, 8–9 kDa protein (P 8-9), 28 kDa (P 28), and 42 kDa (P 42) by the Western blot technique. The trial samples were confirmed to be homogeneous by Fisher’s test (F statistics0.05) as well as to be stable during 24 weeks (with t statistics0.05). Conclusion: Trial samples for specific serodiagnosis of anti-Fasciola gigantica antibodies via external quality assessment can be produced with homogeneity and stability lasting for 24 weeks by freeze-drying and freezing methods.","PeriodicalId":15045,"journal":{"name":"Journal of Bacteriology & Parasitology","volume":"53 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"85632725","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

Prevalence of Intestinal Parasites among Students of a Tertiary Institution in Jos, Nigeria 尼日利亚乔斯一所高等院校学生肠道寄生虫患病率

Journal of Bacteriology & Parasitology Pub Date : 2019-01-01 DOI: 10.35248/2155-9597.19.10.360

O. Ejinaka, Obeta Mu, J. Ri, Lote-Nwaru Ie, Nkop Jp, PI Agbalaka., Friday Pe

{"title":"Prevalence of Intestinal Parasites among Students of a Tertiary Institution in Jos, Nigeria","authors":"O. Ejinaka, Obeta Mu, J. Ri, Lote-Nwaru Ie, Nkop Jp, PI Agbalaka., Friday Pe","doi":"10.35248/2155-9597.19.10.360","DOIUrl":"https://doi.org/10.35248/2155-9597.19.10.360","url":null,"abstract":"A tertiary institution in Jos that admits students who are resident in the hostel where sources of water are from borehole, rain and commercial sachet water had complains from the students as a result of stomach pains and discomfort. The prevalence of intestinal parasites and most prevalent parasite among students of Federal School of Medical Science, Jos through experimental study was conducted among all the students available in the hostel of the tertiary institution in the month of September 2017 and analyzed with Percentages. Sixty faecal samples were examined for intestinal parasites by Macroscopy, Microscopy: direct and formol-ether concentration techniques. Twenty six (26) out of sixty samples were positive for intestinal parasites giving a prevalence of 43.3%. The age groups 15-20 and 21-25 years had the highest prevalence of 34.6%. Age groups between 31-35 had the lowest of 3.8%. Parasites identified were Ascaris lumbricoides (69.2%) hookworm (15.4%) and Schitosoma mansoni (15.4%). This study shows that students who use borehole (65.4%) were more infected than those drinking rain water (15.4%) and sachet water (19.2%). The prevalence in males were (26.9%) and females (73.1%) than those who washed their vegetables (23.1%). Students of Federal School of Medical Laboratory Science, Jos had a parasite prevalence of 43% of which Ascaris lumbricoides was more prevalent (69.2) followed by Hookworm (15.4) and Schistosoma mansoni (15.4). The management of the school should provide safe water and adequate education for prevention.","PeriodicalId":15045,"journal":{"name":"Journal of Bacteriology & Parasitology","volume":"11 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"74867438","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

Community-Acquired Methicillin-Resistant Staphylococcus aureus Severe Infection in Two Healthy Patients with Ear Piercing 两例健康耳穿刺患者社区获得性耐甲氧西林金黄色葡萄球菌严重感染

Journal of Bacteriology & Parasitology Pub Date : 2019-01-01 DOI: 10.35248/2155-9597.19.10.356

E. Méndez, M. Garberi, M. Baroni, Maria Alej, ra Mendosa, Glenda Segovia, S. Cristobal, A. Mollerach, A. Nagel, G. D. Giovanni

引用次数: 1

Efficacy of Chemical Reagent Strip in the Diagnosis of Urinary Schistosomiasis in Ikota, Ifedore Local Government Area, Ondo State, Nigeria 尼日利亚奥多州伊科塔州伊费多多地方政府区化学试剂试纸对尿路血吸虫病的诊断效果

Journal of Bacteriology & Parasitology Pub Date : 2019-01-01 DOI: 10.35248/2155-9597.19.10.354

Awosolu Ob, Adesina Fp, E. Os, Akinnifesi Oj

{"title":"Efficacy of Chemical Reagent Strip in the Diagnosis of Urinary Schistosomiasis in Ikota, Ifedore Local Government Area, Ondo State, Nigeria","authors":"Awosolu Ob, Adesina Fp, E. Os, Akinnifesi Oj","doi":"10.35248/2155-9597.19.10.354","DOIUrl":"https://doi.org/10.35248/2155-9597.19.10.354","url":null,"abstract":"Urinary schistosomiasis is a parasitic disease caused by Schistosoma haematobium which affects the bladder and as such is a major source of morbidity and mortality in Africa and Sub-Sahara Africa. This study was conducted to determine the efficiency and efficacy of chemical reagent strip in the diagnosis of urinary schistosomiasis among pupils in Ikota, Ifedore Local Government Area of Ondo state. The pupils’ data were obtained using pretested, well-structured questionnaire while the samples were analyzed in the laboratory using sedimentation method and chemical reagent dipstick (Combi-9) was used to examine the microhaematuria and proteinuria. Of the one hundred and fifty (150) individuals that were sampled, 76 (50.66%) pupils were males and 74 (49.33%) were females. The prevalence of infection of S. haematobium in the area was 36 (24.0%). There was no significant difference (p >0.05) in prevalence relating to sex and age. Analysis on microhaematuria and proteinuria shows that 30 (20.0%) were positive for microhaematuria and 76 (50.67%) for proteinuria. The sensitivities of the urinary symptoms were 50.0% for proteinuria and 83.33% for microhaematuria and specificity was 62.3% for proteinuria and 100.00% for microhaematuria respectively. This chemical reagent strip combine with gold standard method of diagnosis will increase the accuracy and give good predictive value.","PeriodicalId":15045,"journal":{"name":"Journal of Bacteriology & Parasitology","volume":"54 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"80752564","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 4

Development of Neutralizing Buffered Peptone Water for Salmonella Verification Testing in Commercial Poultry Processing Facilities 用于商业家禽加工厂沙门氏菌验证试验的中和缓冲蛋白胨水的研制

Journal of Bacteriology & Parasitology Pub Date : 2019-01-01 DOI: 10.35248/2155-9597.19.10.359

A. Hinton, G. Gamble, M. Berrang, R. J. Buhr, J. Johnston

引用次数: 3

Incidence of Isospora belli in Leukemic Children in Erbil City 埃尔比勒市白血病儿童腹部异孢子虫的发病率

Journal of Bacteriology & Parasitology Pub Date : 2019-01-01 DOI: 10.35248/2155-9597.19.10.361

W. Al-Saeed, H. M. Abdullah

引用次数: 0

Detection of the Panton-Valentine Leukocidin Gene in Swedish Isolates of Methicillin-Resistant Staphylococcus aureus using a Multiplex PCR Assay 多重PCR法检测瑞典耐甲氧西林金黄色葡萄球菌潘通-瓦伦丁嗜白细胞素基因

Journal of Bacteriology & Parasitology Pub Date : 2019-01-01 DOI: 10.35248/2155-9597.19.10.353

W. Abu Al-Soud

引用次数: 2