Journal of Applied Biotechnology & Bioengineering最新文献

{"title":"Evaluation of responses embryogenic Cycas revoluta thumb., from callus culture obtained in vitro","authors":"Rodriguez de la O JL,  Lazarus J Flores VA, Robledo Monterrubio MS, Contreras Cruz F","doi":"10.15406/jabb.2019.06.00192","DOIUrl":"https://doi.org/10.15406/jabb.2019.06.00192","url":null,"abstract":"Processes cell differentiation and dedifferentiation are included in the development of biotechnology protocols to promote somatic embryogenesis as an alternative to the in vitro propagation of plants, somatic embryos may be an excellent strategy for both propagation and conservation of fossil species such as cycads. They were evaluated in vitro with different strategies, morphogenic responses associated with obtaining somatic embryos of C. revolute. calli of megagametophytes, subsequently subcultured in four combinations of basic salts of Murashige and Skoog (1962) MS, with the addition of benzyladenine (BA), and 2, dichlorophenoxyacetic (2,4-D) were used, and kinetin (K), and picloram. In the results, It was possible to characterize potentially embryogenic callus, evaluating the levels of both cellular differentiation, necrosis, texture and color; and increases mass or weight considered to start differentiation or proembryogenic or globular type. Calli were subcultured in a medium containing MS salts, incorporating abscisic acid (ABA) in 0, 0.38, 1.13, 3.78 and 5.67 uM doses influenced both the production and maturation of somatic embryos. Embryonic structures, presented a pink coloration characteristic strongly associated towards maturity. The effect of combinations of BA, Kin, 2,4-D, GA3 and ANA influenced the development and germination of mature somatic embryos. And the combination of 1.36 mM 2,4-D+4.44 uM BA promoted the appearance of calluses with a compact texture, characteristic related to their embryogenic potential. The purpose of this research in Cycas sp was to contribute to the study of the in vitro morphogenic responses of this group of plants. And somatic embryogenesis, will allow the obtaining and multiplication as well as its preservation of Cycas sp. Gender that is evolutionary very important","PeriodicalId":15033,"journal":{"name":"Journal of Applied Biotechnology & Bioengineering","volume":"26 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-08-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"90739660","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Production of Succinic Acid: Effects of C:N Ratio","authors":"T. A. Gonzales, L. O. Santos, Beatriz Torsani Úbeda, R. Alegre, Rosana Goldbeck","doi":"10.5296/JAB.V7I2.14867","DOIUrl":"https://doi.org/10.5296/JAB.V7I2.14867","url":null,"abstract":"In this work, the effects of different carbon:nitrogen (C:N) ratios, carbon source and initial carbon concentration, in liquid medium used for submerged fermentation, on biomass growth, succinic acid production and yield of product in substrate (YP/S) were studied. It was used three carbon sources as substrate such as glucose, xylose and sucrose and the C:N ratios studied were in the range of 2.5-35. The succinic acid production was best at a C:N ratio of 12.5:1, as much for the succinic acid production as for the yield of product in relation to the substrate (YP/S). For the biomass growth the C:N ratios of 2.5:1, 5:1 and 10:1 had only presented significant difference with regards to the too much assays. The substrate that presents the best results was the glucose with succinic acid concentrations up to 7.5 g.L-1.","PeriodicalId":15033,"journal":{"name":"Journal of Applied Biotechnology & Bioengineering","volume":"43 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-08-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"79378638","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Moringa oleifera Seeds Extract Activity on Enteropathogenic Escherichia coli and Aeromonas hydrophyla Cells in Aquatic Microcosm","authors":"C. S. Metsopkeng, C. L. Djimeli, O. N. Ewoti, L. M. Moungang, P. Nana, A. T. Arfao, P. N. Bahebeck, T. Sime-Ngando, M. Nola","doi":"10.5296/jab.v7i2.14917","DOIUrl":"https://doi.org/10.5296/jab.v7i2.14917","url":null,"abstract":"This study aimed to evaluate in microcosm condition, the survival of Aeromonas hydrophila and Enteropathogenic Escherichia coli (EPEC), in the presence of M. oleifera aqueous seeds extract at concentrations varying from 1 to 40 g/L, and under 4 °C and 23 °C incubation temperature. It has been noted that cell abundances decrease gradually with the increasing in the seeds extract concentration. However, a marked cells regrowth was sometimes noted. In monospecies cell incubation condition, under 4 °C, the EPEC cells inhibition percentages (CIP) values varied from 52.12 to 99.84%. Those of A. hydrophila varied from 13.2 to 96%. The lowest CIPs were noted at the extract concentration 1g/L for EPEC and A. hydrophila. The highest CIP value was registered at 10 and 40 g/L for EPEC and at 15 g/L for A. hydrophila. Under 23 °C incubation, the EPEC CIPs values varied from 74.04 to 99.9% and those of A. hydrophila varied from 21.2 to 97.8%. For E. coli, the lowest and the highest CIP were recorded at the extract concentration 1g/L and 30 g/L, respectively. In bispecies cells incubation condition, the CIPs were relatively different. These results show the potential exploitation of M. oleifera extracts in the microbiological treatment of potable water.","PeriodicalId":15033,"journal":{"name":"Journal of Applied Biotechnology & Bioengineering","volume":"38 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-08-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"83811824","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Improvement in cell growth and ethanol productivity of Saccharomyces cerevisiae due to poultry litter biochar","authors":"Foster A Agblevor, Oumou T. Diallo","doi":"10.15406/jabb.2019.06.00191","DOIUrl":"https://doi.org/10.15406/jabb.2019.06.00191","url":null,"abstract":"The effect of poultry litter biochar on Saccharomyces cerevisiae growth and ethanol fermentation was investigated for glucose, steam exploded corn stover and poplar wood. S. cerevisiae grew faster in both anaerobic and aerobic biochar medium than the control media. For the same initial concentration of glucose, the biochar medium glucose was consumed in 12 hours compared to 24 hours for control and the maximum ethanol productivity were higher for the biochar compared to the control medium. When the initial glucose concentration was increased the maximum ethanol productivity for the biochar media was more than two times that for the control. Similarly, addition of poultry litter biochar to steam exploded poplar wood and corn stover improved the ethanol productivity depending on the amount of biochar added to the fermentation medium. Thus, biochar is an effective additive to reduce ethanol fermentation time","PeriodicalId":15033,"journal":{"name":"Journal of Applied Biotechnology & Bioengineering","volume":"8 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-07-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"77972941","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Influence of drinking structured water to human psychophysiology","authors":"K. Korotkov, O. Churganov, Elena Gavrilova, Belodedova Ma, Korotkova Ak","doi":"10.15406/jabb.2019.06.00190","DOIUrl":"https://doi.org/10.15406/jabb.2019.06.00190","url":null,"abstract":"Aim: The aim of the study was to study the impact of the consumption of structured water on the human body in normal environmental conditions during one month. Materials and methods: The following techniques have been used in the study: study of body composition by bioelectric impedance; clinical and biochemical blood analysis; psychological testing; heart rate variability (HRV) method; Bio-Well GDV method. This study was an open, randomized, comparative study in which the main performance indicators were evaluated in 15 subjects - an experimental group using structured water - 1 liter per day for a month, compared to 15 subjects in the control group (using unstructured water of the same brand for a month). . Comparative tests were carried out initially and after 1 month. Results: Both groups demonstrated an increase in the average concentration of hemoglobin in erythrocytes, which indicated an improvement in water-salt metabolism, which was due to the regular water consumption regardless of its structure. The experimental group revealed a significant reduction in total body weight due to reduction of fat mass; a significant decrease in creatinine levels, as well as an increase in glomerular filtration of the kidneys, which reflects the improvement of renal excretory function and a decrease in the level of endogenous intoxication of the body; the growth of HRV and Bio-Well GDV parameters reflecting parasympathetic activity and variability of heart rhythm and the decrease of parameters which reflect the degree of sympathetic regulation and centralization of heart rhythm. No such changes were observed in the control group, but there was a reliable negative dynamics of parameters for most of participants. Conclusions: From the analysis of experimental data was concluded that structured water consumed 1 liter per day for a month contributes to the reduction of body fat mass, improvement of water-salt metabolism, improvement of kidney excretory function, reduction of endogenous intoxication of the body, increase of adaptive and stress-response and reserve capacity of the body even in adverse seasonal, climatic and epidemiological period. In contrast, in the control group there was a reliable negative dynamics of parameters for most of participants. The experiment was conducted during the unfavorable seasonal, climatic and epidemiological period - the period of seasonal immunodeficiency, depression and reduced efficiency. At the same time, this investigation should be considered as a pilot study, as the sample size (n=30) is too small to draw any decisive conclusions. Since the study includes different age groups, sexes, and profession with that small sample size, it becomes even more complicated to draw any conclusions. We hope, that presented results stimulate development of new studies, dedicated to the influence of water to human health and wellbeing.","PeriodicalId":15033,"journal":{"name":"Journal of Applied Biotechnology & Bioengineering","volume":"18 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-07-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"85619699","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Mechanisms of programmed cell death","authors":"Abdu-Alhameed A Ali Azzwali,  Azab Elsayed Azab","doi":"10.15406/jabb.2019.06.00188","DOIUrl":"https://doi.org/10.15406/jabb.2019.06.00188","url":null,"abstract":"The present review aims to spotlight on the mechanisms and stages of programmed cell death. Apoptosis, known as programmed cell death, is a homeostatic mechanism that generally occurs during development and aging in order to keep cells in tissue. It can also act as a protective mechanism, for example, in immune response or if cells are damaged by toxin agents or diseases. In cancer treatment, drugs and irradiation used in chemotherapy leads to DNA damage, which results in triggering apoptosis through the p53 dependent pathway in cancer treatment, drugs and irradiation used in chemotherapy leads to DNA damage, which results in triggering apoptosis through the p53 dependent pathway. Corticosteroids can cause apoptotic death in a number of cells. A number of changes in cell morphology are related to the different stages of apoptosis, which includes nuclear DNA fragmentation, cell shrinkage, chromatin condensation, membrane blebbing, and the formation of apoptotic bodies. There are three pathways for apoptosis, the intrinsic (mitochondrial) and extrinsic (death receptor) are the two major paths that are interlinked and that can effect one another. Conclusion: It can be concluded that apoptosis is a homeostatic mechanism that generally occurs during development and aging in order to keep cells in tissue. Drugs and irradiation used in chemotherapy leads to DNA damage, which results in triggering apoptosis through the p53 dependent pathway. The apoptosis, stages are includes nuclear DNA fragmentation, cell shrinkage, chromatin condensation, membrane blebbing, and the formation of apoptotic bodies. There are three pathways for apoptosis.","PeriodicalId":15033,"journal":{"name":"Journal of Applied Biotechnology & Bioengineering","volume":"26 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-07-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"85847153","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Assessing Productivity and Profitability of Hybrid Rice With Site Specific Nutrient Management in Mid Western Terai, Nepal","authors":"S. Acharya, Binita Mahara, L. Amgain, K. Aryal, Bishnu l Prasad Kande","doi":"10.5296/JAB.V7I2.14959","DOIUrl":"https://doi.org/10.5296/JAB.V7I2.14959","url":null,"abstract":"Rice (Oryza sativa L.) is a dominant staple food crop of Nepal which production and productivity is significantly declining compared to several years due to inappropriate nutrient management practices. A field experiment was conducted at Lamahi, Dang to evaluate the performance of hybrid rice(US-305) under rain fed condition with five precision nutrient management practices [Viz: Nutrient Expert® -Rice (NE) recommendation; Leaf Color Chart (LCC) N and Nutrient Expert (P and K); Nutrient Expert (N) and Farmers Fertilizer Practices (P and K); Farmers Fertilizer Practices (FFP) and Government Recommendation (GR)] replicated four times in RCBD design during June to October, 2018. The experimental finding showed that SSNM based Nutrient Expert® -Rice (NE) recommendation gave higher grain yield (6.36 ton ha-1) and straw yield (12.62 ton ha-1) which leads to highest gross return (NRs 242,498) and B: C ratio(3.08). Between the treatments Nutrient Expert® -Rice (NE) recommendation was excellent to growth parameters like plant height, crop growth rate, relative growth rate and leaf area index over FFP. Further, Nutrient Expert® -Rice (NE) recommendation gave significantly higher effective tiller m-2(354.50), panicle length (26.31), panicle weight (81.50), filled grain (390) and fertility (87.56%) over FFP. Nutrient Expert® -Rice (NE) recommendation has increased the grain yield by 23.97% with yield difference of 1.23 ton ha-1 and straw yield by 39.44 % with yield difference of 3.57 ton ha-1 in comparison with FFP. Hence the experiment concluded that site specific nutrient management recommendation that accounts Nutrient Expert® -Rice and leaf color chart could be the practical decision tool for making authentic fertilizer recommendation.","PeriodicalId":15033,"journal":{"name":"Journal of Applied Biotechnology & Bioengineering","volume":"19 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-06-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"89815430","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Robustness and Ethanol Production of Industrial Strains of Saccharomyces cerevisiae Using Different Sugarcane Bagasse Hydrolysates","authors":"Vanessa Siqueira de Castro Teixeira, Suéllen Azambuja, P. H. Carvalho, F. Costa, P. R. Kitaka, Claudia Stekelgerb, S. R. Andrietta, M. Andrietta, Rosana Goldbeck","doi":"10.5296/JAB.V7I1.14599","DOIUrl":"https://doi.org/10.5296/JAB.V7I1.14599","url":null,"abstract":"Sugarcane bagasse is one of the main lignocellulosic raw materials used for the production of second-generation ethanol. Technological studies on fermentation processes have focused on the search for and development of more robust microorganisms that are able to produce bioethanol efficiently and are resistant to the main fermentation inhibitors. The purpose of this study was to evaluate the robustness and ethanol production of industrial strains of Saccharomyces cerevisiae using acid, alkaline, and enzymatic sugarcane bagasse hydrolysates. Hydrolysis was carried out to release fermentable sugars from sugarcane bagasse. Fermentations were performed in shake flasks containing sugarcane hydrolysates supplemented with 150 g L−1 glucose to evaluate the kinetic parameters of the reaction. Inhibitor tolerance was evaluated by incubating cells with different concentrations of inhibitors in 96-well plates. The biomass yield on substrate, ethanol yield on substrate, and ethanol productivity of the six strains were higher in 0.5% acid, 0.5% alkaline, and enzymatic hydrolysates (i.e., under milder conditions). The SA-1 (Santa Adélia-1) strain had a better performance in comparison with the other strains for its ability to produce ethanol in a very severe condition (7% acid hydrolysis) and for its robustness in growing at several inhibitor concentrations.","PeriodicalId":15033,"journal":{"name":"Journal of Applied Biotechnology & Bioengineering","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-05-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"75282027","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Sinoatrial node pacemaker cell pool dynamics upon synchronization with vagus nerve rhythm","authors":"krovskii Vm, Nechepurenko Aa,  Tarasov Dg, Korotkov Kg, Abushkevich Vg","doi":"10.15406/jabb.2019.06.00182","DOIUrl":"https://doi.org/10.15406/jabb.2019.06.00182","url":null,"abstract":"Aim: To reveal the dynamics of sinoatrial node pacemaker cell pools upon synchronization with vagus nerve rhythm through the model of vagal-cardiac synchronization. Materials and methods: Observations were carried out on 10 narcotized cats. The animals were tracheostomized and pump-ventilated, and the pericardium accessed via an open-chest transsternal incision. A device (KELSY scanner manufactured by Elsys, St. Petersburg, Russia) accompanied by a microscope and a video-camera, to visualize the luminescence of excitation processes in the sinoatrial node in a high-frequency electromagnetic field (1024 Hz) was placed in the sinoatrial area of a working heart. Luminescent focus in the sinoatrial node was registered as a peripherally cut end of the vagus nerve was stimulated with bursts of electrical impulses (5 impulses, 2 ms, 20 Hz) from an electrostimulator. Results: Luminescence localized at the entrance of the cranial vena cava was visualized in a high-frequency electrical field in narcotized cats. The luminescent focal area was not homogenous and looked like a number of luminescent pools. Upon vagal-cardiac synchronization caused by the stimulation of a peripherally cut end of the vagus nerve with bursts of electrical impulses, the focus was wide and solid. Conclusion: Here, pacemaker cell dynamics were studied in the feline heart. When vagal- cardiac synchronization was activated, synchronization of the heart with vagus nerve rhythm was accompanied by an increase in the early depolarization area in the sinoatrial area of the feline heart. The mechanisms underlying heart rate synchronization are not clearly defined. Rhythm is achieved through actions of the SA node and the vagus nerve. Our data confirm the vagal- cardiac synchronization model.","PeriodicalId":15033,"journal":{"name":"Journal of Applied Biotechnology & Bioengineering","volume":"33 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-05-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"76465239","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Therapeutic proteases from plants: biopharmaceuticals with multiple applications","authors":"R. E. Silva-López, Rayane Natashe Gonçalves","doi":"10.15406/JABB.2019.06.00180","DOIUrl":"https://doi.org/10.15406/JABB.2019.06.00180","url":null,"abstract":"Human beings have used plants to treat diseases and adverse conditions since ancient times, and consequently, employed their proteases empirically.1 Nowadays, proteases represent a very significant group of industrial enzymes, accounting for 60% of the total enzyme market, especially the proteases from microorganisms due to ease of modifying their biochemical characteristics through genetic mutations.2 In addition, plant proteases are part of a growing class of therapeutic agents because many efforts have been made to exploit biodiversity.3 The Table 1 summarize some application of general proteases in different kinds of industry. 4–6","PeriodicalId":15033,"journal":{"name":"Journal of Applied Biotechnology & Bioengineering","volume":"18 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-03-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"72891668","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}