Journal of Applied Biology and Biotechnology最新文献

{"title":"Effect of extraction methods and temperature preservation on total anthocyanins compounds of Peristrophe bivalvis L. Merr leaf","authors":"Thuyet Nguyen Minh, Han Dao Huynh Ngoc, Minh Vo Quang, Tai Ngo Van","doi":"10.7324/jabb.2022.100218","DOIUrl":"https://doi.org/10.7324/jabb.2022.100218","url":null,"abstract":"This paper proposes anthocyanins extraction from Peristrophe bivalvis L. Merr. (“lá cẩm”) and preservation. Experiments were performed in water as a solvent using two different extraction methods [conventional extraction (CE) and microwave-assisted extraction (MAE)], including different solvent-to-raw material ratios, temperatures/microwave power, and times of extraction. The total anthocyanin in the extract was analyzed by UV-Vis. Multiple regression analysis was applied to analyze the influence of factors and select the optimal parameters for each extraction process. The influence of storage conditions on the quality of extracts from “lá cẩm” leaves was controlled. It was observed that the ratio of solvent and fresh leaf used, temperature/ microwave power, and extraction time all affected the anthocyanin content recovered from the extract when using different extraction techniques. The MAE technique gave superior results compared to the CE method. The solvent used was slightly higher and the extraction time was 6.2 times shorter than that of the CE method. The optimal parameters of each extraction technique are selected. With the CE method, the solvent-to-fresh leaf ratio was 11.22:1; the optimum temperature and time were 88°C and 27.21 minutes resulting in the anthocyanin content in the extract being 25.61 mg/g dry weight basic (db). Meanwhile, with the MAE method, the solventto-fresh leaf ratio used was 10.83:1 (v/w), the microwave power and the optimal extraction time were 600 W and 4.39 minutes, and the obtained extract had an anthocyanin content of 30.97 mg/g db. In addition, the extract was best preserved in the dark and the storage temperature was −9°C. The remaining anthocyanin content after 30 days of storage was 92.35%. The anthocyanin degradation kinetics was also analyzed. Changes in total anthocyanin followed a zero-order reaction kinetic model. The potential of the half-life of anthocyanin was 221, 114, and 19 days at −9°C, 4°C, and 28°C, respectively.","PeriodicalId":15032,"journal":{"name":"Journal of Applied Biology and Biotechnology","volume":"32 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-02-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"83170754","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Diversity due to mutations in circulating virus strains of SARS-CoV-2 may delay control of COVID-19","authors":"Patil Sharanagouda S., S. Chandan, Dharmashekar Chandan, P. Sushma., Suresh Kuralayanapalya Puttahonnappa, Prasad Ashwini, K. Shiva Prasad, Yadav Mahendra P., S. Chandrashekar, Pattnaik Bramhadev","doi":"10.7324/jabb.2022.100223","DOIUrl":"https://doi.org/10.7324/jabb.2022.100223","url":null,"abstract":"Severe acute respiratory syndrome (SARS)-coronavirus-2 (CoV-2) is a beta-coronavirus (beta-CoV;sarbecovirus), like its predecessors SARS and MERS CoVs. Of the structural proteins of the virus, the Spike (S) protein on the virion envelope binds to the host cell ACE2 through viral epitopes in the receptor-binding domain (RBD). Deletions in the ORF8 as well as mutations in the S gene of SARS-CoV of 2003 were related to adaptation of the virus to humans. The emergence of novel variants of SARS-CoV-2, viz., B.1.1.7, B.1.427 and B.1.429, B.1.617 and its Kappa and Delta strains/ variants, B.1.351, and P.1 in the United Kingdom, Americas, India, South Africa and Brazil, respectively, has been found be associated with the current waves of the COVID-19 pandemic. These variants are antigenically dissimilar, whereas the current COVID-19 vaccines are monovalent. This is a handicap in the control program. The Delta variant has been reported in 74 countries as of 14 June 2021 and the anticipated third wave involving this variant is of concern to the countries (www.gavi.org). Of late, on 17 June 2021, Delta Plus variant was identified in India (AIIMS, Bhopal, India). Circulation of virus in vaccinated population may lead to endemicity, and this can be monitored by regular serosurveillance for antibodies against select non-structural proteins (NSPs) of the virus;antibodies to NSPs will indicate virus replication in the host. Endemic areas will have higher NSP reactors. It is predicted that the Delta B.1 variant may ignite the third wave of the disease in many countries. As it has been difficult to achieve uniformity in time and density of the vaccination even in the districts, circulation of the virus in partially immune population may lead to the selection of newer variants of SARS-CoV-2. The presence of monoclonal antibody resistant mutants and neutralization—escape mutants in quasispecies structure of another + sense RNA virus, i.e., Aphthovirus (FMD virus;foot and mouth disease virus) in the family Picornaviridae is well documented. The situation could be similar in the Coronaviridae member SARS-CoV-2. Previous immunity may not protect against current/ future mutants thereby pro-longing the COVID-19 control Programme. © 2022 Sharanagouda S. Patil et al.","PeriodicalId":15032,"journal":{"name":"Journal of Applied Biology and Biotechnology","volume":"5 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-02-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"86448830","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Isolation and identification of potent tributyltin chloride utilizing Pseudomonas sp. strain VBAK from Vishakhapatnam shipping harbor sediments","authors":"Kumar Battu Anand, Gummadi Triveni, Gundluri Bhargava Ram, Berde Chanda Vikrant, Pallaval Veera Bramhachari","doi":"10.7324/jabb.2022.100203","DOIUrl":"https://doi.org/10.7324/jabb.2022.100203","url":null,"abstract":"Screening for tributyltin (TBT)-resistant and degrading bacteria was pertinent for the selection of isolates with decontamination ability of TBT. With this rationale, 205 strains were isolated from Vishakhapatnam shipping harbor sediments and their tolerance limit to TBT was evaluated in minimal salts media containing 2 mM TBT as the sole carbon source. Fourteen selective TBT-resistant isolates showed cross-tolerance to heavy metals and antibiotics. Among them, one interesting isolate VBAK, showing the highest TBT resistance and maximum growth yield, was selected and taxonomic identification was carried out by sequencing 16S rRNA gene. The maximum composite likelihood algorithm formed a coherent cluster with clad comprised Pseudomonas sp. The thin-layer chromatography profile of the degradation product revealed the depletion of TBT into dibutyltin. The Rf values of TBT and transformed compounds were 0.8 (solvent front = 16.5 ± 2, TBT 9.1 ± 1.0) and 0.7 (solvent front = 6.5 ± 2, product 6.8 ± 2), respectively. These results suggest that Pseudomonas sp. strain VBAK is potentially useful for bioremediation of TBT contamination.","PeriodicalId":15032,"journal":{"name":"Journal of Applied Biology and Biotechnology","volume":"3 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-02-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"82396457","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Antibacterial activity and hormetic response of silver nanoparticles synthesized using leaflet extract of wheat (Triticum aestivum) and rice (Oryza sativa) crop plants","authors":"Pahal Vikas, K. Pankaj, Kumar Parveen, K. Vinod","doi":"10.7324/jabb.2022.100219","DOIUrl":"https://doi.org/10.7324/jabb.2022.100219","url":null,"abstract":"Vikas Pahal1* , Pankaj Kumar2, Parveen Kumar3 , Vinod Kumar4 1Department Of Microbiology, Dolphin PG College of Science and Agriculture, Chunni Kalan, Fatehgarh Sahib, Punjab, India. 2Department Of Microbiology, Dolphin PG Institute of Biomedical and Natural Sciences, Dehradun, India. 3Bio-nanotechnology Lab, Central Scientific Instruments Organization, Chandigarh, India. 4Department of Pharmacology and toxicology, National Institute of Pharmaceutical Education and Research, S.A.S. Nagar, Mohali, India.","PeriodicalId":15032,"journal":{"name":"Journal of Applied Biology and Biotechnology","volume":"14 5 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-02-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"77316023","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Growth-promoting effects of marine microalgae species using tropical forest soil extracts","authors":"Arumugam Kasturi, Y. Nor Suhaila, A. Hasdianty, A. Mohd Fadzli, Maniyam Maegala Nallapan, Hashim Emi Fazlina, Sjahrir Fridelina, Ikram Wan Muhammad, Komatsu Kazuhiro, Kuwahara Victor S.","doi":"10.7324/jabb.2022.100216","DOIUrl":"https://doi.org/10.7324/jabb.2022.100216","url":null,"abstract":"Kasturi Arumugam1, Nor Suhaila Yaacob2*, Hasdianty Abdullah2, Mohd Fadzli Ahmad1, Maegala Nallapan Maniyam3, Emi Fazlina Hashim4, Fridelina Sjahrir1, Wan Muhammad Ikram1, Kazuhiro Komatsu5, Victor S. Kuwahara4 1Faculty of Engineering Life Sciences, Department of Science Biotechnology, Universiti Selangor, Selangor, Malaysia. 2Institute of Bio-IT Selangor, Universiti Selangor, Selangor, Malaysia. 3Centre for Foundation and General Studies, Universiti Selangor, Selangor, Malaysia. 4Faculty of Education Graduate School of Engineering, Soka University, Hachioji-Shi, Japan. 5Regional Environmental Conservation Division, National Institute for Environmental Studies, Tsukuba, Japan.","PeriodicalId":15032,"journal":{"name":"Journal of Applied Biology and Biotechnology","volume":"59 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-02-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"84920847","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Partial identification of a peptide from Lacticaseibacillus casei using MALDI TOF and it’s cytotoxic activity against MDA-MB-231 cell line","authors":"Siddique Jannatul Firdous, V. Mohanasrinivasan","doi":"10.7324/jabb.2022.100206","DOIUrl":"https://doi.org/10.7324/jabb.2022.100206","url":null,"abstract":"The current study focused to extract and purify the peptide of interest efficaciously from Lacticaseibacillus casei. The peptide of interest was partially identified and checked for anti-cancer activity using MDA-MB-231 triple negative breast cancer cell line. To begin with, it was purified via ammonium sulfate precipitation, ultrafiltration, and gel filtration chromatography. It was partially identified using mass matrix assisted laser desorption/ ionization-mass spectrometry (MS-MALDI) and checked for cytotoxic activity using (3-(4,5-Dimethylthiazol2-yl)-2,5-Diphenyltetrazolium Bromide) assay. As an outcome, the active part collected after ultra-filtration, 880 μg/ml of protein content with 15 mm zone diameter, was loaded on sephadexG50 column. The purified protein content was found to be 118 μg/ml with 15 mm of zone of inhibition against Listeria monocytogenes. A peak was obtained at a retention time of 9.371 minutes on subjected to high performance liquid chromatography. The result of MS-MALDI revealed it as an unnamed peptide with 75% query sequence similarity with the hypothetical protein of Lactobacillus reuteri. The cytotoxic study revealed its potential as an anti-cancer peptide, with the IC50 value of 108 μg/ml for MDA-MB-231 cancer cell line. In conclusion, this is the first report of this unknown peptide which showed both antibacterial and cytotoxic activity. It may act as a promising agent and can be further explored in future.","PeriodicalId":15032,"journal":{"name":"Journal of Applied Biology and Biotechnology","volume":"59 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-02-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"89162358","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Evaluation of the biosorption potential of Aspergillus flavus biomass for removal of chromium (VI) from an aqueous solution","authors":"Kapoor Riti Thapar","doi":"10.7324/jabb.2022.100208","DOIUrl":"https://doi.org/10.7324/jabb.2022.100208","url":null,"abstract":"Chromium biosorption potential of live and dead biomass of Aspergillus flavus was analyzed by batch experiments under various experimental conditions like pH, adsorbent dose, exposure period, and temperature. Maximum biosorption of hexavalent chromium was observed at pH 3.5 with adsorbent dose 2.5 g at 30°C. Three days were considered as the optimum exposure period for chromium removal for live biomass, whereas 1.3 hours exposure period for dead biomass of A. flavus. The equilibrium data were examined by Langmuir and Freundlich isotherms. Freundlich isotherm appeared to be the best fit model. Phytotoxicity test was conducted to check the effect of the treated chromium solution on the seed germination, seedling length, and vigor index of Vigna radiata. Only 23% germination was reported in chromium metal-treated V. radiata seeds, but germination and growth parameters of mung bean seeds were significantly increased in the chromium solution after treatment with dead and live biomass. The chromium biosorption potential showed the following trend: dead A. flavus > live A. flavus. Hence, live and dead biomass of A. flavus can be applied as a safe and economically feasible biosorbent for hexavalent chromium elimination for the treatment of industrial effluent or wastewater system.","PeriodicalId":15032,"journal":{"name":"Journal of Applied Biology and Biotechnology","volume":"40 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-02-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"88438196","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Molecular detection of Haemophilus influenzae isolated from eye swabs of patients with conjunctivitis in Hilla Province, Iraq","authors":"Al-Buhilal Jundi Alak Mahdi, Rhumaid Abdulkadir Kareem, Al-Tabtabai Alyaa Mohammed H., AL-Rubaey Niran Kadhim F.","doi":"10.7324/jabb.2022.100220","DOIUrl":"https://doi.org/10.7324/jabb.2022.100220","url":null,"abstract":"Jundi Alak Mahdi Al-Buhilal1, Abdulkadir Kareem Rhumaid1, Alyaa Mohammed H. Al-Tabtabai2, Niran Kadhim F. AL-Rubaey3* 1Department of Technical Medical Laboratories, Institute of Medical Technology Al-Mansour, Middle Technical University, Baghdad, Iraq. 2Department of Biotechnology, College of Science, University of Baghdad, Baghdad, Iraq. 3Department of Microbiology, Hammurabi College of Medicine, University of Babylon, Babylon, Iraq.","PeriodicalId":15032,"journal":{"name":"Journal of Applied Biology and Biotechnology","volume":"26 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-02-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"83294578","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Utilization of biosurfactant produced by Pseudomonas aeruginosa strain LTR1 for the synthesis of the silver nanoparticles and their application as antimicrobial agents","authors":"D. Nitin, K. Dilip, D. Pravin, Kathwate Gunderao","doi":"10.7324/jabb.2022.100205","DOIUrl":"https://doi.org/10.7324/jabb.2022.100205","url":null,"abstract":"Nitin Deshmukh1, Dilip Kadam2, Pravin Deshmukh3, Gunderao Kathwate4* 1Department of Microbiology, K. N. Bhise Arts, Commerce and Vinayakrao Patil Science College, Bhosare, Solapur, India. 2Department of Microbiology, D. B. F. Dayanand Arts and Science College, Solapur, India. 3Department of Microbiology, Pratapsinh Mohite Patil Mahavidyalaya, Karmala, India. 4Department of Biotechnology, Savitribai Phule Pune University, Pune, India.","PeriodicalId":15032,"journal":{"name":"Journal of Applied Biology and Biotechnology","volume":"12 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-02-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"85619128","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Interaction efficiency of Trichoderma spp. and some plant extracts against ear-cockle disease","authors":"Al-Taie Azher Hameed Faraj, Al-Zubaid Noor Kadhum","doi":"10.7324/jabb.2022.100213","DOIUrl":"https://doi.org/10.7324/jabb.2022.100213","url":null,"abstract":"Ear-cockle disease is one of the ‎important aerial diseases of wheat. It is caused by wheat seed gall nematode Anguina tritici. This study aimed to evaluate the interaction effect between Trichoderma spp. as bioagents and some plant extracts. The latter are used as alternative substances to ‎control the seed gall nematode in both laboratory and field conditions. The bioassay of juveniles in the second stage (J2) of A. tritici was‎ conducted using the biopesticide Biocont-T-WP (Trichoderma ‎harzianum), isolate of Trichoderma hamatum T-113, and ‎nematic and seaweed extract for their effect on the viability of A. tritici J2. nematicide Rugby 100 has been used as a positive control.‎ The lab-bioassay was achieved on the daily accumulated J2 mortality percentage during ‎‎1, 3, 5, 7, 9, and 11 days of incubation, while the field-bioassay was evaluated for‎ wheat growth, yield, and infection attributes.‎ The lab-bioassay showed that ‎Biocont-T has a higher mortality effect followed by the nematic extract with averages of 40.56% and ‎‎12.52%, respectively. The accumulative J2 mortality percentage increased gradually and reached a maximum on the 11th day of incubation. ‎In the field-bioassay, mixing wheat ‎seed Ibaa-99 with Biocont-T decreased infection percentage 77.7% and the number of galls spike–1 to 1.63 compared to the control 5.66 galls spike–1.","PeriodicalId":15032,"journal":{"name":"Journal of Applied Biology and Biotechnology","volume":"220 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-02-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"75732293","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}