Journal of Applied Biology and Biotechnology最新文献

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Beneficial effects of soaking and germination on nutritional quality and bioactive compounds of biofortified wheat derivatives 浸泡和萌发对生物强化小麦衍生物营养品质和活性成分的有益影响
Journal of Applied Biology and Biotechnology Pub Date : 2021-10-01 DOI: 10.7324/jabb.2021.9503
V. Meena, Kumar Vinod, S. Imran, Sangwan Punesh, Bora Roop Singh, Yadav Ajar Nath, Dhaliwal Harcharan Singh
{"title":"Beneficial effects of soaking and germination on nutritional quality and bioactive compounds of biofortified wheat derivatives","authors":"V. Meena, Kumar Vinod, S. Imran, Sangwan Punesh, Bora Roop Singh, Yadav Ajar Nath, Dhaliwal Harcharan Singh","doi":"10.7324/jabb.2021.9503","DOIUrl":"https://doi.org/10.7324/jabb.2021.9503","url":null,"abstract":"Meena Verma1, Vinod Kumar2, Imran Sheikh1, Punesh Sangwan3, Roop Singh Bora1*, Ajar Nath Yadav1, Harcharan Singh Dhaliwal1 1Department of Genetics, Plant Breeding and Biotechnology, Khem Singh Gill Akal College of Agriculture, Eternal University, Baru Sahib, India. 2Department of Biochemistry, CCS Haryana Agricultural University, Hisar, India. 3Akal College of Basic Sciences, Eternal University, Baru Sahib, India.","PeriodicalId":15032,"journal":{"name":"Journal of Applied Biology and Biotechnology","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46207381","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Seasonal analysis of certain biochemical parameters of carps cultured in domestic sewage oxidation ponds 生活污水氧化池养殖鲤鱼某些生化参数的季节分析
Journal of Applied Biology and Biotechnology Pub Date : 2021-10-01 DOI: 10.7324/jabb.2021.9520
Ali Sharique A., Raju Hanumanth, Parveen Naima
{"title":"Seasonal analysis of certain biochemical parameters of carps cultured in domestic sewage oxidation ponds","authors":"Ali Sharique A., Raju Hanumanth, Parveen Naima","doi":"10.7324/jabb.2021.9520","DOIUrl":"https://doi.org/10.7324/jabb.2021.9520","url":null,"abstract":"Aquaculture from domestic sewage water is an alternate solution for growing scarcity of food to the pressures of population by producing fish and prawn using treated domestic waste effluents. It is an economically viable process particularly in the developing countries, including West Bengal, India. Keeping in view of the above viability, we have cultured three species of fishes Cyprinus carpio, Labeo rohita, and Cirrhinus mrigala in domestic sewage oxidation ponds designed as per National Environmental Engineering Research Institute, India. Biochemical parameters such as serum lactate and serum cholesterol have been investigated in the present study to determine the quality of fishes cultured in sewage oxidation ponds for human consumption. Among all experimental fishes, C. carpio exhibited higher serum lactate (360.40 μg/ml) as compared to L. rohita (301.43 μg/ml) and C. mrigala (286.73 μg/ml). Similarly, high serum cholesterol values were observed in C. carpio as compared to other two species in all the three seasons. During the monsoon season, maximum serum cholesterol were observed in sewage cultured C. carpio (430.45 mg/100 ml) and minimum (278.40 mg/ml) in summer season followed by L. rohita (248.25 and 198.21 mg/100 ml) and C. mrigala (278.40 and 238.37 mg/100 ml). The fishes cultured in fresh water control ponds showed comparatively low values of serum lactate and cholesterol than the experimental fishes. Our results support the concept that good survival and adjustment of the fishes to the pre-treated nutritive domestic sewage water leads to their significant growth with an increased biochemical profile especially with higher levels of cholesterol, which are season dependent.","PeriodicalId":15032,"journal":{"name":"Journal of Applied Biology and Biotechnology","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44839534","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 2
Rice crop loss due to major pathogens and the potential of endophytic microbes for their control and management 主要病原菌造成的水稻作物损失及其内生微生物控制和管理的潜力
Journal of Applied Biology and Biotechnology Pub Date : 2021-10-01 DOI: 10.7324/jabb.2021.9523
Nayak Shubhransu, Samanta Soma, Sengupta Chandan, Swain Soumya Sephalika
{"title":"Rice crop loss due to major pathogens and the potential of endophytic microbes for their control and management","authors":"Nayak Shubhransu, Samanta Soma, Sengupta Chandan, Swain Soumya Sephalika","doi":"10.7324/jabb.2021.9523","DOIUrl":"https://doi.org/10.7324/jabb.2021.9523","url":null,"abstract":"Millions of people around the world depend on rice as the staple food which is infested by many pathogens causing a huge loss. Synthetic chemicals, fungicides, and bactericides are being used massively to control these pathogens in many countries. Although these pesticides are being able to control many pathogens, non-judicious applications may lead to many environmental and health concerns. Utilization of endophytic microorganisms may be an eco-friendly and sustainable approach in this direction. Endophytic microorganisms remain asymptomatically inside the plants in a symbiotic manner and impart resistance to plants from many biotic and abiotic stresses. Many endophytes have proved to have antagonistic effects toward many pathogens of plants. Some potential endophytes have consistently been isolated from rice and other plants which could control the growth of many rice pathogens. Considering the importance of rice and its many pathogen enemies, research on the use of endophytes to control these pathogens needs to be intensified to minimize crop loss and to meet future rice demands. The present review accentuated the potential of endophytic microorganisms to control some of the important rice pathogens which cause huge loss in many rice-growing areas of the world. This review may encourage researchers for intensified and integrative research in the mentioned area.","PeriodicalId":15032,"journal":{"name":"Journal of Applied Biology and Biotechnology","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45450101","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 5
Dengue infection profile in Gujarat, West India: A recent report 西印度古吉拉特邦登革热感染概况:最近的一份报告
Journal of Applied Biology and Biotechnology Pub Date : 2021-10-01 DOI: 10.7324/jabb.2021.9514
Naik Nishita S., Murarka Shiva V., S. Dhariya, Shah Bhavini S., Raval Rutvik J., Rao Mandava V.
{"title":"Dengue infection profile in Gujarat, West India: A recent report","authors":"Naik Nishita S., Murarka Shiva V., S. Dhariya, Shah Bhavini S., Raval Rutvik J., Rao Mandava V.","doi":"10.7324/jabb.2021.9514","DOIUrl":"https://doi.org/10.7324/jabb.2021.9514","url":null,"abstract":"Dengue fever (DF) is a mosquito-borne viral disease occurring in tropical and subtropical areas including India. It is manifested by symptoms like fever, headache, rashes, muscle, and joint pains. In our country, Gujarat is one of the highest infected states by this disease due to mosquito bites. Blood samples from such cases were collected and assessed for blood counts, serum transaminases, and blood smears from only positive patients using standard protocols. Real-time PCR (RT-PCR) technique was also employed for virus detection. Of 1020 referral cases, from Ahmedabad, Vadodara, Jamnagar, and Anand districts, 184 cases were detected positive (18.03%) from October 2016 to December 2019 (39 months) being highest in 2019. Males had a high frequency (52%) of infection when compared to females (48%), affecting young age groups ranging from moderate to higher level. Furthermore, Ahmedabad district (57%) had a high percentage of infection, compared to others. Marked alterations were also observed with respect to serum glutamate-pyruvate transaminase and serum glutamic oxaloacetic transaminase, platelets, lymphocytes, and WBC counts in positive cases of 0–20, 21–40, and 40–50 year age groups. Reduced platelet clumping and severe thrombocytopenia (59%) in these cases are early diagnostic markers of dengue, supporting RT-PCR method. Thus from our study, we conclude that dengue infection is caused by various factors such as sex, climate, population, and environment. This outbreak is hence controlled by following health measures strictly, imposed by health departments in Gujarat, Western India.","PeriodicalId":15032,"journal":{"name":"Journal of Applied Biology and Biotechnology","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47780460","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 1
Prevalence of malaria and typhoid fever and coinfection in all febrile patients attending at tertiary care hospital in Vadodara 在瓦多达拉三级保健医院就诊的所有发热病人的疟疾和伤寒患病率及合并感染情况
Journal of Applied Biology and Biotechnology Pub Date : 2021-10-01 DOI: 10.7324/jabb.2021.9518
Vasava Sangita Devubhai, Lakhani Sucheta Jitendra, Lakhani Jitendra Devjibhai
{"title":"Prevalence of malaria and typhoid fever and coinfection in all febrile patients attending at tertiary care hospital in Vadodara","authors":"Vasava Sangita Devubhai, Lakhani Sucheta Jitendra, Lakhani Jitendra Devjibhai","doi":"10.7324/jabb.2021.9518","DOIUrl":"https://doi.org/10.7324/jabb.2021.9518","url":null,"abstract":"Malaria and typhoid fevers are common disorders of tropical countries, which can lead to substantial morbidity and mortality. It is an important cause of fever in India. The prevalence of malaria, typhoid, and their coinfection was studied at a tertiary care, rural-based teaching hospital. A total of 1,378 blood samples from febrile patients in whom initial diagnosis was of either malaria or typhoid fever were analyzed at the Department of Microbiology, SBKS Medical Institute and Research Centre, Pipalia, (District: Vadodara), India. The blood samples received from the outpatient department and also from the inpatient department of all age groups during January 2015–December 2019 were analyzed. The rapid diagnostic test and peripheral smear by Giemsa stain was carried out for diagnosis of malaria. Widal test was used for screening for typhoid which was further confirmed by blood culture and clinical diagnosis. Of the 1,378 febrile patients, 271 and 95 cases were diagnosed with typhoid fever and malaria, respectively. Coinfection was found in 18 (4.7%) cases. Of the total 1,378 febrile patients initially screened for both these conditions, males and females were 50.7% and 49.3%, respectively. Of the 95 patients of malaria, male preponderance was present (72.6% vs 27.4%), while typhoid had female dominance (male: 45.4%; female: 54.6%). The prevalence of malaria, typhoid, and coinfection was highest in the age group of 16–30 years, which was around 43%–45%. The study revealed that the typhoid prevalence rate was higher than malaria in patients in whom initial diagnosis was made of either malaria or typhoid. Fever cases, in which either malaria or typhoid was suspected on basis of clinical symptoms (27.86%, 384 out of 1,378 samples), were having positive results of either malaria, typhoid, or both.","PeriodicalId":15032,"journal":{"name":"Journal of Applied Biology and Biotechnology","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44703636","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Effects of various factors on biomass, bioethanol, and biohydrogen production in green alga Chlamydomonas reinhardtii 不同因素对莱茵衣藻生物量、生物乙醇和生物氢产量的影响
Journal of Applied Biology and Biotechnology Pub Date : 2021-10-01 DOI: 10.7324/jabb.2021.9521
Sudha Narravula Raga, Varaprasad Duddela, Bramhachari Pallaval Veera, S. Poda, Chandrasekhar Thummala
{"title":"Effects of various factors on biomass, bioethanol, and biohydrogen production in green alga Chlamydomonas reinhardtii","authors":"Sudha Narravula Raga, Varaprasad Duddela, Bramhachari Pallaval Veera, S. Poda, Chandrasekhar Thummala","doi":"10.7324/jabb.2021.9521","DOIUrl":"https://doi.org/10.7324/jabb.2021.9521","url":null,"abstract":"Extensive usage of nonrenewable fossil fuels has urged the community to look into alternate renewable energy such as biofuels. The production of biofuels, including bioethanol, biohydrogen, biodiesel, etc., from crop plants again leads to food insecurity. Hence, the usage of algae, particularly green algae, is the best alternatives to generate biofuels. In the present study, the effects of various factors involved in algal growth in turn bioethanol and biohydrogen production levels were established by choosing green alga Chlamydomonas reinhardtii. Specifically, we crosschecked the effect of heavy metal Cobalt (Co), sodium bicarbonate, and Tris-acetate-phosphate (TAP) medium without Tris base on growth and biofuel production from C. reinhardtii under in vitro conditions. In the current work, 0.2 mg/l Co in TAP medium enhanced the growth and biomass in C. reinhardtii cultures. Furthermore, ethanol production levels were improved in feedstocks grown in TAP medium with 2.0 mg/l sodium bicarbonate. In another experiment, hydrogen production was high in full TAP medium with Tris (trisaminomethane) base when compared to TAP without Tris base. In conclusion, this work may be useful to improve the biomass and biofuel production in green algae.","PeriodicalId":15032,"journal":{"name":"Journal of Applied Biology and Biotechnology","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43187218","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 2
Morphological status of multipolar neurons in the lateral cortical area of the telencephalon of a catfish, Clarias batrachus: A Golgi study 鲶鱼端脑外侧皮层多极神经元的形态学状态:高尔基体的研究
Journal of Applied Biology and Biotechnology Pub Date : 2021-10-01 DOI: 10.7324/jabb.2021.9511
Mohanty Banalata, Ojha Anil Kumar
{"title":"Morphological status of multipolar neurons in the lateral cortical area of the telencephalon of a catfish, Clarias batrachus: A Golgi study","authors":"Mohanty Banalata, Ojha Anil Kumar","doi":"10.7324/jabb.2021.9511","DOIUrl":"https://doi.org/10.7324/jabb.2021.9511","url":null,"abstract":"The present study explored the morphological features of multipolar neurons in the lateral cortical area of the telencephalon of a catfish, Clarias batrachus, employing Golgi staining. In C. batrachus, the multipolar neurons have round to multiangular soma with axon emerged adjacent to either basal or apical dendrites. Branched apical and basal dendrites are of various thicknesses with few dendritic spines on them. Multipolar neurons have shown uniform distribution in both the center and toward the boundary of the lateral cortical area. The morphological features and position suggest that the role of this neuronal type in maintaining the local circuit as well as in transmitting information acting as projection neurons to the adjacent telencephalic region.","PeriodicalId":15032,"journal":{"name":"Journal of Applied Biology and Biotechnology","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42304612","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
A comprehensive study of microbiological profile, risk factors and antibiotic sensitivity pattern of catheter associated urinary tract infection in a teaching hospital of Gujarat 古吉拉特邦一家教学医院导管相关性尿路感染的微生物学特征、危险因素和抗生素敏感性模式的综合研究
Journal of Applied Biology and Biotechnology Pub Date : 2021-10-01 DOI: 10.7324/jabb.2021.9512
Panjwani Dipak Motilal, Lakhani Sucheta Jitendra, Mehta Sanjay Jayantilal, Kikani Kunjan Madhukar, Shah Khushi Shyam
{"title":"A comprehensive study of microbiological profile, risk factors and antibiotic sensitivity pattern of catheter associated urinary tract infection in a teaching hospital of Gujarat","authors":"Panjwani Dipak Motilal, Lakhani Sucheta Jitendra, Mehta Sanjay Jayantilal, Kikani Kunjan Madhukar, Shah Khushi Shyam","doi":"10.7324/jabb.2021.9512","DOIUrl":"https://doi.org/10.7324/jabb.2021.9512","url":null,"abstract":"The current study was carried out to find the microbiological profile along with the risk factors and antibiotic sensitivity patterns of catheter-associated urinary tract infection in a teaching hospital. All the catheterized patients satisfying the criteria of catheter-associated urinary infection (CAUTI) and catheter-associated asymptomatic bacteriuria (CA-ASB) were included in the study. Urine samples received from CAUTI patients were followed-up further. Samples were collected and inoculated on MacConkey’s agar and Blood agar. Identification and antibiotic sensitivity patterns of bacterial isolates were carried out by automated system, i.e., Vitek-2, as per standard protocols. Duration of catheterization and various risk factors were also recorded in detail. Out of 247 urine samples of hospital acquired infection, 50 samples satisfied the criteria of CAUTI. The most common organism isolated was Escherichia coli (38%), followed by Pseudomonas sp. (24%) and Klebsiella sp. (24%). Maximum isolates were from the medicine ward (40%). Gram-negative isolates showed dominance over the Gram-positive isolates. Such increasing figures of CAUTI incidents pose a great challenge to the hospital management and threat to the patients with underlying illness. With strict adherence to the catheter care of patients and use of infection control practices, we can significantly decline the rate of developing CAUTI.","PeriodicalId":15032,"journal":{"name":"Journal of Applied Biology and Biotechnology","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48228880","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Correlates of sperm quality parameters and oxidative stress indices in diabetic rats exposed to cold stress: Role of Moringa oleifera leaf extract 辣木叶提取物对糖尿病大鼠冷应激精子质量参数和氧化应激指数的影响
Journal of Applied Biology and Biotechnology Pub Date : 2021-10-01 DOI: 10.7324/jabb.2021.9510
Basha Piler Mahaboob, Rakesh Hanumanthappa, Mani Saumya S.
{"title":"Correlates of sperm quality parameters and oxidative stress indices in diabetic rats exposed to cold stress: Role of Moringa oleifera leaf extract","authors":"Basha Piler Mahaboob, Rakesh Hanumanthappa, Mani Saumya S.","doi":"10.7324/jabb.2021.9510","DOIUrl":"https://doi.org/10.7324/jabb.2021.9510","url":null,"abstract":"Spermatogenesis is extremely sensitive to fluctuations in the environment, particularly temperature and hormones. Sperm dysfunction, a root cause of male infertility, is a commonly allied complication of diabetes mellitus. Our previous studies cogitate that cold stress (15°C) exacerbates the complications and the resultant oxidative stress plays a major role in testicular and epididymis dysfunction in diabetic rats. Despite the strong biologic prospect for this postulation, establishing a direct link between free radicals and specific disease is an in-dire need, and in this context, this study focuses on investigating the sperm quality parameters and their relationship to testicular oxidative stress indices of cold stress diabetes in Wistar rats. The results indicate a cumulative impact by diminishing sperm parameters, viz. sperm density, viability, motility, mortality, and acrosome intactness in cold-stressed diabetic rats. The findings also reveal a strong positive Pearson’s correlation between the sperm quality parameters and testicular lipid peroxidation, which reflects the influence of oxidative stress on sperm dysfunction. Together with duel stressor effects, the efficacy of Moringa oleifera leaf ethanolic (MOLE) extract is appended to assess its therapeutic role. The apparent effectiveness of MOLE therapy at 250 and 500 mg/kg bw for 60 days aided in suppressing oxidative stress and improved semen quality demonstrating the causative nature of these associations; hence, Moringa usage is recommended as a therapeutic agent for male reproductive dysfunctions in population residing in colder climates.","PeriodicalId":15032,"journal":{"name":"Journal of Applied Biology and Biotechnology","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46825400","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 1
Study of pathogenic traits of bacterial wilt-causing phytopathogens around Kanpur and Fatehpur regions, Uttar Pradesh, India 印度北方邦坎普尔和法特赫布尔地区引起青萎病的植物病原体的致病特性研究
Journal of Applied Biology and Biotechnology Pub Date : 2021-10-01 DOI: 10.7324/jabb.2021.9507
Umrao Pramila Devi, K. Vineet, Kaistha Shilpa Deshpande
{"title":"Study of pathogenic traits of bacterial wilt-causing phytopathogens around Kanpur and Fatehpur regions, Uttar Pradesh, India","authors":"Umrao Pramila Devi, K. Vineet, Kaistha Shilpa Deshpande","doi":"10.7324/jabb.2021.9507","DOIUrl":"https://doi.org/10.7324/jabb.2021.9507","url":null,"abstract":"Bacterial wilt is a major cause of concern in many economically essential solanaceous crops present in tropical, subtropical, and some moderate areas of the world. Ralstonia solanacearum is represented as a dominant bacterial wilt-causing phytopathogen in most studies. However, there is not much information regarding bacterial wilt-causing bacterial phytopathogens around Kanpur and Fatehpur districts of Uttar Pradesh, India. Samples were collected from ooze leaking off from stem, vascular component of wilted tomato plants, and inflamed potato tuber and isolated on triphenyl tetrazolium chloride medium. 22 out of initial 57 isolates were identified as R. solanacearum by morphological, physiological, biochemical, and serological tests. All isolates showed pathogenicity in tomato seedlings and potato tuber inoculation. The characterization of pathogenic traits such as biofilm formation, antibiotic resistance, siderophore and hydrogen cyanide production, potassium solubilization and hydrolytic enzymes, such as cellulase, amylase, gelatinase, and pectinase has been reported. 16S ribosomal ribonucleic acid sequencing showed sequence similarity in NCBI BLAST analysis to members of Enterobacter cloaceae, Serratia marcescens, and Providencia vermicola. Hence, a larger sample study and further genome level characterization is required to understand the biodiversity of bacterial wilt-causing pathogens around Uttar Pradesh, India.","PeriodicalId":15032,"journal":{"name":"Journal of Applied Biology and Biotechnology","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43118133","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
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