Journal of andrology最新文献

{"title":"Amelioration of Sperm Agglutinating Factor (SAF) Induced Sperm Impairment by Anti-SAF Polyclonal Antibody","authors":"K. Kaur, P. Rishi, V. Prabha","doi":"10.4172/2167-0250.1000109","DOIUrl":"https://doi.org/10.4172/2167-0250.1000109","url":null,"abstract":"Background: We have previously isolated a Spermagglutinating Factor (SAF) from Escherichia coli, which was capable of causing sperm agglutination and impairment of sperm parameters viz. apoptosis, premature acrosome loss and inhibition of Mg2+ dependent ATPase activity invitro. In addition, intravaginal administration of Balb/c mice with SAF resulted in infertility. To provide evidence that SAF plays an important role in impairment of sperm parameters and infertility, anti-SAF antiserum was raised and its application as a therapeutic intervention against SAF induced damage was evaluated. \u0000Methods: Effect of anti-SAF antiserum was evaluated against SAF mediated adverse effects on sperm parameters. Sperm agglutination was observed using light microscopy and Mg2+ dependent ATPase activity was estimated in terms of release of inorganic phosphate. Sperm apoptosis and acrosome status were evaluated by means of flow cytometery and fluorescent microscopy, respectively. Further, the impact of anti-SAF antiserum was also seen on fertility outcome in mice \u0000Results: Results showed that immunization of mice with SAF lead to the generation of high titer specific antibodies. Raised anti-SAF antiserum could neutralize all the biological effects of SAF in contrast to control antiserum. Furthermore, intravaginal application of anti-SAF antiserum along with SAF rendered mice fertile. \u0000Conclusion: Here we provide evidence that SAF plays an imperative role as all the detrimental effects induced by SAF whether in vitro or in vivo were blocked on simultaneous incubation with anti-SAF antiserum. Present work also highlighted the efficacy of the anti-SAF antiserum as a curative measure against SAF.","PeriodicalId":15029,"journal":{"name":"Journal of andrology","volume":"2 1","pages":"1-6"},"PeriodicalIF":0.0,"publicationDate":"2013-11-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"70783691","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The Effects of Short Abstinence Time on Sperm Motility, Morphology and DNA mDamage","authors":"M. Sukprasert, A. Wongkularb, S. Rattanasiri, W. Choktanasiri, C. Satirapod","doi":"10.4172/2167-0250.1000107","DOIUrl":"https://doi.org/10.4172/2167-0250.1000107","url":null,"abstract":"This was an experimental study to determine the influence of short abstinence time on sperm motility, morphology and DNA damage performed at the Assisted Reproductive Technology (ART) unit, Ramathibodi Hospital. Fifty-seven semen samples with normal semen analysis according to World Health Organization (WHO) 1999 and Kruger strict criteria were obtained. Volunteers were instructed to abstain for 3-5 days, 18-30 hours and 4 days for the 1st, 2nd and 3rd semen collection Total motile sperm count (TMSC) was determined using computer-assisted semen analysis (CASA); sperm morphology was determined using eosin-methylene blue staining and DNA damage was assessed by TUNEL assay. The results showed that TMSC was not significantly different between the 1st (3-5 days abstinence time) and 2nd semen collection (18-30 hours abstinence time after the 1st collection) (p value=0.289). The percentage of DNA damage (4.6% vs. 9.8%, p value<0.001) and normal morphological sperm (14.9% vs. 17.2%, p value<0.001) in the 2nd semen collection were significantly lower than the 1st collection, although the value was still in the normal range. Semen parameters of the 3rd collection (4 days abstinence time after the 2nd collection) correlate with those of the 1st collection. We conclude that in normal semen, with an abstinence time of only one day, TMSC was not significantly different between 3-5 days abstinence time and 18-30 hours abstinence time. The result of DNA damage and the percentage of normal morphological sperm were significantly lower in 18-30 hours abstinence time.","PeriodicalId":15029,"journal":{"name":"Journal of andrology","volume":"2 1","pages":"1-5"},"PeriodicalIF":0.0,"publicationDate":"2013-10-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"70783472","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"If Nature Failed Creating the Perfect Prostate Could Inhibitors ofProteolysis Help","authors":"J. Jankun","doi":"10.4172/2167-0250.1000E112","DOIUrl":"https://doi.org/10.4172/2167-0250.1000E112","url":null,"abstract":"In young man it is working flawlessly and begins deteriorating in the forties, fifties or sixties when some men start to have problems with urinating, a benign prostate hyperplasia (BPH), hematuria and worries about prostate cancer. Rates of diagnosis of prostate cancers is different across the world, with lowest in developing countries, especially in Africa, followed by South and East Asia where it is detected much less frequently than in Europe and in the United States. However, mortality rates are the highest in the developing countries [1-3]. Clearly, advanced prostate cancer treatment available in the medically advanced communities prolongs overall survival in men. For example, decline in prostate cancer mortality in the USA compared with the UK in 19942004 coincided with more frequent PSA screening in the USA and more aggressive treatment of the disease [4]. Nevertheless, National cancer Institute (NCI) on its home page estimates that in 2013 it will be approximately 240,000 new cases of prostate cancer and 30,000 deaths related to this disease [5]. Considering that over 30% of men over the age of fifty have histological evidence of prostate cancer on biopsy and that this percentage increases with age, it seems that mortality rate of prostate cancer is not very high in the USA. So it might be that prostate is not such bad organ after all. However, this disease is unpredictable resulting in the mortality as the consequence of the distant metastases [6] most notably, but not exclusively to the bones. Thus it is not a surprise that NCI lists over 460 clinical studies in prostate cancer. They include predominantly androgen deprivation therapy, radiation and others concentrating mainly on reducing cancer cell growth or cancer cell killing. It raises the question if it is any room for different and novel approach for treatment of prostate cancer and its advanced forms.","PeriodicalId":15029,"journal":{"name":"Journal of andrology","volume":"2 1","pages":"1-2"},"PeriodicalIF":0.0,"publicationDate":"2013-08-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"70784930","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Seminal Parameters: Current Status","authors":"P. F. Taitson, Eduardo Yoneyama Mourthé","doi":"10.4172/2167-0250.1000E111","DOIUrl":"https://doi.org/10.4172/2167-0250.1000E111","url":null,"abstract":"The World Health Organization laboratory manual for the examination of human semen and sperm–cervical mucus interaction was first published in 1980, in response to a growing need for the standardization of procedures for the examination of human semen. It has since been updated three times, and translated into a number of languages. Over the past 30 years, the manual has been recognized as providing global standards and has been used extensively by research and clinical laboratories throughout the world. Classically, infertility is defined as the inability to achieve a clinical pregnancy after 12 months or more of regular unprotected sexual intercourse. The incidence of male infertility is the same woman, i.e. around 40%. The other 20% apply to both. On the other hand, the chance of a couple to become pregnant is not high. Is around 15-17% per month [1].","PeriodicalId":15029,"journal":{"name":"Journal of andrology","volume":"2 1","pages":"1-2"},"PeriodicalIF":0.0,"publicationDate":"2013-07-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"70785277","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Evaluation of Age Effects on Semen Parameters of Infertile Males","authors":"H. Omran, M. Bakhiet, M. Dashti","doi":"10.4172/2167-0250.1000106","DOIUrl":"https://doi.org/10.4172/2167-0250.1000106","url":null,"abstract":"Background: The contributions of host factors, such as age, in male infertility are still poorly understood. Thus, the present work has aimed to evaluate the effect of patient’s age on semen parameters of infertile males. \u0000Method: Semen samples from 52 infertile patients aged 21-52 years (mean 30.8 ± 6.7) were analysed by conventional semen analysis methods, flowcytometry analysis for sperm DNA integrity, and colorimetric assay for total antioxidant capacity. \u0000Results: The study revealed that, as patient’s age increases, there was a significant reductionin sperm density, motility, percentage of morphologically normal spermatozoa, total antioxidant capacity and DNA integrity. \u0000Conclusion: The study results demonstrated the negative influence of increasing patient’s age on semen parameters; and the possible role of patient’s age in the aetiology of male infertility.","PeriodicalId":15029,"journal":{"name":"Journal of andrology","volume":"2 1","pages":"1-5"},"PeriodicalIF":0.0,"publicationDate":"2013-06-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"70783817","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Reproductive Hormone Profiles during Imatinib Therapy in Men with Chronic Myeloid Leukemia","authors":"K. Bay, O. Bjerrum, U. Olsson‐Strömberg, K. Porkka, I. Dufva, A. Andersson","doi":"10.4172/2167-0250.1000105","DOIUrl":"https://doi.org/10.4172/2167-0250.1000105","url":null,"abstract":"Imatinib side effects related to testicular function have been reported in male patients with chronic myeloid leukemia. These include decreased testosterone levels, gynecomastia and impaired spermatogenesis. To further investigate testicular function in relation to imatinib treatment, a longitudinal study on reproductive hormone profiles was conducted in 17 male patients with chronic myeloid leukemia. Blood samples were taken before and at one or more time points during imatinib therapy. Serum samples were analyzed for the hormones testosterone, estradiol, and luteinizing hormone (LH) to reflect testicular Leydig cell function. Sex hormone-binding globulin (SHBG) serum levels were measured to evaluate free testosterone, and serum levels of inhibin B and follicle stimulating hormone were measured to reflect spermatogenesis. Out of the 17 patients included in the study, one patient developed gynecomastia after 7-10 months of therapy. Testosterone levels were generally low in the patients both before and during the study, and did not change in response to imatinib therapy. Conversely, SHBG levels decreased transiently at 3 and 6-9 months of therapy (p=0.002 and p=0.008, respectively). Estradiol levels decreased at 12-15 months of therapy (p=0.048). LH and hormones reflecting spermatogenesis were unchanged. In conclusion, our longitudinal study of men with chronic myeloid leukemia showed a significant, but largely transient, decrease in SHBG levels in response to imatinib therapy. Testosterone levels were low in the men both before and during imatinib therapy.","PeriodicalId":15029,"journal":{"name":"Journal of andrology","volume":"2 1","pages":"1-6"},"PeriodicalIF":0.0,"publicationDate":"2013-05-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"70783760","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"DISSECTING ANDROGEN ACTION: NEW CLUES FROM CONDITIONAL KNOCKOUT MICE","authors":"Lee B. Smith","doi":"10.1530/ENDOABS.32.S26.2","DOIUrl":"https://doi.org/10.1530/ENDOABS.32.S26.2","url":null,"abstract":"","PeriodicalId":15029,"journal":{"name":"Journal of andrology","volume":"33 1","pages":"31-31"},"PeriodicalIF":0.0,"publicationDate":"2013-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"67269001","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comparative Study of Four Different Sperm Washing Methods Using Apoptotic Markers in Ram Spermatozoa","authors":"Elena Martí,&nbsp;Rosaura Pérez-Pé,&nbsp;Teresa Muiño-Blanco,&nbsp;José A. Cebrián-Pérez","doi":"10.2164/jandrol.106.000109","DOIUrl":"10.2164/jandrol.106.000109","url":null,"abstract":"<p><b>ABSTRACT: </b> The accurate measurement of semen fertilizing potential is of great importance in determining the acceptability of processed semen for breeding purposes. A good sperm preparation technique results in a sample with high viability and motility and also takes into account other parameters such as the capacitation and apoptotic state which could compromise the ability to fertilize an oocyte. In this study, we investigate the effects of 4 sperm preparation techniques (a dextran/swim-up procedure, discontinuous Percoll density gradient centrifugation, sucrose washing, and filtration) on ram sperm quality parameters. Besides the evaluation of viability and the capacitation state, we also analyzed the apoptotic status of the sperm samples by assessing the phosphatidylserine translocation and caspase-3 and −7 activities. This is the first report, to our knowledge, that evidences the presence of active caspases in ram sperm. The results confirm the better ability of the dextran/swim-up procedure to select nonapoptotic spermatozoa, in addition to viable and noncapacitated sperm, compared with other sperm preparation methods. This should be considered to enhance results of artificial insemination techniques in ovine reproduction.</p>","PeriodicalId":15029,"journal":{"name":"Journal of andrology","volume":"27 6","pages":"746-753"},"PeriodicalIF":0.0,"publicationDate":"2013-01-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.2164/jandrol.106.000109","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"26117289","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Ejaculate Volume Is Seriously Underestimated When Semen Is Pipetted or Decanted Into Cylinders From the Collection Vessel","authors":"Trevor G. Cooper,&nbsp;Charlene Brazil,&nbsp;Shanna H. Swan,&nbsp;James W. Overstreet","doi":"10.2164/jandrol.106.001297","DOIUrl":"10.2164/jandrol.106.001297","url":null,"abstract":"<p>The total sperm count (number of spermatozoa per ejaculate) rather than sperm concentration (number of spermatozoa per unit volume of semen) is the more important semen variable related to fertility. It reflects testicular volume (Handelsman et al, 1984; Andersen et al, 2000; Behre et al, 2000), and thus is a measure of total testicular sperm output (MacLeod and Wang, 1979), which is directly related to the chances of pregnancy after coitus. The concentration of spermatozoa in the ejaculate, however, depends on the extent of dilution of epididymal spermatozoa by secretions of the prostate and seminal vesicles occurring at ejaculation and is therefore influenced by the secretory capacity of the accessory sex glands. This is an important distinction, for when comparing semen quality from older and younger men, sperm concentrations do not differ, yet semen volume is reduced in the older men, and so the total number of spermatozoa per ejaculate is lower in the older men (Ng et al, 2004; Nieschlag et al, 1982). The total number of spermatozoa per ejaculate is obtained by multiplying the concentration of spermatozoa by the semen volume. The latter is best measured by weighing (Eliasson, 2003), assuming a density of 1.0 g/mL (Auger et al, 1995; Jorgensen et al, 1997, 2001; Brazil et al, 2004), but alternative methods, such as collection into graduated cylinders (Behre et al, 2000), pipetting from the collection vessel (Mortimer 1994; Jorgensen et al, 1997), and pouring from the collection vessel into a graduated tube (Jorgensen et al, 1997), are in current practice.</p><p>Two recent studies have found that pipetting semen from the collection vessel leads to an underestimation of about 0.5 mL (range 0.3–0.8 mL; Brazil et al, 2004; Iwamoto et al, 2006) compared with weighing, but no data are available about losses incurred when pouring semen into graduated cylinders. Because the area of contact with the sides of the collection vessel while decanting semen into a graduated cylinder is likely to be far larger than that during pipetting, retention within the vessel could be much larger, leading to a larger underestimation of volume with this method. In this study, new data are obtained on the loss of semen volume during decanting to a cylinder and previously published results on losses because of pipetting, and the density of semen is reanalyzed together with additional data.</p><p>This study has shown that a consistent and significant reduction in the volume of semen is obtained when a pipette or a graduated cylinder is used to measure liquefied semen transferred from its collection vessel. These losses cannot be accounted for by evaporation because samples were capped during liquefaction at room temperature and pipetted or decanted immediately after weighing. It could be that with particularly viscous samples, transfer would result in even lower volumes because more would be retained on the side of the decanting vessel and some might adhere to the sides","PeriodicalId":15029,"journal":{"name":"Journal of andrology","volume":"28 1","pages":"1-4"},"PeriodicalIF":0.0,"publicationDate":"2013-01-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.2164/jandrol.106.001297","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"26240760","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Acrosome Reaction Induced by Recombinant Human Zona Pellucida 3 Peptides rhuZP3a22∼176 and rhuZP3b177∼348 and Their Mechanism","authors":"Ya Ni,&nbsp;Kun Li,&nbsp;Wanxiang Xu,&nbsp;Liwen Song,&nbsp;Kangshou Yao,&nbsp;Xinzong Zhang,&nbsp;Hefeng Huang,&nbsp;Yanling Zhang,&nbsp;Qi-Xian Shi","doi":"10.2164/jandrol.106.001289","DOIUrl":"10.2164/jandrol.106.001289","url":null,"abstract":"<p><b>ABSTRACT: </b> To explore the biological characteristics of the recombinant zona pellucida 3 (ZP3) peptides of rhuZP3a^22∼176 and rhuZP3b^177∼348, we examined whether rhuZP3a^22∼176 or rhuZP3b^177∼348 trigger the acrosome reaction (AR) of human spermatozoa and we investigated the underlying mechanism. The assessment of AR was performed using chlortetracycline staining. The intracellular free calcium concentration ([Ca²⁺]i) in Fura-2/AM-loaded human sperm was monitored with a spectrofluorophotometer. We found that rhuZP3a^22∼176 and rhuZP3b^177∼348 were capable of eliciting AR at different concentrations. With the addition of either peptide, the [Ca²⁺]i level was raised to a peak with or without a plateau. The AR could be inhibited by pertussis toxin (PTX), EGTA, and pimozide (a T-type calcium channel blocker), whereas verapamil was less effective in this regard. The results of the present study suggest that peptides rhuZP3a^22∼176 and rhuZP3b^177∼348 have a role similar to human ZP3, and that the mechanism of the response to the peptides involves influx of calcium, the G protein pathway, and a T-type calcium channel.</p>","PeriodicalId":15029,"journal":{"name":"Journal of andrology","volume":"28 3","pages":"381-388"},"PeriodicalIF":0.0,"publicationDate":"2013-01-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.2164/jandrol.106.001289","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"26463965","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}