Journal of Advances in Biology & Biotechnology最新文献

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Enhancing Cucurbita pepo Growth, Productivity, and Fruit Quality using Bacilli Strains and Cyanobacteria Treatments 芽孢杆菌和蓝藻处理促进葫芦生长、产量和果实品质
Journal of Advances in Biology & Biotechnology Pub Date : 2021-12-16 DOI: 10.9734/jabb/2021/v24i1130248
Ashmawi Elsayed Ashmawi, Amira M. El-Emshaty, G. M. Salem, M. F. Ghazal
{"title":"Enhancing Cucurbita pepo Growth, Productivity, and Fruit Quality using Bacilli Strains and Cyanobacteria Treatments","authors":"Ashmawi Elsayed Ashmawi, Amira M. El-Emshaty, G. M. Salem, M. F. Ghazal","doi":"10.9734/jabb/2021/v24i1130248","DOIUrl":"https://doi.org/10.9734/jabb/2021/v24i1130248","url":null,"abstract":"Two successful field experiments were carried out during 2020and 2021 growing seasons to evaluate the effect of bio fertilizers; Bacillus amyloliquifaciens (BA), Bacillus megaterium (BM) and cyanobacteria inoculation on the vegetative growth, growth parameters and plant chemical content of Cucurbita pepo (Squash) crop. The study of mixed inoculation with both Bacillus strains, and cyanobacteria was found to improve vegetative growth, plant chemical contents and positive microbial activity in the soil Rhizosphere in comparison to un-inoculated plants. Soil available nutrients (N and K) increased significantly with BA and BM combined with cyanobacteria while available phosphorus gave most increase with BM.","PeriodicalId":15023,"journal":{"name":"Journal of Advances in Biology & Biotechnology","volume":"13 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-12-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"75983831","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Mortality of Fruit Flies (Diptera: Tephritidae) Exposed to Spinetoram Toxic Bait in the Laboratory 室内果蝇(双翅目:蝗科)暴露于刺蛾毒饵后的死亡率
Journal of Advances in Biology & Biotechnology Pub Date : 2021-12-16 DOI: 10.9734/jabb/2021/v24i1130249
A. Raga, Ester Marques De Sousa, L. R. F. Louzeiro
{"title":"Mortality of Fruit Flies (Diptera: Tephritidae) Exposed to Spinetoram Toxic Bait in the Laboratory","authors":"A. Raga, Ester Marques De Sousa, L. R. F. Louzeiro","doi":"10.9734/jabb/2021/v24i1130249","DOIUrl":"https://doi.org/10.9734/jabb/2021/v24i1130249","url":null,"abstract":"Fruit flies (Diptera: Tephritidae) cause significant losses during the production and marketing of horticultural products. Brazilian growers usually adopt full-coverage insecticide spraying to control fruit flies, but toxic bait is a more strategic technique, because reach efficacy and the target surface is the foliage and branches. We provide information regarding the toxicity of spinetoram bait to two fruit fly species in the laboratory as an alternative to organophosphates and the specific spinosad formulation. We tested toxic baits in the laboratory, using commercial hydrolysed corn protein (10% v/v) plus 90 g, 120 g, 150 g and 180 g dilutions of spinetoram 250 WG (commercial product/1,000 litres of water). All toxic baits were compared with an untreated control (only protein) for the adults of females and males of Anastrepha obliqua (Macquart, 1835) and Ceratitis capitata (Wiedemann, 1824) up to 30 hours of exposure. Dry food for adults was included in all dilutions (5% w/v). In addition, we tested the residual effect of toxic baits applied to the leaves of mandarin seedlings. We used the same treatments of the earlier bioassay without dry food, collecting treated leaves and exposing them to C. capitata (medfly) females for 24 hours in the laboratory. Leaves were collected 1, 3, 7, 15 and 30 days after application. Overall, medfly adults were more susceptible to spinetoram baits than A. obliqua. All toxic baits resulted in 100% C. capitata mortality 24 hours after initial exposure, and the toxic bait at 150 g/1,000 L of water resulted in the maximum mortality (96%) in A. obliqua. Except for 90 g of spinetoram bait at 30 days after application, all spinetoram bait concentrations resulted in significantly, more dead C. capitata females than the control over all tested periods in the residual bioassay. At 30 days after application, spinetoram baits at 120 g, 150 g and 180 g resulted in 85%, 87% and 86% mortality in C. capitata, respectively. Spinetoram toxic baits have proven promising for long-term fruit fly management.","PeriodicalId":15023,"journal":{"name":"Journal of Advances in Biology & Biotechnology","volume":"12 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-12-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"90048480","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Toxicological Activity of the Methanolic Leaf Extract of Some Medicinal Plants Used in Sokoto Township and Environs 索科托镇及近郊几种药用植物甲醇叶提取物的毒理活性研究
Journal of Advances in Biology & Biotechnology Pub Date : 2021-12-15 DOI: 10.9734/jabb/2021/v24i1030247
Z. Shagari, Maimuna Umar Bello, U. K. Mohammed, A. I. Dabai, A. Mahmuda, A. Baki, A. Imam, M. Ganau
{"title":"Toxicological Activity of the Methanolic Leaf Extract of Some Medicinal Plants Used in Sokoto Township and Environs","authors":"Z. Shagari, Maimuna Umar Bello, U. K. Mohammed, A. I. Dabai, A. Mahmuda, A. Baki, A. Imam, M. Ganau","doi":"10.9734/jabb/2021/v24i1030247","DOIUrl":"https://doi.org/10.9734/jabb/2021/v24i1030247","url":null,"abstract":"Inspite of the availability of different antiseptic and antibiotics in most localities in some parts of the world, there is still a number of information on the usage of some local plants in various kinds of treatments of different ill-health conditions. Leaves of Ocimum basilicum, Leptadania hastata and Momordica balsamina are locally used by traditional birth attendants at pre and post-partum periods. The present study investigates the phytochemical compositions and toxicity of the leaf extracts of the plants against isolates of Listeria monocytogenes. Standard microbiological techniques and polymerase chain reaction was used to isolate and identify the bacteria.  Phytochemical analysis was done and cytotoxicity of the extracts at different concentrations (MBC, OBC and LHC) were determined using human erythrocytes. Results of the phytochemical analysis revealed the presence of tannins, flavonoids, carbohydrates, alkaloids, terpinoids and glycosides in the studied extracts. Toxicity to erythrocytes, expressed as percentage hemolysis of only 17.27% (MBC1) was seen in one of the plants; M. balsamina. Similarly, the other extracts exhibited minimal toxicity to human erythrocytes (LHC1= 15.45%; OBC1= 7.6%). It was concluded that all the plant leaf extracts are safe for human consumption. Studies on the preparation, effective doses and side effects of these extracts in vivo are hereby recommended.","PeriodicalId":15023,"journal":{"name":"Journal of Advances in Biology & Biotechnology","volume":"63 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-12-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"90523180","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Isolation and Identification of Anthracene Utilizing Proteus vulgaris from Oil Spill Contaminated Soil at NNPC Depot Kano State Nigeria 利用变形杆菌从尼日利亚石油公司卡诺州油库溢油污染土壤中分离鉴定蒽
Journal of Advances in Biology & Biotechnology Pub Date : 2021-12-14 DOI: 10.9734/jabb/2021/v24i1030246
U. Shehu, F. A. Ahmad, F. Yusuf, F. Muhammad, H. Yakasai
{"title":"Isolation and Identification of Anthracene Utilizing Proteus vulgaris from Oil Spill Contaminated Soil at NNPC Depot Kano State Nigeria","authors":"U. Shehu, F. A. Ahmad, F. Yusuf, F. Muhammad, H. Yakasai","doi":"10.9734/jabb/2021/v24i1030246","DOIUrl":"https://doi.org/10.9734/jabb/2021/v24i1030246","url":null,"abstract":"Microbial biodegradation of polycyclic aromatic hydrocarbons (PAHs) like Anthracene, represent an efficient, time, and cost-effective way for bioremediation of the polluted environment. This study was aimed at isolating, identifying and characterizing bacteria with potential to degrade and utilize anthracene as a sole carbon source. A bacteria was isolated from oil spilled contaminated site located in Kano, using an enrichment method on mineral salt media (MSM) following serial dilution (10-1-10-6). Characterization was done by studying the effects of temperature and pH on mineral salt media (MSM) containing anthracene. The isolate was then identified morphologically, biochemically and molecularly based on 16S rRNA partial gene sequence analysis. The morphological and microscopic examination of the isolate from this research shows that the isolate was creamy in color, motile, gram negative, short rod and non-spore forming respectively. The biochemical test of the isolate was found to be positive for these parameters (methyl red, catalase, motility, indole and urease) and negative for (citrate and oxidase). The 16S rRNA sequence and Phylogenetic analysis using neighbor joining tree and 1000 boos trap revealed that, the isolate was closely related (on the same clade) to Proteus vulgaris with accession number MW766369. Characterization was done by studying the effects of temperature and pH. The isolated bacterium was optimal at a temperature of 35ºC and pH 7.5. This isolate is a promising strain that could be used in bioremediation of polycylic aromatic hydrocarbons polluted environment.","PeriodicalId":15023,"journal":{"name":"Journal of Advances in Biology & Biotechnology","volume":"64 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-12-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"88338603","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 4
Ecological Adaptation of Endemic Anthocleista Species under Moisture Gradient in Parts of Niger Delta, Nigeria 尼日利亚尼日尔三角洲部分地区特有Anthocleista物种在水分梯度下的生态适应
Journal of Advances in Biology & Biotechnology Pub Date : 2021-12-13 DOI: 10.9734/jabb/2021/v24i1030245
Edwin-Wosu Nsirim Lucky, Omara-Achong Theresa Ebia, Idogun Ejiro Praise
{"title":"Ecological Adaptation of Endemic Anthocleista Species under Moisture Gradient in Parts of Niger Delta, Nigeria","authors":"Edwin-Wosu Nsirim Lucky, Omara-Achong Theresa Ebia, Idogun Ejiro Praise","doi":"10.9734/jabb/2021/v24i1030245","DOIUrl":"https://doi.org/10.9734/jabb/2021/v24i1030245","url":null,"abstract":"Background: Knowledge of the anatomy of Anthocleista species is crucial for understanding how these plants adapt to the environment. \u0000Aim: This study was aimed at investigating the adaptive relationship of moisture gradient influence on the anatomy of four species in the genus Anthocleista (A djalonesis A Chev; A.  liebrechtsiana De Wild & Th.Dur; A. nobilis G.Don; and A. vogelii Planch) in light of ecological niche adaptation. Place of Study: parts of Akwa-Ibom, Bayelsa, Cross River and Rivers States in the Niger Delta. Methods: Conventional classical anatomical techniques for structural sectioning were used. \u0000Results: Though there are similarities and differences in vascular structure among the species, the study has revealed variance in anatomical responses to moisture gradient (ranging from dry mesophytic to mesophytic and wetland conditions) of adaptation. The most important and distinct features observed are the presence of sclerenchymatous idioblast, air sacs and sclereidal idioblast. Sclerenchymatous idioblasts are numerous in A liebrechtsiana, few in A.  nobilis and A. vogelii but lacking in A. djalonesis. The sclerenchymatous idioblast in A. liebrechtsiana, A. nobilis, and A. vogelii confirmed these species to be mesophytic to semi-aquatic in their habitat adaptation; while A. djalonesis is dry-mesophytic in adaptation with thicker epidermal layer, multiple hypodermal layers, thicker mesophyll tissues with increased number of palisade layers and thick leaves. The stem and root modification had abundance sclereidal idioblast distribution in A. liebrechtsiana, and A. nobilis, moderate in A. vogelii and very low in A. djalonesis. \u0000Conclusion: The variation observed in the leaf, petiole, stem and root anatomical characters are due to moisture gradient influence with the resultant effect of plant species evolving structures such as idioblast and modification to adapt to the niche and environment where they find themselves.","PeriodicalId":15023,"journal":{"name":"Journal of Advances in Biology & Biotechnology","volume":"25 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-12-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"77660378","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Determination of Heavy Metals in Soil around Amasoma Community in Bayelsa State, Nigeria 尼日利亚巴耶尔萨州阿马索马社区周边土壤重金属测定
Journal of Advances in Biology & Biotechnology Pub Date : 2021-12-11 DOI: 10.9734/jabb/2021/v24i1030244
Ebimobowei Assayomo, D. Bawo, G. K. Dieware, Amaka Mariagoretti Buseri, Ebikabowei John Debekeme
{"title":"Determination of Heavy Metals in Soil around Amasoma Community in Bayelsa State, Nigeria","authors":"Ebimobowei Assayomo, D. Bawo, G. K. Dieware, Amaka Mariagoretti Buseri, Ebikabowei John Debekeme","doi":"10.9734/jabb/2021/v24i1030244","DOIUrl":"https://doi.org/10.9734/jabb/2021/v24i1030244","url":null,"abstract":"Aims: Anthropogenic activities such as crude oil exploration and exploitation has led to the suspicion of heavy metals contamination in the study area. This study investigated the concentration of zinc, nickel, lead and cadmium in the soil extracted from the area where Scent Leaf (Ocimum gratissimum) and Pawpaw tree (Carica papaya) are grown in Amassoma community, Bayelsa State, Nigeria. \u0000Study Design: Scent leaf (Ocimum gratissimum) and Pawpaw tree (Carica papaya) soils from where these plants are grown were randomly collected for heavy metal analysis from within Amassoma community in Southern Ijaw Local Government Area of Bayelsa State, Nigeria \u0000Place and Duration of Study: This study was carried out in Biological Sciences, Faculty of Science, Niger Delta University, Wilberforce Island, Amassoma, Bayelsa State between March 2019 to September 2019 \u0000Methodology:  Scent leaf (Ocimum gratissimum) and Pawpaw tree (Carica papaya) soils were digested and analyzed for heavy metals using Atomic Absorption Spectrophotometer (AAS, model S471096). The method used was wet-ashing method for the analysis. \u0000Results: the concentrations of Lead (Pb), Nickel (Ni), Cadmium (Cd), and Zinc (Zn), in pawpaw soil and scent leaf soil. From the sample data analysis, the average lead (Pb) contents in pawpaw soil and scent leaf soil were 1.283 mg/kg and 1.44 mg/kg respectively. The average Cadmium (Cd) in pawpaw soil and scent leaf soil were 0.256 mg/kg and 0.256 mg/kg respectively. The average Nickel (Ni) compositions in pawpaw soil and scent leaf soil were 4.71 mg/kg and 5.09 mg/kg respectively. While the Average Zinc (Zn) in pawpaw soil and scent leaf soil is 2.280 mg/kg and 2.160 mg/kg respectively. The soil analyzed for heavy metals were below the threshold target values recommended by the WHO. \u0000Conclusion: The Scent leaf (Ocimum gratissimum) and Pawpaw tree (Carica papaya) soils analyzed for heavy metals were below the permissible limit recommended by WHO for soil safety. The result from the present study revealed that there may not be soil contamination as a result of the selected heavy metals, however, It is recommended that caution should be taken in regulating the anthropogenic activities that may tend to elevate the level of heavy metals in the surrounding to prevent contamination of soil over time which could be detrimental to the health of the populace.","PeriodicalId":15023,"journal":{"name":"Journal of Advances in Biology & Biotechnology","volume":"69 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-12-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"73020974","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 2
Comparative Review of Plant Transformation Techniques 植物转化技术比较综述
Journal of Advances in Biology & Biotechnology Pub Date : 2021-12-10 DOI: 10.9734/jabb/2021/v24i1030243
A. okpe, F. Nkaa
{"title":"Comparative Review of Plant Transformation Techniques","authors":"A. okpe, F. Nkaa","doi":"10.9734/jabb/2021/v24i1030243","DOIUrl":"https://doi.org/10.9734/jabb/2021/v24i1030243","url":null,"abstract":"Plant transformation is now an important biotechnological tool in plant biology and a practical tool for transgenic plant development. There are many verified methods for stable introduction of novel genes into the nuclear genomes of diverse plant species. As a result, gene transfer and regeneration of transgenic plants are no longer the factors limiting the development and application of practical transformation systems for many plant species. However, the desire for higher transformation efficiency has stimulated work on not only improving various existing methods but also in inventing novel methods. Different methods of transferring the gene into plant cells have been developed and continuous efforts have been made to increase its efficiency. Both direct and indirect methods of gene transfer have their own merits and demerits. Efforts have been made continuously to eliminate drawbacks and to develop an easy and eco-friendly method to transfer foreign genes. Many methods of genetic transformation have been proposed and tried in the laboratories, but most of them result to transient expressions. However, transformation work based on particle bombardment with DNA coated micro projectiles and Agrobacterium mediated transformation have proved to be promising in producing stable transgenic plants from a range of plant species.","PeriodicalId":15023,"journal":{"name":"Journal of Advances in Biology & Biotechnology","volume":"26 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-12-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"86667643","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 1
Determination of the Influence of Cultural Techniques "Densities and Opening Standardʺ on Agrophysiological Parameters of Clones Pb 260, Irca 111 and Rrim 703 栽培技术“密度和开放标准”对pb260、irca111和Rrim 703无性系农业生理参数影响的测定
Journal of Advances in Biology & Biotechnology Pub Date : 2021-12-04 DOI: 10.9734/jabb/2021/v24i930240
Diarrassouba Moussa, Soumahin Eric Francis, Konan Djézou, N’guessan Kan Pulchérie, Moro Affia Perpétue, Koffi Antoine, Obouayeba Samuel
{"title":"Determination of the Influence of Cultural Techniques \"Densities and Opening Standardʺ on Agrophysiological Parameters of Clones Pb 260, Irca 111 and Rrim 703","authors":"Diarrassouba Moussa, Soumahin Eric Francis, Konan Djézou, N’guessan Kan Pulchérie, Moro Affia Perpétue, Koffi Antoine, Obouayeba Samuel","doi":"10.9734/jabb/2021/v24i930240","DOIUrl":"https://doi.org/10.9734/jabb/2021/v24i930240","url":null,"abstract":"To find out the effect of cultural techniques on agrophysiological parameters, four combinations ̏planting densities (low density or DF at 350 t/ha, normal density or DN at 510 t/ha) and opening standards (opening at 65cm and opening at 50 cm) ̋ were tested on Hevea brasiliensis clones PB 260, IRCA 111 and RRIM 703. This study was conducted for nine years with a split-plot experimental design of two treatments and two subtreatments repeated three times. The different combinations of treatments and subtreatments tested were low density (DN-50 cm), low density (DN-65 cm), normal density (DF-50 cm), normal density (DF- 65 cm). The parameters evaluated were the production at bleeding and per hectare, the average annual increase in circumference, the rate of tree losses and the rate of trees with dry notches. Production per tree was significantly higher at 350 t/ha and 65 cm opening (DF-65 cm), while per hectare production was higher at 510 t/ha. The girths of the different clones are stronger at DF and at the 65 cm opening. The rate of tree loss and the rate of trees with dry notch were low at the 510 t/ha density and the 65cm opening. The appropriate density and opening standard was \"normal density 510 t/ha and opening to circumference 50 cm\". The cultivation techniques especially the density and opening standard judiciously applied can participate in the sustainable improvement of rubber productivity of rubber plantations.","PeriodicalId":15023,"journal":{"name":"Journal of Advances in Biology & Biotechnology","volume":"29 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-12-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"88049152","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Molecular Characterization of Fungi Associated with Dump Site Soil 垃圾场土壤真菌的分子特性研究
Journal of Advances in Biology & Biotechnology Pub Date : 2021-12-04 DOI: 10.9734/jabb/2021/v24i930239
N. Ogbuji, A. Ataga, P. Tari-Ukuta, C. J. Olisedeme
{"title":"Molecular Characterization of Fungi Associated with Dump Site Soil","authors":"N. Ogbuji, A. Ataga, P. Tari-Ukuta, C. J. Olisedeme","doi":"10.9734/jabb/2021/v24i930239","DOIUrl":"https://doi.org/10.9734/jabb/2021/v24i930239","url":null,"abstract":"Aims: A study was conducted to identify fungal species isolated from dumpsite soil in University of Port Harcourt using molecular techniques. \u0000Methodology: Molecular methods for determining the species of a fungus based on the amplification and sequencing of the internal subscribed spacer (ITS) region of the fungal rRNA operon using molecular markers was applied. Soil sample was collected from a dumpsite in the University of Port Harcourt, Rivers State, Nigeria. Isolation of fungi associated with the dumpsite soil was carried out using spread plate method. Fungal genomic DNA was extracted using Quick-DNA Fungal/Bacterial Miniprep kit. The ITS1-2 gene of the isolates was amplified by Polymerase Chain Reaction (PCR) using the primer pair; ITS4 and ITS5. \u0000Results: The sequences of the amplified ITS region were blasted against known sequences on the National Centre for Biotechnology Information (NCBI) database. Nucleotide sequence analysis revealed the species identity of the fungal isolates to be: Aspergillus fumigatus, Trichoderma harzianum, Aspergillus felis, Aspergillus templicola, Aspergillus flavipes, Aspergillus fumigatus and Cunninghamella binariae. Phylogenetic analysis was carried out to ascertain the relationship between the isolates and other closely-related isolates on GenBank. Isolates 2 (Trichoderma harzianum) and 7 (Cunninghamella binariae), 3 (Aspergillus felis) and 6 (Aspergillus fumigatus), and 4 (Aspergillus templicola) and 5 (Aspergillus flavipes) were found to be more closely related to each other. \u0000Conclusion: The molecular techniques employed successfully identified the organisms to the species level as these techniques are based on the genetic constitution of organisms. The result obtained from this study will complement the information on the fungal organisms associated with dumpsite soil.","PeriodicalId":15023,"journal":{"name":"Journal of Advances in Biology & Biotechnology","volume":"29 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-12-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"85321579","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Callus Induction, Regeneration and Establishment of Rice Plant from Mature Embryo 水稻成熟胚愈伤组织诱导、再生和植株建立
Journal of Advances in Biology & Biotechnology Pub Date : 2021-11-27 DOI: 10.9734/jabb/2021/v24i930238
Nazmul Alam Khan, M. Uddin, M. Rana, N. Jahan, Mirana Akhter Sumi, M. Rashid
{"title":"Callus Induction, Regeneration and Establishment of Rice Plant from Mature Embryo","authors":"Nazmul Alam Khan, M. Uddin, M. Rana, N. Jahan, Mirana Akhter Sumi, M. Rashid","doi":"10.9734/jabb/2021/v24i930238","DOIUrl":"https://doi.org/10.9734/jabb/2021/v24i930238","url":null,"abstract":"Plant growth regulators were used to test callus induction and in vitro regeneration in six rice genotypes (RM-AC-2, BRRI dhan89, BRRI dhan88, Nipponbare, Koshihikari and Zenshan97). Four different concentrations (1, 2, 3 and 4 mg/L) of 2,4-D for callus induction and three different concentrations (1,2 and 3 mg/L) of NAA with three doses (5,10 and 15 µ/L) of kinetin for callus regeneration were used to test the effect of plant growth regulators. This study found a high callus induction on MS medium enriched with 2 mg/L 2, 4-D. In cases of RM-AC-2, BRRI dhan89, BRRI dhan88, Nipponbare, Koshihikari and Zenshan97, callus induction frequencies were 92.7%, 87.8%, 84.6%, 82.9%, 86.2% and 62.9%, respectively. In the regeneration, it was found that an MS medium enriched with 2 g/L Kinetin and 10 µm/L NAA has the ability to induce increased regeneration of different rice varieties (RM-AC-2 (72.4%), BRRI dhan89 (66.9%), BRRI dhan88 (62.5%), Nipponbare (63.3%), Koshihikari (48%) and Zenshan97 (39.6%). From the regenerated plants, one plant of the RM-AC-2 genotype availed to complete its life cycle and generated 32 effective tillers and yielded 89g. This rice plant is very promising for high yielding rice variety development program in Bangladesh. The improved callus development and regeneration ability of this genotype might be helpful for future rice variety development and genetic transformation program.","PeriodicalId":15023,"journal":{"name":"Journal of Advances in Biology & Biotechnology","volume":"58 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-11-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"77530577","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 1
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