Journal of Apicultural Science最新文献_第8页

Dispersion of Bumblebee Bombus Semenoviellus Skorikov (Hymenoptera, Apidae) in Poland 波兰大黄蜂（膜翅目，蜂科）的传播

IF 0.6 4区农林科学

Journal of Apicultural Science Pub Date : 2020-06-01 DOI: 10.2478/jas-2020-0006

Paweł Michołap, A. Sikora, T. Pawlikowski, Marcin Sikora

引用次数: 1

Biochemical Isolation and Characterization of Hyaluronidase Enzyme from Venom of Egyptian Honey Bee Apis Mellifera Lamarckii 埃及蜜蜂(Apis Mellifera Lamarckii)毒液中透明质酸酶的生化分离与特性研究

IF 0.6 4区农林科学

Journal of Apicultural Science Pub Date : 2020-06-01 DOI: 10.2478/jas-2020-0015

Mohammed M. Abdel-Monsef, Hind A. Zidan, Doaa A. Darwish, H. Masoud, M. Helmy, M. A. Ibrahim

{"title":"Biochemical Isolation and Characterization of Hyaluronidase Enzyme from Venom of Egyptian Honey Bee Apis Mellifera Lamarckii","authors":"Mohammed M. Abdel-Monsef, Hind A. Zidan, Doaa A. Darwish, H. Masoud, M. Helmy, M. A. Ibrahim","doi":"10.2478/jas-2020-0015","DOIUrl":"https://doi.org/10.2478/jas-2020-0015","url":null,"abstract":"Abstract The hyaluronidase enzyme has been used in many such fields of medicine as ophthalmology, orthopaedia, internal medicine, gynecology, surgery, oncology and dermatology. In this study, the hyaluronidase enzyme was purified and characterized for the first time from Egyptian bee venom homogeneously using DEAE-cellulose and Sephacryl S-300 columns. Bee venom hyaluronidase specific activity was 411.7 units/mg protein with 49.9% yield and 3.23-fold purification. The molecular weight of the purified bee venom hyaluronidase native form was 37 kDa. The purified enzyme was found homogeneous on native PAGE and SDS-PAGE, with two congruent subunits of 18.4 kDa and isoelectric point (pI) of 8.6–8.8. The enzyme was found to be stable over a wide range of temperature (20–60°C) and pH (4.5–6.5), and its optimum activity at 37°C, pH 5.4 and 0.15 M NaCl. Km for bee venom hyaluronidase was 0.029 mg/ml hyaluronic acid and its activity was elevated in presence of MgCl2 and ZnCl2 and lowered in presence of FeCl2. Heparin inhibited the hyaluronidase enzyme noncompetitively with a Ki value of 2.9 units heparin and one binding site on the enzyme molecule.","PeriodicalId":14941,"journal":{"name":"Journal of Apicultural Science","volume":"64 1","pages":"153 - 164"},"PeriodicalIF":0.6,"publicationDate":"2020-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42083125","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 5

Foraging Behavior of Honeybees (Apis Mellifera L.) and Ground Bumblebees (Bombus Terrestris L.) and its Influence on Seed Yield and Oil Quality of Oil Tree Peony Cultivar ‘Fengdan’ (Paeonia Ostii T. Hong et J. X. Zhang) 蜜蜂(Apis Mellifera L.)和地熊蜂(Bombus Terrestris L.)的觅食行为及其对油树牡丹‘凤丹’(Paeonia Ostii T. Hong等)种子产量和油质的影响

IF 0.6 4区农林科学

Journal of Apicultural Science Pub Date : 2020-06-01 DOI: 10.2478/jas-2020-0014

Chunlin He, Kaiyue Zhang, Dongbo Han, Shuaibing Wang, Xiaogai Hou, Chaodong Zhu

{"title":"Foraging Behavior of Honeybees (Apis Mellifera L.) and Ground Bumblebees (Bombus Terrestris L.) and its Influence on Seed Yield and Oil Quality of Oil Tree Peony Cultivar ‘Fengdan’ (Paeonia Ostii T. Hong et J. X. Zhang)","authors":"Chunlin He, Kaiyue Zhang, Dongbo Han, Shuaibing Wang, Xiaogai Hou, Chaodong Zhu","doi":"10.2478/jas-2020-0014","DOIUrl":"https://doi.org/10.2478/jas-2020-0014","url":null,"abstract":"Abstract Oil peony (Paeonia spp.) is a new type of woody oil crop in China with a large cultivation area. Inadequate pollination is one of the main reasons for low seed yield. A pollination net room was built at an oil tree peony base, the numbers of honeybees (Apis mellifera L.) and ground bumblebees (Bombus terrestris L.) were artificially increased and the foraging behaviors and daily activities of the two bees on the plants were observed. Four different pollination methods (honeybee pollination, ground bumblebee pollination, natural field pollination and pollination without insects) were applied. The visit interval, single-flower visit time, number of single-flower visits, number of flowers visited per minute and number of stigma contacts were compared. Meanwhile, the effects of honeybee and bumblebee pollination on the oil yield and quality of peony seeds were compared. There were noticeable differences in daily activity between honeybees and ground bumblebees. Significant differences in the single-flower visit time, visit interval and visit frequency were also observed; honeybee and ground bumblebee pollination increased the seed yield of oil tree peony by 78.74% and 31.88%, respectively. Therefore, both honeybees and ground bumblebees are effective pollinators of oil tree peony. These results provide a theoretical basis for further utilization of bee resources for oil tree peony pollination.","PeriodicalId":14941,"journal":{"name":"Journal of Apicultural Science","volume":"64 1","pages":"131 - 142"},"PeriodicalIF":0.6,"publicationDate":"2020-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42214589","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 4

Effects of Bacterial Cell-Free Supernatant on Nutritional Parameters of Apis Mellifera and Their Toxicity Against Varroa Destructor 细菌无细胞上清液对蜜蜂营养参数的影响及其对瓦罗华破坏因子的毒性

IF 0.6 4区农林科学

Journal of Apicultural Science Pub Date : 2020-06-01 DOI: 10.2478/jas-2020-0009

F. D. De Piano, M. Maggi, Facundo R. Meroi Arceitto, M. Audisio, M. Eguaras, S. Ruffinengo

{"title":"Effects of Bacterial Cell-Free Supernatant on Nutritional Parameters of Apis Mellifera and Their Toxicity Against Varroa Destructor","authors":"F. D. De Piano, M. Maggi, Facundo R. Meroi Arceitto, M. Audisio, M. Eguaras, S. Ruffinengo","doi":"10.2478/jas-2020-0009","DOIUrl":"https://doi.org/10.2478/jas-2020-0009","url":null,"abstract":"Abstract Apis mellifera L. is an essential pollinator that is currently being affected by several stressors that disturb their ecological function and produce colony losses. Colonies are being seriously affected by the ectoparasitic mite Varroa destructor. The relationship between stressors and bee symbionts is being studied in order to enhance bee health. The goal of this study was to evaluate the effect of cell-free supernatants (CFSs) produced by Lactobacillus johnsonii AJ5, Enterococcus faecium SM21 and Bacillus subtilis subsp. subtilis Mori2 on A. mellifera nutritional parameters and their toxicity against V. destructor. Toxicity and survival bioassays were conducted on adult bees with different concentrations of CFSs. Nutritional parameters such as soluble proteins and fat bodies in abdomens were measured. Varroa destructor toxicity was analyzed by a contact exposure method and via bee hemolymph. At low concentrations, two of CFSs tends to enhance bee survival. Remarkably fat bodies maintained their levels with all CFS concentrations in the abdomens, and soluble protein increased at a high concentration of two CFSs. Toxicity against V. destructor was observed only via hemolymph, and results were in agreement with the treatment that produced an increase in bee proteins. Finally, CFS produced by L. johnsonii AJ5 could be a promising natural alternative for strengthening bee health.","PeriodicalId":14941,"journal":{"name":"Journal of Apicultural Science","volume":"64 1","pages":"55 - 66"},"PeriodicalIF":0.6,"publicationDate":"2020-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42752592","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 4

Radiation Disinfestation of Honeybee Combs Infested with Greater Wax Moth Eggs 大蜡蛾卵感染蜂窝的辐射消毒

IF 0.6 4区农林科学

Journal of Apicultural Science Pub Date : 2020-06-01 DOI: 10.2478/jas-2020-0008

M. Mansour

{"title":"Radiation Disinfestation of Honeybee Combs Infested with Greater Wax Moth Eggs","authors":"M. Mansour","doi":"10.2478/jas-2020-0008","DOIUrl":"https://doi.org/10.2478/jas-2020-0008","url":null,"abstract":"Abstract The sensitivity of different developmental stages of greater wax moth (Galleria mellonella L.) eggs to gamma irradiation was examined. Eggs, 25 to 144 h-old, were exposed at 48 h intervals to gamma radiation dosages (50 to 400 Gy), and the effects on egg hatch, pupation and adult emergence were investigated. The developmental rate of irradiated-egg larvae and pupae to adult stage was also studied. Results showed that the irradiation sensitivity of G. mellonella eggs decreased with increased age of the eggs when irradiated. In 25–48 h-old eggs, 50 Gy dose significantly reduced egg hatch and at 200 Gy dose no egg hatch was observed. Eggs 73–96 h-old were slightly more tolerant to irradiation treatment; hatching completely ceased at 250 Gy dose. Sensitivity to gamma irradiation reached its lowest level however when 121–144 h-old eggs (few h. before egg hatch) were irradiated. 350 Gy dose reduced egg hatch to about 3%, and at 400 Gy no egg hatch was noted. Survival to the adult stage was also negatively affected. At the most radio-tolerant stage (121–144 h-old), 100 Gy dose significantly reduced larval survival to the adult stage and 300 Gy completely prevented it. The development rate of larvae and pupae resulting from irradiated eggs was also negatively affected.","PeriodicalId":14941,"journal":{"name":"Journal of Apicultural Science","volume":"64 1","pages":"37 - 46"},"PeriodicalIF":0.6,"publicationDate":"2020-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45961499","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

Integration of NDVI Imagery and Crop Coverage Registration System for Apiary Schedule 蜂房时间表NDVI影像与作物覆盖登记系统的整合

IF 0.6 4区农林科学

Journal of Apicultural Science Pub Date : 2020-06-01 DOI: 10.2478/jas-2020-0011

F. Sarı, I. Kandemir, D. Ceylan

{"title":"Integration of NDVI Imagery and Crop Coverage Registration System for Apiary Schedule","authors":"F. Sarı, I. Kandemir, D. Ceylan","doi":"10.2478/jas-2020-0011","DOIUrl":"https://doi.org/10.2478/jas-2020-0011","url":null,"abstract":"Abstract Beekeepers need to establish migratory apiaries to benefit from pollen and nectar source plants as in order to increase honey yield. Thus, following the flowering seasons of honey source plants has vital importance when deciding the route of migration. In this study, MODIS imagery was used to generate weekly NDVI data between 1st April to 31st August 2018, when beekeeping activities start and end in the study area. Although MODIS images have high temporal resolution, low spatial resolution (250 meters) makes them insufficient when deciding the crop types and plants. While detecting plants in natural plant areas requires high spatial resolution NDVI, Crop Coverage Registration System (CCRS) parcel-based crop coverage records can enrich the NDVI data without increasing spatial resolution in agricultural lands. Thus, the CCRS data were integrated with NDVI images for migratory beekeeping in agricultural areas as an innovation. To generate both high temporal and spatial resolution, NDVI and CCRS data were integrated together with a beekeeping suitability map to generate the apiary schedule. The results were verified with 176 existing apiary locations and production dates retrieved from field studies which revealed the existence of three seasons in the study area as early and late apiaries (in natural plant areas) and apiaries in agricultural lands. Accuracy analysis showed that 82% of the apiaries intersected with suitable locations and that apiaries in agricultural areas were detected five days earlier than in field studies and obtained more accurately than natural plant apiaries.","PeriodicalId":14941,"journal":{"name":"Journal of Apicultural Science","volume":"64 1","pages":"105 - 121"},"PeriodicalIF":0.6,"publicationDate":"2020-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44300409","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 4

Sudden and Prevalent Deaths of Foraging Honey Bees in Early Spring During Sowing of Clothianidin Coated Maize Seeds Between 2013 and 2018 in Turkey 2013年至2018年土耳其播种衣虫胺包衣玉米种子期间早春觅食蜜蜂的突然和普遍死亡

IF 0.6 4区农林科学

Journal of Apicultural Science Pub Date : 2020-06-01 DOI: 10.2478/jas-2020-0007

H. Oruç, Meltem Çayci, Rashad Sariyev

引用次数: 0

Lotmaria Passim As Third Parasite Gastrointestinal Tract of Honey Bees Living in Tree Trunk 番莲为生活在树干中的蜜蜂的第三寄生胃肠道

IF 0.6 4区农林科学

Journal of Apicultural Science Pub Date : 2020-06-01 DOI: 10.2478/jas-2020-0012

M. Michalczyk, A. Bancerz-Kisiel, R. Sokół

{"title":"Lotmaria Passim As Third Parasite Gastrointestinal Tract of Honey Bees Living in Tree Trunk","authors":"M. Michalczyk, A. Bancerz-Kisiel, R. Sokół","doi":"10.2478/jas-2020-0012","DOIUrl":"https://doi.org/10.2478/jas-2020-0012","url":null,"abstract":"Abstract Honey bees (Apis mellifera L.) inhabiting trees in forests are not managed by humans or treated for pathogens; therefore, many researchers and beekeepers believe that viral, bacterial, and parasitic diseases may lead to their decline. The aim of the study was to evaluate the prevalence of L. passim and Nosema spp. in feral colonies by real-time PCR. This study was performed on twenty-six samples of honey bees inhabiting tree trunks in north-eastern Poland. One sample consisted of sixty worker bee abdomens collected from hollow trees. Honey bees were sampled only from naturally colonized sites. Amplicons of the three evaluated pathogens were detected in twenty of the twenty-six tested samples. A significant correlation was observed between infection with three pathogens (N. apis, N. ceranae, L. passim) (r = 0.84) compared to infection with only two pathogens (N. apis and N. ceranae) (r = 0.49). N. ceranae was the predominant pathogen, but infections with various severity caused by L. passim were also noted in fourteen of the twenty-six tested samples. In view of the general scarcity of epidemiological data concerning coinfections with Nosema spp. and L. passim in honey bees in tree trunks in other countries, further research is needed to confirm the effect of concurrent pathogenic infections on the decline of bee colonies.","PeriodicalId":14941,"journal":{"name":"Journal of Apicultural Science","volume":"64 1","pages":"143 - 151"},"PeriodicalIF":0.6,"publicationDate":"2020-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42771213","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 5

Pesticide Residues in Honey from Stingless Bee Melipona Subnitida (Meliponini, Apidae) 无刺蜂蜂蜜中的农药残留

IF 0.6 4区农林科学

Journal of Apicultural Science Pub Date : 2020-06-01 DOI: 10.2478/jas-2020-0010

C. Pinheiro, F. A. Oliveira, S. C. Oloris, J. A. D. da Silva, B. Soto‐Blanco

{"title":"Pesticide Residues in Honey from Stingless Bee Melipona Subnitida (Meliponini, Apidae)","authors":"C. Pinheiro, F. A. Oliveira, S. C. Oloris, J. A. D. da Silva, B. Soto‐Blanco","doi":"10.2478/jas-2020-0010","DOIUrl":"https://doi.org/10.2478/jas-2020-0010","url":null,"abstract":"Abstract The present study aimed to detect pesticide residues in the honey produced by the stingless bee Melipona subnitida. A total of thirty-five samples of honey from M. subnitida were collected from twelve municipalities of the semiarid region of Rio Grande do Norte state, northeastern Brazil. Of these thirty-five samples, fourteen were from colonies raised in an urban area, while the other twenty-one were from the countryside. The pesticides in the samples were extracted using a modified QuEChERS method. The simultaneous analysis of 116 analytes in the honey samples was performed using liquid chromatography-tandem mass spectrometry (LC-MS/MS). Twenty-five samples (71.4% of samples) were contaminated by some amount of pesticide, and of them twenty-four had just one pesticide and one had three. The detected compounds included organophosphate pesticides (OPP) monocrotophos (24 samples), trichlorfon (6 samples) and chlorpyrifos-methyl (2 samples). In conclusion, the honey produced by the stingless bee M. subnitida may be contaminated by pesticides, which emphasizes the need for food monitoring before commercialization.","PeriodicalId":14941,"journal":{"name":"Journal of Apicultural Science","volume":"64 1","pages":"29 - 36"},"PeriodicalIF":0.6,"publicationDate":"2020-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43376435","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 6

Flowering, Forage Value, and Insect Pollination in Borage (Borago Officinalis L.) Cultivated in Se Poland 琉璃苣的开花、饲料价值和昆虫传粉波兰东南部栽培

IF 0.6 4区农林科学

Journal of Apicultural Science Pub Date : 2020-06-01 DOI: 10.2478/jas-2020-0005

E. Stawiarz, A. Wróblewska, M. Masierowska, D. Sadowska

引用次数: 1