Journal of Apicultural Science最新文献

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Quality of Propolis Commercially Available on Podkarpacki Beekeeping Market 波德卡尔帕基养蜂市场上商业蜂胶的质量
IF 0.6 4区 农林科学
Journal of Apicultural Science Pub Date : 2024-05-02 DOI: 10.2478/jas-2024-0004
M. Miłek, E. Ciszkowicz, G. Zaguła, D. Grabek-Lejko, A. Pasternakiewicz, Katarzyna Lecka-Szlachta, M. Dżugan
{"title":"Quality of Propolis Commercially Available on Podkarpacki Beekeeping Market","authors":"M. Miłek, E. Ciszkowicz, G. Zaguła, D. Grabek-Lejko, A. Pasternakiewicz, Katarzyna Lecka-Szlachta, M. Dżugan","doi":"10.2478/jas-2024-0004","DOIUrl":"https://doi.org/10.2478/jas-2024-0004","url":null,"abstract":"\u0000 Eight samples of propolis commercially available on south-eastern Poland’s beekeeping market were compared in terms of quality, chemical composition and biological activity, including antioxidant and antimicrobial properties. The organoleptic characteristics and physicochemical requirements indicated that only 50% of tested propolis samples was classified to class II regarding obligatory limits, while the others were out-class. A big variation in mineral composition was determined by the ICP-OES method but all samples were free of heavy metals (Pb, Cd and Ni). Although mesophilic bacteria, yeasts and moulds occurred in tested propolis (in general lower than 102 CFU/g), E. coli and spore-forming sulphite-reducing bacteria as well as Salmonella and Shigella bacteria were not detected. High antioxidant activity and total phenolic content for all samples was confirmed, which was correlated with antibacterial activity tested against E. coli ATCC 11775 strain. Additionally, the propolis sample with the strongest antibacterial activity (MIC 0.33 mg/mL) inhibited biofilm formation of S. aureus and S. epidermidis ATCC 35984 (MBIC 0.66 and 5.25 mg/mL, respectively). However, great variation in terms of antioxidant activity and HPLC polyphenolic profile were observed, and sakuranetin and pinobanksin were identified as the most effective antioxidant components. Moreover, the usefulness of ATR-FTIR spectra analysis was confirmed as a quick method for initial testing of propolis quality.","PeriodicalId":14941,"journal":{"name":"Journal of Apicultural Science","volume":null,"pages":null},"PeriodicalIF":0.6,"publicationDate":"2024-05-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141018030","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Case of Willow Rust Spores (Melampsora Spp.) Collected by Honey Bees 蜜蜂采集柳锈菌孢子(Melampsora Spp.)
IF 0.6 4区 农林科学
Journal of Apicultural Science Pub Date : 2024-04-19 DOI: 10.2478/jas-2024-0003
P. Migdał, Janusz Mazurek, Agata Kaczmarek-Pieńczewska, Marta Jurga-Zotow, A. Murawska
{"title":"Case of Willow Rust Spores (Melampsora Spp.) Collected by Honey Bees","authors":"P. Migdał, Janusz Mazurek, Agata Kaczmarek-Pieńczewska, Marta Jurga-Zotow, A. Murawska","doi":"10.2478/jas-2024-0003","DOIUrl":"https://doi.org/10.2478/jas-2024-0003","url":null,"abstract":"\u0000 Honey bee workers (Apis mellifera L.) collect nectar, honeydew and pollen from plants in orderto provide the colony with, among others, carbohydrates and protein. Whenever these sources are unavailable in the environment, bees turn to alternatives. An example of this are fungi spores collected accidentally or on purpose. This last phenomenon is the aim of this study, in which we have shown that worker bees can collect willow rust (Melampsora spp.) spores. We observed as the bees obtained spores and placed them in pollen baskets. The presence of spores was demonstrated with the use of a scanning electron microscope (SEM). This observation indicates that the honey bee may use alternative sources as a potential supplement. There are few studies in this field and it requires a deeper analysis.","PeriodicalId":14941,"journal":{"name":"Journal of Apicultural Science","volume":null,"pages":null},"PeriodicalIF":0.6,"publicationDate":"2024-04-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140684149","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Occurrence of Lotmaria passim in Africanized and European Honey Bee, Apis mellifera, Lineages from the United States 在美国的非洲化蜜蜂和欧洲蜜蜂蜂系中出现 Lotmaria passim 的情况
IF 0.6 4区 农林科学
Journal of Apicultural Science Pub Date : 2024-03-29 DOI: 10.2478/jas-2024-0002
Mary-Kate Williams, D. Cleary, Allen Szalanski
{"title":"Occurrence of Lotmaria passim in Africanized and European Honey Bee, Apis mellifera, Lineages from the United States","authors":"Mary-Kate Williams, D. Cleary, Allen Szalanski","doi":"10.2478/jas-2024-0002","DOIUrl":"https://doi.org/10.2478/jas-2024-0002","url":null,"abstract":"\u0000 Honey bee populations in the United States have been under stress for the past several decades. Several internal parasites may contribute to this, including the trypanosome Lotmaria passim. It is unknown how widespread the honey bee parasite, L. passim, is in Africanized honey bee (AHB) populations in the United States. A total of 321 feral honey bee colony samples which had been previously recorded to be of Africanized origin using molecular diagnostics from California (n=3), New Mexico (n=46), Oklahoma (n=57), Texas (n=106), and Utah (n=109) were used in this study. Of these samples, a total of 15 (4.7%) from three States were positive for L. passim. Utah AHB samples had the highest infection rate (11.0%), followed by Texas (1.9%) and Oklahoma (1.8%). Compared with previous studies on the occurrence of L. passim from European honey bees from the same sampled States, infection rates of Africanized honey bees for L. passim were significantly higher in the State of Utah, but not for the other sampled States. This study provides evidence that feral honey bee populations do not necessarily have lower levels of honey bee parasites than managed honey bee colonies.","PeriodicalId":14941,"journal":{"name":"Journal of Apicultural Science","volume":null,"pages":null},"PeriodicalIF":0.6,"publicationDate":"2024-03-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140365868","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Size and Shape Differences in Fore Wings of Honey Bee (Apis mellifera) Queens, Workers and Drones 蜜蜂(Apis mellifera)蜂王、工蜂和无人机前翅的大小和形状差异
IF 0.6 4区 农林科学
Journal of Apicultural Science Pub Date : 2024-03-29 DOI: 10.2478/jas-2023-0013
A. Tofilski, Hardeep Kaur, S. Łopuch
{"title":"Size and Shape Differences in Fore Wings of Honey Bee (Apis mellifera) Queens, Workers and Drones","authors":"A. Tofilski, Hardeep Kaur, S. Łopuch","doi":"10.2478/jas-2023-0013","DOIUrl":"https://doi.org/10.2478/jas-2023-0013","url":null,"abstract":"\u0000 The three honey bee phenotypes - queens, workers, and drones differ in the size and shape of body parts. We used a large dataset to describe how the three phenotypes differed with regard to fore wings and applied the methodology of geometric morphometrics to measure the wings using the coordinates of nineteen landmarks. On average, queens had wings larger than workers, but the two castes had a broad overlap. Drones, on the other hand, had distinctly larger wings. Wing shape differed markedly among queens, workers and drones and can be used to reliably differentiate them from one other. Surprisingly, the fore wing shape of workers was more similar to that of drones than to that of queens. Small queens were not more similar to workers than large queens, and large workers were not more similar to queens than small workers. Because wing size, unlike body mass, does not change over the life of the queen, it can be used to evaluate the quality of a queen throughout its life and not only during a short period after emergence. We provide a large number of wing images of queens and drones, which can be used as a reference in future studies.","PeriodicalId":14941,"journal":{"name":"Journal of Apicultural Science","volume":null,"pages":null},"PeriodicalIF":0.6,"publicationDate":"2024-03-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140366475","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Haplotype Affiliation of Varroa destructor in Selected Locations from Poland and Ukraine 波兰和乌克兰部分地区破坏者 Varroa 的单倍型隶属关系
IF 0.6 4区 农林科学
Journal of Apicultural Science Pub Date : 2024-03-29 DOI: 10.2478/jas-2024-0001
Piotr Dziechciarz, Marcin Domaciuk, M. Iller, Stefan Kerek, Grzegorz Borsuk
{"title":"Haplotype Affiliation of Varroa destructor in Selected Locations from Poland and Ukraine","authors":"Piotr Dziechciarz, Marcin Domaciuk, M. Iller, Stefan Kerek, Grzegorz Borsuk","doi":"10.2478/jas-2024-0001","DOIUrl":"https://doi.org/10.2478/jas-2024-0001","url":null,"abstract":"\u0000 The virulence patterns observed in viruses transmitted by Varroa destructor implied a potential association of these honey bee pests in Poland with the Korean haplotype. Nevertheless, it is noteworthy that this hypothesis lacked direct verification. Therefore, the aim of the study was to determine the haplotype affiliation of V. destructor mites infesting bees in Poland and Ukraine. Adult females were collected from drone brood from honey bee colonies maintained in the apiary at the University of Life Sciences in Lublin, Poland, and the apiary in Synevyr National Park in Ukraine. The haplotype affiliation was determined through the sequencing of the cytochrome oxidase subunit I gene (CO I; 929 bp) fragment. Based on the phylogenetic comparison with 84% bootstrap support, the V. destructor females from both locations were confirmed to represent three haplotypes: Korean 1 (AmK1-1), Korean 2 (AmK1-2), and Chinese 4 (AmK1-4). They are the most virulent types of this parasite worldwide and in Poland. Concurrently, the present study confirms that the Synevyr National Park in Ukraine is isolated from alien bee subspecies, but is not free from alien parasites.","PeriodicalId":14941,"journal":{"name":"Journal of Apicultural Science","volume":null,"pages":null},"PeriodicalIF":0.6,"publicationDate":"2024-03-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140366655","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Pollen Count Dynamics in Rapeseed Stamens in Early Spring 早春油菜花雄蕊中花粉数量的动态变化
IF 0.6 4区 农林科学
Journal of Apicultural Science Pub Date : 2023-12-01 DOI: 10.2478/jas-2023-0008
Zhijun Wei, Ran Liu, G. Ding, Yusuo Jiang, Jiaxing Huang
{"title":"Pollen Count Dynamics in Rapeseed Stamens in Early Spring","authors":"Zhijun Wei, Ran Liu, G. Ding, Yusuo Jiang, Jiaxing Huang","doi":"10.2478/jas-2023-0008","DOIUrl":"https://doi.org/10.2478/jas-2023-0008","url":null,"abstract":"Abstract Pollen contains vegetative and generative cells that influence the seed set. Accurate pollen assessments help understand the breeding biology of oilseed rape. In order to evaluate the number of pollen grains in a large number of samples, an effective method should be designed. A new method was established to evaluate the pollen counts of five oilseed rape cultivars. This method mainly relies on 5% sucrose + 0.1% agar solution to suspend the pollen and uses a standard curve corresponding to the absorbance and the number of pollen grains to estimate the number of pollen grains. The linear fit formula of this standard curve was precision, and the R2 value between the pollen count and absorbance reached 0.998. Furthermore, the variation in the pollen counts at three flowering stages and on two kinds of stamens was assessed. The pollen count per flower varied significantly among oilseed rape cultivars and flowering stages (GLM: p<0.001). Moreover, the number of pollen grains on long-stamen anthers was significantly higher than that on short-stamen anthers (GLM: p<0.001). In conclusion, we established a rapid, accurate method for quantifying pollen grains based on absorbance determined in a liquid suspension by light spectroscopy, which is a feasible method. In addition, the number of pollen grains under different physiological conditions also provides basic data for oilseed rape breeding.","PeriodicalId":14941,"journal":{"name":"Journal of Apicultural Science","volume":null,"pages":null},"PeriodicalIF":0.6,"publicationDate":"2023-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139021074","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Analysis of Diversity and Composition of Apis Cerana Gut Microbiome in Overwintering Period 蜜蜂越冬期肠道微生物组的多样性和组成分析
IF 0.6 4区 农林科学
Journal of Apicultural Science Pub Date : 2023-12-01 DOI: 10.2478/jas-2023-0009
Nannan Liu, Wenming Lv, Hongying Lv, Zhihao San, Yulan Piao, Fenglin Li
{"title":"Analysis of Diversity and Composition of Apis Cerana Gut Microbiome in Overwintering Period","authors":"Nannan Liu, Wenming Lv, Hongying Lv, Zhihao San, Yulan Piao, Fenglin Li","doi":"10.2478/jas-2023-0009","DOIUrl":"https://doi.org/10.2478/jas-2023-0009","url":null,"abstract":"Abstract Cold temperatures limit the survival and reproduction of honeybees. Therefore, successful overwintering is crucial for the survival of honeybee colonies and the pollination of flowering plants in the following spring. This study analyzed the gut microbiota of Apis cerana from Changbai Mountain during the overwintering period through 16S rDNA high-throughput sequencing. The analysis of microbial community composition showed that Gilliamella, Lactobacillus, Bifidobacterium and Snodgrassella were the core genera in the honeybee gut during the overwintering period. Moreover, alpha diversity analysis showed that the community diversity of the gut microbiota peaked in December. Beta diversity and LEfSe analysis showed that community composition was similar in December, January and February and that such beneficial bacteria as Snodgrassella, Acetobacteraceae, and Rhizobiales were differentially abundant during this period. The results of functional prediction analysis indicate that amino acid transport and metabolism, carbohydrate transport and metabolism, and the TCA cycle play an important role in overwintering. These results suggest that the dynamic changes in the gut microbiota of A. cerana during winter and mechanisms tolerate cold stress.","PeriodicalId":14941,"journal":{"name":"Journal of Apicultural Science","volume":null,"pages":null},"PeriodicalIF":0.6,"publicationDate":"2023-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139026475","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Adaptive Defence Strategies of the Stingless Bee, Tetragonula Iridipennis Smith. Against Nest Intruders in a Newly Divided Colony 无刺蜂 Tetragonula Iridipennis Smith.在新分化的蜂群中抵御巢穴入侵者的策略
IF 0.6 4区 农林科学
Journal of Apicultural Science Pub Date : 2023-12-01 DOI: 10.2478/jas-2023-0007
Udayakumar Amala, Venu Hunasikote Shamappa, Chandramanu Kandikere, Shylesha Arakalagud Nanjundaiah, Shivalingaswamy T. Maharudrappa
{"title":"Adaptive Defence Strategies of the Stingless Bee, Tetragonula Iridipennis Smith. Against Nest Intruders in a Newly Divided Colony","authors":"Udayakumar Amala, Venu Hunasikote Shamappa, Chandramanu Kandikere, Shylesha Arakalagud Nanjundaiah, Shivalingaswamy T. Maharudrappa","doi":"10.2478/jas-2023-0007","DOIUrl":"https://doi.org/10.2478/jas-2023-0007","url":null,"abstract":"Abstract A study was conducted to document nest intruders in newly divided colonies of stingless bees. The nature of the damage, the sequence of activities after colony division, and task allocation in the colony were studied. Three insect invaders - solitary resin bees (Megachile disjuncta, M. cephalotes), ants (Oecophylla smaragdina and Camponotus sp.) and spiders belonging to the family Salticidae and Lycosidae invaded the colonies during the earlier stages of division. The mean number of resin bees and ant invasions was significantly higher during the first week after division. Spiders invaded the hives and formed webs during the second week after division, resin and honey resources are robbed by resin bees and ants. Nest entrance closure was observed at 4.37±0.74 days after colony division guard bee activity from 6.13±1.24 days and foraging activity from 10.63±1.06 days after division. Observations on the task allocation framework after colony division indicated that 69% of bees were involved in hive entrance repair, 18% in colony architecture development, and 13% in tending to newly emerged bees from brood cells. The bees built a complex nest entrance, guarded activity and demonstrated nest closure behaviour in order to protect the nest against intruders. The results of the study are of prime importance for beekeepers to learn how to watch for hive intruders during colony division and to understand the adaptive defence mechanism to protect colonies.","PeriodicalId":14941,"journal":{"name":"Journal of Apicultural Science","volume":null,"pages":null},"PeriodicalIF":0.6,"publicationDate":"2023-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139018346","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Promoting Pollination and Pollinators in Farming 在农业生产中推广授粉和传粉媒介
IF 0.6 4区 农林科学
Journal of Apicultural Science Pub Date : 2023-12-01 DOI: 10.2478/jas-2023-0012
Peter Kevan, Susan Willis Chan
{"title":"Promoting Pollination and Pollinators in Farming","authors":"Peter Kevan, Susan Willis Chan","doi":"10.2478/jas-2023-0012","DOIUrl":"https://doi.org/10.2478/jas-2023-0012","url":null,"abstract":"","PeriodicalId":14941,"journal":{"name":"Journal of Apicultural Science","volume":null,"pages":null},"PeriodicalIF":0.6,"publicationDate":"2023-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139189972","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Toxicity of Insecticides with Different Modes of Action to Apis Mellifera Larvae (Hymenoptera, Apidae) 不同作用方式的杀虫剂对蜜蜂幼虫(膜翅目,蚜科)的毒性
IF 0.6 4区 农林科学
Journal of Apicultural Science Pub Date : 2023-12-01 DOI: 10.2478/jas-2023-0010
Mohammad Abdul Waseem, Meena Thakur
{"title":"Toxicity of Insecticides with Different Modes of Action to Apis Mellifera Larvae (Hymenoptera, Apidae)","authors":"Mohammad Abdul Waseem, Meena Thakur","doi":"10.2478/jas-2023-0010","DOIUrl":"https://doi.org/10.2478/jas-2023-0010","url":null,"abstract":"Abstract Pesticides are one of the elements that lead to the decline in honeybee populations. Honey bee while foraging collect nectar and pollen from various crops, can be exposed to pesticide residues, which may be transported into their colonies and consumed by the larvae. The objective of this study was to evaluate acute toxicity of fipronil and lambda-cyhalothrin, two commonly detected pesticides within beehives, on the larvae of Apis mellifera. The testes chemicals were given to three-day old larvae. Treatments were administered using a micropipette at the bottom of each chosen comb cell. Each treatment included sixty larvae per dosage and the experiment was repeated three times. Larvae were given only acetone in the control treatment. Fipronil and lambda-cyhalothrin were toxic to the larvae of A. mellifera with LD50 values of 0.163 and 0.83 μg/larvae, respectively after seven days of exposure. The maximum mortality of 81.35% was recorded with a dose of 0.38 μg/larvae in Fipronil, whereas in lambda-cyhalothrin, it was 94.23% with a dose of 1.70 μg/larvae.","PeriodicalId":14941,"journal":{"name":"Journal of Apicultural Science","volume":null,"pages":null},"PeriodicalIF":0.6,"publicationDate":"2023-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139018783","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
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