{"title":"Effect of Pollination by the Osmia Bicornis (syn. O. rufa) Bee on Fruit Set, Seed Set and Yield in Three Apple Cultivars","authors":"M. Fliszkiewicz, K. Giejdasz","doi":"10.2478/jas-2023-0011","DOIUrl":"https://doi.org/10.2478/jas-2023-0011","url":null,"abstract":"Abstract Our study tested the pollination effectiveness of the megachilid bee Osmia bicornis L. (syn. Osmia rufa) on three apple cultivars (cv.): Lobo, Piros and Champion. The following pollination treatments were compared for each cultivar: insect pollination - caged trees with Osmia bicornis (syn. Osmia rufa) bees; open pollination - uncaged trees; self-pollination - caged trees without bees. Pollination success was measured based on the percentage of fruit set, the fruit yield per tree (measured as number of fruits and total fruit weight) and the number of seeds per fruit. Generally, there was a higher percentage of initial fruit-set and fruits at harvest on open-pollinated trees than on caged trees without bees, with an intermediate value recorded for caged trees pollinated by O. bicornis (syn. O. rufa). However, the fruit set on trees pollinated by O. bicornis (syn. O. rufa) was high and ranged from 49 to 69%, depending on the cultivar. The cv. Lobo trees pollinated by O. bicornis (syn. O. rufa) and open-pollinated trees produced higher fruit yields than without bees. Furthermore, the number of seeds per fruit was higher. Fruit yield and the number of seeds per fruit were higher for trees pollinated by O. bicornis (syn. O. rufa) than for caged trees without insects but lower than for open pollination trees of cv. Piros and cv. Lobo.","PeriodicalId":14941,"journal":{"name":"Journal of Apicultural Science","volume":null,"pages":null},"PeriodicalIF":0.6,"publicationDate":"2023-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139014676","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Mateusz Plotnik, P. Bieńkowski, E. Berbeć, A. Murawska, Krzysztof Latarowski, P. Migdał
{"title":"Influence of Electromagnetic Field with Frequency of 50 Hz in form of Doses on Selected Biochemical Markers of Honey Bee","authors":"Mateusz Plotnik, P. Bieńkowski, E. Berbeć, A. Murawska, Krzysztof Latarowski, P. Migdał","doi":"10.2478/jas-2023-0003","DOIUrl":"https://doi.org/10.2478/jas-2023-0003","url":null,"abstract":"Abstract The honey bee has a significant environmental and economic impact. While collecting food or water, bees are exposed to negative physical and chemical factors that lead to physiological and behavioral changes and, consequently, even death. Along with the development of technology and communication, electromagnetic fields produced by artificial emitters have begun to have an impact on the environment. The aim of the study was to check whether the electromagnetic field also impacts antioxidant enzymes functioning in the honey bee's organism. The study was conducted under laboratory conditions, and one-day and seven-day-old honey bee workers were used in the experiment. Honey bee workers were exposed to an electromagnetic field with a frequency of 50Hz and variable intensity in the range of 1–10 kV/m. Immediately after the end of the exposure, hemolymph was collected from the bees for biochemical analysis. The results of the research did not show clearly whether changes in the activity of biochemical markers were affected by the time spent in the electromagnetic field or its intensity but did show that there was a difference in physiology between one-day-old and seven-day-old bees.","PeriodicalId":14941,"journal":{"name":"Journal of Apicultural Science","volume":null,"pages":null},"PeriodicalIF":0.6,"publicationDate":"2023-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42125642","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Y. Tumbarski, M. Todorova, M. Topuzova, Gabriela Gineva, V. Yanakieva, Ivan Georgiev Ivanov, N. Petkova
{"title":"Comparative Study on Physicochemical, Antioxidant and Antimicrobial Properties of Propolis Collected from Different Regions of Bulgaria","authors":"Y. Tumbarski, M. Todorova, M. Topuzova, Gabriela Gineva, V. Yanakieva, Ivan Georgiev Ivanov, N. Petkova","doi":"10.2478/jas-2023-0004","DOIUrl":"https://doi.org/10.2478/jas-2023-0004","url":null,"abstract":"Abstract Propolis (bee glue), a valuable biological mixture produced by European honey bees (Apis mellifera L.), is widely used as a remedy in traditional and alternative medicine, as an effective food biopreservative and as a nutritional value enhancer. The present study aimed to investigate eighty propolis samples collected from all twenty-eight districts of Bulgaria in the period of 2020–2022 in order to determine their physicochemical properties as well as their antioxidant and antimicrobial potential. The raw propolis samples exhibited brown or green colour depending on the climatic zone from which they are collected and pH values from 4.82 to 5.87, while the moisture content ranged between 0.98% and 2.97%. The total phenolics content (TPC) and total flavonoids content (TFC) varied from 63.14 mg GAE/g to 737.27 mg GAE/g, and from 29.22 mg QE/g to 234.17 mg QE/g, respectively. The propolis samples demonstrated significant antioxidant potential determined by DPPH and FRAP methods, from 18.56 mM TE/g to 1598.66 mM TE/g and from 82.28 mM TE/g to 1208.81 mM TE/g, respectively, whose values showed a positive correlation (r2) with the TPC and TFC. The results from the antimicrobial screening revealed that the methanolic propolis extracts in concentration of 20 mg/ml exhibited the highest inhibitory effect on the Gram-positive bacteria such as Micrococcus luteus 2YC-YT, Bacillus subtilis ATCC 6633, Staphylococcus aureus ATCC 25923, Listeria monocytogenes NBIMCC 8632 and Listeria innocua ATCC 33090.","PeriodicalId":14941,"journal":{"name":"Journal of Apicultural Science","volume":null,"pages":null},"PeriodicalIF":0.6,"publicationDate":"2023-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43567607","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Prevalence and Molecular Characterization of Deformed Wing, Acute Bee Paralysis and Black Queen Cell Viruses Infecting Honey Bees and Varroa Mites","authors":"Songül Aydın, H. Oksal","doi":"10.2478/jas-2023-0006","DOIUrl":"https://doi.org/10.2478/jas-2023-0006","url":null,"abstract":"Abstract Surveys were conducted in Malatya and Elazığ provinces of Eastern Türkiye between 2018–2019 to detect the presence and distribution of Deformed wing virus (DWV), Acute bee paralysis virus (ABPV) and Black queen cell virus (BQCV) infecting honey bees and Varroa mites. Thirty Varroa mites feeding on honey bees and 147 honey bees were collected from twenty-five apiaries of Malatya and fifteen apiaries of Elazığ. The collected samples were subjected to reverse transcriptase-polymerase chain reaction. All tested viruses were found to be present in the samples singly or as mixed infections. The prevalence of the viruses were 23.81%, 12.93%, and 10.20% for DWV, ABPV and BQCV, respectively. Five out of thirty Varroa mites tested for the viruses were found to be infected only with DWV. Randomly chosen sequences of each detected virus’s partial polyprotein gene region were registered in GenBank under the accession numbers OP805878, OP805879, OP805880, OP805887, OP805888, OP805889, OP805890, OP805891 (DWV), OP805881, OP805882, OP805883, OP805884 (ABPV) and OP805885, OP805886 (BQCV). The phylogenetic tree of the viral isolates were compared with the world isolates. DWV isolates were found to be closely related to the UK, Lebanon, Türkiye, France, Germany and Israel isolates, whereas ABPV isolates were related to Türkiye, South Africa, Slovenia, Serbia, France, Hungary, Syria and USA isolates and BQCV isolates were related to China, South Korea, Czech Republic, France, Hungary, Poland, South Africa, Australia and Sweden isolates. The presence of DWV, ABPV and BQCV infections in Malatya and Elazığ provinces of Türkiye is revealed for the first time in this study.","PeriodicalId":14941,"journal":{"name":"Journal of Apicultural Science","volume":null,"pages":null},"PeriodicalIF":0.6,"publicationDate":"2023-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43099341","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Ran Liu, Zhijun Wei, Cheng Liang, Yong-Mei Huang, G. Ding, Yusuo Jiang, Jiaxing Huang
{"title":"Relationships between Flower Opening Time, Environmental Conditions, Corolla Opening Size and Nectar Production in Five Winter Oilseed Rape (Brassica napus L.) Cultivars in China","authors":"Ran Liu, Zhijun Wei, Cheng Liang, Yong-Mei Huang, G. Ding, Yusuo Jiang, Jiaxing Huang","doi":"10.2478/jas-2023-0005","DOIUrl":"https://doi.org/10.2478/jas-2023-0005","url":null,"abstract":"Abstract Oilseed rape is an economically important crop and provides a good nectar resource. In this study, the nectar secretion characteristics of five winter oilseed rape (Brassica napus L.) cultivars in China were examined throughout the daytime. The correlations of corolla opening size, air temperature and relative humidity with nectar production were also determined. Different daily patterns of nectar secretion were detected in flowers opening at different times of the day, and similar trends were observed among cultivars. The nectar volume and nectar sugar concentration in flowers that opened before 9:00 peaked on the first day. The nectar volume of flowers that opened at 11:00 peaked on the second day, and the nectar sugar concentration peaked on the first day. The total nectar yield of flowers opening before 9:00 (4.422–5.265 μl) was lower than that of flowers opening at 11:00 (7.982–10.646 μl). The average nectar sugar concentration of flowers opening before 9:00 (18.4–23.3%) was higher than that of flowers opening at 11:00 (15.3–17.5%). The nectar volume was positively correlated with air temperature and relative humidity, while the nectar sugar concentration was positively correlated with air temperature and negatively correlated with relative humidity. The nectar volume and nectar sugar concentration were positively correlated with corolla opening size. The glucose/fructose ratio ranged from 0.89 to 1.44. These results will contribute to a better understanding of the nectar secretion patterns of B. napus L. and will support further studies assessing the melliferous potential of B. napus L. and the temporal dynamics of plant-pollinator interactions.","PeriodicalId":14941,"journal":{"name":"Journal of Apicultural Science","volume":null,"pages":null},"PeriodicalIF":0.6,"publicationDate":"2023-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43817509","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
M. Tomczyk, Julita Kusibab, Alicja Zachara, M. Dżugan
{"title":"Assessment of Mercury Level in Local Polish and Imported Honeys with Use of Direct Mercury Analyzer","authors":"M. Tomczyk, Julita Kusibab, Alicja Zachara, M. Dżugan","doi":"10.2478/jas-2023-0001","DOIUrl":"https://doi.org/10.2478/jas-2023-0001","url":null,"abstract":"Abstract Mercury is a toxic metal which causes several serious health effects including kidney damage, anxiety depression and peripheral neuropathy, and because of its high volatility assessment in solid samples is problematic. In this study, forty-five samples of local Polish and imported honeys were analyzed by Direct Mercury Analyzer (DMA) for the determination of mercury content. The honey contamination was assessed regarding honey variety and country of origin. Hg level in all tested samples was found to not exceed the 0.81 μg/kg which was below 10% of the applicable law UE limit. Moreover, no statistically significant differences were found in the variety or geographical origin. The study concluded that the Direct Mercury Analyzer (DMA) instrument proved to be an excellent tool for screening mercury in honeys because of its simplicity, rapidity, low detection limit, accuracy and precision and no sample preparation.","PeriodicalId":14941,"journal":{"name":"Journal of Apicultural Science","volume":null,"pages":null},"PeriodicalIF":0.6,"publicationDate":"2023-05-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48964733","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Health Status of Honeybee Colonies Differing in Genetic Intra-Colonial Diversity","authors":"D. Gerula","doi":"10.2478/jas-2023-0002","DOIUrl":"https://doi.org/10.2478/jas-2023-0002","url":null,"abstract":"Abstract Two different levels of diversity within a colony were compared for the prevalence of pathogens and diseases. Lower genetic diversity was obtained in the colonies in which the queens were inseminated with semen collected from drones originating from a single colony, while greater was obtained in the colonies with queens inseminated with semen from drones of thirty different colonies. Bees were tested for Varroa destructor infestation, microsporidia Vairimorpha spp. infection, acute bee paralysis virus (ABPV) and deformed wing virus (DWV). Colonies with a greater genetic diversity of workers in colonies were more infested with Varroa mites than genetically uniform colonies. Varroa infestation was not found to be associated directly with the weakening of bee colonies after winter. The two experimental groups had a similar number of colonies infected with Vairimorpha, and viruses. Intensity of Varroa infestation and Vairimorpha infection did not significantly affect the overwintering of bee colonies. Colonies in which DWV was detected significantly weakened during overwintering.","PeriodicalId":14941,"journal":{"name":"Journal of Apicultural Science","volume":null,"pages":null},"PeriodicalIF":0.6,"publicationDate":"2023-05-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41547376","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Washboarding: Are Man-Managed Honeybees Performing a Vestigial Activity Fostered by Cryptogams?","authors":"M. Giovanetti, C. Máguas, S. Munzi","doi":"10.2478/jas-2022-0016","DOIUrl":"https://doi.org/10.2478/jas-2022-0016","url":null,"abstract":"Abstract A common view is that honeybees are mostly managed by beekeepers for commercial purposes or as a hobby, especially in Europe. This misconception is probably due to the lack of systematic studies on wild colonies of honeybees in Europe in comparison to other regions of the world. Since we are used to considering this species as “domesticated”, we may be induced to disregard activities not distinctly linked with colony survival, reproduction, or productivity. Washboarding is one of them in which the entire colony stops resource collection activities; numerous individual bees assemble on the front side of the hive and perform repetitive movements back and forth. They are curiously synchronised but apparently without a scope. In this exploratory work, we carried out a literature review of available, mostly grey, literature. Assuming this behaviour may be linked to cavity-nesting and to tree trunks or rocks being rough surfaces hosting various cryptogams, we performed preliminary observations and manipulative experiments. From our survey, we depict that washboarding is frequently reported in grey literature/beekeepers’ reports, but rarely mentioned in scientific literature. Beekeepers who responded to a designed questionnaire observed this behaviour in various ecological situations, with no trend emerging. Our preliminary experiment of placing cryptogams in front of managed hives resulted in honeybees removing lichens (foliose types) or covering with propolis, and all species were affected. Further research is needed to clarify if lichens are removed because of their chemical compounds, because of hosting potentially toxic microorganisms, or collected as resources.","PeriodicalId":14941,"journal":{"name":"Journal of Apicultural Science","volume":null,"pages":null},"PeriodicalIF":0.6,"publicationDate":"2022-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48925235","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Static Water Vapor Sorption Properties of Honey","authors":"K. Arslan, M. Turhan","doi":"10.2478/jas-2022-0010","DOIUrl":"https://doi.org/10.2478/jas-2022-0010","url":null,"abstract":"Abstract Static sorption properties of pine honey (PH) and citrus honey (CH) were studied at 20, 30 and 40°C. Yeast formation was observed on the surface of honeys at water activity (aw)>0.7 at all temperatures. Visible yeast formation (YF) took place earlier in PH than in CH under the same conditions due to its characteristic higher pH and lower monosacchride content. The temperature was insignificantly effective on YF (p>0.05). The honeys exhibited a sorption isotherm (SI) in the shape of a “J”. Their SIs exhibited desorption and adsorption at aw<0.7 and aw>0.7, respectively and the desorption part was almost linear. Aw=0.7 emerged as a border between the absence and presence of YF and between the desorption and adsorption. The SIs of honeys were insignificantly affected by temperature (p>0.05). PH had a significantly lower SI than that of CH (p≤0.05) due to its characteristic lower monosacchride content. GAB equation exhibited a good fit to the honeys' SIs. Sorption heat vs equilibrium moisture content (EMC) revealed monolayer, multilayer and loosely bound free water regions in the SIs of PH and CH.","PeriodicalId":14941,"journal":{"name":"Journal of Apicultural Science","volume":null,"pages":null},"PeriodicalIF":0.6,"publicationDate":"2022-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41971446","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Molecular Diagnostic Survey of Honey Bee, Apis mellifera L., Pathogens and Parasites from Arkansas, USA","authors":"D. Cleary, Allen L. Szalanski","doi":"10.2478/jas-2022-0014","DOIUrl":"https://doi.org/10.2478/jas-2022-0014","url":null,"abstract":"Abstract Managed honey bee populations have fluctuated over the past several decades in the U.S. While a single factor has not been identified for these losses, the interaction between multiple biotic and abiotic stressors have been suggested to be responsible. Of major concern are several invasive parasite and pathogen species as well as colony management. A single honey bee colony often suffers from multiple harmful agents, that may act synergistically and cause greater declines in bee health. We conducted a survey to detect known and lesser-known honey bee parasites and pathogens. While previous research has primarily focused on commercially managed colonies, research is limited to pertaining parasite and pathogen prevalence in hobbyist managed colonies. Molecular diagnostics were used to screen 541 Arkansas (AR) honey bee colonies from 107 hobbyist beekeepers for eight A. mellifera parasite and pathogen species. Colony samples were obtained between 2015–2016 and represented forty-seven of the seventy-five AR counties. Vairimorpha ceranae (11.6% occurrence) and parasite Varroa destructor (49.4% occurrence) were relatively common in AR hobbyist colonies. Interestingly, the lesser-studied pathogenic trypanosome species, Lotmaria passim, was detected in 11.3% of the colonies and widespread in twenty of the forty-seven counties sampled. None of the honey bee pathogens Vairimorpha apis, Spiroplasma apis, S. melliferum, Crithidia mellificae, or the parasitic phorid fly, Apocephalus borealis, were detected in the colonies sampled. This study provides an extensive assessment of the parasite and pathogen species occurring at the AR state-level in hobbyist-managed honey bee colonies.","PeriodicalId":14941,"journal":{"name":"Journal of Apicultural Science","volume":null,"pages":null},"PeriodicalIF":0.6,"publicationDate":"2022-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46438593","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}