Journal of Apicultural Science最新文献_第10页

Effect of Fermentation on Structural Characteristics and Bioactive Compounds of Bee-Pollen Based Food 发酵对蜂花粉基食品结构特性及生物活性成分的影响

IF 0.6 4区农林科学

Journal of Apicultural Science Pub Date : 2019-12-01 DOI: 10.2478/jas-2019-0016

C. Zuluaga-Domínguez, M. Quicazán

{"title":"Effect of Fermentation on Structural Characteristics and Bioactive Compounds of Bee-Pollen Based Food","authors":"C. Zuluaga-Domínguez, M. Quicazán","doi":"10.2478/jas-2019-0016","DOIUrl":"https://doi.org/10.2478/jas-2019-0016","url":null,"abstract":"Abstract Bee-pollen is a product of the hive which has had a growth in consumption in recent years due to the recognition of its nutritional and bioactive potential. However, several reports have shown that the external structure of the grain limits the absorption of nutrients in the human gastrointestinal tract. A structural modification could be achieved through fermentative processes, favoring the release of compounds found inside this food, in addition to obtaining a product with potential probiotic characteristics. The objective of this work was to evaluate how fermentation through the inclusion of yeasts of the species Saccharomyces cerevisiae, bacteria of species Lactobacillus plantarum or a commercial culture Choozit® affeccted such parameters as Scanning Electron Microscopy (SEM), Differential Scanning Calorimetry (DSC), phenolic compounds, flavonoids and antioxidant activity. The results found that the use of consortia between yeast and lactic acid bacteria significantly increased in such characteristics as total phenolics and antioxidant activity by 31% and 39% respectively. The analysis by DSC showed an increase in the heat flow of the fermented products compared to fresh bee-pollen, which could indicate structural modification caused by the activity of microorganisms, a fact made visible through micrographs obtained by Scanning Electron Microscopy.","PeriodicalId":14941,"journal":{"name":"Journal of Apicultural Science","volume":"63 1","pages":"209 - 222"},"PeriodicalIF":0.6,"publicationDate":"2019-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45814274","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 12

Bee Pollen Extracts as Potential Antioxidants and Inhibitors of α-Amylase and α-Glucosidase Enzymes In Vitro Assessment 蜂花粉提取物作为潜在抗氧化剂和α-淀粉酶和α-葡萄糖苷酶抑制剂的体外评价

IF 0.6 4区农林科学

Journal of Apicultural Science Pub Date : 2019-12-01 DOI: 10.2478/jas-2019-0020

O. Daudu

{"title":"Bee Pollen Extracts as Potential Antioxidants and Inhibitors of α-Amylase and α-Glucosidase Enzymes In Vitro Assessment","authors":"O. Daudu","doi":"10.2478/jas-2019-0020","DOIUrl":"https://doi.org/10.2478/jas-2019-0020","url":null,"abstract":"Abstract The study was conducted to determine the antioxidant and anti-diabetic properties of bee pollen. The phenol content of extracts was 1.43, 2.04, 2.10 and 1.79 mg gallic acid g-1, respectively. Total flavonoid content was 0.78, 1.39, 0.86 and 0.79 mg gallic acid g-1 respectively. Bee pollen extracts have the potential to scavenge free radicals and lower blood glucose due to the presence of phenols and flavonoids. The aqueous-ethanol extract had the lowest IC50 for HRS and TAC, 0.53mg/mL and 0.25mg/mL respectively, which depicts that this extract alleviates the destructive effect of hydroxyl radicals. Methanol extract had the lowest IC50 0.21 mg/mL) for DPPH inhibition, hence it was able to scavenge the DPPH radicals. Ethanol extract had the lowest IC50 (0.04 mg/mL) for 2, 2-azino-bis (3-ethylbenzothiazoline-6-sulfonic acid inhibition, hence it can protect living cells from ABTS cations. The aqueous-ethanol extract displayed higher inhibition of α-amylase (4.51 mg/mL) while the aqueous extract exhibited a higher inhibition of α-glucosidase (0.60 mg/mL), which slows down the breakdown of disaccharides and reduces sugar buildup in the bloodstream. Bee pollen has potential antioxidative and antidiabetic activity.","PeriodicalId":14941,"journal":{"name":"Journal of Apicultural Science","volume":"63 1","pages":"315 - 325"},"PeriodicalIF":0.6,"publicationDate":"2019-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43468956","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 17

Comparison of Predicted Amino Acid Sequences of Allatotropin/Allatostatin Receptors from Solitary to Eusocial Bee Species (Hymenoptera, Apoidea) 孤居与合居蜜蜂allattropin /Allatostatin受体预测氨基酸序列的比较(膜翅目，apo总科)

IF 0.6 4区农林科学

Journal of Apicultural Science Pub Date : 2019-12-01 DOI: 10.2478/jas-2019-0021

Huipeng Yang, Jie Wu

{"title":"Comparison of Predicted Amino Acid Sequences of Allatotropin/Allatostatin Receptors from Solitary to Eusocial Bee Species (Hymenoptera, Apoidea)","authors":"Huipeng Yang, Jie Wu","doi":"10.2478/jas-2019-0021","DOIUrl":"https://doi.org/10.2478/jas-2019-0021","url":null,"abstract":"Abstract An increasingly amount of evidence supports that the evolution of eusociality is accompanies by shifts in ancient molecular and physiological pathways. The juvenile hormone, one of the most important hormones in the post-embryonic development of insects, attracts the most attention in the context of social organization. Allatoregulatory neuropeptides (Allatotropin, Allatostatin-A and Allatostatin-C) are known to regulate juvenile hormone synthesis and release in insects. In order to clarify the transitions of juvenile hormone synthesis involved in eusocial evolution, the substitutions of amino acid residues and the complexity of post-translational modifications in allatoregulatory neuropeptide receptors were characterized. Both allatotropin and allatostatin receptors are identified in all examined bee species regardless if they are solitary or eusocial. Although the amino acid sequences are highly conserved, phylogenetic results are consistent with the eusocial status. The abundance of predicted post-translational modifications correlates with social complexity except for that in allatostatin-C receptors. Even though the consequences of these specific amino acid substitutions and various post-translational modification complexity have not been studied, they likely contribute to the localizing, binding and coupling characteristics of the receptor groups.","PeriodicalId":14941,"journal":{"name":"Journal of Apicultural Science","volume":"63 1","pages":"267 - 279"},"PeriodicalIF":0.6,"publicationDate":"2019-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43503596","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Phylogenetic Relationships of Russian Far-East Apis cerana with Other North Asian Populations 俄罗斯远东蜜蜂与其他北亚种群的系统发育关系

IF 0.6 4区农林科学

Journal of Apicultural Science Pub Date : 2019-12-01 DOI: 10.2478/jas-2019-0024

R. Ilyasov, Han Gi Youn, Myeong-lyeol Lee, K. Kim, M. Proshchalykin, A. Lelej, J. Takahashi, H. Kwon

{"title":"Phylogenetic Relationships of Russian Far-East Apis cerana with Other North Asian Populations","authors":"R. Ilyasov, Han Gi Youn, Myeong-lyeol Lee, K. Kim, M. Proshchalykin, A. Lelej, J. Takahashi, H. Kwon","doi":"10.2478/jas-2019-0024","DOIUrl":"https://doi.org/10.2478/jas-2019-0024","url":null,"abstract":"Abstract Apis cerana Fabricius, 1793 is the eastern honeybee species distributed throughout Asia from the tropical climate in the southern part to the temperate climate in the northern part. We sequenced and annotated the complete mitochondrial DNA (mtDNA) of A. cerana from Vladivostok, Primorsky Krai of the Russian Far East and uploaded it to the database GenBank (AP018450). MtDNA sequence has 15,919 bp length, AT-content 84% and GC-content 16% and contains 22 tRNA genes, 13 protein-coding genes, two ribosomal RNA genes, one AT-rich region and four non-coding intergenic regions (NC1-4). All proteincoding genes start with ATT and ATG codons, except for ATC, the start codon of the ATP8 gene, which and stop with the common stop codons TAA and TAG. A comparative analysis of complete mtDNA of A. cerana from China, Indonesia, Korea, Malaysia, Russia, Taiwan, Thailand, Vietnam, and Japan found that the Russian Far East Apis cerana differed from others on the subspecies level. Based on the comparative analysis of complete mtDNA (~16,000 bp), nuclear DNA (nDNA) gene Vitellogenin (VG) (~4,100 bp) and morphological measurements (six parameters), we assumed that the Russian Far-East A. cerana can be a distinct northern Asia population and can be described as a separate unique subspecies of A. c. ussuriensis subsp. nov. A. c. koreana subsp. nov. is also validated and described as a new subspecies.","PeriodicalId":14941,"journal":{"name":"Journal of Apicultural Science","volume":"63 1","pages":"289 - 314"},"PeriodicalIF":0.6,"publicationDate":"2019-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"69215332","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 16

Prospects and Validity of Laboratory Cage Tests Conducted in Honeybee Research Part one: Main Directions of Use of Laboratory Cage Tests in Honeybee Research 蜂箱试验在蜜蜂研究中的应用前景与有效性第一部分：蜂箱试验的主要应用方向

IF 0.6 4区农林科学

Journal of Apicultural Science Pub Date : 2019-12-01 DOI: 10.2478/jas-2019-0026

P. Dziechciarz, G. Borsuk, K. Olszewski

{"title":"Prospects and Validity of Laboratory Cage Tests Conducted in Honeybee Research Part one: Main Directions of Use of Laboratory Cage Tests in Honeybee Research","authors":"P. Dziechciarz, G. Borsuk, K. Olszewski","doi":"10.2478/jas-2019-0026","DOIUrl":"https://doi.org/10.2478/jas-2019-0026","url":null,"abstract":"Abstract For more than forty years, laboratory cage tests (LCTs) have been used in honeybee research. These tests facilitate the control of elements of the environment present in the laboratory, which allows the minimization of the impact of the changing environmental conditions and conditions in the bee colony nest on the studied features. Therefore, it is possible to estimate the content of the genetic component of a particular trait and to assess the impact of a specific substance or tested agent on the traits of bees. An advantage of LCTs is the possibility of conducting easier and cheaper research than in the case of apiary experiments. LCTs allow a significant reduction in the number of required bees/colonies, investigations of a winter bee generation and individual treatment of a single bee. One of the first research directions in which LCTs were used was the instantaneous assessment of the honey yield. Due to discrepancies between LCT assessment and results of apiary productivity, LCTs are currently not a standard tool for the assessment and improvement of bee functional traits despite such undoubted advantages as reducing the time and cost of the evaluation. Moreover, LCTs are being used to assess the genetic and environmental conditions of workers’ lifespan and are a standard method for assessing the impact of pesticides on bees.","PeriodicalId":14941,"journal":{"name":"Journal of Apicultural Science","volume":"63 1","pages":"201 - 207"},"PeriodicalIF":0.6,"publicationDate":"2019-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49603739","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 3

Broad Geographic and Host Distribution of Apis mellifera Filamentous Virus in South American Native Bees 蜂蜡丝状病毒在南美本土蜜蜂中的广泛地理和宿主分布

IF 0.6 4区农林科学

Journal of Apicultural Science Pub Date : 2019-12-01 DOI: 10.2478/jas-2019-0025

S. Quintana, G. D. de Landa, P. Revainera, F. Meroi, L. Porrini, V. di Gerónimo, C. Brasesco, S. Plischuk, M. Eguaras, M. Maggi

引用次数: 4

Dynamics of Nosema apis and Nosema ceranae Co-Infection Seasonally in Honey Bee (Apis mellifera L.) Colonies 蜜蜂（apis mellifera L.）群落中蜜蜂和蜜蜂共同感染的季节动态

IF 0.6 4区农林科学

Journal of Apicultural Science Pub Date : 2019-06-01 DOI: 10.2478/jas-2019-0001

A. Özkırım, A. Schiesser, Nevin Keskin

{"title":"Dynamics of Nosema apis and Nosema ceranae Co-Infection Seasonally in Honey Bee (Apis mellifera L.) Colonies","authors":"A. Özkırım, A. Schiesser, Nevin Keskin","doi":"10.2478/jas-2019-0001","DOIUrl":"https://doi.org/10.2478/jas-2019-0001","url":null,"abstract":"Abstract Nosema apis is a pathogen spesific for the European honeybee, Apis mellifera L., while Nosema ceranae is specific for the Asian honeybee, Apis cerana. Turkey provides different environmental and host conditions for both Nosema species. The aim of the study is to determine the dynamic of N. cerenae and N. apis seasonal infection. A number of samples were collected from different apiaries between 2009-2016 years. The samples were kept at −20°C in the laboratory. Light microscopy was used for spore counting and molecular techniques were used to identify the Nosema species. The results showed that winter season had an impact on the type of Nosema as well as on infection rates. The number of N. ceranae spores decreases significantly at low temperatures (≤ 5°C). The winter period was found to be the main factor affecting nosema infection level and dominancy of Nosema ceranae. Furthermore, co-infection of both species is an indicator of the dynamics of N. apis and N. ceranae. This study suggests, that there is a dynamic prevalence among the Nosema species depending of the average winter temperature and not a replacement of N. apis by N. ceranae.","PeriodicalId":14941,"journal":{"name":"Journal of Apicultural Science","volume":"63 1","pages":"41 - 48"},"PeriodicalIF":0.6,"publicationDate":"2019-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47559270","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 9

Antioxidative Properties of Selected Polish Honeys 精选波兰蜂蜜的抗氧化性能

IF 0.6 4区农林科学

Journal of Apicultural Science Pub Date : 2019-06-01 DOI: 10.2478/jas-2019-0003

P. Piszcz, B. Głód

{"title":"Antioxidative Properties of Selected Polish Honeys","authors":"P. Piszcz, B. Głód","doi":"10.2478/jas-2019-0003","DOIUrl":"https://doi.org/10.2478/jas-2019-0003","url":null,"abstract":"Abstract The antioxidative activities of honeys collected in Poland were screened. The total antioxidant potential (TAP) provides more information about the system than the determination of individual antioxidant. TAP is proportional to the sum of products of concentrations of all antioxidants in the sample and their antioxidant powers (rate constants). To measure, compare and correlate TAPs, we used techniques which we had recently elaborated (i) related to hydroxyl radicals, (ii) RP-HPLC measurements with amperometric detection and (iii) differential pulsed voltammetry (DPV). They were correlated with techniques already described in the literature (i) related to the DPPH radicals, (ii) the total content of phenolic compounds and (iii) color intensity. All assays revealed the following order of obtained TAP values: buckwheat > honeydew > linden > multi-flower> acacia honey. Correlations were found between results obtained using different techniques. There was also a significant correlation with the results obtained by authors using other measurement techniques. Differences in antioxidant properties among individual honeys are inversely proportional to the strength of the radicals in relation to which measurements were performed. This is due to the fact that strong radicals react not only with strong antioxidants but also with weak ones, which are much more common. The darker honeys were also observed to be characterized by higher TAP values.","PeriodicalId":14941,"journal":{"name":"Journal of Apicultural Science","volume":"63 1","pages":"81 - 91"},"PeriodicalIF":0.6,"publicationDate":"2019-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42328785","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 8

Geographic Range and Nest Architecture of Cephalotrigona Capitata Smith, 1854 (Apidae: Meliponini) in the State of Bahia, Northeastern Brazil 1854年，巴西东北部巴伊亚州的头齿鹬的地理范围和巢结构(翅目:头齿鹬科)

IF 0.6 4区农林科学

Journal of Apicultural Science Pub Date : 2019-06-01 DOI: 10.2478/jas-2019-0006

R. M. Alves, A. M. Waldschmidt, J. F. Paixão, D. R. Santos, C. Carvalho

{"title":"Geographic Range and Nest Architecture of Cephalotrigona Capitata Smith, 1854 (Apidae: Meliponini) in the State of Bahia, Northeastern Brazil","authors":"R. M. Alves, A. M. Waldschmidt, J. F. Paixão, D. R. Santos, C. Carvalho","doi":"10.2478/jas-2019-0006","DOIUrl":"https://doi.org/10.2478/jas-2019-0006","url":null,"abstract":"Abstract The bees of the genus Cephalotrigona (locally known as “mombucas”) play a key role in natural environments but their bioecological features, required to design proper management and conservation strategies, are scarce in most species. Thus, the goal of the present study was to map the occurrence sites of C. capitata in the state of Bahia, north-eastern Brazil, and to provide useful information about nest architecture to their technical management. This species was recorded in fifteen municipalities in Bahia, totaling forty-one nests. The range of C. capitata varied from locations at sea level to seasonal ombrophilous forests at an altitude of 600 m high. The nests were built in trees with a mean diameter of 19.8 ±3.0 cm. The nest architecture was similar to that reported in other stingless bee species, with a variation in analyzed parameters. The thermoregulation was more efficient in highly populated boxes. The present results can be used for the conservation and management of this species, which represents a potential source of income for local farmers.","PeriodicalId":14941,"journal":{"name":"Journal of Apicultural Science","volume":"63 1","pages":"49 - 60"},"PeriodicalIF":0.6,"publicationDate":"2019-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44683084","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Maternal Inheritance in Hybrids of Three Honey Bee Subspecies 三种蜜蜂亚种杂交后代的母系遗传

IF 0.6 4区农林科学

Journal of Apicultural Science Pub Date : 2019-06-01 DOI: 10.2478/jas-2019-0010

Paweł Węgrzynowicz, D. Gerula, A. Tofilski, Beata Panasiuk, M. Bieńkowska

{"title":"Maternal Inheritance in Hybrids of Three Honey Bee Subspecies","authors":"Paweł Węgrzynowicz, D. Gerula, A. Tofilski, Beata Panasiuk, M. Bieńkowska","doi":"10.2478/jas-2019-0010","DOIUrl":"https://doi.org/10.2478/jas-2019-0010","url":null,"abstract":"Abstract The identification of honey bee (Apis mellifera) subspecies is often based on the measurements of workers’ fore-wings. The interpretation of the measurements can be difficult because the phenotype of workers is affected by both genetic and environmental factors. Moreover, it is not clear how the phenotype is affected by maternal inheritance. We have used the methodology of geometric morphometrics to verify if hybrids of honey bee subspecies and their backcrosses are more similar to either the father or mother colony. The comparison was based on fore-wing venation of three honey bee subspecies: A. m. carnica, A. m. caucasica, A. m. mellifera. First generation hybrids and backcrosses of those subspecies were obtained through instrumental insemination. Workers of the hybrids were compared with their parental colonies. The shape of wing venation was more similar to the maternal than to parental colony. This phenomenon was particularly visible in first generation of hybrids but it was also present in backcrosses. There were also symptoms of genetic dominance of some subspecies but this effect interacted with maternal inheritance and was difficult to interpret.","PeriodicalId":14941,"journal":{"name":"Journal of Apicultural Science","volume":"63 1","pages":"131 - 138"},"PeriodicalIF":0.6,"publicationDate":"2019-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44753946","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 6