Journal of Analytical Sciences, Methods and Instrumentation最新文献_第9页

A Rapid Method for Determination of Some Phenolic Acids in Brazilian Tropical Fruits of Mangaba ( Hancornia speciosa Gomes ) and Umbu ( Spondias tuberosa Arruda Camara ) by UPLC UPLC快速测定巴西热带水果Mangaba (Hancornia speciosa Gomes)和umu (Spondias tuberosa Arruda Camara)中酚酸的含量

Journal of Analytical Sciences, Methods and Instrumentation Pub Date : 2013-09-23 DOI: 10.4236/JASMI.2013.33A001

E. D. B. Gomes, S. A. Ramalho, N. C. Gualberto, Rita Miranda, Nisha Nigam, N. Narain

{"title":"A Rapid Method for Determination of Some Phenolic Acids in Brazilian Tropical Fruits of Mangaba ( Hancornia speciosa Gomes ) and Umbu ( Spondias tuberosa Arruda Camara ) by UPLC","authors":"E. D. B. Gomes, S. A. Ramalho, N. C. Gualberto, Rita Miranda, Nisha Nigam, N. Narain","doi":"10.4236/JASMI.2013.33A001","DOIUrl":"https://doi.org/10.4236/JASMI.2013.33A001","url":null,"abstract":"A rapid chromatographic \u0000method for the determination of six phenolic acids (chlorogenic, ferulic, \u0000gallic, p-coumaric, protocatechuic \u0000and vanillic acids) by ultra performance liquid chromatography \u0000(UPLC), was developed and applied for Brazilian tropical fruits mangaba \u0000(Hancornia speciosa Gomes) and umbu (Spondias tuberosa Arruda Camara). \u0000A multivariate statistical experimental design was employed to optimize \u0000analytical conditions (solvent A, solvent B concentrations and flow rates). \u0000Samples were cleaned-up by solid-phase extraction (SPE) with different solvents \u0000(methanol and acetone) employing SPE cartridges (amine and octadecyl-silane). \u0000The method using dihydrogen potassium phosphate 5 mmoL as solvent A and 8% acetonitrile \u0000as solvent B presented limits of detection varying from 14 to 94 ng.mL, limits of quantification from 39 to 277 ng.mL-1 with 2 μL of injection volume while total run \u0000time for all six compounds was only 9.6 minutes. Higher recovery was obtained \u0000by extraction with methanol-acetone of 69.51% to 72.59% for protocatechuic acid and \u000069.58% to 126.31% for \u0000the chlorogenic acid. The concentrations of chlorogenic, p-coumaric \u0000and ferulic acids in mangaba extracts were 113.4, 32.1 and 1.5 μg.g-1, respectively while \u0000concentrations of chlorogenic, protocatechuic, gallic, vanillic, ferulic and p-coumaric acids present in umbu fruit were 118.9, 141.3, 3.5, 2.5, \u00002.2 and 1.8 μg.g-1, \u0000respectively.","PeriodicalId":14932,"journal":{"name":"Journal of Analytical Sciences, Methods and Instrumentation","volume":"10 1","pages":"1-10"},"PeriodicalIF":0.0,"publicationDate":"2013-09-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"85300678","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 11

Preparation and Release Properties of Sol-Gel Encapsulated Proteins 溶胶-凝胶包膜蛋白的制备及其释放特性研究

Journal of Analytical Sciences, Methods and Instrumentation Pub Date : 2013-09-23 DOI: 10.4236/JASMI.2013.33A002

Yu‐Cheng Chen, Ching‐Piao Liu, Chun-Kai Yang, Bao-Yu Huang, Chuen‐Ying Liu

{"title":"Preparation and Release Properties of Sol-Gel Encapsulated Proteins","authors":"Yu‐Cheng Chen, Ching‐Piao Liu, Chun-Kai Yang, Bao-Yu Huang, Chuen‐Ying Liu","doi":"10.4236/JASMI.2013.33A002","DOIUrl":"https://doi.org/10.4236/JASMI.2013.33A002","url":null,"abstract":"A glycoprotein, bovine serum albumin \u0000(BSA) was used as a model compound for encapsulation in a sol-gel matrix. Dried \u0000gels were ground into powders and meshed to achieve particle sizes less than \u0000250 μm. The products were washed with phosphate buffer. Capillary \u0000electrophoresis was used to evaluate the encapsulation efficiency and the \u0000kinetic properties of protein release. Several parameters, including the pH and \u0000composition of the background electrolyte, were investigated in an effort to \u0000eliminate the matrix effect from the determination of release kinetics. \u0000Complete separation of the silica matrix from BSA was using phosphate buffer, an \u0000applied voltage of 15 kV, and detection at 278 nm. Kinetic studies indicated \u0000that most of the BSA was released in the first 5 h. The rate of BSA release gradually \u0000decreased, and some BSA after 25 h. These results indicated that dilute potassium phosphate buffer could accelerate \u0000protein release, but this was not observed for the concentrations greater than \u000050 mM. We believe the developed method has potential utility in other systems for in vitro matrix \u0000dissolution and drug release studies.","PeriodicalId":14932,"journal":{"name":"Journal of Analytical Sciences, Methods and Instrumentation","volume":"9 1","pages":"11-16"},"PeriodicalIF":0.0,"publicationDate":"2013-09-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"88458314","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 12

Estimation of Vitamins B-Complex (B 2 , B 3 , B 5 and B 6 ) of Some Leafy Vegetables Indigenous to Bangladesh by HPLC Method 高效液相色谱法测定孟加拉国一些叶菜中维生素B复合物(b2、b3、b5和b6)含量

Journal of Analytical Sciences, Methods and Instrumentation Pub Date : 2013-09-23 DOI: 10.4236/JASMI.2013.33A004

Hasan Md. Nazmul, M. Akhtaruzzaman, S. Zakir

{"title":"Estimation of Vitamins B-Complex (B 2 , B 3 , B 5 and B 6 ) of Some Leafy Vegetables Indigenous to Bangladesh by HPLC Method","authors":"Hasan Md. Nazmul, M. Akhtaruzzaman, S. Zakir","doi":"10.4236/JASMI.2013.33A004","DOIUrl":"https://doi.org/10.4236/JASMI.2013.33A004","url":null,"abstract":"The current experiment was conducted \u0000for the simultaneous determination of several water-soluble vitamins like ribo- flavin (vitamin B2), niacin \u0000(vitamin B3), pantothenic acid (vitamin B5) and \u0000pyridoxine (vitamin B6) in five highly con- sumed local leafy vegetables named as \u0000bottle gourd leaves (Lagenaria vulgaris) (local name Lau shak), green \u0000amaranth leaves (Amaranthus viridis) \u0000(local name Data shak), red amaranth leaves (Amaranthus gangeticuss) \u0000(local name Lal shak), Indian spinach (Basella alba) (local name Pui \u0000shak) and bitter gourd leaves (Momordica charantia) (local name Korola \u0000shak). The analyses were performed by HPLC using an analytical reversed phase \u0000C-18 (ODS column, 250 × 4.6 mm, 5 μm, Phenomenex, Inc.) column with the mobile \u0000phase consisting of a mixture of buffer (hexane sulphonic acid sodium, \u0000potassium dihydrogen phosphate and triethylamine, pH 3.0) and methanol in the \u0000ratio of 96:4 (v/v) at a flow rate of 1 mL/min with UV detection at 210 nm. The \u0000retention times for the vitamins were obtained as 3.61 min, 6.37 min, 9.51 min \u0000and 11.51 min for Vitamins B2, B3, B5 and B6, \u0000respectively. These obtained values of the vitamins were compared with the \u0000values available in published literatures of Deshio Khaddar Pustiman (DKPM), Indian food value (IFV) and United States \u0000Department of Agriculture (USDA).","PeriodicalId":14932,"journal":{"name":"Journal of Analytical Sciences, Methods and Instrumentation","volume":"11 1","pages":"24-29"},"PeriodicalIF":0.0,"publicationDate":"2013-09-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"79562949","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 28

Development and Validation of an Ion Chromatography Method for the Simultaneous Determination of Seven Food Additives in Cheeses 离子色谱法同时测定奶酪中7种食品添加剂的建立与验证

Journal of Analytical Sciences, Methods and Instrumentation Pub Date : 2013-09-23 DOI: 10.4236/JASMI.2013.33A005

M. Iammarino, A. Taranto

{"title":"Development and Validation of an Ion Chromatography Method for the Simultaneous Determination of Seven Food Additives in Cheeses","authors":"M. Iammarino, A. Taranto","doi":"10.4236/JASMI.2013.33A005","DOIUrl":"https://doi.org/10.4236/JASMI.2013.33A005","url":null,"abstract":"Cheeses are characterized by several chemical-physical properties that make it difficult for the microorganisms growth, consequently. The actual European legislation allows the addition of few food additives in this type of food products. In this work, the entire procedure of extraction, purification, chromatographic separations and quali/quantitative determination of seven food additives (sorbic acid, benzoic acid, lactic acid, acetic acid, nitrites, nitrates and phosphates) was developed and applied for the analysis of different types of cheese (mozzarella, cheese spread, semi-hard and hard cheeses). Through validation procedure it was possible to evaluate the most important validation parameters. Extended calibration curves (r > 0.990) were obtained for all the analyzed compounds. Recovery values ranged from 72.8% to 98.4% and a good repeatability was obtained, with precision levels in the range of 0.03% - 0.11% (n = 6). The potential and feasibility of the method were tested by analysing real samples, such as mozzarella, cheese spread, semi-hard and hard cheeses, confirming that the method is well suited to satisfy the demands for accurate confirmation analyses of seven food additives in cheeses, which is especially valuable in official check analyses and in monitoring schemes.","PeriodicalId":14932,"journal":{"name":"Journal of Analytical Sciences, Methods and Instrumentation","volume":"33 1","pages":"30-37"},"PeriodicalIF":0.0,"publicationDate":"2013-09-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"80311025","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 11

Determination of total galactose from dried blood spots—extensive assay evaluation of a CE-marked test-kit 干血斑中总半乳糖的测定——ce标记试剂盒的广泛测定评价

Journal of Analytical Sciences, Methods and Instrumentation Pub Date : 2013-08-23 DOI: 10.4236/JASMI.2013.33020

R. Fingerhut, T. Torresani

{"title":"Determination of total galactose from dried blood spots—extensive assay evaluation of a CE-marked test-kit","authors":"R. Fingerhut, T. Torresani","doi":"10.4236/JASMI.2013.33020","DOIUrl":"https://doi.org/10.4236/JASMI.2013.33020","url":null,"abstract":"Most newborn screening laboratories use CE-marked or FDA-approved test-kits, like in routine clinical chemistry. National regulations require only minimal evaluation from the customer, if the test-kits are used as specified by the manufacturer. The microtiter-based kit-concept is often based on the perception, that the laboratory always processes whole microtiter plates. However, in the daily routine, this is rather a rare exception, which leads to much higher costs per newborn, compared to the costs per assay in the test-kits. In addition the amount of wasted resources is quite high. Performance of the Neonatal Total Galactose kit from Perkin Elmer was tested. We have determined specificity, limit of detection (LOD), limit of quantitation (LOQ), intra and inter assay variation, recovery, stability of measuring signal and reagents. Results were also compared with the Astoria Pacific Spot Check System. In addition, we had (by chance) the opportunity to test 2 kits, which were already expired for more than 3 years. LOD was 165 - 306 μmol/L and LOQ 475 - 703 μmol/L, depending on the definition of LOD/LOQ. Mean recovery was 112.8%, intra assay CVs were 11.3, 7.3, 4.0, and 3.0, and inter assay CVs 28.7, 15.9, 7.8, and 9.3, at 220, 590, 1200, and 2060 μmol/L respectively. Reconstituted and mixed reagents must be used within some hours, and were unstable even if stored at -20℃. However, if the reconstituted galactose substrate reagent and galactose oxidase reagent were only mixed according to the daily requirements, and the rest stored separately at -20℃, they were stable for at least 12 days. The performance of the expired test-kits did not differ from the others. The performance of the Total Galactose kit is comparable to other tests used for newborn screening. However, we could significantly reduce the costs per newborn and reduce unnecessary production of waste, by thorough validation and modification of the assay procedures.","PeriodicalId":14932,"journal":{"name":"Journal of Analytical Sciences, Methods and Instrumentation","volume":"40 1","pages":"163-166"},"PeriodicalIF":0.0,"publicationDate":"2013-08-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"86038547","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 3

Determination of Interaction between NFκB p50 and β-IFN-κB Binding Oligo Using AlphaLISA in HTP Fashion 用AlphaLISA在HTP模式下测定nf -κB p50与β-IFN-κB结合寡核苷酸的相互作用

Journal of Analytical Sciences, Methods and Instrumentation Pub Date : 2013-08-23 DOI: 10.4236/JASMI.2013.33022

Muniasamy Neerathilingam, S. H. A. Gandham, Falguni V Patel, M. Nasiruddin

引用次数: 0

A Design Configuration of an FPGA-Based Coincident Spectrometry System 一种基于fpga的重合光谱系统的设计结构

Journal of Analytical Sciences, Methods and Instrumentation Pub Date : 2013-08-23 DOI: 10.4236/JASMI.2013.33019

P. D. Khang, N. N. Dien, D. Lanh, N. X. Hai, P. N. Tuan, N. Hoa, Nguyen An Son

{"title":"A Design Configuration of an FPGA-Based Coincident Spectrometry System","authors":"P. D. Khang, N. N. Dien, D. Lanh, N. X. Hai, P. N. Tuan, N. Hoa, Nguyen An Son","doi":"10.4236/JASMI.2013.33019","DOIUrl":"https://doi.org/10.4236/JASMI.2013.33019","url":null,"abstract":"In the past, most of popular coincidence spectrometers were normally based on traditional electronics techniques such as time to amplitude conversion or logic selecting coincidence unit. They were complicated and it is not convenient for us to use them. This paper deals with a new design of a contemporary coincidence spectrometer which is based on Field Programmable Gate Arrays (FPGA) devices via Digital Signal Processing (DSP) techniques with Hardware Description Language (VHDL). The outstanding advantage of DSP techniques and FPGA technology is capable of enhancement of the quality of the experimental measurements for nuclear radiation. The designed configuration of the traditional system was tested on the PCI 7811R board of National Instruments while the digital systems were establishing with FPGA devices. The purpose of this work is referring to the principle for construction of an FPGA-based system capable of replacing a conventional system. Therefore, a novel approach for in-house development of digital techniques is presented. The method for designing the system is utilization of slow-fast coincidence configurations with two HPGe detectors obtaining a pair of coincidence events, processing data in DSP algorithms. The significant and noticeable results are the operating frequency of 80 MHz and system timestamp window of approximately 10 ns.","PeriodicalId":14932,"journal":{"name":"Journal of Analytical Sciences, Methods and Instrumentation","volume":"23 1","pages":"158-162"},"PeriodicalIF":0.0,"publicationDate":"2013-08-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"73919124","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

A Study on Measurement of Forest Soil Redox Potential 森林土壤氧化还原电位测定方法的研究

Journal of Analytical Sciences, Methods and Instrumentation Pub Date : 2013-08-23 DOI: 10.4236/JASMI.2013.33017

Mu Liu, Chengde Luo, Xiaoyun Liu, Gang Wan, Bin Dai

引用次数: 4

Quantitation of Genetox Impurities Using a Surrogate Standard Approach 用替代标准法定量Genetox杂质

Journal of Analytical Sciences, Methods and Instrumentation Pub Date : 2013-08-23 DOI: 10.4236/JASMI.2013.33021

Heather Wang, R. Nardi, Y. Bereznitski, R. Helmy, D. Waterhouse

引用次数: 2

The Basis for Design and Manufacture of a DSP-Based Coincidence Spectrometer 基于dsp的符合光谱仪的设计与制造依据

Journal of Analytical Sciences, Methods and Instrumentation Pub Date : 2013-07-01 DOI: 10.4236/JASMI.2013.33018

N. X. Hai, P. N. Tuan, N. N. Dien, D. Lanh, T. Huong, P. N. Son, P. D. Khang

引用次数: 0