Journal of Amino Acids最新文献_第4页

(R)- α -Aminoadipic Acid: A Versatile Precursor for the Synthesis of D-Amino Acids. (R)- α -氨基己二酸:合成d -氨基酸的多功能前体。

Journal of Amino Acids Pub Date : 2013-01-01 Epub Date: 2013-10-10 DOI: 10.1155/2013/252813

Amina Sadiq, Norbert Sewald

引用次数: 2

Potential anticarcinogenic peptides from bovine milk. 牛乳中潜在的抗癌肽。

Journal of Amino Acids Pub Date : 2013-01-01 Epub Date: 2013-02-26 DOI: 10.1155/2013/939804

Giacomo Pepe, Gian Carlo Tenore, Raffaella Mastrocinque, Paola Stusio, Pietro Campiglia

{"title":"Potential anticarcinogenic peptides from bovine milk.","authors":"Giacomo Pepe, Gian Carlo Tenore, Raffaella Mastrocinque, Paola Stusio, Pietro Campiglia","doi":"10.1155/2013/939804","DOIUrl":"https://doi.org/10.1155/2013/939804","url":null,"abstract":"BOVINE MILK POSSESSES A PROTEIN SYSTEM CONSTITUTED BY TWO MAJOR FAMILIES OF PROTEINS: caseins (insoluble) and whey proteins (soluble). Caseins ( α S1, α S2, β , and κ ) are the predominant phosphoproteins in the milk of ruminants, accounting for about 80% of total protein, while the whey proteins, representing approximately 20% of milk protein fraction, include β -lactoglobulin, α -lactalbumin, immunoglobulins, bovine serum albumin, bovine lactoferrin, and lactoperoxidase, together with other minor components. Different bioactivities have been associated with these proteins. In many cases, caseins and whey proteins act as precursors of bioactive peptides that are released, in the body, by enzymatic proteolysis during gastrointestinal digestion or during food processing. The biologically active peptides are of particular interest in food science and nutrition because they have been shown to play physiological roles, including opioid-like features, as well as immunomodulant, antihypertensive, antimicrobial, antiviral, and antioxidant activities. In recent years, research has focused its attention on the ability of these molecules to provide a prevention against the development of cancer. This paper presents an overview of antitumor activity of caseins and whey proteins and derived peptides.","PeriodicalId":14901,"journal":{"name":"Journal of Amino Acids","volume":" ","pages":"939804"},"PeriodicalIF":0.0,"publicationDate":"2013-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1155/2013/939804","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40229759","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 72

Ischemia induces release of endogenous amino acids from the cerebral cortex and cerebellum of developing and adult mice. 缺血诱导发育和成年小鼠大脑皮层和小脑释放内源性氨基酸。

Journal of Amino Acids Pub Date : 2013-01-01 Epub Date: 2013-01-10 DOI: 10.1155/2013/839036

Simo S Oja, Pirjo Saransaari

{"title":"Ischemia induces release of endogenous amino acids from the cerebral cortex and cerebellum of developing and adult mice.","authors":"Simo S Oja, Pirjo Saransaari","doi":"10.1155/2013/839036","DOIUrl":"https://doi.org/10.1155/2013/839036","url":null,"abstract":"Ischemia enhanced release of endogenous neuroactive amino acids from cerebellar and cerebral cortical slices. More glutamate was released in adult than developing mice. Taurine release enhanced by K(+) stimulation and ischemia was more than one magnitude greater than that of GABA or glutamate in the developing cerebral cortex and cerebellum, while in adults the releases were almost comparable. Aspartate release was prominently enhanced by both ischemia and K(+) stimulation in the adult cerebral cortex. In the cerebellum K(+) stimulation and ischemia evoked almost 10-fold greater GABA release in 3-month olds than in 7-day olds. The release of taurine increased severalfold in the cerebellum of 7-day-old mice in high-K(+) media, whereas the K(+)-evoked effect was rather small in adults. In 3-month-old mice no effects of K(+) stimulation or ischemia were seen in the release of aspartate, glycine, glutamine, alanine, serine, or threonine. The releases from the cerebral cortex and cerebellum were markedly different and also differed between developing and adult mice. In developing mice only the release of inhibitory taurine may be large enough to counteract the harmful effects of excitatory amino acids in ischemia in both cerebral cortex and cerebellum, in particular since at that age the release of glutamate and aspartate cannot be described as massive.","PeriodicalId":14901,"journal":{"name":"Journal of Amino Acids","volume":"2013 ","pages":"839036"},"PeriodicalIF":0.0,"publicationDate":"2013-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1155/2013/839036","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"31200863","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 12

An Evaluation of Interindividual Responses to the Orally Administered Neurotransmitter β -Alanine. 对口服神经递质β -丙氨酸的个体间反应的评价。

Journal of Amino Acids Pub Date : 2013-01-01 Epub Date: 2013-06-23 DOI: 10.1155/2013/429847

Sarah Macphee, Ian N Weaver, Donald F Weaver

{"title":"An Evaluation of Interindividual Responses to the Orally Administered Neurotransmitter β -Alanine.","authors":"Sarah Macphee, Ian N Weaver, Donald F Weaver","doi":"10.1155/2013/429847","DOIUrl":"https://doi.org/10.1155/2013/429847","url":null,"abstract":"Previously, we have identified β -alanine as a potential endogenous anticonvulsant molecule. β -Alanine occurs within the human central nervous system and has been identified as both an inhibitory neuromodulator and neurotransmitter that is bioavailable to brain after oral administration. During preliminary compounding trials to ascertain dosing strategies for β -alanine, we noted pronounced differences in the side effect profile experienced by individuals of Asian and Caucasian descent. To investigate whether ethnicity affects β -alanine-induced side effects, we administered 3 g of β -alanine in 200 mL of fruit drink to ten people of each ethnic background and observed them for 30 minutes. Data collected included basic physical statistics (height, age, and weight) and descriptions of all side effects, as reported by participants. We found that participants of Asian descent experienced paraesthesia, but significantly different in time of onset, intensity, and anatomical localization, as compared to the effects experienced by Caucasian participants. Since β -alanine is an endogenous neurotransmitter substance within human brain, these side effect differences were unexpected. ","PeriodicalId":14901,"journal":{"name":"Journal of Amino Acids","volume":"2013 ","pages":"429847"},"PeriodicalIF":0.0,"publicationDate":"2013-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1155/2013/429847","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"31589250","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 15

Cdc48: a swiss army knife of cell biology. Cdc48:细胞生物学的瑞士军刀。

Journal of Amino Acids Pub Date : 2013-01-01 Epub Date: 2013-09-15 DOI: 10.1155/2013/183421

Guem Hee Baek, Haili Cheng, Vitnary Choe, Xin Bao, Jia Shao, Shiwen Luo, Hai Rao

引用次数: 100

The Antitumor Peptide CIGB-552 Increases COMMD1 and Inhibits Growth of Human Lung Cancer Cells. 抗肿瘤肽CIGB-552增加COMMD1并抑制人肺癌细胞生长。

Journal of Amino Acids Pub Date : 2013-01-01 Epub Date: 2013-01-16 DOI: 10.1155/2013/251398

Julio R Fernández Massó, Brizaida Oliva Argüelles, Yelaine Tejeda, Soledad Astrada, Hilda Garay, Osvaldo Reyes, Livan Delgado-Roche, Mariela Bollati-Fogolín, Maribel G Vallespí

{"title":"The Antitumor Peptide CIGB-552 Increases COMMD1 and Inhibits Growth of Human Lung Cancer Cells.","authors":"Julio R Fernández Massó, Brizaida Oliva Argüelles, Yelaine Tejeda, Soledad Astrada, Hilda Garay, Osvaldo Reyes, Livan Delgado-Roche, Mariela Bollati-Fogolín, Maribel G Vallespí","doi":"10.1155/2013/251398","DOIUrl":"https://doi.org/10.1155/2013/251398","url":null,"abstract":"We have demonstrated that the peptide L-2 designed from an alanine scanning of the Limulus-derived LALF32-51 region is a potential candidate for the anticancer therapy and its cell-penetrating capacity is an associated useful property. By the modification in the primary structure of L-2, a second-generation peptide (CIGB-552) was developed. However, the molecular mechanism underlying its cytotoxic activity remains partially unknown. In this study, it was shown that CIGB-552 increases the levels of COMMD1, a protein involved in copper homeostasis, sodium transport, and the NF-κB signaling pathway. We found that CIGB-552 induces ubiquitination of RelA and inhibits the antiapoptotic activity regulated by NF-κB, whereas the knockdown of COMMD1 blocks this effect. We also found that CIGB-552 decreases the antioxidant capacity and induces the peroxidation of proteins and lipids in the tumor cells. Altogether, this study provides new insights into the mechanism of action of the peptide CIGB-552, which could be relevant in the design of future anticancer therapies.","PeriodicalId":14901,"journal":{"name":"Journal of Amino Acids","volume":"2013 ","pages":"251398"},"PeriodicalIF":0.0,"publicationDate":"2013-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1155/2013/251398","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"31231073","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 42

Binding Stoichiometry of a Recombinant Selenophosphate Synthetase with One Synonymic Substitution E197D to a Fluorescent Nucleotide Analog of ATP, TNP-ATP. 具有一个同义取代E197D的重组硒磷酸合成酶与ATP的荧光核苷酸类似物TNP-ATP的结合化学计量学。

Journal of Amino Acids Pub Date : 2013-01-01 Epub Date: 2013-01-30 DOI: 10.1155/2013/983565

Y V Preobrazhenskaya, A I Stenko, M V Shvarts, V Y Lugovtsev

{"title":"Binding Stoichiometry of a Recombinant Selenophosphate Synthetase with One Synonymic Substitution E197D to a Fluorescent Nucleotide Analog of ATP, TNP-ATP.","authors":"Y V Preobrazhenskaya, A I Stenko, M V Shvarts, V Y Lugovtsev","doi":"10.1155/2013/983565","DOIUrl":"https://doi.org/10.1155/2013/983565","url":null,"abstract":"The transformation of the strain DH5α (TM)-T1(R) with plasmid vector pET11a containing the cloned gene of bacterial selenophosphate synthetase (SPS), selD, from the E. coli BL21-Gold (DE3) strain gives an overproducing strain of SPS with one synonymic substitution, E197D. The transformation efficiency was estimated as 8 × 10(8) CFU/ μ g plasmid DNA. 28 mg of highly purified preparation of recombinant SPS capable of binding TNP-ATP was eluted from DEAE-Sephadex column in amount of 15 % from the total soluble protein in crude extract. The fluorescent derivative of ATP, 2'(3')-O-(2,4,6-trinitrophenyl)adenosine-5'-triphosphate (TNP-ATP), was used as a synthetic analog of the substrate for the monitoring and quantitative analysis of the functional activity of SPS. The non-linear regression analysis of the saturation curve of TNP-ATP binding to D197 SPS with GraphPad Prism software fits to a model with 2 distinct binding sites with KDs different in order. The SPS existence in a form of tetramer in given reaction conditions, in accordance with the concentration stoichiometry of 4 moles of TNP-ATP to 1 mole of recombinant protein, is being discussed. The tetramer structure was predicted with molecular modelling software YASARA and modelled in vacuum using steepest descent minimization energy method. We hypothesize here the recombinant SPS exists as a dimer in solution with two active sites capable of ATP binding in each subunit. ","PeriodicalId":14901,"journal":{"name":"Journal of Amino Acids","volume":"2013 ","pages":"983565"},"PeriodicalIF":0.0,"publicationDate":"2013-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1155/2013/983565","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"32251502","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

Acute effects of an energy drink on myocardial function assessed by conventional echo-Doppler analysis and by speckle tracking echocardiography on young healthy subjects. 用常规超声多普勒分析和斑点跟踪超声心动图评估能量饮料对年轻健康受试者心肌功能的急性影响。

Journal of Amino Acids Pub Date : 2013-01-01 Epub Date: 2013-11-10 DOI: 10.1155/2013/646703

Daniele Menci, Francesca Maria Righini, Matteo Cameli, Matteo Lisi, Susanna Benincasa, Marta Focardi, Sergio Mondillo

{"title":"Acute effects of an energy drink on myocardial function assessed by conventional echo-Doppler analysis and by speckle tracking echocardiography on young healthy subjects.","authors":"Daniele Menci, Francesca Maria Righini, Matteo Cameli, Matteo Lisi, Susanna Benincasa, Marta Focardi, Sergio Mondillo","doi":"10.1155/2013/646703","DOIUrl":"https://doi.org/10.1155/2013/646703","url":null,"abstract":"Purpose. Previous studies have underlined the effects of the energy drinks containing caffeine end taurine on the cardiovascular system. The aim of this study was to determine acute changes on echocardiographic parameters assessed by conventional echo-Doppler analysis and by speckle tracking echocardiography after the consumption of an energy drink in a young healthy population. Methods. measurement of blood pressure, electrocardiographic, and echocardiographic examination were performed on 35 healthy subjects (mean age 25 ± 2 years, 16 men), at baseline and one hour after the consumption of a body surface area indexed amount of an energy drink (168 mL/m(2)) containing caffeine (0.03%) and taurine (0.4%). Results. The analysis of left ventricular function showed a significant increase of mean relative values of MAPSE (+11%; P < 0.001), global longitudinal strain (+10%, P = 0.004), and left ventricular twisting (+22%, P < 0.0001) in respect to baseline. Also, right ventricular function parameters appeared significantly increased after energy drink consumption, as TAPSE (+15%, P < 0.0001), global, and free wall right ventricular longitudinal strain (+8%, P = 0.001; +5%, P = 0.1, resp.). Conclusion. In conclusion, the consumption of the ED in our population showed a significant increase of right and left ventricular myocardial function, suggesting a possible positive inotropic effect related to the substances contained therein. ","PeriodicalId":14901,"journal":{"name":"Journal of Amino Acids","volume":"2013 ","pages":"646703"},"PeriodicalIF":0.0,"publicationDate":"2013-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1155/2013/646703","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"31939442","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 25

Amino Acid derivatives as new zinc binding groups for the design of selective matrix metalloproteinase inhibitors. 氨基酸衍生物作为新的锌结合基团用于设计选择性基质金属蛋白酶抑制剂。

Journal of Amino Acids Pub Date : 2013-01-01 Epub Date: 2013-03-10 DOI: 10.1155/2013/178381

Mariateresa Giustiniano, Paolo Tortorella, Mariangela Agamennone, Antonella Di Pizio, Armando Rossello, Elisa Nuti, Isabel Gomez-Monterrey, Ettore Novellino, Pietro Campiglia, Ermelinda Vernieri, Marina Sala, Alessia Bertamino, Alfonso Carotenuto

{"title":"Amino Acid derivatives as new zinc binding groups for the design of selective matrix metalloproteinase inhibitors.","authors":"Mariateresa Giustiniano, Paolo Tortorella, Mariangela Agamennone, Antonella Di Pizio, Armando Rossello, Elisa Nuti, Isabel Gomez-Monterrey, Ettore Novellino, Pietro Campiglia, Ermelinda Vernieri, Marina Sala, Alessia Bertamino, Alfonso Carotenuto","doi":"10.1155/2013/178381","DOIUrl":"https://doi.org/10.1155/2013/178381","url":null,"abstract":"A number of matrix metalloproteinases (MMPs) are important medicinal targets for conditions ranging from rheumatoid arthritis to cardiomyopathy, periodontal disease, liver cirrhosis, multiple sclerosis, and cancer invasion and metastasis, where they showed to have a dual role, inhibiting or promoting important processes involved in the pathology. MMPs contain a zinc (II) ion in the protein active site. Small-molecule inhibitors of these metalloproteins are designed to bind directly to the active site metal ions. In an effort to devise new approaches to selective inhibitors, in this paper, we describe the synthesis and preliminary biological evaluation of amino acid derivatives as new zinc binding groups (ZBGs). The incorporation of selected metal-binding functions in more complex biphenyl sulfonamide moieties allowed the identification of one compound able to interact selectively with different MMP enzymatic isoforms.","PeriodicalId":14901,"journal":{"name":"Journal of Amino Acids","volume":" ","pages":"178381"},"PeriodicalIF":0.0,"publicationDate":"2013-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1155/2013/178381","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40245322","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 24

Ethanol- and/or Taurine-Induced Oxidative Stress in Chick Embryos. 乙醇和/或牛磺酸诱导的鸡胚胎氧化应激。

Journal of Amino Acids Pub Date : 2013-01-01 Epub Date: 2013-03-21 DOI: 10.1155/2013/240537

Emily J Berning, Noah Bernhardson, Kelly Coleman, Dina A Farhat, Courtney M Gushrowski, Alison Lanctot, Benjamin H Maddock, Kathryn G Michels, Luke A Mugge, Catherine M Nass, Sarah M Yearsley, Robert R Miller

{"title":"Ethanol- and/or Taurine-Induced Oxidative Stress in Chick Embryos.","authors":"Emily J Berning, Noah Bernhardson, Kelly Coleman, Dina A Farhat, Courtney M Gushrowski, Alison Lanctot, Benjamin H Maddock, Kathryn G Michels, Luke A Mugge, Catherine M Nass, Sarah M Yearsley, Robert R Miller","doi":"10.1155/2013/240537","DOIUrl":"https://doi.org/10.1155/2013/240537","url":null,"abstract":"Because taurine alleviates ethanol- (EtOH-) induced lipid peroxidation and liver damage in rats, we asked whether exogenous taurine could alleviate EtOH-induced oxidative stress in chick embryos. Exogenous EtOH (1.5 mmol/Kg egg or 3 mmol/Kg egg), taurine (4 μmol/Kg egg), or EtOH and taurine (1.5 mmol EtOH and 4 μmol taurine/Kg egg or 3 mmol EtOH and 4 μmol taurine/Kg egg) were injected into fertile chicken eggs during the first three days of embryonic development (E0-2). At 11 days of development (midembryogenesis), serum taurine levels and brain caspase-3 activities, homocysteine (HoCys) levels, reduced glutathione (GSH) levels, membrane fatty acid composition, and lipid hydroperoxide (LPO) levels were measured. Early embryonic EtOH exposure caused increased brain apoptosis rates (caspase-3 activities); increased brain HoCys levels; increased oxidative-stress, as measured by decreased brain GSH levels; decreased brain long-chain polyunsaturated levels; and increased brain LPO levels. Although taurine is reported to be an antioxidant, exogenous taurine was embryopathic and caused increased apoptosis rates (caspase-3 activities); increased brain HoCys levels; increased oxidative-stress (decreased brain GSH levels); decreased brain long-chain polyunsaturated levels; and increased brain LPO levels. Combined EtOH and taurine treatments also caused increased apoptosis rates and oxidative stress.","PeriodicalId":14901,"journal":{"name":"Journal of Amino Acids","volume":"2013 ","pages":"240537"},"PeriodicalIF":0.0,"publicationDate":"2013-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1155/2013/240537","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"31375304","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 7