Journal Biomedical and Biopharmaceutical Research最新文献_第4页

Cecropia pachystachya protection against preproIAPP cytotoxicity is independent of Ca2+ homeostasis: lessons learned using a novel yeast model of preproIAPP-induced Ca2+ intracellular dysregulation 对preproIAPP细胞毒性的保护是独立于Ca2+稳态的:使用preproIAPP诱导的Ca2+细胞内失调的新酵母模型的经验教训

Journal Biomedical and Biopharmaceutical Research Pub Date : 2021-12-31 DOI: 10.19277/bbr.18.2.272

Sofia Ferreira, R. G. Tavares, N. Saraiva, R. Menezes, A. Raimundo, F. Reginatto

{"title":"Cecropia pachystachya protection against preproIAPP cytotoxicity is independent of Ca2+ homeostasis: lessons learned using a novel yeast model of preproIAPP-induced Ca2+ intracellular dysregulation","authors":"Sofia Ferreira, R. G. Tavares, N. Saraiva, R. Menezes, A. Raimundo, F. Reginatto","doi":"10.19277/bbr.18.2.272","DOIUrl":"https://doi.org/10.19277/bbr.18.2.272","url":null,"abstract":"Despite the progresses in therapeutics, type 2 diabetes is still an epidemic with alarming numbers worldwide. New strategies for prevention and treatments are imperative. A venue of research with potential is the aggregation of Islet Amyloid PolyPeptide (IAPP), a contributor to pancreatic β-cell dysfunction. To address the impact of IAPP on calcium (Ca2+) signalling, we developed a dual reporter yeast model independently expressing preproIAPP-GFP (ppIAPP) and encoding the lacZ gene under the control of Crz1-recognition elements. In this reporter system, ppIAPP induced Crz1 hyperactivation, a yeast transcription factor activated by the Ca2+/calmodulin/calcineurin pathway, which was followed by the increase in β-galactosidase activity. Encouraged by the reported healthy effects of Urticaceae plants against metabolic disturbances, we tested the protective potential of Cecropia pachystachya against IAPP-induced cytotoxicity using the newly designed yeast model. Although C. pachystachya extract exerted no beneficial effects towards the prevention of ppIAPP cytotoxicity, treatment with C. pachystachya enriched C-glycosyl flavonoid fraction (EFF-Cp) significantly improved viability of ppIAPP-expressing cells. Potential bioactivities of C. pachystachya extract and EFF-Cp towards the restoration of Ca2+ homeostasis disrupted by ppIAPP expression were also assessed. Neither prevented Crz1 hyperactivation, suggesting that the EFF-Cp-induced protection against ppIAPP toxicity was mediated by Ca2+-independent mechanisms. Keywords: Amylin, Calcium signaling; Cecropia pachystachya; Diabetes; Islet Amyloid Polypeptide (IAPP)","PeriodicalId":14771,"journal":{"name":"Journal Biomedical and Biopharmaceutical Research","volume":"17 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"90187203","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

An overview of violence and sexual abuse in children: global data, policy responses, and multidisciplinary approaches in health care 儿童暴力和性虐待概述:全球数据、政策应对和卫生保健中的多学科方法

Journal Biomedical and Biopharmaceutical Research Pub Date : 2021-12-26 DOI: 10.19277/bbr.18.2.266

Nuno Coelho, Anabela Neves, João Gregório

{"title":"An overview of violence and sexual abuse in children: global data, policy responses, and multidisciplinary approaches in health care","authors":"Nuno Coelho, Anabela Neves, João Gregório","doi":"10.19277/bbr.18.2.266","DOIUrl":"https://doi.org/10.19277/bbr.18.2.266","url":null,"abstract":"News of sexual abuse against children is increasingly common. This paper aims to describe the historical and social progress of sexual violence against children through a narrative review of the literature, with the goal of understanding how health systems deal with sexual violence against children, as well as to identify the costs to society and to children's personal lives. The history and latest global and national data on sexual abuse against children are presented, revealing that violence against children is emerging as an endemic threat worldwide, resulting in serious social and health problems, with higher prevalence in low- and middle-income countries than in high-income countries. In these higher-resource countries, understanding and detecting what is abuse and what is not abuse in a given situation is supported by a multidisciplinary team, involving professionals from different sectors. Thus, it is important that all actors be enabled to articulate their actions, working with the goal of safeguarding well-being and development of children. Keywords: Child; Sexual abuse; Violence; Health professionals","PeriodicalId":14771,"journal":{"name":"Journal Biomedical and Biopharmaceutical Research","volume":"6 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-12-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"88141482","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Photopletismography in dogs: identification of spectral elements and application in a surgical setting 狗的光谱学:光谱元素的识别及其在外科环境中的应用

Journal Biomedical and Biopharmaceutical Research Pub Date : 2021-12-15 DOI: 10.19277/bbr.18.2.264

Rui Assunção, Clemente Rocha, Luís Lobo, L. Monteiro Rodrigues, J. Requicha

{"title":"Photopletismography in dogs: identification of spectral elements and application in a surgical setting","authors":"Rui Assunção, Clemente Rocha, Luís Lobo, L. Monteiro Rodrigues, J. Requicha","doi":"10.19277/bbr.18.2.264","DOIUrl":"https://doi.org/10.19277/bbr.18.2.264","url":null,"abstract":"This pilot study aimed to assess cardiac and respiratory activity in dogs using photopletismography (PPG) during the presurgical period and to determine the spectral elements composing the measured PPG signal. The studied sample comprised four dogs, two males and two females, ranging from 7 months to 6 years old, from different breeds under anesthetic premedication. PPG signal was obtained at the tail using a PPG sensor connected to a Bitalino hardware board (Plux, Portugal) before and after premedication with 5 µg/kg dexmedetomidine and 0.35 mg/kg methadone by intramuscular injection. The data obtained was analyzed using Matlab (MathWorks, USA) and the PPG signal decomposed into its various frequencies by the Wavelet transform. The estimated minimum and maximum values of heart and respiratory activity were then identified. Seven waves were found. Correspondence with the cardiac and respiratory activities was determined after comparing its frequency with the normal heart and respiratory frequencies in dogs. This study demonstrated that the PPG technique is a valuable diagnostic tool to record both heart and respiratory frequencies in a clinical setting using a wireless system. Keywords: dog, respiratory rate, heart rate, photoplethysmography, anesthesia, Wavelet transform","PeriodicalId":14771,"journal":{"name":"Journal Biomedical and Biopharmaceutical Research","volume":"78 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-12-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"75469720","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Effect of cosmetic films with ascorbic acid and hyaluronic acid on transepidermal water loss 含有抗坏血酸和透明质酸的化妆品薄膜对经皮失水的影响

Journal Biomedical and Biopharmaceutical Research Pub Date : 2021-12-11 DOI: 10.19277/bbr.18.2.262

Isadora Marques Garros Brait, Laura Moretti Aiello, Gislaine Ricci Leonardi, Mariane Massufero Vergilio

{"title":"Effect of cosmetic films with ascorbic acid and hyaluronic acid on transepidermal water loss","authors":"Isadora Marques Garros Brait, Laura Moretti Aiello, Gislaine Ricci Leonardi, Mariane Massufero Vergilio","doi":"10.19277/bbr.18.2.262","DOIUrl":"https://doi.org/10.19277/bbr.18.2.262","url":null,"abstract":"Cosmetic films are structures made of a polymer capable of forming a continuous matrix. Collagen and hyaluronic acid (HA) are components of the skin extracellular matrix and act to maintain its elasticity and resistance. Ascorbic acid (AA) acts in the formation of collagen fibers, contributing to the maintenance of their firmness and elasticity. This study aimed to evaluate the moisturizing properties of cosmetic films with AA or HA in their composition. It was also investigated whether the presence of caprylyl glycol would interfere with the TWEL of the formulations. The occlusive properties of the films were assessed based on the reduction of transepidermal water loss (TEWL) in 20 healthy participants using a Tewameter probe. At T30, 30 minutes after application, all formulations showed a reduction in TEWL compared to the control. In general, the formulations that included caprylyl glycol presented the best TEWL results after 30 min of application. However, after 60 minutes, the transepidermal water loss began to return to its basal levels. This study showed a significant difference in the TEWL value, suggesting that the topical application of the films can contribute to the improvement and maintenance of skin hydration. Keywords: cosmetic films; collagen; hyaluronic acid; ascorbic acid; skin barrier","PeriodicalId":14771,"journal":{"name":"Journal Biomedical and Biopharmaceutical Research","volume":"112 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-12-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"75722581","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Probiotics in the gut-skin axis – the case of kefir 肠道-皮肤轴中的益生菌——以开菲尔为例

Journal Biomedical and Biopharmaceutical Research Pub Date : 2021-11-04 DOI: 10.19277/bbr.18.2.261

Emília Alves, P. Rijo, L. Monteiro Rodrigues, C. Rosado

{"title":"Probiotics in the gut-skin axis – the case of kefir","authors":"Emília Alves, P. Rijo, L. Monteiro Rodrigues, C. Rosado","doi":"10.19277/bbr.18.2.261","DOIUrl":"https://doi.org/10.19277/bbr.18.2.261","url":null,"abstract":"The intestinal microbiota is linked to important functions in the host. Alterations in its composition and/or its by-products, causing loss of homeostasis, contribute to dysfunctions in other organic systems, including the skin, hence suggesting a gut-skin relationship. The oral administration of probiotics, widely associated with improved intestinal health, can act through an immunomodulatory response, both locally and systemically, presenting itself as potentially beneficial in inflammatory skin diseases such as atopic dermatitis. Traditional kefir, consumed for centuries as a health-promoting natural food, has its biological activity attributed both to the presence of a complex microbiota and to the action of the metabolites released during fermentation. The biological activity of kefir has been demonstrated in part by its ability to positively impact the intestinal microbiota, mainly based on animal models and in vitro, thus providing limited information. The nutritional and microbiological value of kefir makes its application as a probiotic in the gut-skin relationship a topic of of significant interest. This review aimed to explore the impact of probiotics as regulators of the gut-skin axis, focusing on the current knowledge of kefir as a health-promoting food. Keywords: kefir, probiotic, gut-skin axis, skin health, atopic dermatitis","PeriodicalId":14771,"journal":{"name":"Journal Biomedical and Biopharmaceutical Research","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-11-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"91089273","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

This issue in brief - a renewed vision 简而言之，这个问题是一个新的愿景

Journal Biomedical and Biopharmaceutical Research Pub Date : 2021-07-06 DOI: 10.19277/bbr.18.1.e

L. Monteiro Rodrigues

引用次数: 0

Use of chromium-enriched yeast in the manufacture of French bread 在制造法国面包中使用富铬酵母

Journal Biomedical and Biopharmaceutical Research Pub Date : 2021-06-01 DOI: 10.19277/BBR.18.1.255

Carla Sá, A. Barata, Carla Ramalho, M. Nunes, Nelson Tavares

引用次数: 0

General Toxicity screening of Royleanone derivatives using an Artemia salina model 利用盐蒿模型筛选Royleanone衍生物的一般毒性

Journal Biomedical and Biopharmaceutical Research Pub Date : 2021-06-01 DOI: 10.19277/bbr.18.1.254

E. Ntungwe, Vera M. S. Isca, A. Díaz-Lanza, Carlos A. M. Afonso, P. Rijo

{"title":"General Toxicity screening of Royleanone derivatives using an Artemia salina model","authors":"E. Ntungwe, Vera M. S. Isca, A. Díaz-Lanza, Carlos A. M. Afonso, P. Rijo","doi":"10.19277/bbr.18.1.254","DOIUrl":"https://doi.org/10.19277/bbr.18.1.254","url":null,"abstract":"Cancer is the second leading cause of death globally. World Health Organization recorded an estimated 9.6 million deaths attributed to cancer in 2018. There is an urgent need for new anticancer drugs with novel modes of action. Natural products remain a valuable source for the identification and development of novel treatment options for cancer. Plectranthus species are well-known medicinal species used extensively for the treatment of different illnesses. These species are rich in diterpenoids which are reported to be responsible for various pharmacological activities including cytotoxic activities. Brine shrimp (Artemia salina) is broadly used in lethality studies and this model is a convenient starting point for cytotoxicity study when screening for general toxicity of natural products. This assay is based on their ability to kill a laboratory cultured nauplii. In this work, we report the general toxicity of some derivatives from 6,7-dehydroroyleanone (1) and 7α-acetoxy-6β-hydroxyroyleanone (2) compounds. The A. salina bioassay is a simple, rapid, and low-cost test, and is very useful for preliminary assessment of general toxicity in natural products thereby guiding the determination of possible biological activities.","PeriodicalId":14771,"journal":{"name":"Journal Biomedical and Biopharmaceutical Research","volume":"81 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"83656402","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Acceptability of kefir produced by fermentation of Portuguese milk with CIDCA AGK1 grains in a sample of Portuguese consumers 葡萄牙牛奶与CIDCA AGK1谷物发酵生产的开菲尔酒在葡萄牙消费者样本中的可接受性

Journal Biomedical and Biopharmaceutical Research Pub Date : 2021-04-01 DOI: 10.19277/bbr.18.1.252

Emília Alves, P. Rijo, L. Monteiro Rodrigues, C. Rosado

{"title":"Acceptability of kefir produced by fermentation of Portuguese milk with CIDCA AGK1 grains in a sample of Portuguese consumers","authors":"Emília Alves, P. Rijo, L. Monteiro Rodrigues, C. Rosado","doi":"10.19277/bbr.18.1.252","DOIUrl":"https://doi.org/10.19277/bbr.18.1.252","url":null,"abstract":"Fermented foods, such as kefir, tend to be characterized by their unique flavor and aroma. Sensory perception of this type of food or beverage is a key factor for the general consumer acceptance of the product, which can be assessed through sensory tests. Conventional sensory tests include acceptance tests where consumers, not trained panelists, are asked to express their degree of like on a hedonic scale, where the level of acceptability of foods does not require a choice between alternatives. The most commonly used scale for testing consumer acceptability of foods is the 9-point hedonic scale. An average score of 7 or higher on the acceptability test indicates a high sensory quality and represents a good acceptance of the product by the panel. This study aimed to evaluate the acceptance of a kefir drink in a sample of Portuguese consumers. The acceptability test of the kefir obtained by fermentation of Portuguese milk with CIDCA AGK1 kefir grains was conducted in a group of 19 consumers using a 9-point hedonic scale and produced a mean score of 7.00 ± 1.15, which correlates with a qualitative rating of “Like moderately.” This work is part of an ongoing study, designated DermapBio, conducted by our research center, with an aim to evaluate the benefits of kefir ingestion for cutaneous health.","PeriodicalId":14771,"journal":{"name":"Journal Biomedical and Biopharmaceutical Research","volume":"21 1","pages":"1-9"},"PeriodicalIF":0.0,"publicationDate":"2021-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"83372917","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

Systemic treatment of HER2-positive breast cancer patients with brain metastases: current status and exploratory case study in a Portuguese cohort her2阳性乳腺癌脑转移患者的全身治疗:葡萄牙队列的现状和探索性病例研究

Journal Biomedical and Biopharmaceutical Research Pub Date : 2021-04-01 DOI: 10.19277/BBR.18.1.253

P. Luz, E. Campoa, R. Gameiro, Marta Vaz, I. Fernandes, J. Magalhães, B. Gosálbez, S. Braga, J. Costa, A. Fernandes

{"title":"Systemic treatment of HER2-positive breast cancer patients with brain metastases: current status and exploratory case study in a Portuguese cohort","authors":"P. Luz, E. Campoa, R. Gameiro, Marta Vaz, I. Fernandes, J. Magalhães, B. Gosálbez, S. Braga, J. Costa, A. Fernandes","doi":"10.19277/BBR.18.1.253","DOIUrl":"https://doi.org/10.19277/BBR.18.1.253","url":null,"abstract":"Over the last years, the incidence of brain metastases in HER2 breast cancer patients has increased. Surgery and radiotherapy are the current standard local therapies. Nevertheless, it is unclear which and when systemic treatment should be applied in addition to local treatment. This work aims to present an updated review of current systemic treatment options for patients with HER2+ metastatic breast cancer with brain metastases and to present a case study of clinical cases that occurred in a Portuguese population. The methodology of this work included a literature search in PubMed for the impact of HER2-targeting agents, such as pertuzumab, trastuzumab emtansine (T-DM1), lapatinib, neratinib, trastuzumab deruxtecan, and tucatinib in the treatment of patients with HER2+ breast cancer with brain metastases. Then, a cohort of Portuguese patients with HER2+ breast cancer (n=44) was analyzed. In this exploratory study, considering a follow-up of 23.9 months, three patients (6.8%) developed brain metastases despite having shown a complete pathological response. The role of systemic treatment for patients with HER2 breast cancer with brain metastases has rapidly evolved following recent successes in phase II and III clinical trials. The biggest challenge is how to integrate systemic and local treatment in the management of these patients.","PeriodicalId":14771,"journal":{"name":"Journal Biomedical and Biopharmaceutical Research","volume":"98 1","pages":"1-14"},"PeriodicalIF":0.0,"publicationDate":"2021-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"76855932","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0