ISRN Hematology最新文献

Preventative effects of caffeic Acid phenyl ester on cadmium intoxication induced hematological and blood coagulation disturbances and hepatorenal damage in rats. 咖啡酸苯酯对镉中毒大鼠血液学、凝血功能紊乱及肝肾损害的预防作用。

ISRN Hematology Pub Date : 2014-03-30 eCollection Date: 2014-01-01 DOI: 10.1155/2014/764754

Tariq Helal Ashour

{"title":"Preventative effects of caffeic Acid phenyl ester on cadmium intoxication induced hematological and blood coagulation disturbances and hepatorenal damage in rats.","authors":"Tariq Helal Ashour","doi":"10.1155/2014/764754","DOIUrl":"https://doi.org/10.1155/2014/764754","url":null,"abstract":"The preventative effect of caffeic acid phenyl ester (CAPE) against hematological, blood coagulation, and hepatorenal disturbances in cadmium (Cd) intoxication was investigated in rats. Male Wistar rats were randomly assigned into control group, Cd-group, and Cd + CAPE group. Cd intoxication was induced by intraperitoneal injection (i.p.) of CdCl2 (1 mg/kg/day) for 21 days, and CAPE was daily given (10 micromol/kg; i.p.) for also 21 days. The results showed that Cd intoxication impaired hepatorenal function and significantly prolonged prothrombin time and activated partial thromboplastin time and decreased fibrinogen level, red blood cells and platelets counts, hemoglobin concentration, hematocrit, mean corpuscular volume, mean corpuscular hemoglobin, and mean corpuscular hemoglobin concentration. Interestingly, all these hematological, blood coagulation, and hepatorenal deteriorations of Cd toxicity were significantly prevented by CAPE. Additionally, CAPE significantly reversed the significant decreases in levels of total reduced glutathione and superoxide dismutase and increases in levels of thiobarbituric acid reactive substances that were observed in the sera and liver and kidney homogenates of Cd group. It is concluded that CAPE is a promising compound that can counteract the hematological and blood coagulation disturbances, oxidative stress, and hepatorenal damages in Cd intoxication. However, further studies are crucially needed to improve this treatment in patients. ","PeriodicalId":14727,"journal":{"name":"ISRN Hematology","volume":"2014 ","pages":"764754"},"PeriodicalIF":0.0,"publicationDate":"2014-03-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1155/2014/764754","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"32489676","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 33

Relevant aspects of centrifugation step in the preparation of platelet-rich plasma. 富血小板血浆制备中离心步骤的相关方面。

ISRN Hematology Pub Date : 2014-03-25 eCollection Date: 2014-01-01 DOI: 10.1155/2014/176060

Amanda G M Perez, José Fábio S D Lana, Ana Amélia Rodrigues, Angela Cristina M Luzo, William D Belangero, Maria Helena A Santana

{"title":"Relevant aspects of centrifugation step in the preparation of platelet-rich plasma.","authors":"Amanda G M Perez, José Fábio S D Lana, Ana Amélia Rodrigues, Angela Cristina M Luzo, William D Belangero, Maria Helena A Santana","doi":"10.1155/2014/176060","DOIUrl":"https://doi.org/10.1155/2014/176060","url":null,"abstract":"Introduction. Platelet-Rich Plasma (PRP) is rich in growth factors, playing important role in tissue healing. The wide variation of reported protocols for preparation of PRP leads to variable compositions, which induce different biological responses and prevent results comparison. This study aims to highlight relevant aspects of the centrifugation step to obtain reproducible results and overall quality. Material and Methods. Samples of blood were collected from 20 healthy donors that have signed free informed consent. Two centrifugation steps (spins) were analyzed for the influence of centrifugal acceleration, time, processed volume, and platelet gradient. The Pure Platelet-Rich Plasma (P-PRP) was characterized as platelet concentration, integrity, and viability (sP-selectin measurement). Results. Lower centrifugal accelerations favour platelet separation. The processing of 3.5 mL of blood at 100 ×g for 10 min (1st spin), 400 ×g for 10 min (2nd spin), withdrawing 2/3 of remnant plasma, promoted high platelet recovery (70-80%) and concentration (5x) maintaining platelet integrity and viability. The recovery of platelets was reduced for a larger WB volume (8.5 mL) processed. Conclusion. Centrifugal acceleration, time, WB processed volume, and minimization of the platelet gradient before sampling are relevant aspects to ensure reproducible compositions within the autologous nature of PRP. ","PeriodicalId":14727,"journal":{"name":"ISRN Hematology","volume":"2014 ","pages":"176060"},"PeriodicalIF":0.0,"publicationDate":"2014-03-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1155/2014/176060","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"32489673","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 128

Phagocytized neutrophil fragments in the bone marrow: a phenomenon most commonly associated with hodgkin lymphoma. 骨髓中被吞噬的中性粒细胞碎片:一种与霍奇金淋巴瘤最常见的现象。

ISRN Hematology Pub Date : 2014-03-18 eCollection Date: 2014-01-01 DOI: 10.1155/2014/363854

Michael A Arnold, Samir B Kahwash

{"title":"Phagocytized neutrophil fragments in the bone marrow: a phenomenon most commonly associated with hodgkin lymphoma.","authors":"Michael A Arnold, Samir B Kahwash","doi":"10.1155/2014/363854","DOIUrl":"https://doi.org/10.1155/2014/363854","url":null,"abstract":"Bone marrow macrophages containing other cells, or large pieces of other cells, represent a distinctive feature of diseases such as Hemophagocytic Lymphohistiocytosis (HLH) and Rosai-Dorfman disease. We describe a distinct variation of phagocytic histiocyte morphology, featuring histiocytes containing predominantly fragments of neutrophil nuclei. We retrospectively reviewed initial bone marrow samples for Hodgkin lymphoma, Burkitt lymphoma, Ewing sarcoma, or evaluation for nonneoplastic conditions, scoring the presence or absence of the above-described histiocytes. We find that these histiocytes, which we term \"fragmentophages,\" are associated with staging marrow sampling for malignancy, especially Hodgkin lymphoma (Hodgkin lymphoma: 28/34 or 82.4%, Ewing sarcoma: 11/26 or 42.3%, Burkitt lymphoma: 4/13 or 30.8%). These cells are significantly less common in marrow samples for nonneoplastic conditions (4/21 or 19.0%). Fragmentophages are significantly associated with malignancy, especially Hodgkin lymphoma, and their recognition has the potential to provide a clue to an underlying malignancy. ","PeriodicalId":14727,"journal":{"name":"ISRN Hematology","volume":"2014 ","pages":"363854"},"PeriodicalIF":0.0,"publicationDate":"2014-03-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1155/2014/363854","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"32510260","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 5

Coexisting iron deficiency anemia and Beta thalassemia trait: effect of iron therapy on red cell parameters and hemoglobin subtypes. 缺铁性贫血和地中海贫血共存的特点:铁治疗对红细胞参数和血红蛋白亚型的影响

ISRN Hematology Pub Date : 2014-03-12 eCollection Date: 2014-01-01 DOI: 10.1155/2014/293216

Sarika Verma, Ruchika Gupta, Madhur Kudesia, Alka Mathur, Gopal Krishan, Sompal Singh

{"title":"Coexisting iron deficiency anemia and Beta thalassemia trait: effect of iron therapy on red cell parameters and hemoglobin subtypes.","authors":"Sarika Verma, Ruchika Gupta, Madhur Kudesia, Alka Mathur, Gopal Krishan, Sompal Singh","doi":"10.1155/2014/293216","DOIUrl":"https://doi.org/10.1155/2014/293216","url":null,"abstract":"Background. Coexistence of iron deficiency anemia (IDA) and beta thalassemia trait (BTT) has been the topic of few studies. However, no study from our country was found evaluating the effect of iron therapy in patients with concomitant IDA and BTT. Methods. Over a period of two years, 30 patients with concomitant IDA and BTT were included. All the patients had a complete blood count, serum iron studies, and thalassemia screening using BIORADTM hemoglobin testing system. The patients received oral iron therapy in appropriate dosages for a period of twenty weeks, after which all the investigations were repeated. Appropriate statistical methods were applied for comparison of pre- and posttherapy data. Results. All except two patients were adults with a marked female preponderance. Oral iron therapy led to statistically significant improvement in hemoglobin, red cell indices (P < 0.05), and marked change in serum iron, ferritin, and HbA2 levels (P < 0.001). There was a significant reduction in the total iron binding capacity levels. Conclusion. The present study shows the frequent occurrence of iron deficiency anemia in patients with beta thalassemia trait, which can potentially confound the diagnosis of the latter. Hence, iron deficiency should be identified and rectified in patients with suspicion of beta thalassemia trait. ","PeriodicalId":14727,"journal":{"name":"ISRN Hematology","volume":"2014 ","pages":"293216"},"PeriodicalIF":0.0,"publicationDate":"2014-03-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1155/2014/293216","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"32489674","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 39

Prevalence of deletional alpha thalassemia and sickle gene in a tribal dominated malaria endemic area of eastern India. 印度东部部落疟疾流行区α型地中海贫血缺失和镰状基因的流行。

ISRN Hematology Pub Date : 2014-03-11 eCollection Date: 2014-01-01 DOI: 10.1155/2014/745245

Prasanta Purohit, Snehadhini Dehury, Siris Patel, Dilip Kumar Patel

{"title":"Prevalence of deletional alpha thalassemia and sickle gene in a tribal dominated malaria endemic area of eastern India.","authors":"Prasanta Purohit, Snehadhini Dehury, Siris Patel, Dilip Kumar Patel","doi":"10.1155/2014/745245","DOIUrl":"https://doi.org/10.1155/2014/745245","url":null,"abstract":"Inherited hemoglobin disorders like alpha thalassemia and sickle gene are common in the Indian subcontinent. These disorders in the heterozygous state act as malaria resistance genes and influence the susceptibility to Plasmodium falciparum malaria. There is inadequate knowledge about the epidemiology of these malaria resistance genes in the tribal dominated malaria endemic region of the state of Odisha in eastern India. A cross sectional prevalence study was undertaken in 594 subjects in five tribal populations in this region, namely, Sahara (42.4%), Kutia Kandha (30.0%), Kuda (15.8%), Gond (9.8%), and Oraon (2.0%). Sickling test, Hb electrophoresis, HPLC, and molecular studies were undertaken to diagnose the prevalence of sickle allele, β -thalassemia allele, and deletional alpha thalassemia. Sickle and β thalassemia alleles were found in 13.1% and 3.4% of subjects, respectively. Sickle allele was found both in heterozygous (10.1%) and homozygous state (3.03%). The prevalence of alpha thalassemia was 50.84% with an allelic frequency of 0.37. Both α (-3.7) and α (-4.2) alpha thalassemia were detected with an allele frequency of 0.33 and 0.04, respectively. The high prevalence of alpha thalassemia and sickle gene in this population is probably due to selection pressure of endemic malaria in this part of India. ","PeriodicalId":14727,"journal":{"name":"ISRN Hematology","volume":"2014 ","pages":"745245"},"PeriodicalIF":0.0,"publicationDate":"2014-03-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1155/2014/745245","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"32324465","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 32

Pretransplant comorbidities maintain their impact on allogeneic stem cell transplantation outcome 5 years posttransplant: a retrospective study in a single german institution. 移植前合并症对移植后5年同种异体干细胞移植结果的影响:一项来自德国一家机构的回顾性研究。

ISRN Hematology Pub Date : 2014-03-05 eCollection Date: 2014-01-01 DOI: 10.1155/2014/853435

Jens M Chemnitz, Geothy Chakupurakal, Maya Bäßler, Udo Holtick, Sebastian Theurich, Alexander Shimabukuro-Vornhagen, Silke Leitzke, Michael S Von Bergwelt-Baildon, Christof Scheid

{"title":"Pretransplant comorbidities maintain their impact on allogeneic stem cell transplantation outcome 5 years posttransplant: a retrospective study in a single german institution.","authors":"Jens M Chemnitz, Geothy Chakupurakal, Maya Bäßler, Udo Holtick, Sebastian Theurich, Alexander Shimabukuro-Vornhagen, Silke Leitzke, Michael S Von Bergwelt-Baildon, Christof Scheid","doi":"10.1155/2014/853435","DOIUrl":"https://doi.org/10.1155/2014/853435","url":null,"abstract":"The introduction of reduced-intensity conditioning regimens has allowed elderly patients with preexisting comorbidities access to the potentially curative allogeneic stem cell transplantation. Patient's comorbidities at the time of treatment consideration play a significant role in transplant outcome in terms of both overall survival (OS) and nonrelapse mortality (NRM). The hematopoietic stem cell transplantation comorbidity index (HCT-CI) quantifies these patient specific risks and has established itself as a major tool in the pretransplant assessment of patients. Many single center and multicenter studies have assessed the HCT-CI score and reported conflicting outcomes. The present study aimed to evaluate the HCT-CI in a single large European transplant centre. 245 patients were retrospectively analyzed and the predictive value of the score was assessed with respect to OS and NRM. We confirm that the HCT-CI predicts outcome for both OS and NRM. Moreover, we identified age of the patient as an independent prognostic parameter for OS. Incorporation of age in the HCT-CI would improve its ability to prognosticate and allow the transplant physician to assess the patient specific risks appropriately at the time of counseling for transplant. ","PeriodicalId":14727,"journal":{"name":"ISRN Hematology","volume":"2014 ","pages":"853435"},"PeriodicalIF":0.0,"publicationDate":"2014-03-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1155/2014/853435","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"32261096","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 7

Oral and dental considerations in pediatric leukemic patient. 儿童白血病患者的口腔和牙科注意事项。

ISRN Hematology Pub Date : 2014-03-04 eCollection Date: 2014-01-01 DOI: 10.1155/2014/895721

Chiyadu Padmini, K Yellamma Bai

引用次数: 0

The problem of anaemia in patients with colorectal cancer. 结直肠癌患者的贫血问题。

ISRN Hematology Pub Date : 2014-02-12 eCollection Date: 2014-01-01 DOI: 10.1155/2014/547914

M Khanbhai, M Shah, G Cantanhede, S Ilyas, T Richards

{"title":"The problem of anaemia in patients with colorectal cancer.","authors":"M Khanbhai, M Shah, G Cantanhede, S Ilyas, T Richards","doi":"10.1155/2014/547914","DOIUrl":"https://doi.org/10.1155/2014/547914","url":null,"abstract":"Background. Surgical patients are often anaemic preoperatively subsequently requiring blood transfusion. The aim of this study was to assess the problem of anaemia and transfusion rates in patients undergoing surgery for colorectal cancer. Methods. Haemoglobin levels and transfusion requirements were assessed retrospectively in 199 sequential patients operated on for colorectal cancer. This was followed by prospective analysis of 147 patients to correlate preoperative anaemia, stage of bowel cancer, and operation performed with rates of blood transfusion and length of hospital stay. Results. Preoperatively 44% patients were anaemic retrospectively and 60% prospectively. Anaemia increased the risk of transfusion in both studies (69% anaemic versus 31% nonanaemic, P = 0.002 in retrospective series, and 83.7% versus 16.3%, P < 0.0001 in prospective series). Anaemia was proportionally higher in patients with Dukes B (65.2%) and Dukes C (66.6%) than in patients with Dukes A (28.5%). Length of stay was prolonged in transfused patients excluding those requiring major blood transfusion (median 13 versus 7 days, P < 0.0001). Transfusion was also associated with higher mortality (P = 0.05). Conclusion. Anaemia is common in patients with colorectal cancer. Anaemic patients were at high risk of receiving blood transfusion, which in turn increased length of stay and mortality. ","PeriodicalId":14727,"journal":{"name":"ISRN Hematology","volume":"2014 ","pages":"547914"},"PeriodicalIF":0.0,"publicationDate":"2014-02-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1155/2014/547914","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"32232655","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 19

Evaluation of the utility of thromboelastography in a tertiary trauma care centre. 评价血栓弹性成像在三级创伤护理中心的效用。

ISRN Hematology Pub Date : 2014-02-12 eCollection Date: 2014-01-01 DOI: 10.1155/2014/849626

Arulselvi Subramanian, Venencia Albert, Deepak Agrawal, Renu Saxena, Ravindra Mohan Pandey

{"title":"Evaluation of the utility of thromboelastography in a tertiary trauma care centre.","authors":"Arulselvi Subramanian, Venencia Albert, Deepak Agrawal, Renu Saxena, Ravindra Mohan Pandey","doi":"10.1155/2014/849626","DOIUrl":"https://doi.org/10.1155/2014/849626","url":null,"abstract":"Background. Thromboelastography (TEG) unlike conventional coagulation assays evaluates the dynamic interaction of clotting factors and platelets indicating an overall clot quality. Literature assessing the efficacy of TEG in identifying trauma associated bleeding is lacking. Aim. To compare TEG with conventional plasma based coagulation tests and assess whether TEG can serve as a screening test or replace the conventional routine test. Materials. Retrospective data was collected for 150 severe trauma patients. Patients with known evidence of severe comorbidities, which may influence the outcome, were excluded. Detailed evaluation of the patient's clinical and laboratory records was conducted. Diagnostic characteristics such as sensitivity, specificity, and accuracy were calculated. Results. Fifty-one patients were defined as coagulopathic by the conventional coagulation test, 30 by the laboratory established range for TEG indices and 105 by manufactures range. Specificity and sensitivity for the laboratory established range for TEG were 29.4% and 84.8%; for manufactures range sensitivity was 74.5%, specificity was 32.3%. Conclusion. We observed that conventional coagulation assays are the most sensitive tests for diagnosis of coagulopathy due to trauma. However in emergency trauma situations, where immediate corrective measures need to be taken, coagulation parameters and conventional coagulation tests may cause delay. TEG can give highly specific results depicting the underlying coagulopathy. ","PeriodicalId":14727,"journal":{"name":"ISRN Hematology","volume":"2014 ","pages":"849626"},"PeriodicalIF":0.0,"publicationDate":"2014-02-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1155/2014/849626","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"32232269","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 13

Association of body mass index with hemoglobin concentration and iron parameters in Iranian population. 伊朗人群身体质量指数与血红蛋白浓度和铁参数的关系

ISRN Hematology Pub Date : 2014-02-10 eCollection Date: 2014-01-01 DOI: 10.1155/2014/525312

Akram Ghadiri-Anari, Narjes Nazemian, Hassan-Ali Vahedian-Ardakani

引用次数: 51