Japanese Journal of Forensic Science and Technology最新文献_第6页

Hair testing for hypnotics in drug-facilitated sexual assault 在毒品性侵案中对催眠者进行头发检测

Japanese Journal of Forensic Science and Technology Pub Date : 2021-01-01 DOI: 10.3408/jafst.r022

N. Shima, M. Katagi

引用次数: 0

Development and demonstration of cannabis DNA detection kit using DNA chromatography chip DNA色谱芯片大麻DNA检测试剂盒的研制与演示

Japanese Journal of Forensic Science and Technology Pub Date : 2021-01-01 DOI: 10.3408/jafst.786

Tadashi Yamamuro, M. Shigehiko, Naoki Tachiiri, Ayumu Ishii, S. Matsuta, Y. Iwata, Hiroki Segawa, K. Kuwayama, K. Tsujikawa, T. Kanamori, H. Inoue

引用次数: 1

Geographic distribution of Y-STR haplotypes and Y-haplogroups among Miyazaki Prefecture residents 宫崎县居民Y-STR单倍型和y -单倍群的地理分布

Japanese Journal of Forensic Science and Technology Pub Date : 2021-01-01 DOI: 10.3408/jafst.778

Hirofumi Nohara, Ikuko Maeda, Rinnosuke Hisazumi, T. Uchiyama, H. Hirashima, Masahito Nakata, R. Ohno, Tetsuro Hasegawa, K. Shimizu

引用次数: 0

Non-destructive discrimination of white papers by the most frequent brightness value of images 利用图像最常出现的亮度值对白纸进行无损判别

Japanese Journal of Forensic Science and Technology Pub Date : 2021-01-01 DOI: 10.3408/JAFST.799

Keiko Matsunaga, M. Shimoyama

{"title":"Non-destructive discrimination of white papers by the most frequent brightness value of images","authors":"Keiko Matsunaga, M. Shimoyama","doi":"10.3408/JAFST.799","DOIUrl":"https://doi.org/10.3408/JAFST.799","url":null,"abstract":"Non-destructive discrimination of white paper sheets of 9 diŠerent notepads was tried by analyzing the color of the images. All of the paper sheets of these notepads were scanned to obtain re‰ection, transmission, and ‰uorescence images using the scanner with multiple light sources. The scanning condition was 300 ppi and 24 bit RGB color, namely 256 gradations. We examined the brightness values of the R, G, and B channels in the width of 640 pixels and the height of 1020 pixels, a card size, from each of the obtained three images. Since the brightness values were uneven within one paper, the most frequent values of those were used for comparison of the notepads. The most frequent values were nine kinds for one paper because each of the obtained three images had three channels. The most frequent values had values with a range in one notepad. There-fore, those ranges were nine kinds for one notepad. As a result, comparing nine ranges of the most frequent values of one notepad with other notepads, 9 diŠerent notepads could be discriminated from each other by some of nine ranges. study indicates that the image analysis using the most frequent values of the brightness values is useful for non-destructive discrimination of white paper sheets in forensic science.","PeriodicalId":14709,"journal":{"name":"Japanese Journal of Forensic Science and Technology","volume":"3 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"72867223","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Application of probe electrospray ionization mass spectrometry to the analysis of poisons and drugs in adulterated foods and beverages 探针电喷雾电离质谱法在掺假食品和饮料中毒物和药物分析中的应用

Japanese Journal of Forensic Science and Technology Pub Date : 2021-01-01 DOI: 10.3408/jafst.782

Misato Wada, H. Kamata, N. Shima, Akari Ishikawa, Ryutaro Asai, Atsushi Nitta, Hidenao Kakehashi, Shihoko Nakano-Fujii, S. Matsuta, K. Sasaki, Tooru Kamata, H. Nishioka, A. Miki, Tasuku Murata, K. Ogata, H. Tsuchihashi, M. Katagi

引用次数: 0

Petroleum-based liquid fuel soaking and burning properties on various floors, detectable period of detector tube for liquid fuel from fire debris 石油基液体燃料在各种地板上的浸泡和燃烧特性，探测管对火灾碎片中液体燃料的探测周期

Japanese Journal of Forensic Science and Technology Pub Date : 2021-01-01 DOI: 10.3408/jafst.781

Takao Hagiwara, I. Matsuoka, M. Nakagawa, Toshikazu Ichikawa, Katsuhiro Okamoto, M. Honma, M. Kasamatsu, Yasuhiro Suzuki

引用次数: 0

Forensic identification of internal transcribed spacer region-targeting plant and fungal species by direct sequencing 内部转录间隔区靶向植物和真菌物种的直接测序法医鉴定

Japanese Journal of Forensic Science and Technology Pub Date : 2021-01-01 DOI: 10.3408/jafst.814

Yukinobu Kutsuwada

引用次数: 0

Evaluation of the MX908 portable mass spectrometer for the detection of chemical warfare agents MX908便携式质谱仪用于化学战剂检测的评估

Japanese Journal of Forensic Science and Technology Pub Date : 2021-01-01 DOI: 10.3408/jafst.816

K. Yano, Shota Sumi, Takuya Nishisako, H. Miyaguchi, Akinori Yamaguchi, Mai Otsuka, Yuko Kazui, T. Ohmori, Tokita Kenichi, Soichiro Yamamoto, Higashiyama Hisamitsu

引用次数: 0

Development and practical evaluation of an RT-PCR procedure using a real-time PCR instrument for saliva identification 利用实时荧光定量PCR仪进行唾液鉴定的RT-PCR程序的开发和实际评估

Japanese Journal of Forensic Science and Technology Pub Date : 2021-01-01 DOI: 10.3408/jafst.790

T. Akutsu, Ken Watanabe, Kochi Toyomane

{"title":"Development and practical evaluation of an RT-PCR procedure using a real-time PCR instrument for saliva identification","authors":"T. Akutsu, Ken Watanabe, Kochi Toyomane","doi":"10.3408/jafst.790","DOIUrl":"https://doi.org/10.3408/jafst.790","url":null,"abstract":" STAGE DOI: 10.3408 / jafst.790 ) Saliva is often left at crime scenes and its identiˆcation can prove useful in in-vestigating criminal cases such as sexual assault. In this study, we developed a rever-se-transcription polymerase chain reaction ( RT-PCR ) procedure for detection of STATH and HTN3 as markers characteristic of saliva, using the QuantStudio 5 real-time PCR system ( QS5 ) . Discrimination criteria were then proposed and evaluated on the speciˆcity, sensitivity, and applicability to forensic casework. The assay per-formance of QS5 was nearly identical to that of the SmartCycler II system ( SCII ) , which has been discontinued. Our proposed cutoŠ cycle quantiˆcation ( Cq ) values for the positive detection of saliva were Cq ＜ 40, 38, and 40 for ACTB , STATH , and HTN3 , respectively. The cutoŠ D Cq value for STATH was also set at 12. When the proposed criteria were applied, the developed procedure showed higher speciˆcity for saliva compared with conventional presumptive or conˆrmatory tests. Detection sensitivity was comparable to that of SCII but lower than that of a -amylase activity-based presumptive tests. An evaluation was then made using saliva samples under various storage conditions though Cq and D Cq values were drastically changed. In conclusion, the developed RT-PCR procedure has higher speciˆcity and lower sensitivity for saliva, suggesting its potential eŠectiveness for more precisely identifying saliva when performed in conjunction with current presumptive and conˆrmative saliva tests.","PeriodicalId":14709,"journal":{"name":"Japanese Journal of Forensic Science and Technology","volume":"61 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"80636530","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Expediting cannabis seed examination by combining color reaction and DNA testing 结合颜色反应和DNA检测，加快大麻种子检测

Japanese Journal of Forensic Science and Technology Pub Date : 2021-01-01 DOI: 10.3408/jafst.822

Tadashi Yamamuro, Yuki Okada, Hiroki Segawa, K. Kuwayama, K. Tsujikawa, T. Kanamori, Y. Iwata

{"title":"Expediting cannabis seed examination by combining color reaction and DNA testing","authors":"Tadashi Yamamuro, Yuki Okada, Hiroki Segawa, K. Kuwayama, K. Tsujikawa, T. Kanamori, Y. Iwata","doi":"10.3408/jafst.822","DOIUrl":"https://doi.org/10.3408/jafst.822","url":null,"abstract":"In Japan, possession of germinable cannabis seeds for cultivation purposes is subject to prosecution. Cannabis seeds are marketed after being treated to prevent germination ( heated or crushed ) Currently, forensic examination of cannabis seeds is conducted by cultivating the seeds after germination tests for several weeks and then performing morphological observation and chemical analysis on the true leaves. In this study, we attempted to construct a rapid and simple method for the identiˆcation of cannabis seeds by combining the color reaction using 2,3,5  Triphenyl  2 H  tetrazolium Chloride ( TTC ) , a reagent that discriminates between living and dead cells, and DNA testing using a commercially available simple kit. The color reaction using TTC can determine the viability of peeled embryos within 20 min at 45 ° C as previously reported. This method is eŠective for quickly determining whether a seed has been heat-treated or not. However, in the color reaction, a commercial health food seed that claimed to be unheated showed some colo-ration. This sample had been crushed to prevent germination and was easily identi-ˆed as non-germinable by morphological examination. After the color reaction, the embryos could be directly used for","PeriodicalId":14709,"journal":{"name":"Japanese Journal of Forensic Science and Technology","volume":"27 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"86813548","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0