Japanese Journal of Forensic Science and Technology最新文献_第5页

Characterization of the pull-up reduction feature on Data collection 4 in forensic STR typing 法医STR分型中数据收集4上拉约简特征的表征

Japanese Journal of Forensic Science and Technology Pub Date : 2021-01-01 DOI: 10.3408/jafst.787

Shota Inokuchi, N. Mizuno, K. Sekiguchi

引用次数: 0

Within-questionnaire consistency of the differential physiological responses in a Concealed Information Test: hierarchical Bayesian approach 隐信息测验中差异生理反应的问卷内一致性:层次贝叶斯方法

Japanese Journal of Forensic Science and Technology Pub Date : 2021-01-01 DOI: 10.3408/JAFST.794

Yusuke Shibuya

{"title":"Within-questionnaire consistency of the differential physiological responses in a Concealed Information Test: hierarchical Bayesian approach","authors":"Yusuke Shibuya","doi":"10.3408/JAFST.794","DOIUrl":"https://doi.org/10.3408/JAFST.794","url":null,"abstract":"10.3408 / jafst.794 ) The concealed information test ( CIT ) is a psychophysiological memory-detec-tion technique. The judgment to assess whether an examinee recognizes crime-relevant information is based on the diŠerence in autonomic responses between the crime-relevant information and the crime-irrelevant information. The present study examined the within-questionnaire consistency of the diŠerential physiological responses, which is a key point in the above judgment. The mock theft CIT data ob-tained from 167 participants were modeled into three possible models, and Bayesian estimation of parameters and model selection were performed. Results of model selection implied that participants in the encoding group were not clearly divided into high consistency responders and non-responders. Bayesian estimates of parameters revealed that the within-questionnaire consistency of the diŠerential physiological responses had diŠerent characteristics among the physiological measures. Most participants showed high consistency for heart rate and respiratory speed, while some participants show low consistency for skin conductance response, respiratory","PeriodicalId":14709,"journal":{"name":"Japanese Journal of Forensic Science and Technology","volume":"117 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"82063219","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Development of quantitative method for Δ9-THC in cannabis infused foods using GC-FID and QuEChERS 用GC-FID和QuEChERS建立大麻食品中Δ9-THC的定量方法

Japanese Journal of Forensic Science and Technology Pub Date : 2021-01-01 DOI: 10.3408/jafst.817

Katsumi Tachi, Kei Ieuji, Daisuke Kurakami, Yukari Tsumura

引用次数: 0

Evaluation of test performance of Simon's reagent and Marquis reagent for so-called Ecstasy tablets 对所谓摇头丸的西蒙试剂和马奎斯试剂测试性能的评价

Japanese Journal of Forensic Science and Technology Pub Date : 2021-01-01 DOI: 10.3408/jafst.791

K. Tsujikawa, Yuki Okada, Hiroki Segawa, Tadashi Yamamuro, K. Kuwayama, T. Kanamori, Y. Iwata, H. Inoue

引用次数: 0

Detection of ricin in beverages using the Bio-Threat Alert test strips 生物威胁预警试纸检测饮料中的蓖麻毒素

Japanese Journal of Forensic Science and Technology Pub Date : 2021-01-01 DOI: 10.3408/jafst.818

Mai Otsuka, H. Miyaguchi

{"title":"Detection of ricin in beverages using the Bio-Threat Alert test strips","authors":"Mai Otsuka, H. Miyaguchi","doi":"10.3408/jafst.818","DOIUrl":"https://doi.org/10.3408/jafst.818","url":null,"abstract":"Ricin, which is a proteinous toxin contained in castor beans, is one of the chemical warfare agents and strictly regulated by law. Regardless of the high toxicity, they have been used in some cases such as attempted murder cases due to the easy accessibility of castor beans. For these cases, detection of ricin is important for case control and proper remediation or decontamination. In this work, we have investigated the applicability of Bio-Threat Alert (BTA) test strips for the detection of ricin in beverages. By using BTA test strips, standard ricin protein in various beverages as low as 0.1 mg/mL could be detected by visual detection or by Guardian BTA Test Strip Reader. The calibration curves for ricin in tomato juice and cafe au lait were constructed using sample values obtained from Guardian BTA Test Strip Reader, which is the intensity of the color developed by the reaction of ricin and antibody. At high concentrations, the response seemed to be saturated. From the beverages added with mashed castor beans, ricin could also be detected by BTA test strips. Some beverages such as milk showed interfering behavior, but the reason seemed to not only come from the total protein amount but other factors.","PeriodicalId":14709,"journal":{"name":"Japanese Journal of Forensic Science and Technology","volume":"8 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"79714864","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Urinary excretion profiles of etizolam and its main metabolites after a single oral dose 单次口服依替唑仑及其主要代谢物的尿排泄特征

Japanese Journal of Forensic Science and Technology Pub Date : 2021-01-01 DOI: 10.3408/JAFST.801

Misato Wada, N. Shima, Tooru Kamata, S. Matsuta, Akari Ishikawa, Atsushi Nitta, Ryutaro Asai, Hidenao Kakehashi, Shihoko Fujii, K. Sasaki, H. Kamata, M. Katagi

引用次数: 0

Evaluation of a saliva presumptive test using the α-amylase assay kit 使用α-淀粉酶测定试剂盒评估唾液推定试验

Japanese Journal of Forensic Science and Technology Pub Date : 2021-01-01 DOI: 10.3408/JAFST.802

Jun Ohta, M. Ohmura, Saki Minegishi, Koichi Sakurada

引用次数: 0

Study of male DNA quantification methods in male-female mixture samples 男女混合样本中男性DNA定量方法的研究

Japanese Journal of Forensic Science and Technology Pub Date : 2021-01-01 DOI: 10.3408/JAFST.795

Sachi Tanaka, Jun Ohta, Kazuki Yamazaki

{"title":"Study of male DNA quantification methods in male-female mixture samples","authors":"Sachi Tanaka, Jun Ohta, Kazuki Yamazaki","doi":"10.3408/JAFST.795","DOIUrl":"https://doi.org/10.3408/JAFST.795","url":null,"abstract":"DNA quantiˆcation is an important process in DNA typing. Today, quantitative real-time PCR ( qPCR ) targeting the autosomal D17Z1 region, which cannot distinguish between male and female DNA, is the main method for forensic human DNA quantiˆcation for the Japanese police. For more e‹cient testing Y-STR type, we examined the forensic evaluation ( sensitivity, repeatability, speciˆcity, inhibi-tion, comparison with commercial qPCR reagent and simulated mixture study ) of qPCR assays targeting Y-chromosomal Sex determining region Y ( SRY ) and DYZ5 regions. We evaluated the eŠectiveness of simple and practical male DNA quantiˆcation methods in male and female mixture samples. The sensitivity of SRY and DYZ5 assays were 0.015 ng / m L and 0.0015 ng / m L. Both assays could quantify male DNA in the presence of high concentrations of female DNA. The SRY assay had the same sensitivity as the commercial qPCR reagent, but had more resistant to inhibitors. The DYZ5 assay tended to have higher DNA concentration than the commercial qPCR reagent and the SRY assay, but could detect trace amounts of male DNA. The reason for this is that DYZ5 region is multi-copy locus and there are individual diŠerences in the copy number. The DYZ5 assay was considered to be one of the eŠective methods for proving the presence of male DNA in the sample. Therefore, we consider that these assays can select the regions according to purpose and utilized to forensic applications.","PeriodicalId":14709,"journal":{"name":"Japanese Journal of Forensic Science and Technology","volume":"21 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"90053487","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Establishment of the new semen identification method and the examination to practical introduction 精液鉴定新方法的建立及检验方法的实用介绍

Japanese Journal of Forensic Science and Technology Pub Date : 2021-01-01 DOI: 10.3408/jafst.824

A. Sonoda, Ami Nagata, K. Tomonari, Takaaki Ono, Yoshito Tomisaka, Eiji Nishi

{"title":"Establishment of the new semen identification method and the examination to practical introduction","authors":"A. Sonoda, Ami Nagata, K. Tomonari, Takaaki Ono, Yoshito Tomisaka, Eiji Nishi","doi":"10.3408/jafst.824","DOIUrl":"https://doi.org/10.3408/jafst.824","url":null,"abstract":"Recently, AXO Science released STK Sperm Tracker Lab ( STK sheet ) and STK Sperm Tracker SPRAY ( STK spray ) to identify human semen stains. In this study, we examined the STK sheet and STK spray for their speciˆcity to detect human semen, sensitivity, eŠects on DNA, and response to old semen samples. Additionally, STK sheet was examined for optimal crimping time depending on the type of fabric. STK spray was examined through assessing the eŠect of ultraviolet ( UV ) radiation after spraying. As a result of this study, STK sheet and STK spray were found to ex-hibit high speciˆcity for the detection of human semen. These reagents did not aŠect the yield of extracted DNA or its degradation. STK sheet and STK spray can detect semen up to 1 / 25 th and 1 / 100 th dilutions, respectively. Both STK sheet and STK spray can react with semen samples left at room temperature for over a year. We found that the crimping time on STK sheet diŠers depending on the type of fabric, and the exposure to UV radiation for an hour after using STK spray does not aŠect DNA degradation. Conclusively, both STK sheet and STK spray are suitable to identify the human semen stains.","PeriodicalId":14709,"journal":{"name":"Japanese Journal of Forensic Science and Technology","volume":"23 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"80500033","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Full automation of sample pretreatment operations by supported liquid extraction for the determination of psychotropic drugs in blood 支持液体萃取法测定血液中精神药物样品前处理操作的全自动化

Japanese Journal of Forensic Science and Technology Pub Date : 2021-01-01 DOI: 10.3408/jafst.784

Maho Kawamura, Mitsuru Aizawa, Akihiro Nakamoto, A. Namera

引用次数: 0