Islets最新文献_第3页

Dual regulation of miR-375 and CREM genes in pancreatic beta cells 胰腺β细胞中miR-375和CREM基因的双重调控

IF 2.2 4区医学

Islets Pub Date : 2022-04-04 DOI: 10.1080/19382014.2022.2060688

D. Keller, Isis G. Perez

引用次数: 0

Modeling type 2 diabetes in rats by administering tacrolimus 他克莫司对大鼠2型糖尿病的影响

IF 2.2 4区医学

Islets Pub Date : 2022-03-29 DOI: 10.1080/19382014.2022.2051991

J. C. Quintana-Pérez, F. García-Dolores, A. S. Valdez-Guerrero, Diana Alemán-González-Duhart, M. Arellano-Mendoza, S. Rojas Hernández, I. Olivares-Corichi, J. R. García Sánchez, J. T. Trujillo Ferrara, F. Tamay-Cach

{"title":"Modeling type 2 diabetes in rats by administering tacrolimus","authors":"J. C. Quintana-Pérez, F. García-Dolores, A. S. Valdez-Guerrero, Diana Alemán-González-Duhart, M. Arellano-Mendoza, S. Rojas Hernández, I. Olivares-Corichi, J. R. García Sánchez, J. T. Trujillo Ferrara, F. Tamay-Cach","doi":"10.1080/19382014.2022.2051991","DOIUrl":"https://doi.org/10.1080/19382014.2022.2051991","url":null,"abstract":"ABSTRACT The prevalence of diabetes is rapidly increasing. The current number of diagnosed cases is ~422 million, expected to reach ~640 million by 2040. Type 2 diabetes, which constitutes ~95% of the cases, is characterized by insulin resistance and a progressive loss of β-cell function. Despite intense research efforts, no treatments are yet able to cure the disease or halt its progression. Since all existing animal models of type 2 diabetes have serious drawbacks, one is needed that represents the complete pathogenesis, is low cost and non-obese, and can be developed relatively quickly. The aim of this study was to evaluate a low-cost, non-obese model of type 2 diabetes engendered by administering a daily high dose of tacrolimus (an immunosuppressant) to Wistar rats for 4 weeks. The biochemical and antioxidant markers were measured at basal and after the 4-week tacrolimus treatment. At week 4, the values of these parameters closely resembled those observed in human type 2 diabetes, including fasting blood glucose at 141.5 mg/dL, blood glucose greater than 200 mg/dL at 120 min of the glucose tolerance test, blood glucose at varied levels in the insulin tolerance test, and elevated levels of cholesterol and triglyceride. The tacrolimus treatment produced hypoinsulinemia and sustained hyperglycemia, probably explained by the alteration found in pancreatic β-cell function and morphology. This model should certainly be instrumental for evaluating possible type 2 diabetes treatments, and for designing new immunosuppressants that do not cause pancreatic damage, type 2 diabetes, or new-onset diabetes after transplantation (NODAT).","PeriodicalId":14671,"journal":{"name":"Islets","volume":"14 1","pages":"114 - 127"},"PeriodicalIF":2.2,"publicationDate":"2022-03-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45875558","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 3

Determinants and dynamics of pancreatic islet architecture 胰岛结构的决定因素和动力学

IF 2.2 4区医学

Islets Pub Date : 2022-03-08 DOI: 10.1080/19382014.2022.2030649

Melissa T. Adams, B. Blum

引用次数: 15

Comparative analysis of reconstructed architectures from mice and human islets. 小鼠与人胰岛重构结构的比较分析。

IF 2.2 4区医学

Islets Pub Date : 2022-01-01 DOI: 10.1080/19382014.2021.1987827

Gerardo J Félix-Martínez, J R Godínez-Fernández

引用次数: 4

IsletLab: an application to reconstruct and analyze islet architectures. IsletLab:一个用于重建和分析岛屿结构的应用程序。

IF 2.2 4区医学

Islets Pub Date : 2022-01-01 DOI: 10.1080/19382014.2021.2008742

Gerardo J Félix-Martínez

引用次数: 2

Vesiculin derived from IGF-II drives increased islet cell mass in a mouse model of pre-diabetes. 来源于IGF-II的Vesiculin在糖尿病前期小鼠模型中驱动胰岛细胞质量增加。

IF 2.2 4区医学

Islets Pub Date : 2022-01-01 DOI: 10.1080/19382014.2021.1982326

Kate L Lee, Jacqueline F Aitken, Xun Li, Kirsten Montgomery, Huai-L Hsu, Geoffrey M Williams, Margaret A Brimble, Garth J S Cooper

{"title":"Vesiculin derived from IGF-II drives increased islet cell mass in a mouse model of pre-diabetes.","authors":"Kate L Lee, Jacqueline F Aitken, Xun Li, Kirsten Montgomery, Huai-L Hsu, Geoffrey M Williams, Margaret A Brimble, Garth J S Cooper","doi":"10.1080/19382014.2021.1982326","DOIUrl":"https://doi.org/10.1080/19382014.2021.1982326","url":null,"abstract":"Pancreatic islet-cell function and volume are both key determinants of the maintenance of metabolic health. Insulin resistance and islet-cell dysfunction often occur in the earlier stages of type 2 diabetes (T2D) progression. The ability of the islet cells to respond to insulin resistance by increasing hormone output accompanied by increased islet-cell volume is key to maintaining blood glucose control and preventing further disease progression. Eventual β-cell loss is the main driver of full-blown T2D and insulin-dependency. Researchers are targeting T2D with approaches that include those aimed at enhancing the function of the patient's existing β-cell population, or replacing islet β-cells. Another approach is to look for agents that enhance the natural capacity of the β-cell population to expand. Here we aimed to study the effects of a new putative β-cell growth factor on a mouse model of pre-diabetes. We asked whether: 1) 4-week's treatment with vesiculin, a two-chain peptide derived by processing from IGF-II, had any measurable effect on pre-diabetic mice vs vehicle; and 2) whether the effects were the same in non-diabetic littermate controls. Although treatment with vesiculin did not alter blood glucose levels over this time period, there was a doubling of the Proliferating Cell Nuclear Antigen (PCNA) detectable in the islets of treated pre-diabetic but not control mice and this was accompanied by increased insulin- and glucagon-positive stained areas in the pancreatic islets.","PeriodicalId":14671,"journal":{"name":"Islets","volume":"14 1","pages":"14-22"},"PeriodicalIF":2.2,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/33/83/KISL_14_1982326.PMC8632304.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10379915","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

An overview of current advancements in pancreatic islet transplantation into the omentum. 胰岛移植到网膜的最新进展概述。

IF 2.2 4区医学

Islets Pub Date : 2021-09-03 Epub Date: 2021-08-17 DOI: 10.1080/19382014.2021.1954459

Kimia Damyar, Vesta Farahmand, David Whaley, Michael Alexander, Jonathan R T Lakey

引用次数: 0

Frederick Banting's actual great idea: The role of fetal bovine islets in the discovery of insulin. 弗雷德里克·班廷的真正伟大的想法:胎牛胰岛在发现胰岛素中的作用。

IF 2.2 4区医学

Islets Pub Date : 2021-09-03 Epub Date: 2021-09-09 DOI: 10.1080/19382014.2021.1963188

James R Wright

{"title":"Frederick Banting's actual great idea: The role of fetal bovine islets in the discovery of insulin.","authors":"James R Wright","doi":"10.1080/19382014.2021.1963188","DOIUrl":"10.1080/19382014.2021.1963188","url":null,"abstract":"Background: Frederick Banting approached Toronto physiology professor JJR Macleod with a way to prevent pancreatic trypsin from destroying the pancreas' internal secretion. Banting proposed to induce exocrine atrophy by ligating canine pancreatic ducts and to use extracts of islet-rich residua to treat pancreatectomized dogs. His next plan was to make extracts from fetal pancreas, which he had read was islet-rich and lacked exocrine tissue capable of making trypsin; this work has not been historically evaluated.Methods: Banting's fetal calf pancreas story is told using primary and secondary historical sources and then critically examined using both historical and recent data on species phylogeny, islet ontogeny, fetal/neonatal islet culture/transplantation, etc. Results/Discussion: Only ruminants develop dual islets populations sequentially; fetal calf pancreata, at the gestational ages Banting used, possess numerous insulin-rich giant peri-lobular islets, which credibly explain the potency of his fetal calf insulin extract. Use of non-ruminant fetal pancreata would have failed.","PeriodicalId":14671,"journal":{"name":"Islets","volume":"13 5-6","pages":"121-133"},"PeriodicalIF":2.2,"publicationDate":"2021-09-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/48/6c/KISL_13_1963188.PMC8528409.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39398410","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 5

The Potential of Pancreatic Organoids for Diabetes Research and Therapy. 胰腺器官组织用于糖尿病研究和治疗的潜力

IF 1.9 4区医学

Islets Pub Date : 2021-09-03 Epub Date: 2021-09-15 DOI: 10.1080/19382014.2021.1941555

Katerina Bittenglova, David Habart, Frantisek Saudek, Tomas Koblas

{"title":"The Potential of Pancreatic Organoids for Diabetes Research and Therapy.","authors":"Katerina Bittenglova, David Habart, Frantisek Saudek, Tomas Koblas","doi":"10.1080/19382014.2021.1941555","DOIUrl":"10.1080/19382014.2021.1941555","url":null,"abstract":"The success of clinical transplantation of pancreas or isolated pancreatic islets supports the concept of cell-based cure for diabetes. One limitation is the shortage of cadaver human pancreata. The demand-supply gap could potentially be bridged by harnessing the self-renewal capacity of stem cells. Pluripotent stem cells and adult pancreatic stem cells have been explored as possible cell sources. Recently, a system for long-term culture of proposed adult pancreatic stem cells in a form of organoids was developed. Generated organoids partially mimic the architecture and cell-type composition of pancreatic tissue. Here, we review the attempts over the past decade, to utilize the organoid cell culture principles in order to identify, expand, and differentiate the adult pancreatic stem cells from different compartments of mouse and human pancreata. The development of the culture conditions, effects of specific growth factors and small molecules is discussed. The potential utility of the adult pancreatic stem cells is considered in the context of other cell sources.","PeriodicalId":14671,"journal":{"name":"Islets","volume":"13 5-6","pages":"85-105"},"PeriodicalIF":1.9,"publicationDate":"2021-09-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8528407/pdf/KISL_13_1941555.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39417048","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Hnf1b-CreER causes efficient recombination of a Rosa26-RFP reporter in duct and islet δ cells. Hnf1b-CreER在导管和胰岛δ细胞中引起Rosa26-RFP报告基因的有效重组。

IF 2.2 4区医学

Islets Pub Date : 2021-09-03 Epub Date: 2021-07-20 DOI: 10.1080/19382014.2021.1955088

Meritxell Rovira, Miguel Angel Maestro, Vanessa Grau, Jorge Ferrer

{"title":"Hnf1b-CreER causes efficient recombination of a Rosa26-RFP reporter in duct and islet δ cells.","authors":"Meritxell Rovira, Miguel Angel Maestro, Vanessa Grau, Jorge Ferrer","doi":"10.1080/19382014.2021.1955088","DOIUrl":"https://doi.org/10.1080/19382014.2021.1955088","url":null,"abstract":"The Hnf1b-CreERT2 BAC transgenic (Tg(Hnf1b-cre/ERT2)1Jfer) has been used extensively to trace the progeny of pancreatic ducts in developmental, regeneration, or cancer models. Hnf1b-CreERT2 transgenics have been used to show that the cells that form the embryonic pancreas duct-like plexus are bipotent duct-endocrine progenitors, whereas adult mouse duct cells are not a common source of β cells in various regenerative settings. The interpretation of such genetic lineage tracing studies is critically dependent on a correct understanding of the cell type specificity of recombinase activity with each reporter system. We have reexamined the performance of Hnf1b-CreERT2 with a Rosa26-RFP reporter transgene. This showed inducible recombination of up to 96% adult duct cells, a much higher efficiency than previously used reporter transgenes. Despite this high duct-cell excision, recombination in α and β cells remained very low, similar to previously used reporters. However, nearly half of somatostatin-expressing δ cells showed reporter activation, which was due to Cre expression in δ cells rather than to duct to δ cell conversions. The high recombination efficiency in duct cells indicates that the Hnf1b-CreERT2 model can be useful for both ductal fate mapping and genetic inactivation studies. The recombination in δ cells does not modify the interpretation of studies that failed to show duct conversions to other cell types, but needs to be considered if this model is used in studies that aim to modify the plasticity of pancreatic duct cells.","PeriodicalId":14671,"journal":{"name":"Islets","volume":"13 5-6","pages":"134-139"},"PeriodicalIF":2.2,"publicationDate":"2021-09-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1080/19382014.2021.1955088","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39202054","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 3