Iranian Journal of Otorhinolaryngology最新文献

{"title":"Differentiated Thyroid Cancer Recurrence and Mortality Prognostic Factors: A Retrospective Cohort Study on 350 Adult Patients.","authors":"Hosein Rabiei, Amin Doosti-Irani, Shiva Borzouei","doi":"10.22038/ijorl.2026.93088.4094","DOIUrl":"10.22038/ijorl.2026.93088.4094","url":null,"abstract":"<p><strong>Introduction: </strong>Differentiated thyroid cancer (DTC) accounts for the most common types of thyroid cancer. The aim of the present study was to investigate the factors associated with recurrence and mortality after thyroidectomy in patients with diagnose of DTC in Hamadan, Iran.</p><p><strong>Materials and methods: </strong>In this retrospective cohort study, 350 patients over 18 years old who underwent total thyroidectomy and ablation with radioactive iodine (RAI) and had DTC based on pathology and followed up for ten years were studied. The univariate and multivariate logistic regression was used for determining the risk factors of recurrence and mortality of DTC at a significance level of 0.05. SPSS software (Version 26) was applied to analyze the data.</p><p><strong>Results: </strong>In the present study, 16.6% of the participants were male and 83.4% were female with a mean age of 40.7 ±14.7 years. Recurrence and mortality rates were obtained to be 26.9% and 4.9 %, respectively. Overall, the risk of recurrence and death was significantly higher in older age, men, smokers, tumors larger than 4 cm and regional lymph node (p<0.05). For each additional year of life, the risk of recurrence and death increased by 4% and 9%, respectively (P =0.001 and p<0.001). Recurrence in men were 5.111 times more likely than women (p<0.001). A 6.002-fold increase in the risk of death was associated with smoking (P=0.008).</p><p><strong>Conclusion: </strong>Due to the high prevalence of DTC and on the other hand the good prognosis of this type of cancer, timely diagnosis and identification of risk factors of recurrence and death are very important.</p>","PeriodicalId":14607,"journal":{"name":"Iranian Journal of Otorhinolaryngology","volume":"38 3","pages":"179-187"},"PeriodicalIF":0.0,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13459123/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148712516","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Efficacy of Intratympanic versus Systemic Corticosteroids in Sudden Sensorineural Hearing Loss: A Systematic Review.","authors":"Mateo Andrés Leal Capacho, Laura Tatiana León Suárez, Merly Lilian Figueroa Márquez, Jessica Lizeth Cuaspa Damian, Juan Pablo Alzate Granado, Francisco Javier Gómez Ballesta","doi":"10.22038/ijorl.2026.91544.4050","DOIUrl":"10.22038/ijorl.2026.91544.4050","url":null,"abstract":"<p><strong>Introduction: </strong>Sudden sensorineural hearing loss (SSNHL) is an otologic emergency that typically requires prompt treatment with corticosteroids, administered either systemically or intratympanically. The optimal route for initial therapy remains uncertain due to heterogeneity in efficacy and safety profiles.</p><p><strong>Materials and methods: </strong>We conducted a systematic review of randomized controlled trials (RCTs) published between 2011 and 2025, comparing intratympanic and systemic corticosteroids as primary treatment for SSNHL in adults. Following PRISMA 2020 and Cochrane Handbook guidelines, we screened studies without language restrictions. Two independent reviewers performed study selection, data extraction, and risk of bias assessment using the RoB 2.0 tool. Outcomes were synthesized narratively, focusing on changes in pure-tone average (PTA), complete/functional recovery, speech discrimination, and safety. Trials with combination/salvage strategies, adjuncts, or formulation comparisons were synthesized separately.</p><p><strong>Results: </strong>Seventeen RCTs were included. Efficacy results were heterogeneous; however, most studies indicated that intratympanic corticosteroids are at least as effective as systemic therapy. In severe hearing loss, intratympanic treatment achieved higher success rates (up to 70.6%) and greater PTA improvement compared to systemic administration. Adverse events differed by route: systemic corticosteroids were associated with metabolic disturbances, while intratympanic therapy caused localized, self-limiting events. Overall, 47.1% of studies had high risk of bias, and 52.9% showed some concerns.</p><p><strong>Conclusion: </strong>Intratympanic corticosteroids appear to be a non-inferior alternative to systemic steroids for initial SSNHL treatment, with potential advantages in cases of severe hearing loss or systemic contraindications. Nonetheless, methodological limitations across studies warrant cautious interpretation. Future high-quality trials should address efficacy, functional recovery, and safety more rigorously.</p>","PeriodicalId":14607,"journal":{"name":"Iranian Journal of Otorhinolaryngology","volume":"38 3","pages":"137-148"},"PeriodicalIF":0.0,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13459104/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148711961","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comparison of Mechanical Properties of Nasal Cartilage Preserved in Ethanol at 4 °C versus Normal Saline at -18 °C for Rhinoplasty Reuse.","authors":"Saeid Atighechi, Fatemeh Jabinian, Zahra Momenramazani, Saeed Hasani, Sedighe Vaziribozorg","doi":"10.22038/ijorl.2026.92496.4079","DOIUrl":"10.22038/ijorl.2026.92496.4079","url":null,"abstract":"<p><strong>Introduction: </strong>This study aimed to compare the mechanical properties of nasal cartilage preserved in ethanol at 4 °C versus normal saline at -18 °C, with the goal of assessing their suitability for reuse in subsequent nasal surgeries.</p><p><strong>Materials and methods: </strong>Twenty nasal septal cartilage samples from patients who had undergone rhinoplasty were preserved for two months using two different methods. Ten cartilages were stored in alcohol at 4 °C, while the remaining ten were stored in normal saline at -18 °C. Additionally, a control group consisting of ten fresh cartilage samples was included for comparison. The mechanical properties (namely Young's modulus, fracture stress, yield stress, ductility, and tensile strength) were measured using a universal tensile testing machine STM-20 and analyzed to evaluate the effects of the preservation methods.</p><p><strong>Results: </strong>Significant differences were observed between the groups in mean tensile strength, ductility, Young's modulus, and yield stress (P<0.05). However, no significant difference was found in mean fracture stress (P=0.318). The alcohol-preserved group exhibited significantly lower mechanical properties compared to the saline and fresh cartilage groups (P<0.05). No significant differences were detected between the frozen (saline) and fresh cartilage groups (P>0.05).</p><p><strong>Conclusions: </strong>Cartilage stored in normal saline at -18 °C retained its mechanical properties better than cartilage preserved in alcohol at 4 °C. Freezing did not adversely affect the mechanical properties, and the slightly higher values observed in the frozen group suggest that lower temperature may enhance cartilage elasticity.</p>","PeriodicalId":14607,"journal":{"name":"Iranian Journal of Otorhinolaryngology","volume":"38 4","pages":"247-258"},"PeriodicalIF":0.0,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13495739/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148793210","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Nasopharyngeal Solitary Extramedullary Plasmacytoma: A Rare Case and Review of Literature.","authors":"Shivangi Patnecha, Rupa Mehta, Siddhartha Nanda, Amit Chowhan","doi":"10.22038/ijorl.2025.88907.3981","DOIUrl":"10.22038/ijorl.2025.88907.3981","url":null,"abstract":"<p><strong>Introduction: </strong>Solitary extramedullary plasmacytoma (EMP) of the nasopharynx is a rare plasma cell neoplasm, representing a small subset of head and neck malignancies. Its nonspecific symptoms often lead to diagnostic delays.</p><p><strong>Case report: </strong>A 50-year-old female with non-specific progressive nasal obstruction and intermittent epistaxis, underwent complete endoscopic surgical excision using coblation. Post operative histopathology revealed Nasopharyngeal Extramedullary Plasmacytoma. The patient then received adjuvant radiotherapy (Volumetric Modulated Arc Therapy - VMAT), leading to a successful outcome with no recurrence at the one-year follow-up.</p><p><strong>Conclusion: </strong>This case underscores the importance of clinico-histopathological evaluation and a comprehensive, multimodal treatment approach, with nasopharyngeal extramedullary plasmacytoma (EMP) considered as a differential diagnosis in nasopharyngeal masses. Given the potential for progression to multiple myeloma, long-term surveillance is crucial. Increased awareness of this rare entity can facilitate early diagnosis, timely intervention, and improved patient outcomes.</p>","PeriodicalId":14607,"journal":{"name":"Iranian Journal of Otorhinolaryngology","volume":"38 2","pages":"131-136"},"PeriodicalIF":0.0,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13090810/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147722565","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Mechanistic Insights into the Role of Adenoid Hypertrophy and Chronic Adenoiditis in the Pathogenesis of Pediatric Chronic Rhinosinusitis: A Systematic Review.","authors":"Gagat Ragil Andaru Pratomo, Feri Trihandoko","doi":"10.22038/ijorl.2026.97252.4200","DOIUrl":"10.22038/ijorl.2026.97252.4200","url":null,"abstract":"<p><strong>Objective: </strong>To synthesize available evidence on the mechanistic role of adenoid hypertrophy and chronic adenoiditis in the pathogenesis of pediatric chronic rhinosinusitis (CRS), given that the mechanistic relationship between adenoid disease and pediatric CRS remains incompletely synthesized.</p><p><strong>Data sources: </strong>PubMed/MEDLINE, Embase, Scopus, Web of Science Core Collection, and Cochrane Library were systematically searched in accordance with PRISMA 2020 guidelines.</p><p><strong>Methods of review: </strong>Eligible studies included children with CRS and evaluated adenoid- related mechanisms, including mechanical obstruction, impaired drainage, microbial reservoir function, biofilm formation, immune dysregulation, epithelial dysfunction, mucociliary impairment, or clinical outcomes after adenoidectomy. Study quality was appraised using the JBI Critical Appraisal Tools. Due to heterogeneity in study design, diagnostic criteria, and reported outcomes, a narrative synthesis was performed.</p><p><strong>Results: </strong>Nine studies involving 927 pediatric participants were included. Evidence for mechanical obstruction was mixed, as adenoid size did not consistently correlate with CRS severity. Stronger evidence supported the role of the adenoid as a microbial reservoir and biofilm- bearing tissue, with studies reporting pathogenic bacterial colonization and extensive adenoidal biofilm in children with CRS. Limited molecular evidence suggested reduced surfactant protein- D expression, while epithelial barrier dysfunction and mucociliary impairment were supported mainly by indirect findings. Adenoidectomy improved symptoms and quality of life in selected children with medically refractory CRS.</p><p><strong>Conclusion: </strong>Adenoid hypertrophy and chronic adenoiditis appear to be associated with pediatric CRS through combined obstructive, microbial, biofilm- related, inflammatory, and immunological pathways, although causal relationships cannot be established from the available evidence. Adenoidectomy appears beneficial in selected refractory cases, although further prospective mechanistic studies are needed.</p>","PeriodicalId":14607,"journal":{"name":"Iranian Journal of Otorhinolaryngology","volume":"38 5","pages":"289-300"},"PeriodicalIF":0.0,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13589882/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149012047","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Usefulness of Reflux Symptom Index and Reflux Finding Score in the diagnosis of Laryngopharyngeal Reflux Disease.","authors":"Probal Chatterji, Yashodeep Mukherjee","doi":"10.22038/ijorl.2026.93713.4111","DOIUrl":"10.22038/ijorl.2026.93713.4111","url":null,"abstract":"<p><strong>Introduction: </strong>Laryngopharyngeal reflux disease (LPRD) is a common condition with an estimated prevalence between 8-30% in the general population. The complaints of LPRD are non-specific and may overlap with many other common conditions leading to misdiagnosis. Reflux Symptom Index (RSI) is a 9-item patient response based questionnaire developed to diagnose LPRD. Similarly, Reflux Finding Score (RFS) is an 8-item based scoring system created to diagnose LPRD based on endoscopic findings during laryngoscopy. The aim of our study was to evaluate the usefulness of the two scoring systems in identifying patients with LPRD.</p><p><strong>Materials and methods: </strong>Patients with features suggestive of LPRD were considered for participation as per strict inclusion and exclusion criteria. The RSI and RFS was calculated in each case and result was analyzed for statistical significance.</p><p><strong>Results: </strong>Total of 150 patients were identified with 37.38 years as mean age of presentation. M:F ratio was 1:1.88. 111 cases (74%) had RSI score above 13 suggestive of LPRD with mean RSI of 19.58. 84 cases (56%) had RFS above 7 suggestive of LPRD with mean RFS of 7.29. There was a positive correlation between RSI score and RFS.</p><p><strong>Conclusion: </strong>There was positive correlation between RSI score and RFS in our study (p<0.0001) and the correlation was stronger with RSI scores above 13. This corroborates with the findings of most studies although some authors have inferred otherwise. Using both RSI score and RFS may be useful and complimentary to each other for the correct diagnosis of LPRD.</p>","PeriodicalId":14607,"journal":{"name":"Iranian Journal of Otorhinolaryngology","volume":"38 5","pages":"333-340"},"PeriodicalIF":0.0,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13589890/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149012195","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Blast-Induced Auditory Injury: From Peripheral Pathology to Central Neurodegeneration.","authors":"Maryam Ramezani, Seyedeh Nazanin Hajjari","doi":"10.22038/ijorl.2026.93174.4098","DOIUrl":"https://doi.org/10.22038/ijorl.2026.93174.4098","url":null,"abstract":"<p><strong>Introduction: </strong>Blast wave exposure represents a primary cause of complex auditory and neurological injuries. The ear acts as a sensitive pressure transducer, making it exceptionally vulnerable to the intense mechanical forces of a blast. This review provides an overview of the multidimensional damage to the auditory system following blast events.</p><p><strong>Materials and methods: </strong>We conducted a systematic literature search across PubMed, Scopus, Web of Science, and Google Scholar (January 2000-January 2025) using comprehensive search terms. After screening 462 unique records, 36 original research studies (27 animal, 8 human, 1 computational) met the inclusion criteria and underwent quality assessment.</p><p><strong>Results: </strong>Evidence suggests that blast exposure causes widespread auditory impairment, ranging from cochlear synaptopathy to central neurodegeneration. Blast-related injuries involve distinct biomechanical forces that differ from conventional noise trauma. Furthermore, cumulative damage from repeated exposures leads to chronic functional impairments, including tinnitus and difficulty understanding speech in noisy environments.</p><p><strong>Conclusion: </strong>Effective management of blast-induced hearing loss requires a multidisciplinary approach. Future research needs to focus on validating sensitive biomarkers for early diagnosis and developing targeted neuroprotective therapies.</p>","PeriodicalId":14607,"journal":{"name":"Iranian Journal of Otorhinolaryngology","volume":"38 4","pages":"209-221"},"PeriodicalIF":0.0,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13474594/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148760225","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effectiveness of Superior Laryngeal Nerve Block in Patients with Neurogenic Cough: A Prospective Cohort Study.","authors":"Jasmeet Kaur, Vishav Yadav","doi":"10.22038/ijorl.2026.95660.4148","DOIUrl":"10.22038/ijorl.2026.95660.4148","url":null,"abstract":"<p><strong>Introduction: </strong>A study was undertaken to evaluate the role of superior laryngeal nerve block in management of neurogenic cough.</p><p><strong>Materials and methods: </strong>A Prospective Cohort study was conducted on 30 patients of neurogenic cough in Government Medical College, Patiala, India from Jan 2024 to May 2025. Adult patients with unexplained cough > 8wks were included in the study and superior laryngeal nerve block with long acting steroid and local anaesthetic was given. All patients were monitored every two weeks for three months post superior laryngeal nerve block. Pre and post treatment cough severity index (CSI) were compared.</p><p><strong>Results: </strong>In our study of thirty patients, twenty five patients showed subjective symptomatic improvement. Normality of continuous data was assessed using the Shapiro Wilk test. For Intra comparison Paired t- test was used. The mean cough severity index (CSI) score decreased significantly from 24.533 ± 2.909 to 16.167 ± 2.365 post intervention with p value 0.001 which was found to be highly significant.</p><p><strong>Conclusion: </strong>SLN block is an effective alternative therapeutic option for patients of neurogenic cough in terms of efficacy, minimal invasiveness and safety.</p>","PeriodicalId":14607,"journal":{"name":"Iranian Journal of Otorhinolaryngology","volume":"38 5","pages":"311-315"},"PeriodicalIF":0.0,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13589888/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149011957","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comparison of the Neutrophil-to-Lymphocyte and Platelet-to-Lymphocyte Ratios in Two Groups of Patients with Benign and Malignant Salivary Gland Tumors.","authors":"Mohammad Hossein Shafieyoun, Nezamoddin Berjis, Afrooz Eshaghian","doi":"10.22038/ijorl.2025.89668.4001","DOIUrl":"10.22038/ijorl.2025.89668.4001","url":null,"abstract":"<p><strong>Introduction: </strong>The ratio of neutrophils to peripheral lymphocytes is an inflammatory marker, and, based on previous studies, this ratio is associated with poor survival in several cancers. This study was conducted to compare the neutrophils-to-lymphocytes ratio (NLR) and platelets-to-lymphocytes ratio (PLR) in two groups of patients with benign and malignant salivary gland tumors.</p><p><strong>Materials and methods: </strong>This study was cross-sectional, comparing two groups of patients with benign and malignant salivary gland masses. During the last 6 years (from 2018 to 2024), 380 Patients with a definite diagnosis of salivary gland tumors were included in the study, and NLR and PLR data were obtained from pre-operation laboratory exams.</p><p><strong>Results: </strong>NLR and PLR were significantly higher on average than in benign masses (P<0.05). The best cut point was 2.24 for NLR (sensitivity: 78%, specificity: 80%) and 104.35 for PLR (sensitivity: 68%, specificity: 60%).</p><p><strong>Conclusion: </strong>NLR and PLR are easy, practical methods that provide valuable information for diagnosing, assessing severity, and predicting prognosis of various diseases, such as salivary gland masses.</p>","PeriodicalId":14607,"journal":{"name":"Iranian Journal of Otorhinolaryngology","volume":"38 1","pages":"29-35"},"PeriodicalIF":0.0,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12828120/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146051998","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The Effect of Culture Medium Obtained from Dental Pulp Stem Cells (Conditioned Medium) on Esophageal Squamous Cell Carcinoma Cell Line (KYSE-30).","authors":"Setare Tavakoli, Akbar Safipour Afshar, Nooshin Mohtasham, Zohre Samiee, Mohammad Amin Yaghoubi, Farnaz Mohajertehran","doi":"10.22038/ijorl.2025.84920.3857","DOIUrl":"10.22038/ijorl.2025.84920.3857","url":null,"abstract":"<p><strong>Introduction: </strong>Esophageal squamous cell carcinoma (ESCC) is the eighth most prevalent cancer worldwide. Head and neck squamous cell carcinoma (HNSCC) represents over 90% of all head and neck cancers, and nearly 40% of patients fail treatment. Therefore, discussion of cancer treatment is essential. In this in vitro study, we examined how conditioned medium from dental pulp stem cells (DPSC-CM) influences the ESCC cell line (KYSE-30).</p><p><strong>Materials and methods: </strong>First, the middle pulp tissue of the wisdom tooth was extracted and, following sterilization, transferred to a cell culture flask containing MEM-α. After completing the culture procedures, conditioned medium (CM) was collected from the fourth passage culture after 72 hours in serum-free medium. The KYSE-30 cells were then treated with the CM for an additional 72 hours, and cell survival was assessed using the MTT assay. Statistical significance was evaluated using one-way ANOVA followed by Tukey's post-hoc test.</p><p><strong>Results: </strong>The results demonstrated that treatment of esophageal cancer cells with conditioned medium (CM) significantly reduced the survival rate of cancer cells compared to control samples. The treatment of KYSE-30 cells with DPSC-CM led to a notable reduction in cell viability (CM group: 51.2 ± 4.1% vs. control group: 72.6 ± 3.3%; P = 0.004).</p><p><strong>Conclusion: </strong>DPSC-CM demonstrates the ability to reduce the proliferation of cancer cells; therefore, this medium can be considered a potential drug (therapeutic) candidate for the treatment of esophageal cancer. However, further studies are required to confirm these results.</p>","PeriodicalId":14607,"journal":{"name":"Iranian Journal of Otorhinolaryngology","volume":"38 1","pages":"11-17"},"PeriodicalIF":0.0,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12828138/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146052057","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}