Iranian South Medical Journal最新文献_第10页

Molecular Typing of Mycobacterium Tuberculosis Isolated from Iranian Patients Using Highly Abundant Polymorphic GC-Rich-Repetitive Sequence 利用高丰态gc - rich - repeat序列对伊朗结核分枝杆菌进行分子分型

Iranian South Medical Journal Pub Date : 2020-06-01 DOI: 10.52547/ismj.23.2.87

B. G. Eimani, K. Ansarin, L. Sahebi, M. Seyyedi

{"title":"Molecular Typing of Mycobacterium Tuberculosis Isolated from Iranian Patients Using Highly Abundant Polymorphic GC-Rich-Repetitive Sequence","authors":"B. G. Eimani, K. Ansarin, L. Sahebi, M. Seyyedi","doi":"10.52547/ismj.23.2.87","DOIUrl":"https://doi.org/10.52547/ismj.23.2.87","url":null,"abstract":"Background: Tuberculosis (TB) with more than 10 million new cases per year and one of the top 10 causes of death worldwide, is still one of the most important global health problems. Also, multi drug-resistant tuberculosis (MDR) is a serious danger to public health. Understanding of the epidemiological pattern of mycobacterium tuberculosis (MTB), Estimates of recent transmission and recurrence of infection, it is possible with molecular typing methods. The present study was performed aiming to track and determine the type of Mycobacterium tuberculosis infection, as well as its relationship with demographic factors, using PGRS-RFLP. Materials and Methods: In this study, 90 samples collected from TB patients from the North West provinces of Iran, Molecular typing by Characterization highly abundant polymorphic GC-rich-repetitive sequence. Investigated Demographic factors associated with the transmission of the disease. Results: All isolates were grouped into 44 clusters 28types (33.3%) of the subspecies were in unique stains and 66.7% (from 56 patients) had clustered isolates. The largest cluster contained 8 isolates (9.52%) was the West provinces of Iran. Conclusion: Genetic variation of Mycobacterium tuberculosis is high in this region. The rate of recent transmission based on clustering was unexpected (The global average is 30%-40%). The recent transmission was more dynamic in the west than northwest Iran. Clustering based on PGRS-RFLP can demonstrate the high correlation between molecular and classic information. In addition, the significant relationship between vaccination record and clustering highlights the necessity to conduct more extensive studies.","PeriodicalId":14583,"journal":{"name":"Iranian South Medical Journal","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2020-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43802131","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Evaluation of Lethal Effect of Curcumin and its Derivatives Against Leishmania Major In Vitro 姜黄素及其衍生物对利什曼原虫体外杀伤作用的评价

Iranian South Medical Journal Pub Date : 2020-06-01 DOI: 10.52547/ismj.23.2.153

M. Fouladvand, Solyman Khorami, Behroz Naeimi, Sohila Fotouhi, Khosro Mohammadi

{"title":"Evaluation of Lethal Effect of Curcumin and its Derivatives Against Leishmania Major In Vitro","authors":"M. Fouladvand, Solyman Khorami, Behroz Naeimi, Sohila Fotouhi, Khosro Mohammadi","doi":"10.52547/ismj.23.2.153","DOIUrl":"https://doi.org/10.52547/ismj.23.2.153","url":null,"abstract":"Background: Leishmania major is transmitted by sandflies of the genus Phobotomus and causes cutaneous lesions in humans. Curcumin is made from turmeric and various derivatives is derived from it. In the present study the effect of curcumin and it’s derivatives on Leishmania major in vitro was investigated. Materials and Methods: Curcumin 70% and 90% purity and it’ derivatives such as s , BDMC base methoxy curcumin, diacetyl curcumin DAC, vanadyl curcumin VO (CUR) 2, vanadyl diacetyl curcumin VO (DAC) 2, indium curcumin In(CUR)3 and Ga (CUR) 3 preparation. Curcumin derivatives were synthesized and different concentrations of 500 to 800 μg/ ml were prepared in glycerin. 106 Leishmania major promastigotes added to each well of 96 well plate and 100 µL of each curcumin derivative added and stored at 25 ° C for 24 hours. Assay of the lethality of the compounds against Leishmania major and evaluation of their toxic effects on (vero) cells were evaluated using MTT assay. Results: Lethal effect of curcumin and it’s derivatives against Leishmania, increased with increasing concentration. Anti-leishmanial index (IC 50 ) of 70% and 90% curcumin compounds, (BDMC) bis methoxy curcumin, Diacetyl curcumin (DAC), vanadyl curcumin VO (CUR)2, vanadyl diacetyl curcumin VO (DAC)2, indium curcumin In (CUR)3, Gallium Curcumin Ga (CUR)3 compounds were 110, 93, 101, 103, 98, 103, 51 and 58 µg / ml and the lethal effect of these compounds against (vero) cells were 33.1%, 19%, 21%, 20.3%, 17%, 21%, 25.3%, 16%, respectively. Conclusion: Gallium curcumin and indium curcumin, compared to curcumin and other derivatives, exhibited the highest anti-leishmanial effect and were the safest derivatives of curcumin for mammalian cells.","PeriodicalId":14583,"journal":{"name":"Iranian South Medical Journal","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2020-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41627390","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Comparative Evaluation of Expression Vectors (pET32a and pET25b) in Expression of Polyepitopic Sequence Synthesized from Leishmania infantum Antigens 表达载体pET32a和pET25b表达婴儿利什曼原虫抗原合成的聚皮异位序列的比较评价

Iranian South Medical Journal Pub Date : 2020-06-01 DOI: 10.52547/ismj.23.2.143

Marzeah Taherzadeh, M. Fouladvand, B. Kazemi

{"title":"Comparative Evaluation of Expression Vectors (pET32a and pET25b) in Expression of Polyepitopic Sequence Synthesized from \u0000Leishmania infantum Antigens","authors":"Marzeah Taherzadeh, M. Fouladvand, B. Kazemi","doi":"10.52547/ismj.23.2.143","DOIUrl":"https://doi.org/10.52547/ismj.23.2.143","url":null,"abstract":"Background: Molecular methods are nowadays used to diagnose diseases, produce vaccines, drugs and recombinant proteins. Therefore, one of the important steps in these procedures is selecting the appropriate vector for cloning and expression of the target genes. Because the expression of synthetic polygenes or so-called chimeric antigens with high molecular weight requires special conditions, the aim of this study was to evaluate the importance of vector type for expression of such antigens. Materials and Methods: The 1250bp sequence consisting of epitopes from eight important antigens of Leishmania infantum parasite was designed and synthesized by Biomatic Company (Cambridge, Canada). The sequence was cloned separately in two expression vectors pET25b and pET32a and then transformed into E.coli BL21 (DE3) and expressed under similar appropriate conditions. Bacterial lysates were analyzed by SDS PAGE and evaluated by Western blotting. Results: of SDS- PAGE and Western blot analysis showed that intact recombinant protein production by pET25b vector was not successful. However, the recombinant protein resulted from the expression of the aforementioned poly-epitope in pET32a vector was successfully produced and confirmed. Conclusion: According to the successful expression confirmation of the poly-epitopic sequence within the pET32a vector and furthermore the failure to obtain protein in the pET25b vector showed that in the case of some specific mosaic sequences expression, protein isolation will be difficult because of low solubility. So, the expression vector choose should be made more carefully.","PeriodicalId":14583,"journal":{"name":"Iranian South Medical Journal","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2020-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46180139","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Magnetic Separation of Giardia Cysts from Water 贾第鞭毛虫囊从水中磁分离

Iranian South Medical Journal Pub Date : 2020-06-01 DOI: 10.52547/ismj.23.2.99

R. Ghasemikhah, Zainab Davudi Moghadam, F. Ahmadi, Mahsa Rasulpur, M. Bayatiani, R. Hajihossein, H. Sarmadian, F. Seif

{"title":"Magnetic Separation of Giardia Cysts from Water","authors":"R. Ghasemikhah, Zainab Davudi Moghadam, F. Ahmadi, Mahsa Rasulpur, M. Bayatiani, R. Hajihossein, H. Sarmadian, F. Seif","doi":"10.52547/ismj.23.2.99","DOIUrl":"https://doi.org/10.52547/ismj.23.2.99","url":null,"abstract":"Background: Giardia is one of the infectious agents that can be transmitted by surface water and cause a disease. We designed and fabricated a magnetic system to separate the giardia cysts from water. Materials and Methods: In this study, giardia cysts were concentrated by sucrose floatation method. The cysts were mixed with physiological saline and their concentration was adjusted to 100,000 cysts per cubic millimeter using Neubauer slide. The isolated cysts were passed through the magnetic field generated by the 200-msT magnets. The cysts were attracted to the magnet and removed from the suspension. The cysts were exposed to magnetic field for one week in order to evaluate the effect of magnetic field on their survival, which was reported as 0.1% using eosin staining under a light microscope. Results: The results showed that magnetic field can relatively absorb giardia cysts from water, the survival rate of the cysts did not change significantly in different days (P≥0.05). Conclusion: The designed magnetic system can be reinforced to isolate giardia cysts, which is economical and has no chemical effects on water.","PeriodicalId":14583,"journal":{"name":"Iranian South Medical Journal","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2020-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48883466","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The Fifth Generation University: Based on the Quintuple Helix of Carayannis and Campbell 第五代大学:基于Carayannis和Campbell的五重螺旋

Iranian South Medical Journal Pub Date : 2020-06-01 DOI: 10.52547/ismj.23.2.165

I. Nabipour

引用次数: 3

Predictor Factors of Recycling Behavior in Residents of Bushehr City: Application of Theo-ry of Planned Behavior 布什尔市居民回收行为的预测因素:计划行为理论的应用

Iranian South Medical Journal Pub Date : 2020-06-01 DOI: 10.52547/ismj.23.2.116

Sahar Haghighatjoo, Aida Asgari, R. Tahmasebi, A. Noroozi

{"title":"Predictor Factors of Recycling Behavior in Residents of Bushehr City: Application of Theo-ry of Planned Behavior","authors":"Sahar Haghighatjoo, Aida Asgari, R. Tahmasebi, A. Noroozi","doi":"10.52547/ismj.23.2.116","DOIUrl":"https://doi.org/10.52547/ismj.23.2.116","url":null,"abstract":"Background: Recycling is one of the most important measures to manage the municipal solid waste. In order to design a training program, it is necessary to identify the factors that influence behavior. Therefore, the present study aimed to investigate the predictive factors of recycling behavior among residents of Bushehr city based on the extended theory of planned behavior. Materials and Methods: In this cross-sectional study, 582 people were selected through convenience sampling method. A questionnaire with items on demographic characteristics, extended theory of planned behavior, and recycling behavior was used for data collection. The collected data were analyzed by SPSS software version 20. Results: In this study, 221 participants (38%) adhered to recycling behavior. There was a significant relationship between knowledge of waste collection program (P<0.001), receiving recycling incentive packages (p=0.003), and recycling waste disposal vehicles (p= 0/001) with recycling behavior. Past behavior (OR=0.229, P<0.001), and descriptive norms (OR=1.187, P=0.003) were predictive of recycling behavior. Conclusion: The results of the present study indicate that regular refuse collection vehicles, providing environmental facilities including incentive packages, and informing citizens about the schedule of refuse collection vehicles can lead to recycling behavior. Also, friends, relatives, and family are role models for other members by establishing norms and are effective in promoting recycling behavior. Having a positive experience of a behavior is necessary for its maintenance.","PeriodicalId":14583,"journal":{"name":"Iranian South Medical Journal","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2020-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43103172","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Qualitaty of Olfaction in Patients with Nasal and Sinuses Polyposis before and after Endo-scopic Sinus Surgery in, Vali e Asr Hospital in 2017 2017年Vali e Asr医院鼻内镜鼻窦手术前后鼻息肉患者的嗅觉质量

Iranian South Medical Journal Pub Date : 2020-06-01 DOI: 10.52547/ismj.23.2.108

M. Asghari, M. Bakhshai

{"title":"Qualitaty of Olfaction in Patients with Nasal and Sinuses Polyposis before and after Endo-scopic Sinus Surgery in, Vali e Asr Hospital in 2017","authors":"M. Asghari, M. Bakhshai","doi":"10.52547/ismj.23.2.108","DOIUrl":"https://doi.org/10.52547/ismj.23.2.108","url":null,"abstract":"Background: Olfaction plays an important role in human life and promotes quality of life through under-standing various odors. Nasal polyps are among the common causes of olfactory impairment. There are many different treatment options for polyps most notably the diagnosis and elimination of underlying causes. Some polyps do not respond to medication therapy or they relapse, and therefore require surgery. The present study aimed to determine olfactory function in patients with sinus and nasal polyposis before and after endoscopy. Materials and Methods: In this cross-sectional study, patients admitted to, Vali e Asr Hospital of Birjand in 2015 for endoscopic nasal polypectomy were included. Evaluation of olfactory quality was performed prior to and four weeks after the surgery. Olfactory function was evaluated by a semi-structured questionnaire on the quality of olfactory, taste and appetite combined with the Quick Odor Detection test. Data was analyzed in SPSS 23 software using descriptive statistics of frequency, perentage, mean and stand-ard deviation, and analytical statistics of McNemar’s and Wilcoxon tests. P value of <0.05 was considered significant. Results: Thirty-six patients were enrolled. All patients underwent endoscopic polypectomy. Their mean age was 39.14±11.69 years. Most of them were male (n=21, 58.3%). History of nasal polyps, asthma, allergic rhinitis and aspirin sensitivity was positive in 27.8%, 11.1%, 47.2% and 27.8% of patients. Nasal polyps were unilateral in 58.3% of patients and bilateral in 41.7% of patients. There was a significant improvement in olfactory quality after surgery (p<0.001). The sense of smell was present in 75% or the patients before and in 100% of them after the surgery, and the difference was statistically significant (p=0.004). Conclusion: Olfactory dysfunction in patients with polyps is remarkably prevalent. Overall, the results of this study show polypectomy significantly improved patients’ sense and quality of smell.","PeriodicalId":14583,"journal":{"name":"Iranian South Medical Journal","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2020-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46560655","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Detection of Heavy Metals Resistance Genes and Effects of Iron Nanoparticles on the Gene Expression in Pseudomonas Aeruginosa Using Real-Time PCR 实时PCR检测铜绿假单胞菌重金属抗性基因及铁纳米粒子对基因表达的影响

Iranian South Medical Journal Pub Date : 2020-03-01 DOI: 10.52547/ismj.23.1.1

Marzeah Rostami, K. Amini, B. Kheirkhah

引用次数: 0

Effective Dose of Radiation in Diagnostic and Interventional Cardiac Angiography among Pediatrics and Adolescents in Hospitals of Shiraz University of Medical Sciences versus Other Radiology Devices 设拉子医科大学附属医院儿科和青少年心脏血管造影诊断和介入的有效辐射剂量与其他放射设备的比较

Iranian South Medical Journal Pub Date : 2020-03-01 DOI: 10.52547/ismj.23.1.27

M. Edraki, E. Karimi, H. Amoozgar, Mohammad Reza Navaeifar

{"title":"Effective Dose of Radiation in Diagnostic and Interventional Cardiac Angiography among Pediatrics and Adolescents in Hospitals of Shiraz University of Medical Sciences versus Other Radiology Devices","authors":"M. Edraki, E. Karimi, H. Amoozgar, Mohammad Reza Navaeifar","doi":"10.52547/ismj.23.1.27","DOIUrl":"https://doi.org/10.52547/ismj.23.1.27","url":null,"abstract":"Background: Cardiac catheterization and angiography are diagnostic and therapeutic imaging modalities that produce the highest X-ray radiation, which might impose lifelong risks to patients. This study aimed to evaluate radiation burden among children and adolescents with congenital heart diseases, who underwent cardiac catheterization and angiography. Then the results were compared with other imaging modalities. Materials and Methods: In this prospective study, children and adolescents with congenital heart diseases were consecutively evaluated from April 2017 till November 2017. This research was designed in Namazi and Faghihi teaching hospitals affiliated to Shiraz University of Medical Sciences, Shiraz, Iran. In addition to the basic data and total radiation time, effective dose and dose area product were assessed for each patient. Results: We enrolled 148 consecutive patients under 18 years old, who underwent 36 diagnostic and 112 interventional cardiac catheterizations. Effective dose was 50.23 mGy in the diagnostic catheterizations, and 48.39 mGy in the interventional angiographies. Also the dose area product was 427/28 mGy.cm 2 in the diagnostic catheterizations, and 476.62 mGy.cm 2 in the interventional angiography. The most time-consuming cine fluoroscopy pertained to ventricular septal defect closure and (11.67 seconds) and the most effective dose and dose area product pertained to the same procedure, as well (68,514 mGy and 623,843 mGy.cm 2 respectively). Conclusion: Effective dose and dose area product in cardiac catheterization were desirable, and some previous studies revealed that carcinogenic effect of X-ray radiation is more prominent when effective dose is more than 70-100 mGy. Thus the effective dose might not cause hazardous outcomes if other x ray modalities are not frequently requested for them in future.","PeriodicalId":14583,"journal":{"name":"Iranian South Medical Journal","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2020-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41598030","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Effectiveness of Treatment Based on Acceptance and Commitment Therapy versus Training Coping Strategies on Anxiety and Despair of Mothers of Children with Autistic Spectrum Disorder 接受和承诺治疗与训练应对策略对自闭症谱系障碍患儿母亲焦虑和绝望的有效性

Iranian South Medical Journal Pub Date : 2020-03-01 DOI: 10.52547/ismj.23.1.56

M. Rostami, Moulod Keykhosrovani, Ali Poladi Rishehri, Mohammadreza Bahrani

{"title":"Effectiveness of Treatment Based on Acceptance and Commitment Therapy versus Training Coping Strategies on Anxiety and Despair of Mothers of Children with Autistic Spectrum Disorder","authors":"M. Rostami, Moulod Keykhosrovani, Ali Poladi Rishehri, Mohammadreza Bahrani","doi":"10.52547/ismj.23.1.56","DOIUrl":"https://doi.org/10.52547/ismj.23.1.56","url":null,"abstract":"Background: The birth of a child with authistic spectrum disorder is a challenge to the family, especially to mothers. The purpose of this research was to compare the effectiveness of acceptance commitment therapy and coping strategies in decreasing anxiety and despair in mothers of children with autism. Materials and Methods: This qausi-experimental multigroup pre-test, post-test design with a control group enrolled 30 mothers of children with autism who were being treated in teaching-treatment centers of Booshehr city using convenience sampling. They were randomly classified into three groups. The experimental groups participated in eight sessions of 90 minutes in 8 weeks, while the control group did not receive any interventions. Beck Anxiety Inventory and Beck Hopelessness Scale were used to collect research data. The collected data were analyzed by multivariate analysis of covariance and Tukey post hoc test. Results: Results showed that acceptance commitment therapy and training coping strategies significantly decreased anxiety and despair in mothers in the experimental groups compared to those in the control group (p<0.01), but the decrease was not significant in the follow-up stage. Tukey's test showed acceptance commitment therapy was significantly more effective for anxiety than training coping strategies in the post-test (p<0.01). Conclusion: Our findings showed that both treatments were effective in decreasing anxiety and despair. Hence, therapists can use these two therapeutic and training approaches, especially acceptance commitment therapy, which is a new wave of cognitive – behavioral therapy.","PeriodicalId":14583,"journal":{"name":"Iranian South Medical Journal","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2020-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49305912","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0