IRAQI JOURNAL OF AGRICULTURAL SCIENCES最新文献

SYNERGISTIC EFFECT OF SPERM FILTRATION TECHNIQUES AND ANTIOXIDANTS ADDED TO EXTENDER OF IRAQI BUFFALO SEMEN 精子过滤技术与添加到伊拉克水牛精液扩展剂中的抗氧化剂的协同效应

IF 0.6

IRAQI JOURNAL OF AGRICULTURAL SCIENCES Pub Date : 2024-02-25 DOI: 10.36103/kc809146

S. N. Alwaeli, S. M. Eidan, Researcher Prof, Depart, Anim, Coll. Agric. Engin. Sci Prod.

{"title":"SYNERGISTIC EFFECT OF SPERM FILTRATION TECHNIQUES AND ANTIOXIDANTS ADDED TO EXTENDER OF IRAQI BUFFALO SEMEN","authors":"S. N. Alwaeli, S. M. Eidan, Researcher Prof, Depart, Anim, Coll. Agric. Engin. Sci Prod.","doi":"10.36103/kc809146","DOIUrl":"https://doi.org/10.36103/kc809146","url":null,"abstract":"This study investigated the synergistic effect of glass wool and sephadex sperm filtration techniques on improving the low semen quality of buffalo bulls with or without antioxidants. Semen was collected for 14 weeks, evaluated weekly, and divided into 12 groups. Good and low semen have extended using a Tris extender. Good semen quality is divided into three groups [S1: Tris extender; S2: Tris + vitamins E (2 mM) and C (5 mM); S3: Tris + glutamine (20 mM) and arginine (1 mM)]. Low semen quality was divided into three main groups and subdivided into three sub-groups (S4; Tris extender; S5: Tris +vitamins E (2 mM) and C (5 mM); S6: Tris + glutamine (20 mM) and arginine (1 mM)). In the 2nd and 3rd main groups, glass wool and sephadex techniques are used with or without adding vitamins (E+C) and amino acids (Glutamine and arginine) and subdivided into three sub-groups with each technique, referred to as S7–S9 for glass wool and S10–S12 for sephadex techniques. Improving (P<0.01) motility, normal morphology, acrosome, and plasma membrane integrity and reducing total sperm abnormalities of low semen quality were noticed using sephadex with or without adding vitamins or amino acids post-cryopreservation. In conclusion, the sephadex technique removed immotile sperm, dead, abnormal, and good sperm harvested by this technique, which in turn may reflect positively on improving the pregnancy rate.","PeriodicalId":14562,"journal":{"name":"IRAQI JOURNAL OF AGRICULTURAL SCIENCES","volume":null,"pages":null},"PeriodicalIF":0.6,"publicationDate":"2024-02-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140432439","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

DETECTION OF GENES RESPONSIBLE FOR HEAVY METALS RESISTANCE IN LOCALLY ISOLATED PSEUDOMONAS SPP. 在本地分离的假单胞菌中检测重金属抗性基因

IF 0.6

IRAQI JOURNAL OF AGRICULTURAL SCIENCES Pub Date : 2024-02-25 DOI: 10.36103/wgz9vb91

Al-Sajad M. S H.A.A. Alsalim, Researcher Prof.

{"title":"DETECTION OF GENES RESPONSIBLE FOR HEAVY METALS RESISTANCE IN LOCALLY ISOLATED PSEUDOMONAS SPP.","authors":"Al-Sajad M. S H.A.A. Alsalim, Researcher Prof.","doi":"10.36103/wgz9vb91","DOIUrl":"https://doi.org/10.36103/wgz9vb91","url":null,"abstract":"Plant growth-promoting rhizobacteria (PGPR) that can tolerate heavy metals, provide the basis for microbial inoculums showing heavy metals tolerance properties. This study was aimed to detect the heavy metal resistance genes in plant-growth-promoting Pseudomonas spp. isolated from many agricultural fields. The collected isolates were screened for their plant growth-promoting (PGP) traits, hydrolytic enzymes, Siderophore, ammonia, and indole-3-acetic acid (IAA). Then, subjected to concentrations of CuSO4, CdCl2, and ZnCl2 to determine the minimum inhibitory concentration (MIC). The DNA was extracted from the selected isolates then PCR test was achieved to detect copA, copB, and czcA genes, responsible for heavy metal resistance. Seventy Pseudomonas spp. isolates were obtained; 41 (58.57%), 6 (8.57%), and 15 (21.42%) isolate produced protease, cellulase, and pectinase, respectively. The isolates were positive for siderophore and ammonia production. However, 68 (97.14%) isolates have produced indole-3-acetic acid. Eight isolates were selected and identified as Pseudomonas aeruginosa using the Vitek 2 compact system. The isolates' resistance to heavy metals differed significantly. The isolate B49 had a higher resistance to CuSO4 (MIC = 3200 µg/ml) and ZnCl2 (MIC = 2600 µg/ml), while the isolate B66 recorded a higher resistance to CdCl2 (MIC = 1000 µg/ml). copB, and czcA genes were detected in the eight P. aeruginosa isolates, while copA gene was detected in seven, except B69.","PeriodicalId":14562,"journal":{"name":"IRAQI JOURNAL OF AGRICULTURAL SCIENCES","volume":null,"pages":null},"PeriodicalIF":0.6,"publicationDate":"2024-02-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140432512","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

ESTIMATION THE EFFECTS OF PLASMA ACTIVATED MEDIUM ON SOME COMPONENTS THAT RELATED WITH GROWING BREAST CANCER CELL LINES 评估血浆活化培养基对乳腺癌细胞株生长相关成分的影响

IF 0.6

IRAQI JOURNAL OF AGRICULTURAL SCIENCES Pub Date : 2024-02-25 DOI: 10.36103/e5wb1502

Rawnaq Z. Fadhil, Mouruj A. Al aubaydi, Kadhim A. Aadim Researcher Prof

{"title":"ESTIMATION THE EFFECTS OF PLASMA ACTIVATED MEDIUM ON SOME COMPONENTS THAT RELATED WITH GROWING BREAST CANCER CELL LINES","authors":"Rawnaq Z. Fadhil, Mouruj A. Al aubaydi, Kadhim A. Aadim Researcher Prof","doi":"10.36103/e5wb1502","DOIUrl":"https://doi.org/10.36103/e5wb1502","url":null,"abstract":"This study was aimed to assess the influence of plasma activated medium (PAM) on some parameters of breast cancer cell lines (MCF7 and AMJ13) in comparison to normal adipose-derived stem cells (ASCs). First, nitric oxide (NO) production within the used media (RPMI-1640 and MEM) was examined beyond different exposure time (10,15,20, and 25) minutes directly, and the finding suggested a significant increase in NO with increasing exposure time particularly after 20 and 25 min of irradiation in RPMI, while MEM showed significant results only after 25 min. The metabolic parameters were studied including glucose uptake and lactate dehydrogenase (LDH) activity. The results found a decrease in LDH activity proportional to incubation times, as over incubation to 48 hrs the activity reduced more significantly than the 24 hours with minimal influence on the enzyme activity in the media. Correlated results were demonstrated in glucose results as it’s concentration was elevated in the media with increasing irradiation doses for all three treated cell lines in compared to untreated homologues.","PeriodicalId":14562,"journal":{"name":"IRAQI JOURNAL OF AGRICULTURAL SCIENCES","volume":null,"pages":null},"PeriodicalIF":0.6,"publicationDate":"2024-02-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140432947","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

EFFECT OF GLYCEROL ON PERFORMANCE AND SOME BLOOD CHARACTERISTICS OF HOLSTEIN CALVES 甘油对荷斯坦犊牛的性能和某些血液特征的影响

IF 0.6

IRAQI JOURNAL OF AGRICULTURAL SCIENCES Pub Date : 2024-02-25 DOI: 10.36103/731zw966

W. A. Khalid, N. N. Al-Anbari, Researcher Prof, Dept, Animal

{"title":"EFFECT OF GLYCEROL ON PERFORMANCE AND SOME BLOOD CHARACTERISTICS OF HOLSTEIN CALVES","authors":"W. A. Khalid, N. N. Al-Anbari, Researcher Prof, Dept, Animal","doi":"10.36103/731zw966","DOIUrl":"https://doi.org/10.36103/731zw966","url":null,"abstract":"This research was to study the effect of adding glycerol at different levels (T1 = control treatment without glycerol, T2 = 75 ml and T3 = 150 ml glycerol) in the body weight, body measurements and some blood characteristics of Holstein calves from 60 to 120 days of calves age. Body weight increased (P≤0.05) in T3 calves at 80 and 100 days of age, also body weight increased in T2 and T3 calves at 120 days of age. T2 calves were significantly superior (P≤0.01) in average total gains from 60 to 120 days of age. Significantly increased (P≤0.05) at the ages of 100 and 120 days of: Wither height in T3 calves, Body length in T2 calves and Heart girth in T2 and T3 calves. Blood glucose concentration increased (P≤0.05) and blood NEFA concentration decreased (P≤0.01) in T2 calves at 80 days of age. At the age of 100 and 120 days, the concentration of blood glucose was increased (P≤0.01 and P≤0.05) and the concentration of NEFA and BHBA decreased (P≤0.01) in calves of the two treatments T2 and T3. We conclude that the addition of glycerol (75 and 150 ml/day) in the feeding of calves increases body weight, promotes growth and improves blood characteristics by increasing blood glucose and reducing NEFA and BHBA concentrations.","PeriodicalId":14562,"journal":{"name":"IRAQI JOURNAL OF AGRICULTURAL SCIENCES","volume":null,"pages":null},"PeriodicalIF":0.6,"publicationDate":"2024-02-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140433025","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

ENHANCE OF FLAVOR AND SOME MICROBIAL PHYSICOCHEMICAL AND SENSORIAL PROPERTIES OF YOGURT BY DRIED CANTALOUPE PULP 用干哈密瓜果肉改善酸奶的风味和某些微生物理化及感官特性

IF 0.6

IRAQI JOURNAL OF AGRICULTURAL SCIENCES Pub Date : 2024-02-25 DOI: 10.36103/f4pyxy69

Al-Zobaay W. J.M, Al-Zobaay A.H.H, Researcher Assist. Prof. Dept. of Food Sci

{"title":"ENHANCE OF FLAVOR AND SOME MICROBIAL PHYSICOCHEMICAL AND SENSORIAL PROPERTIES OF YOGURT BY DRIED CANTALOUPE PULP","authors":"Al-Zobaay W. J.M, Al-Zobaay A.H.H, Researcher Assist. Prof. Dept. of Food Sci","doi":"10.36103/f4pyxy69","DOIUrl":"https://doi.org/10.36103/f4pyxy69","url":null,"abstract":"This study was aimed to enhance the flavor compounds, improve sensorial, microbial and physicochemical characteristics of the yogurt product using dried cantaloupe pulp. This study included the estimation of three flavor compounds in the yogurt product (acetaldehyde, acetone, and ethanol), after seven day of refrigeraed storage at 4°C, a wide difference in the levels of flavor compounds among the treatments have been shown, the values of acetaldehyde were in the following treatments (A) control treatment, (H) cantaloupe 1 %, (K) cantaloupe3%, as follows 13.46, 14.27, 24.54 mg/L respectively. Acetone values were 8.38, 5.33, 3.47 mg/L respectively. Ethanol were 10.25, 7.12, 4.29 mg/L respectively. Lactobacillus delbrueckii subsp. Bulgaricus log vaible count after the first day of manufacture were 7.38, 8.53, 8.77 cfu ml-1 for A, H and K treatment respectively, gradually decreased through refrigerated storage to reach after 28 days 6.25, 7.41, 7.60 cfu ml-1 respectively. The log vaible count Streptococcus silvarius subsp. Thermophilus at first day of manufacture were 8.20, 9.66, 9.71 cfu ml-1 for A, H and k treatment respectively, gradually decreased under refrigerated storage to 7.25, 8.50, 8.30 cfu ml-1 respectively after 28 days. Chemical composition did not change, and there were no significant differences among the treatments. Sensory evaluation values showed differences in treatment, the flavor scores for treatments after 28 days of refrigerated storage were (37, 42, 43), the texture were (25, 28, 29), the appearance were (10,14, 14), color (9, 8, 8) for A, H and k treatment respectively. The treatment K was superior as compared to the other treatments with a score of acceptance (94), compared with the control treatment whose score of acceptance was(81) out to 100","PeriodicalId":14562,"journal":{"name":"IRAQI JOURNAL OF AGRICULTURAL SCIENCES","volume":null,"pages":null},"PeriodicalIF":0.6,"publicationDate":"2024-02-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140433067","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

INFLUENCE OF SEX ON GROWTH PERFORMANCE, CARCASS ATTRIBUTES, MEAT QUALITY, AND BLOOD METABOLITES OF AWASSI LAMBS 性别对阿瓦西羔羊生长性能、胴体属性、肉质和血液代谢物的影响

IF 0.6

IRAQI JOURNAL OF AGRICULTURAL SCIENCES Pub Date : 2024-02-25 DOI: 10.36103/tryb9y64

Ja’far Al-Khaza’leh, B. Obeidat

引用次数: 0

EVALUATION OF THE SMALL FARMERS FUND IN THE AGRICULTURAL INITIATIVE IN IRAQ THROUGH THE COLLECTION EFFICIENCY OF LOANS FOR THE PERIOD 2009 – 2018 通过 2009-2018 年期间贷款的回收效率对伊拉克农业倡议中的小农基金进行评估

IF 0.6

IRAQI JOURNAL OF AGRICULTURAL SCIENCES Pub Date : 2024-02-25 DOI: 10.36103/8508sa16

Sanaa J. Mohammed, M. H. O. A. Khazeli, B. H. Al-Badri

{"title":"EVALUATION OF THE SMALL FARMERS FUND IN THE AGRICULTURAL INITIATIVE IN IRAQ THROUGH THE COLLECTION EFFICIENCY OF LOANS FOR THE PERIOD 2009 – 2018","authors":"Sanaa J. Mohammed, M. H. O. A. Khazeli, B. H. Al-Badri","doi":"10.36103/8508sa16","DOIUrl":"https://doi.org/10.36103/8508sa16","url":null,"abstract":"To evaluate and analyze the small farmers fund, one of the funds financed by the allocation of the Supreme Committee for the Agricultural Initiative, this fund finance the unconditioned plastic houses, beekeeping, field crops, winter and summer vegetable crops, protected tomato houses, olive trees services, planting of green silage by fixed sprinkler irrigation and to construct consulting offices. As the total number of loans granted to this fund reached nearly 481 billion dinars in all governorates, amounting to 38991 beneficiaries, constituting about 23% of the total loans disbursed during the study period, Baghdad governorate has 68.6 billion of total amounts of that fund and that consist 16.6% of it, then Anbar came in the second rank by 14.7% then Wasit came in the third rank by 12%, while Kirkuk came in the last. The study reached an analysis of the achievement ratios of the small farmers fund for the period 2009-2018 for all governorates, the provinces of Baghdad and Basra were highest share of this fund for the year 2009, while the lowest share for these year were for Mysan and Kirkuk. In 2010, Anbar has the highest share while Kirkuk was the lowest. In 2011-2012, the governorate of Baghdad ranked first, and the lowest were for Kirkuk governorate, in 2013, Anbar came to the first, the lowest was Kirkuk governorate, then Wasit governorate returned to first place with collection rates for 2014 and in 2015 Najaf was the first and for 2016 and 2017, the governorate of Baghdad came first. There is a clear decrease in the rates of late loan repayment in this fund because these loans are closely related to the guarantees that document the loans. Most of the guarantees of these loans are the bill only, whereby the amount of withdrawals loans of all kinds is proportional to the amount of the guarantee on the bill of exchange 40 million dinars. The study recommended the necessity of conducting a field survey of all the purposes that the small farmers fund.","PeriodicalId":14562,"journal":{"name":"IRAQI JOURNAL OF AGRICULTURAL SCIENCES","volume":null,"pages":null},"PeriodicalIF":0.6,"publicationDate":"2024-02-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140433278","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

PHYTOCHEMICAL DIVERSITY AND NUTRITIONAL VALUE OF KENGER AT DIFFERENT LOCATIONS IN SULAIMANI REGION - IRAQ 伊拉克苏莱曼尼地区不同地点 Kenger 的植物化学多样性和营养价值

IF 0.6

IRAQI JOURNAL OF AGRICULTURAL SCIENCES Pub Date : 2024-02-25 DOI: 10.36103/t7rmmq75

Rozhgar. M. Ahmed

{"title":"PHYTOCHEMICAL DIVERSITY AND NUTRITIONAL VALUE OF KENGER AT DIFFERENT LOCATIONS IN SULAIMANI REGION - IRAQ","authors":"Rozhgar. M. Ahmed","doi":"10.36103/t7rmmq75","DOIUrl":"https://doi.org/10.36103/t7rmmq75","url":null,"abstract":"Kenger (Gundelia tournefortii L.) plants were collected based on the availability of these plants in the local market to determine the nutrition value, some phytochemical content, and element content during March 2023 at the vegetative stage at thirteen different locations in the Sulaimani region. Some vegetative traits and chemical contents were studied. The results indicated that the highest content of carbohydrates was (12.670%) at Mawat, the protein was (32.703%) at Hazarmerd, and fiber was (35.620%) at Penjwen locations respectively. The analysis of Kenger edible parts (stem and leaves) through GC-MS revealed the presence of a complex mixture of compounds varying from 13 to 19 compounds among the locations. Compounds such as carboxylic acids ranged from 25.217% at Penjwen to 77.043% at Temar, terpenes ranged from 15.912% in Temar to 57.749% in Penjwen, and sterols ranged from 5.351% at Qara Dagh to 24.688% at Hazarmerd. In other locations, these compounds were detected in between those ranges, with differences in their concentrations. According to the results obtained from ICP-OES analysis, macro-elements (Ca, K, P, S, Mg, and Na) contents between the studied locations, the highest concentration of 3.127% occurred at Temar location, the lowest value was obtained at Gulp location with 2.468%, with an average of 2.811% for all locations.","PeriodicalId":14562,"journal":{"name":"IRAQI JOURNAL OF AGRICULTURAL SCIENCES","volume":null,"pages":null},"PeriodicalIF":0.6,"publicationDate":"2024-02-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140432611","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

ISOLATION AND CHARACTERIZATION OF LISTERIA MONOCYTOGENES FROM SOME IRAQI MISCARRIAGE WOMEN 从一些伊拉克流产妇女体内分离出李斯特菌并确定其特征

IF 0.6

IRAQI JOURNAL OF AGRICULTURAL SCIENCES Pub Date : 2024-02-25 DOI: 10.36103/cvh62311

K. K. Ghaima, D. S. Qader, Prof Assist, Instit. of, Biotech. for, Postgrad. Stud

{"title":"ISOLATION AND CHARACTERIZATION OF LISTERIA MONOCYTOGENES FROM SOME IRAQI MISCARRIAGE WOMEN","authors":"K. K. Ghaima, D. S. Qader, Prof Assist, Instit. of, Biotech. for, Postgrad. Stud","doi":"10.36103/cvh62311","DOIUrl":"https://doi.org/10.36103/cvh62311","url":null,"abstract":"This study was aimed to find out how common Listeria monocytogenes in a group of Iraqi women who had miscarriages. Also, investigates the dependability of molecular diagnosis, specifically evaluating the target hlyA gene as an alternate solution tool for identification. A total of 200 specimens comprising cervical swabs were collected from 176 women suffered from miscarriage and 24 women as control. The detection of L. monocytogenes in miscarriages women by using HiCrome™ Listeria Agar Base as both a selective and differential agar medium for quick and direct recognition of Listeria species. The biochemical tests were achieved for confirmation identification. In addition, virulence-associated genes (hlyA gene) isolates were identified using a Real-time PCR approach. Results show out of the 200 (patients and control) specimens, 176 women with miscarriages were analyzed and it was found that 117 (66.5%) specimens from abortion cases were negative for L. monocytogenes, while 59 (33.5%) specimens were positive for Listeria species. All 24 women of control give negative result. Seventy-five (32.4%) isolates from the 59 positive women showed the presence of the hlyA gene in Real-time PCR. We found that molecular diagnostic through Real-Time PCR is a quick and accurate diagnostic method that can help minimize the time it takes to start a specific treatment and track its success. ","PeriodicalId":14562,"journal":{"name":"IRAQI JOURNAL OF AGRICULTURAL SCIENCES","volume":null,"pages":null},"PeriodicalIF":0.6,"publicationDate":"2024-02-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140432970","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

MOLECULAR CHARACTERIZATION OF M. PHASEOLINA AND ITS MANAGEMENT USING AGROCHEMICALS AND T. HARZIANUM M.Phaseolina的分子特征及其利用农用化学品和T.

IF 0.6

IRAQI JOURNAL OF AGRICULTURAL SCIENCES Pub Date : 2024-02-25 DOI: 10.36103/4p59fb72

K. A. Sido, W. A. Hassan

{"title":"MOLECULAR CHARACTERIZATION OF M. PHASEOLINA AND ITS MANAGEMENT USING AGROCHEMICALS AND T. HARZIANUM","authors":"K. A. Sido, W. A. Hassan","doi":"10.36103/4p59fb72","DOIUrl":"https://doi.org/10.36103/4p59fb72","url":null,"abstract":"Systemic Acquired Resistance (SAR) induced by agrochemicals of chitosan (CH) and salicylic acid (SA) at (25, 50 and 100 ppm) , in addition to a biocontrol agent of T. harzianum (Th) at 4 ×106 were examined against M. phaseolina the causal agent of charcoal rot of sunflower. The results depended on estimation of diseases severity and microsclerotia density in the soil. Thus, the seeds immersion in CH 75 ppm for 6 h., gave the highest and considerable reduction (p=0.05) in disease severity by 48.25% and reduced microsclerotia survived in the soil up to 70%. Application of SA at 50 and 75 ppm proved an obvious reduction of charcoal rot severity by up to 39% and 37% for both concentrations, respectively and not varied with Th. The results also confirmed that CH at 75 ppm revealed significant reduction 40.63% in disease severity and similarized with SA at same concentration. However, the lowest dose of SA at 25 ppm realized the highest reduction of micro sclerotia density by 80.28 % compared to 74.91% when used CH at 75 ppm. For molecular identification of a pathogen Polymerase Chain Reaction (PCR) using ITS4 and ITS5 universal primers were applied to amplify and sequence of DNA for six isolates of M. phaseolina viz., OL901219, OL636051, OL901220, OL901204, OL636050 and OL636053 compared for identity of rDNA sequence according to NCBI GenBank databases by BLAST mode and the results showed the entire similarity ratio reached to 100%","PeriodicalId":14562,"journal":{"name":"IRAQI JOURNAL OF AGRICULTURAL SCIENCES","volume":null,"pages":null},"PeriodicalIF":0.6,"publicationDate":"2024-02-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140433189","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0