EMERGENCE OF MULTIDRUG RESISTANT BACTERIA AMONG PATIENTS WITH RESPIRATORY TRACT INFECTIONS

IF 1.1 Q3 AGRICULTURE, MULTIDISCIPLINARY

IRAQI JOURNAL OF AGRICULTURAL SCIENCES Pub Date : 2023-12-29 DOI:10.36103/ijas.v54i6.1860

Omaima S Al-Qaissy, A. S. Al-Khafaji

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引用次数: 0

Abstract

In this study, 25 Pseudomonas aeruginosa isolates were diagnosed in 160 clinical samples collected from patients attending the Tuberculosis Institute/ Medical city and Al-Muhmodia Public Hospital, in addition to a number of private laboratories in Baghdad during the period from September 2020 to February 2021. The bacterial isolates from clinical samples were biochemically diagnosed by API® 20E system then further identified by VITEK 2 system. P. aeruginosa represented the most prevalent bacteria in lower respiratory tract specimens. These isolates showed moderate to high susceptibility towards 11 antimicrobial agents tested in this study except that of ceftazidime and ceftriaxone. Extra-chromosomal resistance of the top three of the most resistant P. aeruginosa isolates were subjected for molecular investigation. Bacterial transformation of E. coli S1 cells with plasmid DNA extracted from the selected P. aeruginosa isolates exhibited increased resistance to ceftazidime and penicillin G. The data clearly suggest that the plasmid DNA content is implicated in enhancement of P. aeruginosa resistance through horizontal transfer of extra- chromosomal beta lactam resistance associated genes.

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呼吸道感染患者中出现耐多药细菌

本研究在 2020 年 9 月至 2021 年 2 月期间从巴格达结核病研究所/医疗城和 Al-Muhmodia 公立医院以及一些私人实验室收集的 160 份临床样本中诊断出 25 个铜绿假单胞菌分离物。从临床样本中分离出的细菌通过 API® 20E 系统进行生化诊断，然后通过 VITEK 2 系统进行进一步鉴定。铜绿假单胞菌是下呼吸道标本中最常见的细菌。除头孢他啶和头孢曲松外，这些分离菌株对本研究中测试的 11 种抗菌药物具有中度至高度敏感性。对耐药性最强的前三种铜绿假单胞菌分离株的染色体外耐药性进行了分子研究。用从所选铜绿假单胞菌分离物中提取的质粒 DNA 对大肠杆菌 S1 细胞进行细菌转化，结果显示其对头孢他啶和青霉素 G 的耐药性增强。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

IRAQI JOURNAL OF AGRICULTURAL SCIENCES AGRICULTURE, MULTIDISCIPLINARY-

CiteScore

2.30

自引率

50.00%

发文量

140

审稿时长

6 weeks

期刊介绍： IRAQI JOURNAL OF AGRICULTURAL SCIENCES (IJAS)is the first agric. scientific and refereed journal established in Iraq. The first volume was published in 1966. IJAS is registered in the number 137 in 1988 of the Baghdad National Library. Years ago, it was published with one issue a year. For the time being, it is published bimonthly (6 issues for a volume). IJAS Deal with: Field Crops. Plant Breeding. Agricultural Economics. Agricultural Extension. Agricultural Mechanization. Basic sciences. Hort. Sciences. Animal Husbandry. Food Technology, Plant Pathology. Plant Entomology. Poultry Sciences. Soil Sciences. Water Resources. Veterinary. Biology.