Cell Biology and Development最新文献

{"title":"Effect of antibiotics in eliminating bacterial wilt (Ralstonia solanacearum) from in vitro propagated ginger","authors":"T. Markos","doi":"10.13057/cellbioldev/v040105","DOIUrl":"https://doi.org/10.13057/cellbioldev/v040105","url":null,"abstract":"Abstract. Markos T, Feyissa T. 2020. Effect of antibiotics in eliminating bacterial wilt (Ralstonia solanacearum) from in vitro propagated ginger. Cell Biol Dev 4: 46-52. Ginger (Zingiber officinale Roscoe) is a herbaceous perennial. It is cultivated commercially in most tropical regions of the world and is a member of the Zingiberaceae family. In conventional ginger cultivation, underground rhizomes are used as planting material. However, it is afflicted by diseases that result in the senescence and degeneration of tissues. Due to Ralstonia solanacearum infection, substantial rhizome losses have been recorded. Therefore, disease-free planting material is required to cultivate ginger successfully. Plant tissue culture technology has been successfully used to commercialize pathogen-free plants and conserves the germplasm of rare and endangered species. In in-vitro, culture techniques offer an alternative method of plant multiplication and a method for crop enhancement. Frequently, ginger multiplication media are contaminated with R. solanacearum, which can survive endophytically in plantlets, rendering them useless for in vitro propagation. Therefore, an experiment was undertaken to determine the efficiency of antibiotics against the in vitro development of R. solanacearum. Four antibiotics, gentamicin, tetracycline, ampicillin, and streptomycin, at four concentrations, 130 mg/L, 160 mg/L, 200 mg/L, and 250 mg/L, were evaluated for their ability to eradicate bacteria from in-vitro propagated ginger. Gentamicin was proven superior to other therapies, with the maximum inhibition (22 mm) at 250 mg/L, followed by tetracycline at 200 mg/L (18 mm), streptomycin at 130 mg/L (13 mm), and ampicillin at 130 mg/L (12 mm). The R. solanacearum can be eradicated from in vitro propagated ginger by applying antibiotics in the micropropagation media.","PeriodicalId":145551,"journal":{"name":"Cell Biology and Development","volume":"27 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2019-01-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"130473319","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Morphology and cytology of five soybean varieties (Glycine max) treated with phosphate fertilizer","authors":"Damar Cipto Darsono, N. Nandariyah, Sugijono Sugijono","doi":"10.13057/cellbioldev/v020205","DOIUrl":"https://doi.org/10.13057/cellbioldev/v020205","url":null,"abstract":"Abstract. Darsono DC, Nandariyah, Sugijono. 2018. Morphology and cytology of five soybean varieties (Glycine max) treated with phosphate fertilizer. Cell Biol Dev 2: 78-87. The demand for soybeans (Glycine max (L.) Merrill) tends to increase. The first step to increasing soybean productivity is identifying the plant's character. This study aims to study plants' morphological and cytological characteristics (chromosome number) and determine the effect of phosphate fertilizer application on changes in plant morphological and cytological characteristics (chromosome number) in five soybean varieties. The research was carried out from April 2009 to March 2010. Observations of plant morphology were carried out at the Jumantono Dry Land Research and Development Center (Pusat Penelitian dan Pengembangan Lahan Kering Jumantono), Karanganyar, Central Java, Indonesia (07°37' latitude and 110°56' east longitude). The number of chromosomes was analyzed in the Laboratory of Plant Breeding, Faculty of Agriculture, Universitas Sebelas Maret, Surakarta, Central Java, Indonesia, and the Laboratory of Animal Anatomy, Faculty of Biology, Universitas Gadjah Mada, Yogyakarta, Indonesia. Plant morphology research was arranged factorially using a Completely Randomized Block Design (RAKL). The treatment factors were varieties (Argomulyo, Anjasmoro, Kaba, Sibayak, and Wilis) and doses of P fertilizer (0, 18, and 36 kg P2O5/ha (equivalent to 0, 50, and 100 kg SP-36/ha). Therefore, there were 15 treatment combinations, and each combination was repeated 3 times. The observation of the number of chromosomes used the squash method. Quantitative data were analyzed by Analysis of Variance (ANOVA), if significantly different, continued with Duncan's multiple distance test (DMRT) level of 5%. The qualitative analysis was analyzed and presented descriptively. The results showed that each of the five soybean varieties' morphological characteristics differed. Applying phosphate (P) fertilizer from a dose of 0, 18, or 36 kg P2O5/ha could affect plant morphology in the form of an increase in plant height, length of the main root, number of branch roots, length of stem internode, length of petiole, leaf area, and weight of 100 seeds in each soybean variety. The number of chromosomes in five soybean varieties is the same, namely 2n = 40. The application of phosphate (P) fertilizer did not affect the number of chromosomes in the five soybean varieties.","PeriodicalId":145551,"journal":{"name":"Cell Biology and Development","volume":"22 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2018-12-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"124484478","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Date palm (Phoenix dactylifera) seeds germination","authors":"N. Mohammed, A. G. E. Said","doi":"10.13057/cellbioldev/v020203","DOIUrl":"https://doi.org/10.13057/cellbioldev/v020203","url":null,"abstract":"Abstract. Mohammed NMI, Said AGE. 2018. Date palm (Phoenix dactylifera) seeds germination. Cell Biol Dev 2: 63-68. This study aimed to examine if pre-germination treatments could effectively promote date palm (Phoenix dactylifera L.) seed germination in the Nursery of the Horticultural Sector, Ministry of Agriculture and Irrigation, Khartoum, Sudan. Seeds were soaked in water for 0, 2, 4, 6, 8, 10, and 12 days before being planted in one of the following media: sand:clay (1:1), sand:clay (1:2), sand:clay (1:0), sand:clay (0:1), and sand:clay (2:1). The factorial completely randomized block design was employed in this investigation. The 6-day soaking time resulted in the highest percentage of seeds germinating; as the soaking time increased, the percentage of seeds germinating decreased. The 6-day soaking time also improved seed germination, allowing for a 50 % final germination percentage in the shortest time. Sowing seeds in a 2:1 soil mix decreased germination days, resulting in a substantial increase in germination percentage compared to other soil type mixes evaluated, regardless of soaking duration. In addition, seeds soaked for 6 days and planted in a 2:1 soil mix produced the tallest seedlings.","PeriodicalId":145551,"journal":{"name":"Cell Biology and Development","volume":"104 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2018-12-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"126569873","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The effect of sucrose concentration on growth and induction of somatic embryogenesis in Catharanthus roseus callus","authors":"David Cahyo Herwinaldo, Solichatun Solichatun, Endang Anggarwulan","doi":"10.13057/cellbioldev/v020204","DOIUrl":"https://doi.org/10.13057/cellbioldev/v020204","url":null,"abstract":"Abstract. Mohammed NMI, Said AGE. 2018. Date palm (Phoenix dactylifera) seeds germination. Cell Biol Dev 2: 63-68. This study aimed to examine if pre-germination treatments could effectively promote date palm (Phoenix dactylifera L.) seed germination in the Nursery of the Horticultural Sector, Ministry of Agriculture and Irrigation, Khartoum, Sudan. Seeds were soaked in water for 0, 2, 4, 6, 8, 10, and 12 days before being planted in one of the following media: sand:clay (1:1), sand:clay (1:2), sand:clay (1:0), sand:clay (0:1), and sand:clay (2:1). The factorial completely randomized block design was employed in this investigation. The 6-day soaking time resulted in the highest percentage of seeds germinating; as the soaking time increased, the percentage of seeds germinating decreased. The 6-day soaking time also improved seed germination, allowing for a 50 % final germination percentage in the shortest time. Sowing seeds in a 2:1 soil mix decreased germination days, resulting in a substantial increase in germination percentage compared to other soil type mixes evaluated, regardless of soaking duration. In addition, seeds soaked for 6 days and planted in a 2:1 soil mix produced the tallest seedlings.","PeriodicalId":145551,"journal":{"name":"Cell Biology and Development","volume":"64 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2018-12-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"131812254","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Production of potato (Solanum tuberosum) pre-basic seed through tissue culture in Katibougou, Mali","authors":"M. Abdoulaye","doi":"10.13057/cellbioldev/v050205","DOIUrl":"https://doi.org/10.13057/cellbioldev/v050205","url":null,"abstract":"Abstract. Abdoulaye M, Blay ET, Eleblu JSY. 2021. Production of potato (Solanum tuberosum) pre-basic seed through tissue culture in Katibougou, Mali. Cell Biol Dev 5: 90-104. In Mali, the main problem limiting the productivity and production of potatoes is the non-availability of quality seeds in adequate quantities and at affordable prices. This study proposes two experiments on the techniques adopted in Mali's IPR/IFRA plant biotechnology laboratory to meet quantity and quality improvements. That evaluation of the effects of 3 concentrations of coconut water and 2 of potassium nitrate on potato plantlets growth in vitro and of 2 physiological ages and 3 substrates on potato in vitro plants' establishment, post in vitro growth, and mini tubers production in vivo. The first experiment was a Completely Randomized Design (CRD) with 12 treatments replicated 4 times. The second was a factorial experiment with 2 factors (physiological ages: 2 levels and substrate: 3 levels) in a Randomized Complete Block Design (RCBD) with 6 treatments replicated 4 times. The first experiment showed that the culture medium M7 (MS+40 mL/L of coconut water +250 mg of potassium nitrate) had promoted all plant growth parameters (shoot emergence, plant height, number of nodes, leaves and roots, and plant fresh and dry weight) after 30 days of in vitro culturing. The lower concentrations of coconut water (40 mL) and potassium nitrate (250 mg) per liter of MS medium had significant and positive effects on all the in vitro growth parameters after 30 days. The second experiment showed that the plantlet weaning age of 25 days and the post-flask culture substrate S1 (only soil) provided the best plant survival percentage at 20 days after transplanting in vivo. The substrate S2 (soil and cow dung 2:1) positively affects plant stem length, stem diameter, fresh and dry biomass formation, tuber yield, tuber numbers per plant, and tuber grading size B (tubers with a diameter of less than 28 mm). The substrate composition S1 (only soil) has significantly affected the weight loss (12.50%) of tubers stored within 8 weeks. The weaning age was 45 days, and the substrates S2 (soil and cow dung 2:1) and S3 (soil and cow dung 1:1) significantly reduced the number of sprouts per tuber and sprouts number per eye on tubers. In addition, the results indicate that for the better and more rapid growth of potato plantlets in vitro culture, the coconut water concentration used as a supplement to MS medium should be 40 mL per liter of medium. The proportion of cow dung used in substrate composition should not exceed the soil and cow dung ratio 2:1 for maximum post-transplanting plant re-establishment rate in vivo and rapid maturity of mini-tubers.","PeriodicalId":145551,"journal":{"name":"Cell Biology and Development","volume":"2006 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2018-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"127665377","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The effect of adding coconut pulp resulting from Aspergillus oryzae fermentation in commercial feed on the growth of Nile tilapia (Oreochromis niloticus)","authors":"Puri Elyana, A. Pangastuti, E. R. Nugraheni","doi":"10.13057/cellbioldev/v020105","DOIUrl":"https://doi.org/10.13057/cellbioldev/v020105","url":null,"abstract":"Abstract. Elyana P, Pangastuti A, Nugraheni ER. 2018. The effect of adding coconut pulp resulting from Aspergillus oryzae fermentation in commercial feed on the growth of Nile tilapia (Oreochromis niloticus). Cell Biol Dev 2: 33-42. Nile tilapia (Oreochromis niloticus Linnaeus, 1758) is one type of fish with the potential as an animal protein source. Quality feed, particularly feed containing basic protein nutrients, is required to increase fishery product production. Coconut pulp is one type of household waste with a relatively high nutritional content, particularly protein, and the potential to be processed into fish feed ingredients. Coconut pulp is initially processed through a fermentation process with Aspergillus oryzae (Ahlb.) Cohn, which is expected to increase protein digestibility. This study aims to determine the effect of adding fermented coconut pulp as a mixture in feed on the protein content and growth of Nile tilapia. This study used an experimental method called Completely Randomized Design (CRD). This study lasted 60 days and included four treatments containing fermented coconut pulp and commercial pellets. The composition of each treatment I, II, III, and IV was 75%:25%; 50%:50%; 25%:75%; and 0%:100%. Each treatment was replicated three times. Data analysis revealed that adding 75% fermented coconut pulp to the feed increased the water, fat, and crude fiber content by 25.72%, 20.36%, and 10.56%, respectively. In addition, the growth of Nile tilapia increased after they were fed fermented coconut pulp. The concentration of adding coconut pulp to the optimal feed for Nile tilapia growth and protein content was 25%. However, it is necessary to improve the nutritional composition of the feed to increase growth and meat protein.","PeriodicalId":145551,"journal":{"name":"Cell Biology and Development","volume":"13 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2018-06-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"127804740","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effect of cytokinin and gibberellic acid applications on seed germination and growth of Rauvolfia verticillata plant","authors":"S. Lestari, Solichatun Solichatun, Endang Anggarwulan","doi":"10.13057/cellbioldev/v020104","DOIUrl":"https://doi.org/10.13057/cellbioldev/v020104","url":null,"abstract":"Abstract. Lestari S, Solichatun, Anggarwulan E. 2018. Effect of cytokinin and gibberellic acid applications on seed germination and growth of Rauvolfia verticillata plant. Cell Biol Dev 2: 27-32. The purposes of this research are to study the effect of cytokinin and gibberellic acid (GA3) on seed germination and growth of Rauvolfia verticillata Lour. or pule pandak and to determine the best treatment combination that has an optimal effect on the seed germination and growth of R. verticillata. This study was done in a complete randomized design by combination treatment of gibberellic acid and cytokinin that consist of 6 treatment combinations including GA3 0 ppm/ cytokinin 0 ppm, GA3 50 ppm/ cytokinin 0 ppm, GA3 0 ppm/ cytokinin 50 ppm, GA3 50 ppm/ cytokinin 50 ppm, GA3 50 ppm/ cytokinin 75 ppm, GA3 75 ppm/ cytokinin 50 ppm and 8 replicates. The treatments were given by soaking the mature seed in the hormone solution. Some parameters such as germination and growth parameter were measured. The result shows that presoaking treatment with GA3 50 ppm had a greater influence on germinating time, germination percentage, plant height, leaf number, leaf wide, and dry weight than presoaking use cytokinin 50 ppm. Presoaking treatment using a combination of GA3 and cytokinin (G50S50) has a significant influence on increasing fresh weight and dry weight. The combination of GA3 and cytokinin in the concentration of G50S75 influences fresh weight and dry weight plants. The combination of GA3 and cytokinin in concentration G75S50 influences increased germination percentage, plant height, leaf number, and leaf wide.","PeriodicalId":145551,"journal":{"name":"Cell Biology and Development","volume":"5 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2018-05-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"127639960","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effect of organic and inorganic fertilizers on growth, yield and nutrient use efficiency of clonal tea (Camellia sinensis)","authors":"Bitange N. Mokaya, G. Chemining’wa, J. Ambuko, R. Nyankanga","doi":"10.13057/cellbioldev/v020103","DOIUrl":"https://doi.org/10.13057/cellbioldev/v020103","url":null,"abstract":"Abstract. Mokaya BN, Chemining’wa GN, Ambuko JL, Nyankanga RO. 2018. Effect of organic and inorganic fertilizers on growth yield and nutrient use efficiency of clonal tea (Camellia sinensis). Cell Biol Dev 2: 15-26. This research observed how to boost tea yields in small-scale tea producers using inorganic NPK fertilizers yearly while the cost of fertilizers has been rising, resulting in a decrease in net returns. The influence of different rates of organic and inorganic fertilizers on the soil chemical characteristics, growth, yield, and nutrient usage efficiency of clonal tea was investigated in an experiment. In the 2014/2015 production year, experiments were put up in Kianjokoma, Embu County. Organic Rutuba® and inorganic NPK fertilizers were employed (26.5.5). No-fertilizer control, 625 kg NPK/ha, 937.5 kg NPK/ha, 1875 kg NPK/ha, 625 kg Rutuba/ha + 625 kg NPK/ha, 625 kg Rutuba/ha, 937.5 kg Rutuba/ha, and 1875 kg Rutuba/ha were the treatments. These treatments were repeated three times in a randomized full-block design. Green leaf yield, black made tea yield, leaf length, and leaf breadth were among the crop statistics obtained. The soil data collected at the trial included the pH, exchangeable acidity, organic carbon, micronutrients, and macronutrients. Plant utilization efficiency for nitrogen, phosphate, and potassium were also measured. The cost and net revenue of each fertilizer treatment were calculated as well. The data were subjected to analysis of variance, and the least significant difference test was used to separate the groups at p 0.05. Adding organic Rutuba to the soil raised the pH and the amounts of Mn, Cu, Fe, Zn, Ca, and Mg. Compared to the no-fertilizer control and farmers' practice, the application of 625 kg Rutuba/ha + 625 kg NPK/ha resulted in enhanced leaf length, breadth, fresh green leaf yield, and made tea yield. According to the findings, the application of Rutuba fertilizer considerably improved agronomic efficiency, apparent nutrient recovery, and partial factor productivity of N, P, and K compared to inorganic NPK fertilizer. The study also found that applying NPK 26.5.5 at rates more than 625 kg/ha did not affect growth, yield, nutrient usage efficiency, or net returns. However, because organic Rutuba has a low macronutrient level, the study indicated that it should not be used alone in tea manufacturing. More research is needed to discover the best NPK and Rutuba fertilizer mix, as well as the influence of organic Rutuba fertilizer on tea quality.","PeriodicalId":145551,"journal":{"name":"Cell Biology and Development","volume":"53 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2018-03-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"115652866","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Morphological and cytological analysis of yellow skin dragon fruit (Selenicereus megalanthus)","authors":"A. Setyowati, S. Sukaya, E. Yuniastuti","doi":"10.13057/cellbioldev/v020102","DOIUrl":"https://doi.org/10.13057/cellbioldev/v020102","url":null,"abstract":"Abstract. Setyowati A, Sukaya, Yuniastuti E. 2018. Morphological and cytological analysis of yellow skin dragon fruit (Selenicereus megalanthus). Cell Biol Dev 2: 8-14. Dragon fruit is a tropical fruit that has the potential to be developed. Information on morphological and cytological characters of dragon fruit plants is still small and simple, so morphological and cytological analysis is necessary. The morphological and cytological analysis yielded useful information to support the dragon fruit plant breeding program. This study aims to study the morphological and cytological characteristics (karyotype) of the yellow skin dragon fruit (Selenicereus megalanthus (Schum. ex Vaupel) Moran). This study took samples from \"Kusumo Wanadri Agrotourism\" Glagah Indah Beach, Yogyakarta, Indonesia. Chromosomal identification was carried out at the Plant Breeding Laboratory, Faculty of Agriculture, Universitas Sebelas Maret, Indonesia. The research was carried out from December 2006 to June 2008. The methods used were field observations and treatment methods in water for 24 hours at a temperature of 5-8ºC, fixation in 45% acetic acid solution for 2 hours at a temperature of 5-8ºC, and hydrolysis in an aqueous solution. One (1) N HCl for 3-4 minutes at 60ºC, staining in 2% aceto-orcein solution for 24 hours at 5-8ºC and squashing. Morphological and cytological data were analyzed and presented descriptively. The morphology of the plant S. megalanthus is a white thread-shaped root with a fibrous plant root system. The stem is a smooth, faceted wet stem with concave edges. The fruit is oval, surrounded by short spines, the skin is yellow, and the flesh is white. The S. megalanthus has a tetraploid chromosome 2n = 4X = 44 with a chromosome length ranging from 2 + 0.098 m to 4.75 + 0.98 m. The karyotype formula for the S. megalanthus chromosome 2n = 4X = 44 = 40 m + 4 sm.","PeriodicalId":145551,"journal":{"name":"Cell Biology and Development","volume":"22 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2018-03-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"124639172","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effect of type of seedling media and duration of synthetic auxin immersion on germination and initial growth of papaya (Carica papaya) seedlings","authors":"A. Mulati, S. Supriyono, Warsoko Wiryowidodo","doi":"10.13057/cellbioldev/v010205","DOIUrl":"https://doi.org/10.13057/cellbioldev/v010205","url":null,"abstract":"Abstract. Mulati A, Supriyono, Wiryowidodo W. 2017. Effect of type of seedling media and duration of synthetic auxin immersion on germination and initial growth of papaya (Carica papaya) seedlings. Cell Biol Dev 1: 64-70. Papaya (Carica papaya L.) is a well-known fruit plant with high economic value. The uniformity of germination and initial growth must be considered to get normal mature plants. Using appropriate seedling media and synthetic auxin can support the germination and initial growth of papaya seedlings. This study aimed to determine the effect of the type of seedling media and the duration of immersion of synthetic auxin, and the combination that had a positive effect on germination and initial growth of papaya seedlings. This research was conducted in Badranrejo, Kemiri, Mojosongo, Boyolali, and the Laboratory of the Faculty of Agriculture, Sebelas Maret University, Surakarta, from April to July 2009. This study used a Completely Randomized Design (CRD) with two treatment factors and three replications. The first factor was the type of seedling media: soil, soil + farmer-produced cow manure (1:1), soil + self-produced cow manure (1:1), and soil + farmer-produced cow manure + self-produced cow manure (1:1:1). The second factor was the immersion time in synthetic auxin: 0 hours, 1 hour, 2 hours and 3 hours. Data were analyzed by analysis of variance and if there was a significant difference, proceed with DMRT 5%. The results showed that the interaction between seedling media and immersion time in synthetic auxin did not occur. Therefore, adding cow manure as a medium is unnecessary, especially for papaya seed germination. Synthetic auxin immersion from 1 to 3 hours did not increase all variables of germination and initial growth of papaya seedlings.","PeriodicalId":145551,"journal":{"name":"Cell Biology and Development","volume":"30 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2017-12-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"127719530","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}