IOSR Journal of Pharmacy and Biological Sciences最新文献

{"title":"Co-Administration of Crude Ethanolic Leaf Extract of Ageratum conyzoides ameliorates Nigerian Bonnylight Crude Oil-Induced Erythrocyte Haemolysis in Female Wistar Rats","authors":"S. Ita, M. Nya","doi":"10.9790/3008-1203057377","DOIUrl":"https://doi.org/10.9790/3008-1203057377","url":null,"abstract":"This study focused on the osmotic fragility of erythrocytes and its indices in rats treated with ethanolic leaf extract of Ageratum conyzoides following the administration of Nigerian Bonnylight crude oil (NBLCO). Twenty female Wistar rats (120-150g body weight) were divided into four groups of five rats each. The rats in group I served as the control group and was oral gavaged 3ml/kg of normal saline; group II gavaged 748.33mg/kg body weight of the extract of A. conyzoides, which was 20% of the LD50 (3741.66mg/kg). Group III was gavaged 3ml/kg body weight of NBLCO. This dose was calculated as 20% of the lethal dose of 14.14 ml/kg. Group IV animals were gavaged 748.33mg/kg body weight of the extract of A. conyzoides, and 3ml/kg body weight of NBLCO. In all cases, doses were applied daily for 31 days according to animal’s most recent body weight. The results showed that NBLCO significantly increased osmotic fragility while reducing erythrocyte parameters and indices evaluated compared with control group (normal saline) and A. conyzoides group (group II) (p<0.05). A. conyzoides supplementation helped in increasing these parameters significantly (p<0.05). The results of this study provides evidence that NBLCO has a damaging effect on blood as it can negatively interfere with the functionality of the red blood cell membranes causing haemolysis and ultimately resulting in anaemia. The co-administration of ethanol leaf extract of A. conyzoides has the potential to mitigate this damaging effect of NBLCO to erythrocytes.","PeriodicalId":14548,"journal":{"name":"IOSR Journal of Pharmacy and Biological Sciences","volume":"1 1","pages":"73-77"},"PeriodicalIF":0.0,"publicationDate":"2017-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"90268362","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Determination of Minimum Inhibitory Concentration for Chitosan Extracted from Metapeuous monoceros Against Gram Positive Bacteria, Gram Negative Bacteria and Fungi.","authors":"M. Hasan, M. Hasan, M. Parvin, Rubaya Khatun, Ahoshan Habib, Toriqul Islam, Anika Bushra","doi":"10.9790/3008-1203055357","DOIUrl":"https://doi.org/10.9790/3008-1203055357","url":null,"abstract":"","PeriodicalId":14548,"journal":{"name":"IOSR Journal of Pharmacy and Biological Sciences","volume":"73 1","pages":"53-57"},"PeriodicalIF":0.0,"publicationDate":"2017-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"90948926","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Electrophoretic Profile of Protein and Isozymes in Leaf Gall Of Pongamia Pinnata (L.)","authors":"R. Choudhary, Sanjay Kumar, B. Kumar, Devendra Kumar","doi":"10.9790/3008-1203060104","DOIUrl":"https://doi.org/10.9790/3008-1203060104","url":null,"abstract":"In the present investigation the electrophoretic studies of protein and isozymes (peroxidase and polyphenol oxidase) in leaf galls of Pongamia pinnata induced by Aceria pongamiae were carried out. The results showed some variation in SDS-protein and isozymes activities banding patterns among leaf galls and normal leaf. Total twenty bands were observed in each lane of galled leaf sample, in comparison one less band was observed in normal leaf crude protein profiling of experimental plant. We observed that the intensity of protein bands was higher in galled leaf as compare to normal leaf protein. Intensity of polyphenol oxidase and peroxidase activity was found to be very high in galled leaves as compared to their normal counterparts.","PeriodicalId":14548,"journal":{"name":"IOSR Journal of Pharmacy and Biological Sciences","volume":"21 1","pages":"01-04"},"PeriodicalIF":0.0,"publicationDate":"2017-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"78264109","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Anti-Cancer Therapy: Chlorogenic Acid, Gallic Acid and Ellagic Acid in Synergism","authors":"Archana Moon","doi":"10.9790/3008-1203064852","DOIUrl":"https://doi.org/10.9790/3008-1203064852","url":null,"abstract":"Phytochemicals have been used as effective agents for treating illnesses. Its use has been widely increased in the present scenario where commercial drugs are carrying a huge burden of side effects. Many phytochemicals have potent anticancer properties. The present study was undertaken to study the synergistic effect of anticancer phytochemicals Chlorogenic acid, Gallic acid and Ellagic acid on MDA MB 231 Breast cancer cells. The study was evaluated using cytotoxic assay such as MTT assay and migration assay via scratch well assay. The results demonstrate that the selected phytochemicals are highly cytotoxic to the cells and thus, synergistic studies were performed and the most cytotoxic combinations were proceeded with further assay which reveals that there is no migration observed in the presence of selected phytochemical combinations, thus revealing that treatment must be anti-metastatic.","PeriodicalId":14548,"journal":{"name":"IOSR Journal of Pharmacy and Biological Sciences","volume":"93 1","pages":"48-52"},"PeriodicalIF":0.0,"publicationDate":"2017-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"90553979","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A Chack List of Penaeid Prawns Found In Indian Water with Their Distribution","authors":"A. Chanda","doi":"10.9790/3008-1203052631","DOIUrl":"https://doi.org/10.9790/3008-1203052631","url":null,"abstract":"Present study is an attempt to up to date the taxonomic information of prawns found in Indian water under family Penaeidae Rafinesque – Schmaltz, 1815. Species composition and their distribution in Indian water is the main part of the work. Family Penaeidae is represented by 25 genera of which 17 genera and 78 species has been recorded from Indian water.","PeriodicalId":14548,"journal":{"name":"IOSR Journal of Pharmacy and Biological Sciences","volume":"3 1","pages":"26-31"},"PeriodicalIF":0.0,"publicationDate":"2017-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"82458075","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Ethnobotanical Importance Of Zingiber In The Imphal Valley District, Manipur With Zingiber Chrysanthum Roscoe as A New Record For Manipur.","authors":"N. Devi, A. Das, P. Singh","doi":"10.9790/3008-1203072932","DOIUrl":"https://doi.org/10.9790/3008-1203072932","url":null,"abstract":"The Zingiberaceae is rich in species used as traditional medicines or spices, but extensive information about their chemistry and pharmacology is available for ginger ( Zingiber officinale). Throughout the history of formal ethnobotany, medicinal plants have been an area of keen interest. Zingiber chrysanthum Roscoe has been reported for the first time from Manipur. Detailed description of the plant is provided. The plant was found growing with a small population in the forest margin and foothills of Imphal Valley, Manipur.","PeriodicalId":14548,"journal":{"name":"IOSR Journal of Pharmacy and Biological Sciences","volume":"4 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2017-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"81565535","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Understanding Embryonic Stem Cell Pluripotency Through Its Key Players","authors":"R. Govindan","doi":"10.9790/3008-1203050512","DOIUrl":"https://doi.org/10.9790/3008-1203050512","url":null,"abstract":"Certain transcription factors, cofactors, chromatin regulators are identified as the key players in establishing the embryonic stem cell ground state. The activation and silencing of genes in response to potency and lineage development act as the regulatory circuits. Insights into these regulatory circuitory have uncovered fundamental genetic and epigenetic mechanisms that control gene expressions to maintain the ground state. At one end of the circuit are the core transcription factors that self regulate their own gene expression. These factors further activate transcription of genes involved in pluripotency and contribute to the arrest of expression of lineage specific genes. The core transcription factors mediate binding of the enhancers with signaling factors there by extending the circuit to communicate the signals directly to the genes regulated by the core factors. The core factors also create an undifferentiated state through chromatin regulators that remodify the regulatory gene sequences of specific lineages to inactive states. Understanding the embryonic stem cell ground state in terms of its various regulatory aspects serves as both a baseline for understanding the changes that occur as cells differentiate and develop and as a means to understand the basic biology of these cells. It also helps in understanding the mechanisms behind induced pluripotent cells in vitro culture conditions that might contribute to stem cell based translational medicines.","PeriodicalId":14548,"journal":{"name":"IOSR Journal of Pharmacy and Biological Sciences","volume":"30 1","pages":"05-12"},"PeriodicalIF":0.0,"publicationDate":"2017-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"91185611","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Association between Elevated Serum Ferritin and HBA1C in Type 2 Diabetes Mellitus","authors":"Priyanka Vaitla, Sabiullah, N. Vani","doi":"10.9790/3008-1203065759","DOIUrl":"https://doi.org/10.9790/3008-1203065759","url":null,"abstract":"Background and objectives : Diabetes is a disease of metabolic dysregulation. Recent studies have emphasized the role of serum ferritin in insulin resistance and the incidence of diabetes mellitus. However, the role of ferritin as a marker of iron overload in pancreatic damage and peripheral insulin resistance or its role as an inflammatory marker is not clear. The aim of this study is to establish an association between serum ferritin, FBS and HbA1c in type 2 diabetes mellitus and to evaluate the role of serum ferritin on glycemic status. Methods : A case control study was done with 90 type 2 DM patients. FBS,PLBS, Hb%, HbA1c% and serum ferritin were measured. Results : Significant hyperglycemia was observed in the cases compared to the controls. Mean ±SD of FBS, PLBS, HbA1c and serum ferritin were highest in group with poor glycemic control. Interpretations and conclusion : Higher positive correlation of serum ferritin with HbA1c shows that dysglycemia affects ferritin levels possibly due to inflammation or oxidative stress or a combination of two. Iron may negatively impact on glycemic control. Also in the presence of hyperglycemia and inflammation, iron may contribute to the development and progression of oxidative injury.","PeriodicalId":14548,"journal":{"name":"IOSR Journal of Pharmacy and Biological Sciences","volume":"18 1","pages":"57-59"},"PeriodicalIF":0.0,"publicationDate":"2017-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"81050610","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Antiinflammatory activity of Flavonoids isolated from Rhus Mysorensis","authors":"G. Rani, Singara Charya Ma, M. Viswanadham, M. Thupurani","doi":"10.9790/3008-1203073740","DOIUrl":"https://doi.org/10.9790/3008-1203073740","url":null,"abstract":"Inflammation is part of the complex biological response of body tissues to harmful stimuli, such as pathogens, damaged cells, or irritants, and is a protective response involving immune cells, blood vessels, and molecular mediators. Medicinal plants have been long lastingly recognized as potential sources of natural drugs. The present study was framed to evaluate anti-inflammatory activity of the compounds isolated from Rhus mysorensis. The study was carried out using in vitro and in vivo methods. In vitro anti inflammatory activity of isolated compounds was screened using egg albumin. The inhibition of protein denaturation was found to be high 90.5, 84.1with the compounds 2 and 4 at 100μg/mL respectively. Compounds 2 and 4 found active in reducing the paw volume 0.88±0.26, 1.35±0.11 at fourth hour of treatment.","PeriodicalId":14548,"journal":{"name":"IOSR Journal of Pharmacy and Biological Sciences","volume":"36 1","pages":"37-40"},"PeriodicalIF":0.0,"publicationDate":"2017-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"82664400","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Isolation and Identification of Multi-drug resistant Pseudomonas aeruginosa from Burn Wound Infection in Chittagong City, Bangladesh","authors":"Pankqj Deb Nath, Rocky Chowdhury, K. Dhar, T. Dhar, S. Dutta","doi":"10.9790/3008-1203054347","DOIUrl":"https://doi.org/10.9790/3008-1203054347","url":null,"abstract":"Pseudomonas aeruginosa is an important infectious agent which causes nosocomial infections in immune-compromised patients, especially among burnt patients. In recent times, it has emerged as a far-flung Multi-Drug Resistant (MDR) pathogen which needs regular and periodic antibiotic susceptibility surveillance. The aim of this study was to isolate and identify MDR-P. aeruginosa from the burn patients held in the burn unit of Chittagong Medical College Hospital (CMCH) for ~2 months under treatment andto determine their antimicrobial resistance pattern for some commonly prescribed antibiotics. P. aeruginosa isolates were recovered from ten random wound swab samples of burnt patients through selective growth on MacConkey and Cetrimide agar media. Selected isolates were subjected to cultural, morphological and biochemical characterization. Antibiotic susceptibility patterns of the P. aeruginosa isolates were investigated by standard disk diffusion method of Kirby-Bauer. We assessed their antibiotic sensitivity patterns employing Amoxicillin, Ampicillin, Ceftazidime, Cephalexin, Chloramphenicol, Ciprofloxacin, Gentamicin, and Tetracycline. Two isolates of P. aeruginosa exhibited similar biochemical profiles but differ in cultural characteristics and antibiotypes; hence recognized as separate strains: P. aeruginosa CU1 and P. aeruginosa CU2. P. aeruginosa CU1 was found to be an MDR strain and showed resistance to all the test antibiotics. Not only the isolate exhibited resistance to ampicillin, amoxicillin, cephalexin, ciprofloxacin and tetracycline, but also showed remarkable resistance against two recommended antipseudomonal drugs: gentamicin and ceftazidime. Despite not being an MDR, P. aeruginosa CU2 exhibited resistance against amoxicillin, ampicillin and gentamicin. Though this report covered a small number of samplings and the results represent only a tiny fraction of total burnt patients of the Chittagong city; our findings are auguring enough considering public health concern which might spur care from public health management system of the nation.","PeriodicalId":14548,"journal":{"name":"IOSR Journal of Pharmacy and Biological Sciences","volume":"73 1","pages":"43-47"},"PeriodicalIF":0.0,"publicationDate":"2017-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"82195020","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}