Japanese journal of medical science & biology最新文献_第6页

HLA-A and -B genotyping in living related one haploidentical kidney. 活亲缘单倍肾HLA-A和-B基因分型。

Japanese journal of medical science & biology Pub Date : 1996-08-01 DOI: 10.7883/yoken1952.49.187

S Shintaku, Y Fukuda, A Kimura, S Hoshino, T Asahara, K Dohi

引用次数: 0

Schistosoma mansoni: the ultrastructure of larval morphogenesis in Biomphalaria glabrata and of associated host-parasite interactions. 曼氏血吸虫:光光生物phalaria glabrata幼虫形态发生的超微结构及相关寄主-寄生虫相互作用。

Japanese journal of medical science & biology Pub Date : 1996-08-01 DOI: 10.7883/yoken1952.49.129

S C Pan

{"title":"Schistosoma mansoni: the ultrastructure of larval morphogenesis in Biomphalaria glabrata and of associated host-parasite interactions.","authors":"S C Pan","doi":"10.7883/yoken1952.49.129","DOIUrl":"https://doi.org/10.7883/yoken1952.49.129","url":null,"abstract":"An electron microscopic study has been carried out to describe the transformation of the miracidium of S. mansoni into the mother sporocysts in the susceptible B. glabrata and the associated host-parasite interactions. The miracidium enters the snail host without morphological alterations. Within 3 hr after entering, all the ciliary epidermal plates of the miracidium are discarded. A new tegument is quickly formed by 5 hr postinfection by the expansion of epidermal ridges. The rapid formation of the new tegument reflects the participation of membrane-bound vesicles in the ridge cytons. The membranes of these vesicles become the new tegument membranes with the discharge of their electron-dense contents into the snail tissues. The vesicular contents discharged into the tissues apparently prevent snail amoebocytes (phagocytes) from attachment to the parasite tegument and thus prevent their interference with the further development of the postmiracidium. If a postmiracidium fails to mobilize membrane-bound vesicles in the formation of tegument, the parasite becomes surrounded by closely attached concentric layers of fibroblasts formed by amoebocytes and histiocytes within 24 hr. The membrane-bound vesicles are present in small numbers in the ridge cytons of the miracidium and become numerous in the postmiracidium stage and with many migrate to the ridges through connecting bridges within 24 hr. By 3 days postinfection when extensive microvilli have formed on the tegument the vesicles have disappeared and are replaced by mitochondria, ribosomes and complex carbohydrate particles. Many fibroblasts in the snail connective tissues have phagocytic capacities and are regarded as snail tissue histiocytes or fixed amoebocytes that eventually may become hypertrophic and detached.","PeriodicalId":14531,"journal":{"name":"Japanese journal of medical science & biology","volume":"49 4","pages":"129-49"},"PeriodicalIF":0.0,"publicationDate":"1996-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"20037837","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 25

Cloning and nucleotide sequences of iron and copper-zinc superoxide dismutase genes of Legionella pneumophila and their distribution among Legionella species. 嗜肺军团菌铁和铜锌超氧化物歧化酶基因的克隆、核苷酸序列及其在军团菌中的分布。

Japanese journal of medical science & biology Pub Date : 1996-08-01 DOI: 10.7883/yoken1952.49.167

J Amemura-Maekawa, F Kura, H Watanabe

{"title":"Cloning and nucleotide sequences of iron and copper-zinc superoxide dismutase genes of Legionella pneumophila and their distribution among Legionella species.","authors":"J Amemura-Maekawa, F Kura, H Watanabe","doi":"10.7883/yoken1952.49.167","DOIUrl":"https://doi.org/10.7883/yoken1952.49.167","url":null,"abstract":"A facultative intracellular parasite Legionella pneumophila has two kinds of superoxide dismutase (SOD), iron-containing superoxide dismutase (Fe-SOD) and copper,zinc-containing one (Cu,Zn-SOD). We cloned both SOD genes of L. pneumophila and determined their DNA sequences. The Fe-SOD gene (sodB), isolated by functional complementation of a SOD-deficient Escherichia coli strain, encoded a protein of 192 amino acids conserving the Fe-SOD-specific amino acid residues. A clone containing entire Cu,Zn-SOD gene (sodC) was constructed by connecting two contiguous DNA fragments; one with a lower part of the gene was obtained by colony hybridization with a probe acquired by polymerase chain reaction (PCR) with degenerate oligonucleotide primers corresponding to conserved regions of known Cu,Zn-SOD genes and the other with an upper part of the gene was by IPCR (inverted PCR). The sodC gene encoded a protein of 162 amino acids, of which the first 20 amino acids inferred a signal peptide similar to other bacterial Cu,Zn-SODs reported previously. Both clones expressed their SOD activities in E. coli K-12 through their own plausible promoters. We examined for SOD genes on chromosomes of several Legionella species. All chromosomes were hybridized with Fe-SOD gene of L. pneumophila, but Cu,Zn-SOD gene did not hybridize to the chromosomes of other than L. pneumophila strains.","PeriodicalId":14531,"journal":{"name":"Japanese journal of medical science & biology","volume":"49 4","pages":"167-86"},"PeriodicalIF":0.0,"publicationDate":"1996-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"20037839","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 6

Hematological impairments in recurrent Plasmodium vivax infected patients. 间日疟原虫感染复发患者的血液学损害。

Japanese journal of medical science & biology Pub Date : 1996-08-01 DOI: 10.7883/yoken1952.49.151

R Selvam, G Baskaran

{"title":"Hematological impairments in recurrent Plasmodium vivax infected patients.","authors":"R Selvam, G Baskaran","doi":"10.7883/yoken1952.49.151","DOIUrl":"https://doi.org/10.7883/yoken1952.49.151","url":null,"abstract":"The hematological parameters were assayed in Plasmodium vivax patients with only one infection, two infections, three infections and more than three malarial infections during a period of six months. A steady fall in the levels of hemoglobin as well as packed cell volume (PVC) level was observed with increasing number of infections. The malarial patients showed a progressive decrease in RBC level with increasing number of attacks. The decrease in the hematological indices was statistically significant at all levels of parasitemia. There was a marked increase in the osmotic fragility of the malarial erythrocytes when compared to that of controls. During repeated malarial attacks, significant decrease in MCH (p < 0.05) and MCH (p < 0.01) and increase in the MCV level (p < 0.05) and Heinz body formation (p < 0.001) were observed. Parasite density significantly influenced the fragility of the erythrocytes, Heinz body formation, MCV, MCH and MCHC levels. Thus, the erythrocytes of the patients repeatedly infected with Plasmodium vivax parasite are subjected to structural and functional impairment, ultimately culminating in anemia.","PeriodicalId":14531,"journal":{"name":"Japanese journal of medical science & biology","volume":"49 4","pages":"151-65"},"PeriodicalIF":0.0,"publicationDate":"1996-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.7883/yoken1952.49.151","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"20037838","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 20

Acid sensitivity as affected by physico-chemical stresses in Aeromonas hydrophila. 嗜水气单胞菌理化胁迫对酸敏感性的影响。

Japanese journal of medical science & biology Pub Date : 1996-06-01 DOI: 10.7883/yoken1952.49.95

A Parvis, R Majibur, B Shakhawat, R Arifur, H Ashfaque

{"title":"Acid sensitivity as affected by physico-chemical stresses in Aeromonas hydrophila.","authors":"A Parvis, R Majibur, B Shakhawat, R Arifur, H Ashfaque","doi":"10.7883/yoken1952.49.95","DOIUrl":"https://doi.org/10.7883/yoken1952.49.95","url":null,"abstract":"This work focuses mainly on the effect of NaCl along with other mono and divalent anions and cations at pH 4 on survival and virulence properties of Aeromonas hydrophila. To find the optimum stress condition, effects of several other physical factors on NaCl induction were also assayed. A. hydrophila collected from International Center for Diarrheal Disease Research, Bangladesh (ICDDR,B) was found to be more sensitive to pH 4.0 when grown in media containing 200 mM or more salt. Induction of acid sensitivity was rapidly gained in NaCl-supplemented broth at 37 C. There was only slight sensitization after 5 min, but a marked effect was observed within 30 min. Uninduced A. hydrophila gave an average of 40% survival after exposure to pH 4.0 for 5 min. However, NaCl-induced cells of this organism reduced the survival to a great extent having an average of 0.06%. Either increase in salt concentration (100 mM through 400 mM) or decrease (50 mM) resulted in decrease in percent survival when exposed to gradually increased concentrations of salt. The most effective dose for induction was 400 mM NaCl salt. NaCl-induction was found to be more pronounced in log-phase organisms. All other monovalent and divalent anions and cations tested were almost as effective as NaCl except KCl.","PeriodicalId":14531,"journal":{"name":"Japanese journal of medical science & biology","volume":"49 3","pages":"95-102"},"PeriodicalIF":0.0,"publicationDate":"1996-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"19912549","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

High-level expression of Bacillus thuringiensis phosphatidylinositol-specific phospholipase C by the Bacillus brevis host-vector system. 短芽孢杆菌宿主载体系统高效表达苏云金芽孢杆菌磷脂酰肌醇特异性磷脂酶C。

Japanese journal of medical science & biology Pub Date : 1996-06-01 DOI: 10.7883/yoken1952.49.103

T Kobayashi, H Tamura, R Taguchi, S Udaka, H Ikezawa

引用次数: 4

Influenza virus infections in Taiwan from 1979 to 1995. 1979年至1995年台湾流感病毒感染情况。

Japanese journal of medical science & biology Pub Date : 1996-06-01 DOI: 10.7883/yoken1952.49.77

R K Tseng, H Y Chen, C B Hong

引用次数: 12

Survey for Pneumocystis carinii infection of wild-born and laboratory-bred monkeys by indirect immunofluorescence and cyst-staining methods. 间接免疫荧光法和包囊染色法调查野生和实验室饲养猴子卡氏肺囊虫感染情况。

Japanese journal of medical science & biology Pub Date : 1996-06-01 DOI: 10.7883/yoken1952.49.113

M Fujita, T Furuta, S Kojima, T Kurata, Y Yoshikawa

引用次数: 1

Titration of human cytomegalovirus (HCMV) DNA in urine by combined use of PCR and microplate hybridization in a renal transplant patient with HCMV pneumonitis. PCR和微孔板杂交联合应用于HCMV肺炎肾移植患者尿液中人巨细胞病毒DNA的测定。

Japanese journal of medical science & biology Pub Date : 1996-06-01 DOI: 10.7883/yoken1952.49.121

K Meigata, R Hondo, A Fujima, M Shinkai-Shibata, S Itoh, K Kikuchi, Y Ando, N Ichikawa, Y Nomura, K Watanabe, H Degawa, Y Beck, S Tomikawa, T Nagao, H Uchida

{"title":"Titration of human cytomegalovirus (HCMV) DNA in urine by combined use of PCR and microplate hybridization in a renal transplant patient with HCMV pneumonitis.","authors":"K Meigata, R Hondo, A Fujima, M Shinkai-Shibata, S Itoh, K Kikuchi, Y Ando, N Ichikawa, Y Nomura, K Watanabe, H Degawa, Y Beck, S Tomikawa, T Nagao, H Uchida","doi":"10.7883/yoken1952.49.121","DOIUrl":"https://doi.org/10.7883/yoken1952.49.121","url":null,"abstract":"We titrated human cytomegalovirus (HCMV) DNA in urine specimens obtained from 14 healthy individuals and a renal transplant patient with HCMV pneumonitis by modifying the method for titration of varicella-zoster virus DNA previously described (1,2). Of 14 HCMV seropositive healthy individuals, 13 had HCMV DNA under the detection limit of 10(2.0) copies/ml, whereas one person had 10(2.0) copies/ml. The viral DNA in urine samples was at a low level in healthy individuals with latent infection. In a case with HCMV pneumonitis after renal transplantation, the amount of HCMV DNA in urine gradually increased from the level under 10(2.0) copies/ml and reached a peak of 10(4.7) copies/ml one month prior to the manifestation of pneumonitis. It, thereafter, decreased with the course of clinical remission, and finally settled at under 10(2.0) copies/ml. Serial titrations of HCMV DNA in urine specimens proved to be useful in identifying recipients at risk of developing active HCMV infection after renal transplantation and as a guide for treatment of patients.","PeriodicalId":14531,"journal":{"name":"Japanese journal of medical science & biology","volume":"49 3","pages":"121-7"},"PeriodicalIF":0.0,"publicationDate":"1996-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.7883/yoken1952.49.121","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"19914350","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 4

Investigation for the prevalence of Helicobacter pylori infection in patients with gastric carcinoma in Madras, India. 印度马德拉斯市胃癌患者幽门螺杆菌感染率调查。

Japanese journal of medical science & biology Pub Date : 1996-04-01 DOI: 10.7883/yoken1952.49.49

R Sivaprakash, U A Rao, S P Thyagarajan, B Ramathilakam, V Jayanthi

{"title":"Investigation for the prevalence of Helicobacter pylori infection in patients with gastric carcinoma in Madras, India.","authors":"R Sivaprakash, U A Rao, S P Thyagarajan, B Ramathilakam, V Jayanthi","doi":"10.7883/yoken1952.49.49","DOIUrl":"https://doi.org/10.7883/yoken1952.49.49","url":null,"abstract":"The most common type of gastric carcinoma, namely the intestinal type, has been proposed to result from a precancerous process in which chronic gastritis, atrophy, intestinal metaplasia and dysplasia develop in a sequential manner. Helicobacter pylori is considered as the main cause of chronic atrophic gastritis and thus may play a role in the gastric carcinogenesis process. The present study was aimed at investigating the prevalence of H. pylori in the gastric carcinoma cases. Urease tests and ELISA developed in our laboratory and culture were used to assess the prevalence of H. pylori in the study group. The positivity of H. pylori by various tests ranged from 56.0 to 62.6% in the gastric carcinoma group and 37.3 to 46.6% in the control subjects, the difference being statistically significant. This suggests that H. pylori infection could be associated with an increased risk for gastric carcinoma, but only a very small percentage of the infected persons develops gastric carcinoma. Therefore, it is suggested that along with other critical risk factors, H. pylori may act as a cofactor in the pathogenesis of gastric carcinoma.","PeriodicalId":14531,"journal":{"name":"Japanese journal of medical science & biology","volume":"49 2","pages":"49-56"},"PeriodicalIF":0.0,"publicationDate":"1996-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.7883/yoken1952.49.49","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"19775100","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 20