Investigación Clínica最新文献

{"title":"CircRNA_104293 targets miR-497-5p to inhibit the mTOR/STAT3 pathway and mitigate inflammation in Crohn’s disease.","authors":"Xuerui Yin, Gaigai Huang, C Zheng, Yu Zhou, Rong Chen","doi":"10.54817/ic.v66n4a02","DOIUrl":"https://doi.org/10.54817/ic.v66n4a02","url":null,"abstract":"Crohn’s disease (CD) is a chronic inflammatory bowel disease driven in part by dysregulation of the mTOR/STAT3 signaling pathway, where mTOR activates STAT3 via the PI3K/AKT cascade. Circular RNAs (circRNAs) have recently emerged as essential regulators in inflammatory processes, al-though their specific roles in CD remain largely unexplored. In this study, cir-cRNA expression profiles from CD patients and healthy controls were analyzed, revealing a significant upregulation of circRNA_104293 in CD tissues. Function-al investigations demonstrated that knockdown of circRNA_104293 reduced in-flammatory cytokine production and DNA damage markers, and decreased cell apoptosis. Bioinformatic analysis and experimental validation confirmed a di-rect interaction between circRNA_104293 and miR-497-5p. Furthermore, miR-497-5p inhibition reversed the anti-inflammatory effects of circRNA_104293 si-lencing. Notably, both rapamycin (an mTOR inhibitor) and miR-497-5p mimics suppressed the mTOR/STAT3 pathway and alleviated inflammatory responses. These findings suggest that the circRNA_104293/miR-497-5p axis contributes to CD progression by modulating the mTOR/STAT3 pathway, highlighting its potential as a novel therapeutic target for the treatment of Crohn’s disease.","PeriodicalId":14515,"journal":{"name":"Investigación Clínica","volume":"4 66","pages":"365-365"},"PeriodicalIF":0.0,"publicationDate":"2025-12-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://produccioncientificaluz.org/index.php/investigacion/article/download/44868/53695/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147918219","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Rutin as a potential therapeutic agent for arsenic-induced testicular damage: Antioxidant, anti-inflammatory, and anti-apoptotic effects.","authors":"Yunyun Wang, Zengrong Hua, Jin Qing Hui, Dandan Qiu","doi":"10.54817/ic.v66n4a05","DOIUrl":"https://doi.org/10.54817/ic.v66n4a05","url":null,"abstract":"Arsenic exposure is associated with male reproductive toxicity, in-cluding impaired spermatogenesis, reduced sperm quality, and disrupted hormone levels. Rutin, a natural flavonoid, has demonstrated protective effects in various or-gans owing to its antioxidant, anti-inflammatory, and antiapoptotic properties.This study aimed to assess the efficacy of rutin in ameliorating testicular toxicity caused by sodium arsenite in laboratory rats.Sprague-Dawley rats were randomly assigned to normal, arsenic control, rutin (25, 50, and 100 mg/kg), and Co-enzyme Q10 (10 mg/kg) treatment groups. Testicular damage was induced by oral administration of sodium arsenite (10 mg/kg, for two days). Rutin and Co-enzyme Q10 were adminis-tered orally for 15 and seven days, respectively. The serum hormone levels, sperm pa-rameters, testicular mitochondrial enzymes, oxidative stress markers, inflammatory cytokines, apoptotic proteins, and histopathology were assessed.Arsenic exposure significantly decreased (p<0.001) sperm parameters (count, motility, and viabil-ity), serum hormone levels (follicle-stimulating hormone, luteinizing hormone, and testosterone), and mitochondrial enzyme activity. Rutin (50 and 100 mg/kg) sig-nificantly (p<0.01 and p<0.001) attenuated arsenic-induced alterations in a dose-dependent manner, improving organ weight, sperm parameters, and hormone lev-els. Rutin also improved mitochondrial complex activity and testicular architecture. In contrast, elevated oxidative stress (reduced glutathione, superoxide dismutase, nitric oxide, and lipid peroxidation), inflammation (tumor necrosis factor-alpha, interleukin-6, and interleukin-1β), and apoptosis (caspase-3 and caspase-9 protein expression) were ameliorated by rutin. In conclusion, rutin demonstrated signifi-cant protective effects against sodium arsenite-induced testicular toxicity in rats by reducing oxidative stress, inflammation, and apoptosis. These findings suggest that rutin has therapeutic potential in mitigating arsenic-related reproductive toxicity.","PeriodicalId":14515,"journal":{"name":"Investigación Clínica","volume":"66 4","pages":"408-408"},"PeriodicalIF":0.0,"publicationDate":"2025-12-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://produccioncientificaluz.org/index.php/investigacion/article/download/44886/53723/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147896642","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Lycopene regulates the formation of calcium oxalate kidney stones by modulating reactive oxygen species(ROS) and NF-κB pathways.","authors":"Liangwen Ye, Yuhang Tang, Zhijie Zhang, Xiangyi Hou, Wei Xu, Xianghui Suo, Li Zhang","doi":"10.54817/ic.v66n2a07","DOIUrl":"https://doi.org/10.54817/ic.v66n2a07","url":null,"abstract":"This study aims to determine whether lycopene can reduce oxi-dative stress and inflammatory damage in HK-2 cell cultures induced by cal-cium oxalate crystallization through the modulation of reactive oxygen species (ROS) and the NF-κB signalling pathway. Cell cultures were divided into four groups: The control group, the Model group (COM + oxalic acid), and two Lyco-pene intervention groups (COM + oxalic acid + 5/10 μmol/L lycopene). After 24 hours of culture, viability, LDH, oxidative and anti-oxidative parameters, mi-tochondrial membrane potential, MCP-1, IL -6, apoptosis and related proteins, and activation and expression of NF-κB were determined by adequate methods. When compared to the control group, the model group exhibited decreased cell activity (p<0.001) and GSH and SOD antioxidant capacity (p<0.05), along-side a significant rise in LDH, MDA, and the release of inflammatory mediators MCP-1 and IL -6 (p<0.05). The levels of protein expression for NF-κB, OPN, Bax, Cyt C, and active Caspase-3 were increased (p<0.05), whereas Bcl-2 protein expression significantly diminished (p<0.05). The mitochondrial membrane potential decreased. Lycopene intervention reduced the damage to HK-2 cells (p<0.05), accompanied by decreased levels of LDH, MDA, and inflammatory factors MCP-1 and IL -6 (p<0.05), and increased GSH and SOD antioxidant capacity (p<0.05). The mitochondrial membrane potential was observed to in-crease. No significant changes were observed in the expression of NF-κB. The expressions of OPN, Bax, Cyt C, and Caspase-3 decreased (p<0.05), whereas the level of Bcl-2 protein expression increased. In conclusion, lycopene decreas-es cellular damage by inhibiting lipid peroxidation induced by calcium oxa-late crystals and oxalate, enhancing intracellular antioxidant enzyme activity, modulating ROS and NF-κB inflammatory pathways, improving mitochondrial integrity, and exerting anti-inflammatory effects through the inhibition of the mitochondrial-mediated Bax/Caspase-3 signalling pathway.","PeriodicalId":14515,"journal":{"name":"Investigación Clínica","volume":"66 2","pages":"205-216"},"PeriodicalIF":0.0,"publicationDate":"2025-06-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.produccioncientificaluz.org/index.php/investigacion/article/download/43927/52060/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147332836","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"comPhillygenin reduced neuropathic pain by inhibiting the rats’ TLR4/MyD88/NF-κB pathway.","authors":"Lei Hua, Waiping Zhou, Mengjie Li, Rong-Chun Li","doi":"10.54817/ic.v66n1a01","DOIUrl":"https://doi.org/10.54817/ic.v66n1a01","url":null,"abstract":"To elucidate the effects of phillygenin (PHI) and the potential mechanism on TLR4 and MyD88/NF-κB signalling in neuropathic pain in an-imal studies, chronic constriction injury (CCI) models were constructed for neuropathic pain induction using male Sprague-Dawley rats. PHI (20 mg/kg) was delivered through intragastric administration. Von Frey and Hargreaves tests were implemented to determine the 50% paw-withdrawal threshold (PWT) and paw-withdrawal latency (PWL). A nitric oxide (NO) assay was used for NO level detection, and an ELISA assay was employed to measure the expression of proinflammatory cytokines. Western blotting and RT-qPCR were conducted for protein and mRNA level detection. Treatment with PHI significantly enhanced 50% of PWT and PWL. PHI significantly decreased the levels of NO and reduced the levels of TNF-α, IL -1β, and IL -6. PHI also downregulated TLR4 and MyD88 expressions and inhibited the phosphorylation of NF-κB.PHI ameliorated in-flammatory status and alleviated neuropathic pain in CCI rats, targeting TLR4 and suppressing MyD88/NF-κB signalling.","PeriodicalId":14515,"journal":{"name":"Investigación Clínica","volume":"66 1","pages":"4-15"},"PeriodicalIF":0.0,"publicationDate":"2025-03-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.produccioncientificaluz.org/index.php/investigacion/article/download/43610/51513/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147878609","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Expresión de receptores de andrógeno en pacientes con carcinoma de mama del subtipo triple negativo.","authors":"Allen Hung, J. Gutiérrez, Janelle Gasia, Yusmary Gil, José Guanipa, Julio Castro, A. Reigosa, Ángel Fernández","doi":"10.54817/ic.v65n1a06","DOIUrl":"https://doi.org/10.54817/ic.v65n1a06","url":null,"abstract":"Breast carcinoma of the triple-negative subtype (TNBC) does not express estrogen, progesterone or human epidermal growth factor recep-tor 2 (HER2) receptors. A subclassification of TNBC has been proposed into four subgroups, one of which is the luminal androgen receptor. The objective of this work was to determine the expression of the androgen receptor (AR) in women with TNBC, diagnosed by immunohistochemistry, at the Hospital Metropolitano del Norte, Valencia, Venezuela. The sample consisted of 191 pa-tients who met the inclusion criteria. The mean age of the patients at the time of diagnosis was 54.91 years. Histologically, the majority of the tumors were moderately differentiated or undifferentiated. The percentage of AR expres-sion was 22% (42 of 191). In the association between AR expression and the Ki-67 proliferative index, a greater number of AR positive cases was observed in tumors with Ki-67 expression of less than 40%. The AR positivity found in this study represents an intermediate frequency in relation to other investigations. No statistically significant differences were found with the age of the patients and histological grade of the tumors, while the Ki-67 proliferative index was lower for AR+ tumors, compared to TNBC in general.","PeriodicalId":14515,"journal":{"name":"Investigación Clínica","volume":"229 ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-02-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140453272","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Diagnostic value of abdominal ultrasound in patients with acute appendicitis and analysis of the expression of related inflammatory factors.","authors":"Siyuan Yang, Mingyan Wang, Linxin Yang, Ning Lin","doi":"10.54817/ic.v65n1a03","DOIUrl":"https://doi.org/10.54817/ic.v65n1a03","url":null,"abstract":"Appendicitis is an inflammation of the appendix that, if left un-treated, can be life-threatening. Abdominal ultrasound helps diagnose it and differentiate it from other causes of abdominal pain. This study aimed to evalu-ate the diagnostic value of abdominal ultrasound in acute appendicitis (AA) and assess inflammatory factor levels in different types of appendicitis. One hundred patients with AA were selected as the observation group, and 30 pa-tients with simple abdominal pain as the control group. Among the 100 AA patients, 37 (37%) cases had blurred appendiceal boundaries, 24 (24%) cases had fecal calculus in the appendix cavity, 13 (13%) cases had enhanced echo intensity of surrounding fat, 15 (15%) cases presented enlarged outer diameter of the appendix (> 6mm), one (1%) case had peripheral lymphadenopathy, and one (1%) case had peripheral effusion. None of the cases (0%) presented a pe-ripheral mass. The levels of white blood cells (WBC) and inflammatory factors: C-reactive protein (CRP), interleukin-6 (IL -6), and tumor necrosis factor α(TNF-α)) patients with uncomplicated appendicitis were lower than those with suppurative appendicitis, gangrenous appendicitis, or peri-appendiceal abscess (p <0.05). The blurred boundary of the appendix, fecal stones in the appendix cavity, an enlarged outer diameter of the appendix (> 6mm), and an enhanced echogenicity of the surrounding fat are the most common ultrasonic signs of AA. Abdominal ultrasound has an excellent diagnostic value on pathological types of AA. The increase in the level of inflammatory factors can indicate the severity of the disease to a certain extent.","PeriodicalId":14515,"journal":{"name":"Investigación Clínica","volume":"118 ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-02-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140453290","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Microbiota vaginal y cáncer de cuello uterino.","authors":"José Núñez-Troconis, Antonio Molero","doi":"10.54817/ic.v65n1a10","DOIUrl":"https://doi.org/10.54817/ic.v65n1a10","url":null,"abstract":"This narrative review analyzed the relationship between cervical cancer and the vaginal microbiome. It has been estimated that viruses and bac-teria cause 15% of all cancers. The development of molecular biology, genetics, and the use of high-throughput genomic sequencing techniques has allowed us not only to know the phenotypic component but also the genetic component of the vaginal microbiome and obtain a better knowledge and understanding of the behavior of the vaginal flora that was previously impossible to determine. The relation of the alterations in the vaginal flora by women’s habits, immunological status, and socioeconomic level has been established. Literature searches were performed electronically in PubMed, Medline, ISI, DOAJ, Springer, Embase, Web of Knowledge, and Google Scholar for original articles written in English and Sci-elo, Latindex, Imbiomed-L, Redalyc, and Google Scholar for original articles writ-ten in Spanish. The objective of this narrative review was to examine and analyze the literature on the alterations in the vaginal microbiome, their local protective effect and their possible contribution to the development of cervical cancer.","PeriodicalId":14515,"journal":{"name":"Investigación Clínica","volume":"155 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-02-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140453389","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"EDITORIAL\u0000Alcances de la revista Investigación Clínica durante el año 2023.","authors":"E. Ryder","doi":"10.54817/ic.v65n1a00","DOIUrl":"https://doi.org/10.54817/ic.v65n1a00","url":null,"abstract":"The main achievement of the journal in 2023 was an increase in the number of works received, mainly of foreign origin. The rejection percentage was 36%. The evaluation of the work of the members of the Editorial Committee had the collaboration of external referees from national and foreign institutions, especially Venezuelans living abroad. The magazine fulfilled its frequency of four issues with 41 communications in 559 pages, exceeding the number of pages from the previous year. Among the 41 works, the original works in English predominated, both from Venezuela and Latin America and countries on other continents. In evaluating the magazine by international indices, the increase in the Copernicus Index score stands out due to excellent compliance with their classification patterns. There were discrepancies between the different indexes in the citations; Clarivate reported 204, and Sco-pus only 20. Scopus recognizes only 44 citations in the four years (2019-2022), and Scielo recognizes 498 in that same period. The Crossref report was of more than 13,000 accesses in 2023, with Spanish-language Reviews being the most frequent, which suggests that Latin America is the primary source of readers for the magazine. This observation corroborates the results of our website, where the main accesses come from American countries. We hope that in 2024, Investigación Clínica will increase its national and international recognition; it would be a gift to the memory of its founder, Américo Negrette, who would be celebrating the centenary of his birth in 2024.","PeriodicalId":14515,"journal":{"name":"Investigación Clínica","volume":"58 14","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-02-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140453751","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Correlation between the degree of cognitive impairment and emotional state in patients with Alzheimer’s disease.","authors":"Zhichao Qiu, Jingjing Cai, Fanlin Xia","doi":"10.54817/ic.v65n1a04","DOIUrl":"https://doi.org/10.54817/ic.v65n1a04","url":null,"abstract":"This study aimed to investigate the correlation between cogni-tive dysfunction and emotional state in patients with Alzheimer’s disease and then propose intervention strategies. One hundred twenty-five patients with Alzheimer’s disease from June 2019 to May 2022 were selected as the study subjects and divided into two groups based on the degree of cognitive impair-ment, both receiving routine drug treatment and cognitive rehabilitation in-tervention. The Montreal Cognitive Assessment (MoCA) and the Positive and Negative Affect Scale (PANAS) were used to evaluate the cognitive function and emotional status of two groups of patients before the intervention and four and eight weeks of intervention and to analyze the correlations between the two. The results showed statistically significant differences between the two groups MoCA and PANAS scores (P<0.05). Before the intervention, the patient’s MoCA score was positively correlated with the PANAS positive emo-tion score and negatively correlated with the PANAS negative emotion score (P<0.05). After four and eight weeks of intervention, the patient’s MoCA score was positively correlated with the PANAS positive emotion score (P<0.05) and negatively correlated with the PANAS negative emotion score (P<0.05).","PeriodicalId":14515,"journal":{"name":"Investigación Clínica","volume":"171 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-02-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140453321","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Pulmonary toxicity associated with high-dose favipiravir and treatment options: biochemical and histopathological evaluation.","authors":"B. Elma, B. Suleyman, R. Mammadov, B. Yavuzer, Edhem Unver, D. Altuner, T. Coban, Behzat Mokhtare, H. Suleyman","doi":"10.54817/ic.v65n1a08","DOIUrl":"https://doi.org/10.54817/ic.v65n1a08","url":null,"abstract":"Favipiravir is a broad-spectrum antiviral drug that is a viral RNA-dependent RNA polymerase inhibitor. Favipiravir is used in high doses to treat COVID-19 but has a side effect on humans at high doses. The side effects of favipiravir have been associated with oxidative stress in the literature. In this trial, we investigated the biochemical and histopathological effects of lacidip-ine, thiamine pyrophosphate (TTP), and adenosine triphosphate (ATP), drugs with antioxidant properties, on the lung toxicity caused by high-dose favipiravir in rats. The rats were classified into five groups: healthy (HG), favipiravir alone (Fav), lacidipine+favipiravir (LFav), TPP+favipiravir (TFav), and ATP+favipiravir (AFav). Favipiravir (800 mg/kg) was administered twice daily for seven days. Laci-dipine (4 mg/kg), TPP (20 mg/kg), and ATP (25 mg/kg) were administered once daily for seven days. Oxidant (malondialdehyde), non-enzymatic (total glutathi-one), and enzymatic (superoxide dismutase and catalase) antioxidant levels were measured in the excised lung tissues. Furthermore, the tissues were histopatho-logically examined. The systemic administration of high doses of favipiravir in-creased oxidant levels and decreased antioxidant levels in the lung tissue of rats. In parallel, the histopathological examination of the lung tissue revealed the presence of severe mononuclear cell infiltrations in interstitial areas and pronounced lymphoid hyperplasia. Lacidipine exhibited superior efficacy in mit-igating oxidative stress and preventing the decline of antioxidants induced by favipiravir compared with TPP and ATP. Histopathologically, the lacidipine admin-istration significantly reduced lung oxidative damage. TTP moderately reduced severe favipiravir-associated lung injury. However, ATP was ineffective against fa-vipiravir-associated lung injury. Lacidipine offers more therapeutic benefits than TPP in treating oxidative lung injury caused by high doses of favipiravir.","PeriodicalId":14515,"journal":{"name":"Investigación Clínica","volume":"65 7","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-02-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140453574","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}