Investigación Clínica最新文献_第3页

Antifúngicos: lo que tenemos, lo que tendremos, lo que queremos. 抗真菌剂：我们有什么，我们将有什么，我们想要什么。

Investigación Clínica Pub Date : 2023-11-22 DOI: 10.54817/ic.v64n4a11

D. Martínez-Méndez, M. Bravo-Acosta, Neomar Semprún-Hernández

引用次数: 0

Nuclear and cytoplasmic expressions of the receptor for advanced glycation end products (RAGE) in the rat central nervous system. 大鼠中枢神经系统高级糖化终产物受体（RAGE）的核表达和细胞质表达。

Investigación Clínica Pub Date : 2023-11-22 DOI: 10.54817/ic.v64n4a07

Jesús A. Mosquera-Sulbaran, A. Pedreáñez, Yenddy Carrero, Catherina Peña

{"title":"Nuclear and cytoplasmic expressions of the receptor for advanced glycation end products (RAGE) in the rat central nervous system.","authors":"Jesús A. Mosquera-Sulbaran, A. Pedreáñez, Yenddy Carrero, Catherina Peña","doi":"10.54817/ic.v64n4a07","DOIUrl":"https://doi.org/10.54817/ic.v64n4a07","url":null,"abstract":"The receptor for advanced glycation end products (RAGE) is a transmembrane protein involved in the induction of inflammatory processes and oxidative stress after interacting with its ligands on the cell surface. Lo-calization on the cell surface is necessary for interaction with the ligands. This study aimed to determine the expression of RAGE in different parts of the nor-mal rat brain and cerebellum using the immunofluorescence technique. Sev-eralcerebral cortex layers (molecular/granular layers: M/GL; pyramidal layer: PL) and the hypothalamus were analyzed, as well as the molecular layer (CML) and the granular layer (CGL) of the cerebellum. Cells with RAGE-positive nu-clei were generally observed in the brain’s cerebral cortex and cerebellum. In the M/GL, cells with different degrees of positivity in the nucleus and cyto-plasm accompanied by RAGE-positive material in the adjacent extracellular space were observed, and RAGE-positive material in the neuropile. Pyramidal neurons presenting various degrees of nuclear RAGE-positive material budding and cells with different degrees of nuclear and cytoplasmic positivity were ob-served in PL. The hypothalamus showed a high number of cells with RAGE-positive granules adjacent to the nucleus and in the cytoplasm; nuclei remained negative. Many positive nuclei were observed in CML; they were scarce in CGL. These data suggest the storage of RAGE at the nuclear and cytoplasmic levels in healthy rats and hypothesize the possible translocation of this molecule to the cell surface in pathological conditions.","PeriodicalId":14515,"journal":{"name":"Investigación Clínica","volume":"51 ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-11-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139249933","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Effects of SU5416 on angiogenesis and the ERK-VEGF/MMP-9 pathway in rat endometriosis. SU5416 对大鼠子宫内膜异位症血管生成和 ERK-VEGF/MMP-9 通路的影响

Investigación Clínica Pub Date : 2023-11-22 DOI: 10.54817/ic.v64n4a05

Danyang Zhao, Qiufang Bao, Lihong Chen, Lie Zheng

{"title":"Effects of SU5416 on angiogenesis and the ERK-VEGF/MMP-9 pathway in rat endometriosis.","authors":"Danyang Zhao, Qiufang Bao, Lihong Chen, Lie Zheng","doi":"10.54817/ic.v64n4a05","DOIUrl":"https://doi.org/10.54817/ic.v64n4a05","url":null,"abstract":"SU5416 is a small molecule vascular endothelial growth factor (VEGF) receptor signal transduction inhibitor, which can block the VEGF re-ceptor autophosphorylation and inhibit receptor tyrosine kinase signal trans-duction, thereby reducing VEGF activity. However, there are few reports about the correlation of SU5416 to the occurrence and angiogenesis in endometrio-sis. In this study, we observed the effects of VEGF receptor inhibitor SU5416 on angiogenesis in endometriosis in rats. Thirty femalespecific-pathogen-free Sprague-Dawley rats were randomly divided into sham operation group (SOG), model group (MG), and SU5416 group (n=10 for each group). In the SOG, only the uterus was cut and sutured, and endometriosis models were established in the MG and SU5416 group by autologous transplantation. The SU5416 group was injected with 15 mg/kg SU5416 intraperitoneally, and the SOG and MG were intraperitoneally injected with an equal volume of normal saline for 6 weeks. The volume of ectopic lesions was lower in the SU5416 group at 42 d postoperatively thanin the MG (p<0.05). The proportion of CD31-positive cells in the endometrial tissue of the SU5416 group was lower than that of the MG (p<0.05); angiopoietin-1 (Ang-1), angiopoietin-2 (Ang-2), laminin-5γ2 (LN-5γ2) and phosphorylation of ERK (P-ERK), VEGF, matrix metalloproteinase (MMP)-2, and MMP-9 protein expressions were lower in the SU5416 groupthan in the MG (p<0.05).VEGFreceptor inhibitor SU5416 can inhibit endometrio-sis angiogenesis and reduce inflammatory response in rats, and its mechanism of action may be related to the down-regulation of the ERK-VEGF/MMP-9 path-way expression.","PeriodicalId":14515,"journal":{"name":"Investigación Clínica","volume":"20 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-11-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139249724","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Stress-associated ovarian damage, infertility, and delay in achieving pregnancy and treatment options. 与压力相关的卵巢损伤、不孕症、怀孕延迟及治疗方案。

Investigación Clínica Pub Date : 2023-11-22 DOI: 10.54817/ic.v64n4a08

Gulsah Gulsah Aynaoglu Yildiz, Omer Erkan Yapca, K. Dinç, Cebrail Gursul, Betul Gundogdu, Mehmet Aktas, Z. Suleyman, S. Bulut, H. Suleyman

{"title":"Stress-associated ovarian damage, infertility, and delay in achieving pregnancy and treatment options.","authors":"Gulsah Gulsah Aynaoglu Yildiz, Omer Erkan Yapca, K. Dinç, Cebrail Gursul, Betul Gundogdu, Mehmet Aktas, Z. Suleyman, S. Bulut, H. Suleyman","doi":"10.54817/ic.v64n4a08","DOIUrl":"https://doi.org/10.54817/ic.v64n4a08","url":null,"abstract":"Abstract. Many types of stress, including psychological stress, nega-tively affect reproductive health. This study aimed to investigate the ef-fects of sertraline (a selective serotonin reuptake inhibitor), cerebrolysin (neuroprotective/neurotrophic), and a combination of both against stress-induced ovarian damage, infertility and pregnancy delay in female rats. The rats were divided into five groups (n=14/each group) as healthy (HG), stress control (StC), stress+sertraline (SS), stress+cerebrolysin (SC), and stress+sertraline+cerebrolysin (SSC). To induce stress, animals (except the HG) were kept in a supine position with their forelimbs and hindlimbs (FIM) tied for one hour. Then, sertraline (20mg/kg) was given orally to the SS. Cerebrolysin (2.5ml/kg) was injected into the SC subcutaneously. Sertraline+cerebrolysin was administered to SSC with the same methods and doses. FIM and drug administration continued for 30 days. Six rats from each group were euthanized with high-dose anesthesia, right and left ovarian tissues were removed, and tissues were examined biochemically and histopathologi-cally. The remaining rats were taken for breeding. Exposure to stress in rats caused an increase in malondialdehyde (MDA), tumor necrosis factor-alpha (TNF-α), interleukin-1β (IL -1β), and interleukin-6 (IL -6) levels and a decrease in total glutathione (tGSH). Stress was related to histopathological damage, infertility, and delayed birth. The sertraline and cerebrolysin combination was the most effective in preventing these changes, with sertraline and cerebroly-sin alone in second and third places, respectively. Regarding efficacy, selective serotonin reuptake inhibitors (SSRIs) and related drugs may be beneficial in treating stress-related ovarian damage, infertility, and delay in pregnancy.","PeriodicalId":14515,"journal":{"name":"Investigación Clínica","volume":"140 ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-11-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139246994","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Effects of focused ultrasound on human cervical cancer HeLa cells in vitro. 聚焦超声对体外人类宫颈癌 HeLa 细胞的影响。

Investigación Clínica Pub Date : 2023-11-22 DOI: 10.54817/ic.v64n4a01

Yanbin Liu, Qun Zhao, Panpan Liu, Yanbin Li, Li’an Yi, Haiping Yan

{"title":"Effects of focused ultrasound on human cervical cancer HeLa cells in vitro.","authors":"Yanbin Liu, Qun Zhao, Panpan Liu, Yanbin Li, Li’an Yi, Haiping Yan","doi":"10.54817/ic.v64n4a01","DOIUrl":"https://doi.org/10.54817/ic.v64n4a01","url":null,"abstract":"Cervical cancer is the fourth most common malignant tumor in women. Many studies have confirmed that early childbirth, prolificacy, HPV infection, and smoking are some risk factors. This article explored the effects of exposing human cervical cancer HeLa cells to different focused ultrasound intensities in vitro. The study employed three groups of cells: 1- a high-intensity treated group, 2- a low-intensity treated group, and 3- a control group. Results showed that after 12 hours of focused ultrasound treatment, the growth inhibi-tion rate of the low-intensity group was 55.6% higher than that of the control group, and the growth inhibition rate of the high-intensity group was 41.2% higher than that of the low-intensity group. Therefore, focused ultrasound had a specific inhibitory effect on the growth of HeLa cells, and the higher the intensity of focused ultrasound, the higher the inhibition rate on cancer cells. In addition, the Cycle Threshold (Ct) values of the three groups of cells before treatment were the same, but the Ct values after treatment had changed. The Ct value of the low-intensity group was 18.1% lower than that of the control group, and the Ct value of the high-intensity group was lower than that of the low-intensity group by 27.8%, showing that focused ultrasound can effectively reduce the activity of HeLa cells in vitro.","PeriodicalId":14515,"journal":{"name":"Investigación Clínica","volume":"197 ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-11-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139249688","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Evaluation of myocardial infarction by a 12-lead routine electrocardiogram: a case report of an ST-segment elevation. 通过 12 导联常规心电图评估心肌梗死：ST 段抬高的病例报告。

Investigación Clínica Pub Date : 2023-11-22 DOI: 10.54817/ic.v64n4a10

Huayong Jin, Lijiang Ding, Binglei Li, Jianming Zhang

{"title":"Evaluation of myocardial infarction by a 12-lead routine electrocardiogram: a case report of an ST-segment elevation.","authors":"Huayong Jin, Lijiang Ding, Binglei Li, Jianming Zhang","doi":"10.54817/ic.v64n4a10","DOIUrl":"https://doi.org/10.54817/ic.v64n4a10","url":null,"abstract":"The spiked helmet sign (SHS) is a type of ST-segment elevation associated with critical cardiac disease and a high risk of death. We report a case of SHS caused by an ECG artifact. A 60-year-old male patient presented to the clinic after suffering an electric shock. The initial 12-lead routine elec-trocardiogram showed an SHS. The patient received appropriate intravenous fluid replacement therapy, and after 30 minutes, the ST-T changes of the 12-lead electrocardiogram were all restored to normal. The patient was discharged after a 24-hour observation period in the emergency room.Recent studies have pointed out that there may be two different types of SHS. One is the mechanical factor, and the other is the significant prolongation of the QT interval. The two types have different clinical significance. In our report, the radial artery of the patient’s right wrist pulsed strongly, and after the occurrence of SHS, the SHS disappeared after adjusting the contact position of the electrode in his right arm. This SHS caused by mechanical traction was an ECG artifact.Although the SHS may be an essential indicator of critical illness, there are mechanical factors that lead to the appearance of ECG artifacts. Therefore, in clinical work, obtaining a complete medical history and primary conditions of the patient at the time of ECG sampling is necessary to help the diagnosis and thus avoid er-roneous treatment.","PeriodicalId":14515,"journal":{"name":"Investigación Clínica","volume":"178 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-11-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139248713","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Inflamasoma, piroptosis y su posible relación con la fisiopatología de la COVID-19 炎症瘤、焦痂病及其与COVID-19病理生理学的可能关系

Investigación Clínica Pub Date : 2020-09-01 DOI: 10.22209/ic.v61n3a07

Adriana Pedreáñez, J. Mosquera, Nelson Muñoz

引用次数: 1