IntraVital最新文献_第5页

Hybrid multiphoton multimodal tomography of in vivo human skin 活体皮肤的混合多光子多模态断层扫描

IntraVital Pub Date : 2012-07-01 DOI: 10.4161/intv.21938

K. Koenig

{"title":"Hybrid multiphoton multimodal tomography of in vivo human skin","authors":"K. Koenig","doi":"10.4161/intv.21938","DOIUrl":"https://doi.org/10.4161/intv.21938","url":null,"abstract":"Clinical multiphoton tomography based on femtosecond near infrared laser pulses for in vivo high-resolution skin imaging has been employed to thousands of volunteers and patients. Two-photon cellular autofluorescence and second harmonic generation of collagen can be detected with single-photon sensitivity and submicron spatial resolution. Also in vivo clinical CARS has been realized to image intratissue lipids and water. Novel developments focus on multimodal hybrid imaging to generate optical tissue biopsies with subcellular resolution, deep-tissue information, and chemical fingerprints. Wide-field imaging tools such as dermoscopes, optical coherence tomographs as well as ultrasound and photoacoustic devices can be integrated. The hybrid tomographs have the potential to trace cosmetics and pharmaceutical components such as sunscreen nanoparticles and anti-aging products in humans. Skin cancer such as malignant melanoma and basal cell carcinoma as well as dermatitis can be detected at an early stage and the efficiency of the treatment can be monitored. These novel hybrid multimodal multiphoton tomographs may become important biopsy-free and label-free imaging tools in personalized medicine, pharmacy, biotechnology as well as cosmetic research.","PeriodicalId":14512,"journal":{"name":"IntraVital","volume":"91 1","pages":"11 - 26"},"PeriodicalIF":0.0,"publicationDate":"2012-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"91193515","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 60

Intravital third harmonic generation microscopy of collective melanoma cell invasion: Principles of interface guidance and microvesicle dynamics. 活体三次谐波显微镜观察黑色素瘤细胞侵袭:界面引导和微泡动力学原理。

IntraVital Pub Date : 2012-07-01 eCollection Date: 2012-01-01 DOI: 10.4161/intv.21223

Bettina Weigelin, Gert-Jan Bakker, Peter Friedl

{"title":"Intravital third harmonic generation microscopy of collective melanoma cell invasion: Principles of interface guidance and microvesicle dynamics.","authors":"Bettina Weigelin, Gert-Jan Bakker, Peter Friedl","doi":"10.4161/intv.21223","DOIUrl":"https://doi.org/10.4161/intv.21223","url":null,"abstract":"Cancer cell invasion is an adaptive process based on cell-intrinsic properties to migrate individually or collectively, and their adaptation to encountered tissue structure acting as barrier or providing guidance. Whereas molecular and physical mechanisms of cancer invasion are well-studied in 3D in vitro models, their topographic relevance, classification and validation toward interstitial tissue organization in vivo remain incomplete. Using combined intravital third and second harmonic generation (THG, SHG), and three-channel fluorescence microscopy in live tumors, we here map B16F10 melanoma invasion into the dermis with up to 600 µm penetration depth and reconstruct both invasion mode and tissue tracks to establish invasion routes and outcome. B16F10 cells preferentially develop adaptive invasion patterns along preformed tracks of complex, multi-interface topography, combining single-cell and collective migration modes, without immediate anatomic tissue remodeling or destruction. The data suggest that the dimensionality (1D, 2D, 3D) of tissue interfaces determines the microanatomy exploited by invading tumor cells, emphasizing non-destructive migration along microchannels coupled to contact guidance as key invasion mechanisms. THG imaging further detected the presence and interstitial dynamics of tumor-associated microparticles with submicron resolution, revealing tumor-imposed conditioning of the microenvironment. These topographic findings establish combined THG, SHG and fluorescence microscopy in intravital tumor biology and provide a template for rational in vitro model development and context-dependent molecular classification of invasion modes and routes.","PeriodicalId":14512,"journal":{"name":"IntraVital","volume":"1 1","pages":"32-43"},"PeriodicalIF":0.0,"publicationDate":"2012-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.4161/intv.21223","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"35967985","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 284

Intravital multiphoton imaging of rhodamine 123 in the rat liver after intravenous dosing 罗丹明123静脉给药后大鼠肝脏的多光子成像

IntraVital Pub Date : 2012-07-01 DOI: 10.4161/intv.21450

Xin Liu, C. A. Thorling, Lu Jin, M. Roberts

{"title":"Intravital multiphoton imaging of rhodamine 123 in the rat liver after intravenous dosing","authors":"Xin Liu, C. A. Thorling, Lu Jin, M. Roberts","doi":"10.4161/intv.21450","DOIUrl":"https://doi.org/10.4161/intv.21450","url":null,"abstract":"Intravital imaging with multiphoton microscopy was used to investigate the hepatic disposition of rhodamine 123 (RH123) in the exposed liver of anesthetized rats after intravenous dosing. The role played by the biliary canalicular transporter P-glycoprotein (P-gp) on the disposition of RH123 was explored by administering a P-gp inhibitor, cyclosporine A prior to RH123 administration. The fluorescence intensity of RH123 was defined by multiphoton microscopy using a femtosecond laser excitation wavelength of 900 nm whereas the autofluorescence at an excitation wavelength of 740 nm was used to define the morphology of the liver acini. Intravital imaging showed that RH123 was rapidly taken up from the sinusoids into hepatocytes but slowly eliminated from the cells (half-life 2.89 ± 1.37 h). The presence of cyclosporine A did not affect the uptake of RH123 but markedly increased the fluorescence intensity of RH123 in the liver and was associated with a slower elimination of RH123 from the liver (half-life 11.5 ± 4.24 h). In conclusion, the spatial disposition of RH123 in the rat liver and the effects of a transporter inhibitor on its disposition have been monitored over time using intravital imaging.","PeriodicalId":14512,"journal":{"name":"IntraVital","volume":"14 1","pages":"54 - 59"},"PeriodicalIF":0.0,"publicationDate":"2012-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"83519513","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 17

DISC 阀瓣

IntraVital Pub Date : 2012-07-01 DOI: 10.4161/intv.21896

H. Moreau, P. Bousso

引用次数: 5

Reconstitution of in vivo macrophage-tumor cell pairing and streaming motility on one-dimensional micro-patterned substrates. 巨噬细胞-肿瘤细胞在一维微图案基质上的配对和流动运动的重建。

IntraVital Pub Date : 2012-07-01 DOI: 10.4161/intv.22054

Ved P Sharma, Brian T Beaty, Antonia Patsialou, Huiping Liu, Michael Clarke, Dianne Cox, John S Condeelis, Robert J Eddy

{"title":"Reconstitution of in vivo macrophage-tumor cell pairing and streaming motility on one-dimensional micro-patterned substrates.","authors":"Ved P Sharma, Brian T Beaty, Antonia Patsialou, Huiping Liu, Michael Clarke, Dianne Cox, John S Condeelis, Robert J Eddy","doi":"10.4161/intv.22054","DOIUrl":"https://doi.org/10.4161/intv.22054","url":null,"abstract":"In mammary tumors, intravital imaging techniques have uncovered an essential role for macrophages during tumor cell invasion and metastasis mediated by an epidermal growth factor (EGF) / colony stimulating factor-1 (CSF-1) paracrine loop. It was previously demonstrated that mammary tumors in mice derived from rat carcinoma cells (MTLn3) exhibited high velocity migration on extracellular matrix (ECM) fibers. These cells form paracrine loop-dependent linear assemblies of alternating host macrophages and tumor cells known as \"streams.\" Here, we confirm by intravital imaging that similar streams form in close association with ECM fibers in a highly metastatic patient-derived orthotopic mammary tumor (TN1). To understand the in vivo cell motility behaviors observed in streams, an in vitro model of fibrillar tumor ECM utilizing adhesive 1D micropatterned substrates was developed. MTLn3 cells on 1D fibronectin or type I collagen substrates migrated with higher velocity than on 2D substrates and displayed enhanced lamellipodial protrusion and increased motility upon local interaction and pairing with bone marrow-derived macrophages (BMMs). Inhibitors of EGF or CSF-1 signaling disrupted this interaction and reduced tumor cell velocity and protrusion, validating the requirement for an intact paracrine loop. Both TN1 and MTLn3 cells in the presence of BMMs were capable of co-assembling into linear arrays of alternating tumor cells and BMMs that resembled streams in vivo, suggesting the stream assembly is cell autonomous and can be reconstituted on 1D substrates. Our results validate the use of 1D micropatterned substrates as a simple and defined approach to study fibrillar ECM-dependent cell pairing, migration and relay chemotaxis as a complementary tool to intravital imaging.","PeriodicalId":14512,"journal":{"name":"IntraVital","volume":"1 1","pages":"77-85"},"PeriodicalIF":0.0,"publicationDate":"2012-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.4161/intv.22054","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"32181674","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 47

Quantitative intravital microscopy of hepatic transport 肝脏运输的定量活体显微镜观察

IntraVital Pub Date : 2012-07-01 DOI: 10.4161/intv.21296

Clifford M. Babbey, Jennifer C Ryan, E. Gill, M. Ghabril, Courtney R. Burch, April Paulman, K. Dunn

引用次数: 21

Welcome to IntraVital 欢迎来到vitital

IntraVital Pub Date : 2012-07-01 DOI: 10.4161/INTV.21695

R. Weigert

引用次数: 4

Intravital imaging of cell signaling in mice 小鼠细胞信号的活体成像

IntraVital Pub Date : 2012-07-01 DOI: 10.4161/intv.20802

L. Ritsma, B. Ponsioen, J. van Rheenen

引用次数: 36

Nondestructive, serial in vivo imaging of a tissue-flap using a tissue adhesion barrier 利用组织粘连屏障对组织瓣进行非破坏性、连续的活体成像

IntraVital Pub Date : 2012-07-01 DOI: 10.4161/intv.21769

M. Kotsuma, N. Parashurama, B. Smith, John G. Wo, Ken Ito, S. Gambhir

{"title":"Nondestructive, serial in vivo imaging of a tissue-flap using a tissue adhesion barrier","authors":"M. Kotsuma, N. Parashurama, B. Smith, John G. Wo, Ken Ito, S. Gambhir","doi":"10.4161/intv.21769","DOIUrl":"https://doi.org/10.4161/intv.21769","url":null,"abstract":"Intravital Microscopy (IVM) is a powerful tool for imaging of dynamic events in living subjects and benefits from flexibility of various tissue preparation techniques. For example, a “tissue flap” (TF) approach initially affords high spatial resolution and physiological imaging with minimal tissue preparation, but serial TF imaging greatly increases the effects of pathological inflammation, resulting in postoperative adhesions and tissue injury. We took a materials science approach by implanting a commercially available, thin film, biopolymer tissue adhesion barrier (TAB) beneath the TF during serial imaging of the normal and developing breast in transgenic fluorescent mice, and with a fluorescent orthotopic mouse lymphoma model. We applied the TAB post-operatively beneath the TF to isolate the TF from the underlying peritoneum. When re-imaging the TF every 3–4 d, with a new TAB placed each time, we observed reduced hemorrhage, fibrous connective tissue and soft tissue damage. The presence of the TAB enabled sequential imaging of orthopically located EGFP+-lymphoma cells and associated vasculature at short intervals. In particular, it enabled visualization and tracking of the same individual fluorescent branches of the mammary gland in both adult and developing mice over time; likewise, it enabled tracking of lymph nodes. We conclude that this simple method affords great potential to serially track rare, microscopic, tissue-wide events in parenchyma or stroma. Potential applications include tracking proliferation and motility of transplanted cancer cells, stem cell-driven tissue growth, and tumor cell-stromal cell interactions at high spatial and temporal resolution.","PeriodicalId":14512,"journal":{"name":"IntraVital","volume":"20 1","pages":"69 - 76"},"PeriodicalIF":0.0,"publicationDate":"2012-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"88055187","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 9

In vivo dynamics of innate immune sentinels in the CNS. 中枢神经系统中先天性免疫哨兵的体内动态。

IntraVital Pub Date : 2012-01-01 DOI: 10.4161/intv.22823

Debasis Nayak, Bernd H Zinselmeyer, Kara N Corps, Dorian B McGavern

{"title":"In vivo dynamics of innate immune sentinels in the CNS.","authors":"Debasis Nayak, Bernd H Zinselmeyer, Kara N Corps, Dorian B McGavern","doi":"10.4161/intv.22823","DOIUrl":"10.4161/intv.22823","url":null,"abstract":"The innate immune system is comprised of cellular sentinels that often serve as the first responders to injury and invading pathogens. Our basic understanding of innate immunity is derived from research conducted in peripheral lymphoid tissues. However, it is now recognized that most non-lymphoid tissues throughout the body are equipped with specialized innate immune cells that are uniquely adapted to the niches in which they reside. The central nervous system (CNS) is a particularly interesting compartment because it contains a population of post-mitotic cells (neurons) that are intolerant of robust, cytopathic inflammatory responses observed in many peripheral tissues. Thus, evolutionary adaptations have fitted the CNS with a unique array of innate immune sentinels that facilitate the development of local inflammatory responses but attempt to do so in a manner that preserves the integrity of its post-mitotic residents. Interestingly, studies have even suggested that CNS resident innate immune cells contribute to the homeostasis of this compartment and promote neural activity. In this review we discuss recent advances in our understanding of CNS innate immune sentinels and how novel imaging approaches such as intravital two-photon laser scanning microscopy (TPLSM) have shed light on these cells during states of health and disease. ","PeriodicalId":14512,"journal":{"name":"IntraVital","volume":"1 2","pages":"95-106"},"PeriodicalIF":0.0,"publicationDate":"2012-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3784260/pdf/nihms491416.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"31769344","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0