DISC

Abstract

Dynamic in situ cytometry (DISC) is a new approach that combines advantages of both intravital imaging and flow cytometry analysis. The DISC methodology provides the opportunity to link cell behavior and phenotype in vivo and hence to perform cytometry in vivo and in real-time. In addition it enables you to readily display and analyze multiparametric imaging data. Through concrete examples, we present a guide to perform DISC experiments with the help of the custom designed DISCit software.