Iranian Biomedical Journal最新文献_第4页

Genetically Engineered Probiotics: Design, Therapeutics, and Clinical Translation. 基因工程益生菌：设计、治疗和临床翻译。

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Iranian Biomedical Journal Pub Date : 2025-11-01 DOI: 10.61882/ibj.5197

Mahsa Boogari, Maryam Mohebbi, Naghmeh Hadidi

{"title":"Genetically Engineered Probiotics: Design, Therapeutics, and Clinical Translation.","authors":"Mahsa Boogari, Maryam Mohebbi, Naghmeh Hadidi","doi":"10.61882/ibj.5197","DOIUrl":"10.61882/ibj.5197","url":null,"abstract":"Genetically engineered probiotics (GEPs) aim to address transient colonization and the intra- and inter-subject variability that limit conventional probiotics. These strains utilize Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR)/Cas editing, programmable gene circuits, and biosensors in chassis such as E. coli Nissle 1917 and L. lactis. This narrative review summarizes the current engineering toolkits and standards (e.g., SEVA), chassis selection criteria, biocontainment strategies, and translational requirements under CMC/GMP frameworks and discusses regulatory considerations for clinical translation. Representative examples include IL-10-secreting Lactococcus lactis and phenylalanine-metabolizing strains for phenylketonuria (SYNB1618/SYNB1934), which illustrate pharmacodynamic target engagement and short-term preclinical safety. We outline clinical advancements in predefined pharmacodynamics, durability of function, monitoring shedding and horizontal gene transfer, and genomic-microbiome-informed patient stratification. Systems modeling approaches (Genome-Scale Metabolic Model/ Agent-Based Model) are discussed as tools to guide rational design. GEPs offer programmable “sense-and-respond” therapeutics, with successful clinical adoption depending on durable efficacy, long-term safety, and clearly defined regulatory pathways.","PeriodicalId":14500,"journal":{"name":"Iranian Biomedical Journal","volume":"29 6","pages":"374-83"},"PeriodicalIF":1.9,"publicationDate":"2025-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13330702/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145900287","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Engineering a Potent Anti-MRSA Agent: The Development and Characterization of Chimeric Endolysin ZAM-MSC. 设计一种有效的抗mrsa药物：嵌合内溶素ZAM-MSC的开发和表征。

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Iranian Biomedical Journal Pub Date : 2025-11-01 DOI: 10.61882/ibj.5200

Atefeh Noori, Zahra Amini-Bayat, Saeed Mirdamadi, Farzaneh Azizmohseni, Seyed Shahriar Arab, Mahtab Moshref-Javadi

{"title":"Engineering a Potent Anti-MRSA Agent: The Development and Characterization of Chimeric Endolysin ZAM-MSC.","authors":"Atefeh Noori, Zahra Amini-Bayat, Saeed Mirdamadi, Farzaneh Azizmohseni, Seyed Shahriar Arab, Mahtab Moshref-Javadi","doi":"10.61882/ibj.5200","DOIUrl":"10.61882/ibj.5200","url":null,"abstract":"Background: The increasing prevalence of S. aureus, especially methicillin-resistant strains, poses a major healthcare threat due to limited therapies. To address this challenge, we engineered the chimeric endolysin ZAM-MSC as a potent antibiotic alternative, using domain-fusion strategies to enhance antibacterial activity. We designed ZAM-MSC by integrating the catalytic (M23) and cell wall-binding (SH3b) domains of lysostaphin with the catalytic domain (CHAP) from endolysin SAL-1. Structural optimization was performed using AlphaFold2 prediction, AutoDock Vina docking, and GROMACS simulations to evaluate domain interactions, protein stability, and binding dynamics.Methods: The chimeric construct was cloned into pCold I, expressed in E. coli, and purified under solubility-optimized conditions. Purified ZAM-MSC, at a minimum concentration of 3 μg, reduced bacterial optical density within 15 minutes, demonstrating potent lytic activity. Thermal stability assays indicated that ZAM-MSC retained its enzymatic activity over 80-90% across 4-37 °C, with only a 10-20% decrease at 25-37 °C after 30 minutes. NaCl stability tests revealed maximal activity in the absence of NaCl, with gradual reduction in enzyme activity by increasing NaCl concentrations.Results: Cytotoxicity analysis via MTT assay on L929 fibroblast cells showed cell viabilities of ~85-90% ± 5% at the highest enzyme concentrations tested, with no detectable cytotoxic effect compared to untreated controls. Hemolysis assays confirmed nearly 100% red blood cell integrity across all tested enzyme concentrations, supporting its biocompatibility with mammalian cells.Conclusion: Our findings establish ZAM-MSC as a highly promising therapeutic candidate, combining computational precision with robust experimental validation.","PeriodicalId":14500,"journal":{"name":"Iranian Biomedical Journal","volume":"29 6","pages":"384-96"},"PeriodicalIF":1.9,"publicationDate":"2025-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13332697/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146063522","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Helicobacter pylori Dihydroorotate Dehydrogenase: a Promising Target for Screening Potential Drug Candidates. 幽门螺杆菌二氢酸脱氢酶：筛选潜在候选药物的有希望的靶标。

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Iranian Biomedical Journal Pub Date : 2025-09-01 DOI: 10.61882/ibj.5231

Saba Ghaffari, Maryam Esmaeili, Fatemeh Ayoubnejad, Yeganeh Talebkhan, Marjan Mohammadi

{"title":"Helicobacter pylori Dihydroorotate Dehydrogenase: a Promising Target for Screening Potential Drug Candidates.","authors":"Saba Ghaffari, Maryam Esmaeili, Fatemeh Ayoubnejad, Yeganeh Talebkhan, Marjan Mohammadi","doi":"10.61882/ibj.5231","DOIUrl":"10.61882/ibj.5231","url":null,"abstract":"Background: De novo pyrimidine biosynthesis is essential for the survival of all living organisms. DHODH catalyzes the fourth step in this pathway. Inhibition of DHODH induces pyrimidine depletion and effectively eradicates microorganisms like H. pylori, which lacks the pyrimidine salvage pathway. Herein, we expressed rHp-DHODH, characterized its enzymatic activity, kinetics, and stability, and subsequently evaluated its inhibition by HQNO.Methods: The gene fragment encoding the rHp-DHODH protein was synthesized, subcloned, and expressed in soluble form. The recombinant protein was purified, its identity was confirmed, and its activity was measured using a colorimetric reduction assay. Kinetic parameters and the effects of pH, temperature, and incubation time on the enzymatic activity were investigated. The inhibitory effect of HQNO on rHp-DHODH was evaluated using the DCIP reduction assay. The MIC of HQNO against H. pylori was determined, and its bactericidal/bacteriostatic effect was assessed.Results: Optimal soluble expression of rHp-DHODH was achieved in BL21(DE3)pLysS. The enzyme exhibited a specific activity of 5.7 U/mg. Maximum activity was observed at pH 8.0 in Tris-HCl buffer at 25 °C. The Km values were 39.75 µM for DHO and 5.37 µM for CoQ10, with a kcat of 3.82 s-¹. The IC50 of HQNO against the recombinant enzyme was determined as 1.75 μM. HQNO inhibited H. pylori growth with an MIC of 0.5-1.0 µg/mL, displaying concentration-dependent bacteriostatic to bactericidal effects.Conclusions: The rHp-DHODH and its optimized enzymatic assay provide a reliable platform for screening candidate inhibitors, such as HQNO, advancing drug development efforts against H. pylori infection.","PeriodicalId":14500,"journal":{"name":"Iranian Biomedical Journal","volume":"29 5","pages":"309-20"},"PeriodicalIF":1.9,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13310527/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145482285","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Persian Medicinal Plants as Antidiabetic Agents: Mechanisms and Evidence from Bench to Bedside . 波斯药用植物作为抗糖尿病药物：从实验到临床的机制和证据。

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Iranian Biomedical Journal Pub Date : 2025-09-01 DOI: 10.61882/ibj.5073

Hasan Fallah Huseini, Masoud Haghighi, Saeed Kianbakht, Mojtaba Ziaee

{"title":"Persian Medicinal Plants as Antidiabetic Agents: Mechanisms and Evidence from Bench to Bedside .","authors":"Hasan Fallah Huseini, Masoud Haghighi, Saeed Kianbakht, Mojtaba Ziaee","doi":"10.61882/ibj.5073","DOIUrl":"10.61882/ibj.5073","url":null,"abstract":"The use of medicinal plants by diabetic patients dates back to ancient times. In recent years, numerous reports have been published on the efficacy and safety of many medicinal plants in the treatment of diabetes through various mechanisms. This review highlights the up-to-date proposed mechanisms of action of the most common antidiabetic herbs used in Persian medicine, comprising Cinnamomum zeylanicum, Trigonella foenum-graecum, Urtica dioica, Nigella sativa, Citrullus colocynthis, Silybum marianum, Zingiber officinale, Punica granatum, Salvia officinalis, Vaccinium arctostaphylos, and Momordica charantia, with support from clinical and experimental studies. Clinical research has shown significant reductions in blood glucose levels and insulin resistance, as well as improvements in diabetes-related symptoms, including digestive disorders and lipid dysregulation, accompanied by negligible adverse effects. Continuing to study how these plants work and how effectively they treat diabetes is important for using these natural treatments in modern medicine, offering affordable and safe options for diabetes patients.","PeriodicalId":14500,"journal":{"name":"Iranian Biomedical Journal","volume":"29 5","pages":"279-93"},"PeriodicalIF":1.9,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13311365/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145596505","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Chloroquine Enhances Mda-7- Induced Apoptosis via miR-7 and HSP70 Modulation in Glioblastoma. 氯喹通过miR-7和HSP70调节增强Mda-7诱导的胶质母细胞瘤细胞凋亡。

IF 1.9

Iranian Biomedical Journal Pub Date : 2025-09-01 DOI: 10.61882/ibj.5131

Seyedeh Maliheh Babazadeh, Mohammad Reza Zolfaghari, Mohsen Zargar, Kazem Baesi, Amir Ghaemi

{"title":"Chloroquine Enhances Mda-7- Induced Apoptosis via miR-7 and HSP70 Modulation in Glioblastoma.","authors":"Seyedeh Maliheh Babazadeh, Mohammad Reza Zolfaghari, Mohsen Zargar, Kazem Baesi, Amir Ghaemi","doi":"10.61882/ibj.5131","DOIUrl":"10.61882/ibj.5131","url":null,"abstract":"Background: Melanoma differentiation-associated gene-7 (Mda-7) selectively suppresses growth and induces apoptosis in various tumor cells without harming normal cells. Inhibition of autophagy has been shown to enhance the efficacy of many cancer therapies. However, its effect on the anticancer activity of Ad/Mda-7 in GBM has remained unclear. This study investigated the combined effect of an autophagy inhibitor (CQ) and Mda-7 in U87 cancer cells.Methods: Cell proliferation was assessed using the MTT assay. Apoptosis rates, autophagy induction, and ROS levels were measured using flow cytometry. Caspase-9 and β-actin protein levels were analyzed by Western blotting. ELISA was employed to quantify HSP70 and TRAIL level in the culture medium. Real-time PCR evaluated the expression levels of cell death-related genes (P38 MAPK, Bax, and TRAIL) and specific miRNAs (miR-7, miR-122, and miR-21) in treated cells.Results: Combined treatment with Ad/Mda-7 and the autophagy inhibitor CQ significantly reduced cell viability and proliferation. Ad/Mda-7 induced LC3-II protein expression in U87 cancer cells, which was further increased by autophagy inhibition through CQ. The combination treatment also increased apoptosis rates, elevated ROS levels, and decreased HSP70 protein expression, highlighting its synergistic anticancer effects. Increasing the expression of miR-7 and miR-122 indicated that the elevated levels of these endogenous miRNAs may help improve the treatment process.Conclusion: Our findings indicate that the combination of Ad/Mda-7 and CQ synergistically could inhibit U87 cancer cell growth and could serve as a promising approach for treating human GBM.","PeriodicalId":14500,"journal":{"name":"Iranian Biomedical Journal","volume":"29 5","pages":"294-308"},"PeriodicalIF":1.9,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13310548/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145482243","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Genome-Scale Metabolic Modeling Identifies Synergistic Metabolites that Enhance 5-Fluorouracil Efficacy in Colon Cancer. 基因组尺度代谢模型鉴定协同代谢物，增强5-氟尿嘧啶在结肠癌中的疗效。

IF 1.9

Iranian Biomedical Journal Pub Date : 2025-09-01 DOI: 10.61882/ibj.5178

Hasan Rahimi-Tamandegani, Ayed-Amir Marashi, Ghazaleh Ghavami, Mahya Mehrmohamadi, Soroush Sardari

{"title":"Genome-Scale Metabolic Modeling Identifies Synergistic Metabolites that Enhance 5-Fluorouracil Efficacy in Colon Cancer.","authors":"Hasan Rahimi-Tamandegani, Ayed-Amir Marashi, Ghazaleh Ghavami, Mahya Mehrmohamadi, Soroush Sardari","doi":"10.61882/ibj.5178","DOIUrl":"10.61882/ibj.5178","url":null,"abstract":"Background: Colon cancer remains a leading cause of cancer-related mortality, with the efficacy of standard chemotherapy agents such as 5-FU limited by resistance and toxicity. This study aimed to identify metabolites that enhance 5-FU efficacy in CRC using GEM.Methods: GEM was applied using the FVSEOF algorithm to identify metabolites that enhance the therapeutic efficacy of 5-FU in CRC. Context-specific metabolic models were constructed from TCGA data using the ftINIT algorithm. By simulating TS inhibition, we identified aspartate, lysine, and valine as candidate metabolites with altered uptake under constrained biomass production. Among them, lysine and valine are essential amino acids and were chosen for experimental validation using MTT assays and flow cytometry in HT-29 CRC cells and HU02 normal fibroblasts.Results: Our approach identified aspartate, lysine, and valine as candidate synergistic metabolites. Experimental validation confirmed the strong synergy of lysine and valine with 5-FU in HT-29 cells, while showing significantly reduced effects in normal fibroblasts. Mechanistic analysis suggested that these amino acids enhance nucleotide demand and metabolic activity, amplifying 5-FU-induced stress.Conclusion: This study demonstrates synergistic interventions and introduces amino acid co-supplementation as a potential strategy to improve CRC therapy with reduced toxicity. To our knowledge, this is the first study to employ GEM for the systematic prediction of metabolites that synergize with 5-FU, aiming to improve therapeutic outcomes in CRC.","PeriodicalId":14500,"journal":{"name":"Iranian Biomedical Journal","volume":"29 5","pages":"321-34"},"PeriodicalIF":1.9,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13310547/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145708336","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Neuroprotective Potential of Ocimum sanctum Polyphenols: Mechanisms and Therapeutic Implications. 茴香多酚的神经保护潜能：机制和治疗意义。

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Iranian Biomedical Journal Pub Date : 2025-09-01 DOI: 10.61882/ibj.5024

Nikhat Parween, Saurabh Pandey, Birendra Prasad

引用次数: 0

Effects of Cabergoline on Embryo Growth: Macroscopic and Molecular Findings. 卡麦角林对胚胎生长的影响：宏观和分子研究。

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Iranian Biomedical Journal Pub Date : 2025-09-01 DOI: 10.61882/ibj.4987

Gholamhossien Darya, Javad Moayedi, Zeinab Dehghan, Amin Derakhshanfar

{"title":"Effects of Cabergoline on Embryo Growth: Macroscopic and Molecular Findings.","authors":"Gholamhossien Darya, Javad Moayedi, Zeinab Dehghan, Amin Derakhshanfar","doi":"10.61882/ibj.4987","DOIUrl":"10.61882/ibj.4987","url":null,"abstract":"Background: Prolactin is a neurohypophyseal hormone that plays a major role in behavior, growth, and angiogenesis. CAB suppresses the effects of PRL by targeting the D2R. In this study, we used in silico and in vivo approaches to examine the side effects of CAB using chicken as a model.Methods: Thirty fertilized eggs were equally divided into three groups: one control and two experimental groups treated with CAB at doses of 0.5 mg/kg and 1 mg/kg, respectively. After 18 days of experiment, embryo weight, average body length, chorioallantoic vessels length, and relative PRL gene expression were analyzed. Also, prolactin-protein interaction and functional pathways regulated by PRL and associated proteins were investigated using the STRING database.Results: Macroscopic analysis showed a significant increase (17.54 ± 0.28) and decrease (12.92 ± 0.38) in weight in the 0.5 mg/kg and 1 mg/kg groups, respectively, as compared to the control. A significant reduction in body size and an increase in chorionic vessel development were observed only in the 0.5 mg/kg group. PRL expression levels significantly decreased in both CAB-treated groups. In silico analysis revealed PRL interactions with INS and POMC proteins, which are conserved between H. sapiens and G. gallus.Conclusion: Our findings indicate that CAB not only reduces prolactin levels but also affects fetal weight and body size.","PeriodicalId":14500,"journal":{"name":"Iranian Biomedical Journal","volume":"29 5","pages":"335-43"},"PeriodicalIF":1.9,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13310545/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145368033","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Reno-Protective Properties of Azolla pinnata Extract Against Gentamicin-Induced Kidney Damage Are Attributed to Its Antioxidant Effects. 凤仙花提取物抗庆大霉素所致肾损伤的抗氧化作用。

IF 1.9

Iranian Biomedical Journal Pub Date : 2025-09-01 DOI: 10.61882/ibj.5194

Mohammad Ghanbari Alamoti, Farideh Jalali Mashayekhi, Naser Hosseini, Houshang Najafi, Saeed Changizi-Ashtiyani

{"title":"Reno-Protective Properties of Azolla pinnata Extract Against Gentamicin-Induced Kidney Damage Are Attributed to Its Antioxidant Effects.","authors":"Mohammad Ghanbari Alamoti, Farideh Jalali Mashayekhi, Naser Hosseini, Houshang Najafi, Saeed Changizi-Ashtiyani","doi":"10.61882/ibj.5194","DOIUrl":"10.61882/ibj.5194","url":null,"abstract":"Background: Gentamicin, a powerful aminoglycoside antibiotic, is limited in clinical use due to dose-related kidney toxicity, mainly caused by oxidative stress. A. pinnata, an antioxidant-rich aquatic fern, has not been extensively studied for renoprotection against GM-induced kidney damage. This research assessed the protective effects of a hydroalcoholic extract of A. pinnata on GM nephrotoxicity.Methods: Forty male Wistar rats were divided into five groups (n = 8): control, sham, GM (100 mg/kg/day, i.p.), and GM plus A. pinnata extract (10 or 20 mg/kg/day, orally). After seven days, renal function markers (serum creatinine and urea), oxidative stress parameters (MDA, FRAP, CAT, and GPX), TNF-α, and renal histopathology were assessed.Results: GM significantly damaged kidney function and induced oxidative stress, as shown by increased levels of creatinine, urea, and MDA, along with reduced FRAP and CAT activity (p < 0.05). Co-treatment with A. pinnata extract, especially at 20 mg/kg, significantly lessened these effects by restoring kidney function markers, boosting antioxidant defenses, and lowering lipid peroxidation. The extract did not have a significant impact on either GPX activity or TNF-α levels. Histopathological analysis revealed that GM-induced tubular necrosis and glomerular damage were significantly ameliorated by A. pinnata in a dose-dependent manner.Conclusion: A. pinnata extract offers notable protection against kidney damage caused by gentamicin, mainly by enhancing the body's natural antioxidant defenses, decreasing lipid peroxidation, and maintaining the normal structure of kidney tissue. These findings suggest that A. pinnata could serve as a valuable complementary treatment to improve the safety of GM use.","PeriodicalId":14500,"journal":{"name":"Iranian Biomedical Journal","volume":"29 5","pages":"352-9"},"PeriodicalIF":1.9,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13310546/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145596581","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Alterations in the Expression of miR-148a-5p, TGF-β1, and TGF-βR2 in Skin Samples Exposed to Sulfur Mustard. 硫芥暴露皮肤样品中miR-148a-5p、TGF-β1和TGF-βR2表达的变化

IF 1.9

Iranian Biomedical Journal Pub Date : 2025-09-01 DOI: 10.61882/ibj.5198

Atiyeh Tavakoli, Saeed Heidari Keshel, Mohadese Valizadeh

{"title":"Alterations in the Expression of miR-148a-5p, TGF-β1, and TGF-βR2 in Skin Samples Exposed to Sulfur Mustard.","authors":"Atiyeh Tavakoli, Saeed Heidari Keshel, Mohadese Valizadeh","doi":"10.61882/ibj.5198","DOIUrl":"10.61882/ibj.5198","url":null,"abstract":"Background: Sulfur mustard exposure causes chronic cutaneous injuries characterized by inflammation, fibrosis, and delayed wound healing. MiRNAs, such as miR-148a-5p, have been implicated in regulating the TGF-β signaling pathways involved in these processes. This study aimed to evaluate whether alterations in the expression of miR-148a-5p, TGF-β1, and TGF-βR2 are associated with long-term SM-induced skin damage.Methods: Skin biopsy samples were collected from 20 SM-exposed veterans and 20 healthy controls. Total RNA was extracted, and quantitative real-time PCR was performed to assess the expression levels of miR-148a-5p, TGF-β1, and TGF-βR2. Group differences were analyzed using a t-test, and ROC curves were generated to evaluate diagnostic performance.Results: Expression levels of miR-148a-5p, TGF-β1, and TGF-βR2 were significantly lower in SM-exposed skin compared with controls (miR-148a-5p: p = 0.0010; TGF-β1: p < 0.0001; TGF-βR2: p < 0.0001). Based on ROC analysis, miR-148a-5p and TGF-βR2 indicated promising discriminative potential, whereas TGF-β1 did not reach statistical significance (AUC = 0.65; p = 0.0877).Conclusion: Our findings suggest that reduced expression of miR-148a-5p and TGF-βR2 may contribute to SM-related skin injury. Both markers demonstrated potential diagnostic utility and could aid in risk stratification and monitoring in SM-induced skin disease, pending further validation in larger cohorts.","PeriodicalId":14500,"journal":{"name":"Iranian Biomedical Journal","volume":"29 5","pages":"344-51"},"PeriodicalIF":1.9,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13310526/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145482287","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0