International Journal of Secondary Metabolite最新文献_第2页

Epigenetic factors of the effect of UV-C and X-ray presowing seeds radiation exposure in Matricaria chamomilla L. genotypes 紫外线-C 和 X 射线预播种子辐射对母菊基因型影响的表观遗传因素

International Journal of Secondary Metabolite Pub Date : 2024-04-22 DOI: 10.21448/ijsm.1358437

Daryna Sokolova, Alexandra Kravets, V. Zhuk, Ludmila Hlushchenko

{"title":"Epigenetic factors of the effect of UV-C and X-ray presowing seeds radiation exposure in Matricaria chamomilla L. genotypes","authors":"Daryna Sokolova, Alexandra Kravets, V. Zhuk, Ludmila Hlushchenko","doi":"10.21448/ijsm.1358437","DOIUrl":"https://doi.org/10.21448/ijsm.1358437","url":null,"abstract":"In a series of experiments using both X-ray and UV-C radiation exposure a parallel study of several pharmacological characteristics of the Matricaria chamomilla L. genotype group was carried out. The data concerning the changes in the productivity of pharmacological raw materials and stimulation of the synthesis of low molecular weight antioxidants as markers of secondary metabolism induction have been published earlier. In this study, the data on the relationship between the stimulation of the synthesis of secondary metabolites under different types of irradiation and the epigenetic changes in the plant organism are presented. It was shown that DNA methylation was switched to the de novo mode in plants of all studied genotypes of M. chamomilla under both types of irradiation. That indicates changes in the epigenetic program of the plant organism. Comparison of the epigenetic pattern between control and irradiated samples, based on the difference in DNA methylation patterns in terms of a statistical indicator, shows that there is no unambiguous relationship between the epigenetic changes and increasing yield of antioxidant synthesis. This is additional evidence of the diversity of metabolic rearrangements and adaptive strategies of the plant organism under radiation exposure even within one species.","PeriodicalId":14437,"journal":{"name":"International Journal of Secondary Metabolite","volume":"85 15","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-04-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140676969","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Phytochemical constituents of the roots of Heliotropium verdcourtii (Boraginaceae) Heliotropium verdcourtii（紫草科）根部的植物化学成分

International Journal of Secondary Metabolite Pub Date : 2024-04-22 DOI: 10.21448/ijsm.1301392

T. Tole, Habtamu Hailu Feso, L. Adane

{"title":"Phytochemical constituents of the roots of Heliotropium verdcourtii (Boraginaceae)","authors":"T. Tole, Habtamu Hailu Feso, L. Adane","doi":"10.21448/ijsm.1301392","DOIUrl":"https://doi.org/10.21448/ijsm.1301392","url":null,"abstract":"The medicinal value of medicinal plants lies in some bioactive constituents that produce a definite physiological action on the human body. Heliotropium verdcourtii is a deciduous shrub or small tree traditionally used in the treatment of various diseases including fever, dry cough, measles, convulsions, epilepsy, diarrhea, and other ailments. The chemical constituents of the roots of the plant were not investigated to date. The aim of the study was to investigate the phytochemicals present in the roots of Heliotropium verdcourtii. The freshly collected root of the plant was chopped and air dried under shade. The dried and finely grounded plant root was extracted through maceration with n-hexane, chloroform/methanol (v/v 1:1), and methanol successively. The extracts were subjected to qualitative phytochemical tests for screening the classes of secondary metabolites present in the plant. Compound isolation of the chloroform/methanol (v/v 1:1) extract was performed through silica gel chromatographic separation. The structures of all isolated compounds were determined by spectroscopic methods as well as comparison with previous reports in the literature. The yields of n-hexane, chloroform/methanol (v/v 1:1), and methanol extracts were 2.2 g (0.4%), 25 g (5.0%), and 19.8 g (4.0%), respectively. The qualitative phytochemical test of the extracts revealed the presence of flavonoids, terpenoids, phenolics, saponins, glycosides and alkaloids. Silica gel chromatographic separation afforded a mixture of three isomeric triterpenoids identified as α-amyrin, β-amyrin, and bauerenol. To the best of our knowledge these bioactive compounds were isolated from the root of this plant, for the first time.","PeriodicalId":14437,"journal":{"name":"International Journal of Secondary Metabolite","volume":"50 10","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-04-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140672946","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Optimization of factors affecting Agrobacterium-mediated hairy root induction in Vitex negundo L. (Lamiaceae) 农杆菌介导的毛细根诱导荆芥（灯心草科）的影响因素优化

International Journal of Secondary Metabolite Pub Date : 2024-04-22 DOI: 10.21448/ijsm.1368677

B. Mahakur, A. Moharana, S. K. Madkamı, Soumendra K. Naik, D. Barik

{"title":"Optimization of factors affecting Agrobacterium-mediated hairy root induction in Vitex negundo L. (Lamiaceae)","authors":"B. Mahakur, A. Moharana, S. K. Madkamı, Soumendra K. Naik, D. Barik","doi":"10.21448/ijsm.1368677","DOIUrl":"https://doi.org/10.21448/ijsm.1368677","url":null,"abstract":"Vitex negundo L. is an aromatic, woody, blooming shrub in the Verbenaceae family which can grow into a small tree. Traditionally, V. negundo root has been used to cure diabetes, colic, boils, leprosy, and rheumatism. Keeping the importance of its roots in mind, an attempt has been taken for development of a protocol for efficient hairy root proliferation system. The Agrobacterium rhizogenes strains (MTCC 532 and MTCC 2364) were used for hairy root induction. For A. rhizogenes infection, both in vitro and in vivo leaves as well as internodes were used as explant. In vitro leaves and internodal explants were obtained by the inoculation of matured nodal segments on the optimum medium [MS + 2.0 mg/L N6-Benzylaminopurine (BAP)] with c.a. 91.6% shoot regeneration and an average of 8.1 shoots per explants. In vitro leaf showed best hairy root induction followed by in vitro internode on ½ MS medium augmented with acetosyringone. Highest transformation efficiency was achieved using MTCC 2364 strain, while no transformation was observed in MTCC 532 strain. Different factors affecting transformation including co-cultivation period, infection time and optical density (O.D.) value were standardized. The highest efficacy, 88.8% hairy root induction was observed in in vitro leaves infected by MTCC 2364 for 60 minutes infection time with an O.D. value of 0.29 maintained over a 44-48 hours of co-cultivation period. The prescribed protocol may be used as a reference for development of industrial scale hairy root production for bioactive compound located in root of V. negundo.","PeriodicalId":14437,"journal":{"name":"International Journal of Secondary Metabolite","volume":"68 13","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-04-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140675825","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Investigating medicinal plants for antimicrobial benefits in a changing climate 在不断变化的气候中研究药用植物的抗菌功效

International Journal of Secondary Metabolite Pub Date : 2024-04-22 DOI: 10.21448/ijsm.1279531

Ali Yetgin

引用次数: 0

Antimicrobial, antioxidant and essential oil studies on Veratrum album L. (Melanthiaceae) 关于马鞭草（Melanthiaceae）的抗菌、抗氧化和精油研究

International Journal of Secondary Metabolite Pub Date : 2024-04-22 DOI: 10.21448/ijsm.1336752

Pelin YILMAZ SANCAR, Ş. İnci, A. Demirpolat, Sevda Kirbağ, Ş. Civelek

{"title":"Antimicrobial, antioxidant and essential oil studies on Veratrum album L. (Melanthiaceae)","authors":"Pelin YILMAZ SANCAR, Ş. İnci, A. Demirpolat, Sevda Kirbağ, Ş. Civelek","doi":"10.21448/ijsm.1336752","DOIUrl":"https://doi.org/10.21448/ijsm.1336752","url":null,"abstract":"In this study, essential oil components of the Veratrum album L. and the antimicrobial and antioxidant properties of these components were determined. The chemical composition of the essential oils of dried aerial parts of V. album was analyzed using GC and GC-MS. Antimicrobial activity was determined with the disk diffusion method. Total antioxidant status (TAS), total oxidant status (TOS) and 2.2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging capacity were detected for the antioxidant activity of the plant. According to the analysis results, the major essential oil components of the V. album were determined as hexacosane (39.5%), myristic (tetradecanoic) acid (22.8%), heptane (6.5%), anethole (4.9%) and 1,8- cineole (4.8%). The findings showed that the methanol extracts of the stem and leaf parts of the plant inhibited the growth of pathogenic microorganisms at different rates (14±0.1 - 34±0.3 mm). The TAS values of methanol extracts of stem and leaf parts of V. album were calculated as 3.75±0.07 and 3.91±0.01 mmol, while TOS values were calculated as 6.14±0.13 and 6.54±0.05 μmol. The scavenging activity of the DPPH radical increased depending on increasing concentrations of the plant extract.","PeriodicalId":14437,"journal":{"name":"International Journal of Secondary Metabolite","volume":"32 6","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-04-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140677429","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Investigation of Sterol structures and biological activities in Cochineal and Hibiscus sabdariffa extracts 苡仁和芙蓉提取物中的甾醇结构和生物活性研究

International Journal of Secondary Metabolite Pub Date : 2024-04-22 DOI: 10.21448/ijsm.1335924

Dilek Bahar, N. Kuşçulu, Mehmet Çadır

{"title":"Investigation of Sterol structures and biological activities in Cochineal and Hibiscus sabdariffa extracts","authors":"Dilek Bahar, N. Kuşçulu, Mehmet Çadır","doi":"10.21448/ijsm.1335924","DOIUrl":"https://doi.org/10.21448/ijsm.1335924","url":null,"abstract":"In the future, it is necessary to discover natural resources with no or less toxicity and side effects instead of synthetic drugs. Therefore, it is crucial to recognize, isolate, measure, and reveal the biological activities of substances in the structure of natural resources. In this study, the two extracts prepared from the plant (Hibiscus sabdariffa) and the insect (Dactylopius coccus) were examined. Sampling of the released substances was performed using Gas-Chromatography-Mass Spectrometry (GC-MS). In addition, viability, apoptosis, and oxidative stress of the derivatized samples were determined. Due to the biological differences between the samples, the chemical structures observed in the GC-MS analysis were not the same. It was observed that stigmasterol and its derivatives were found in the pomegranate sample, whereas naphthol and its derivatives were more abundant in the Cochineal insect sample. The cell viability increased with increasing concentrations of stigmasterol, which is present in large amounts in the structure of the pomegranate flower. However, the cell viability decreased with the Cochineal insect sample. Apoptosis and oxidative stress test results were also found to be different and contrasted for both samples. Therefore, the present study presents a new, natural, and different source that can be used as an antiviral and anticancer agent.","PeriodicalId":14437,"journal":{"name":"International Journal of Secondary Metabolite","volume":"51 18","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-04-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140675747","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

In-Vitro Bioactivity Evaluation of Hydrangenol Extracted from Hydrangea macrophylla (Thunb.) Ser. Leaves 从大绣球花（Thunb.）

International Journal of Secondary Metabolite Pub Date : 2024-01-06 DOI: 10.21448/ijsm.1390183

A. AL-YAFEAİ, Barbara Schmi̇tt, Angelika Malarski̇, Volker Böhm

{"title":"In-Vitro Bioactivity Evaluation of Hydrangenol Extracted from Hydrangea macrophylla (Thunb.) Ser. Leaves","authors":"A. AL-YAFEAİ, Barbara Schmi̇tt, Angelika Malarski̇, Volker Böhm","doi":"10.21448/ijsm.1390183","DOIUrl":"https://doi.org/10.21448/ijsm.1390183","url":null,"abstract":"Hydrangea macrophylla plant, native to Japan and Korea, has been attracting scientific attention due to its potential applications in both food science and health-related research. In this investigation, dry Hydrangea leaves were utilized as the source material. Subsequent to comminution and thermal treatment at 70 °C for an 18-hour duration, followed by a 30-minute ultrasonic bath extraction and a 5-minute centrifugation at 5000 rpm, hydrangenol was isolated through preparative HPLC. The investigation involved assessing the antioxidant capacity of hydrangenol, its impact on the activity of α-amylase and α-glucosidase enzymes, and its ability to prevent enzymatic browning. Quantification of antioxidant capacity, determined through TEAC (Trolox Equivalent Antioxidant Capacity), showed values from 1.8 to 3.2 mmol TE/mmol. Likewise, the ORAC (Oxygen Radical Absorbance Capacity) values were in the range of 16.5-27.0 mmol TE/mmol. Total phenolics content (Folin-Ciocalteu test) yielded a range of 7.1-11.2 g GAE (Gallic Acid Equivalents) per 100 g. Examining α-amylase inhibition, hydrangenol demonstrated a 52% inhibition (IC50: 3.6 mg/mL), whereas acarbose (positive control) displayed a higher inhibition of 99 % (IC50: 0.51 mg/mL). Regarding α-glucosidase inhibition, hydrangenol exhibited a 51% inhibition (IC50: 0.97 mg/mL), while acarbose displayed a 46% inhibition (IC50: 2.1 mg/mL). Additionally, the activity of PPO was suppressed by 61% at hydrangenol concentrations of 1 mg/mL and 2 mg/mL, and by 46% at a concentration of 4 mg/mL.","PeriodicalId":14437,"journal":{"name":"International Journal of Secondary Metabolite","volume":"79 2","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-01-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139629418","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Phytochemical study and biological activities of Teucrium mideltense (Batt.) Humbert. Teucrium mideltense (Batt.) Humbert 的植物化学研究和生物活性。

International Journal of Secondary Metabolite Pub Date : 2024-01-03 DOI: 10.21448/ijsm.1364491

H. Elbouny, B. Ouahzizi, Kaoutar Benrahou, A. Homrani Bakali, M. Bammou, K. Sellam, C. Alem

{"title":"Phytochemical study and biological activities of Teucrium mideltense (Batt.) Humbert.","authors":"H. Elbouny, B. Ouahzizi, Kaoutar Benrahou, A. Homrani Bakali, M. Bammou, K. Sellam, C. Alem","doi":"10.21448/ijsm.1364491","DOIUrl":"https://doi.org/10.21448/ijsm.1364491","url":null,"abstract":"Teucrium mideltense (Batt.) Humbert (T. mideltense) is an endemic Moroccan species that grows exclusively in the Oriental High Atlas Mountains of Morocco. In this work, we aim at determining the chemical profile and biological properties of the traditionally used aqueous extract of this plant. HPLC analysis, estimation of the amounts of total phenolic compounds including flavonoids, and in vitro antioxidant activity was evaluated according to the literature procedures (DPPH, ABTS, and FRAP). Additionally, safety assessment was carried out according to the organization for economic cooperation and development guidelines and the anti-hyperlipidemic activity was evaluated in triton-induced hyperlipidemic rat model. Our findings revealed that the aqueous extract of this plant contains significant amounts of phenolic compounds (91.94 mg GAE/gE) including flavonoids (27.41 mg RE/gE). HPLC analysis revealed the presence of vanillic acid, hesperidin, and rutin. Moreover, a considerable in vitro antioxidant effect was evaluated (DPPH IC50 = 36.10± 0.02 μg/mL; ABTS IC50 = 34.98± 1.31 μg/mL; FRAP EC50 = 129.74±2.18 μg/mL). Furthermore, T. mideltense (Batt.) Maire extract exerted significant lipid-lowering effects by reducing the levels of total cholesterol (-88.78%), triglycerides (-62.12%), and non-HDL cholesterol (-68.37%). We conclude that the supplementation with the aqueous extract of T. mideltense would be effective in lowering lipids under hyperlipidemic conditions.","PeriodicalId":14437,"journal":{"name":"International Journal of Secondary Metabolite","volume":"3 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-01-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139630838","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Effect of Na, Mg, Ca chloride salts on mineral element, proline and total protein contents in rice (Oryza sativa L.) grown in vitro 氯化钠、氯化镁、氯化钙盐对离体生长水稻（Oryza sativa L.）矿物质元素、脯氨酸和总蛋白质含量的影响

International Journal of Secondary Metabolite Pub Date : 2024-01-03 DOI: 10.21448/ijsm.1335099

Mehmet Yusuf Orcan, Pınar Orcan

{"title":"Effect of Na, Mg, Ca chloride salts on mineral element, proline and total protein contents in rice (Oryza sativa L.) grown in vitro","authors":"Mehmet Yusuf Orcan, Pınar Orcan","doi":"10.21448/ijsm.1335099","DOIUrl":"https://doi.org/10.21448/ijsm.1335099","url":null,"abstract":"In this study, the effects of different types and concentrations of salts on local Siverek rice plant (Oryza sativa L.) grown in vitro were investigated in terms of mineral elements (K, Ca, P, Mg, Na, Fe, Cu, Zn, Mn, Mo, Co), proline, and total protein content. Sterilized seeds were planted in hormone-free and salt-free MS medium. After one week, the seedlings were subjected to different concentrations of NaCl, CaCl2, and MgCl2 salts (0, 30 mM, 90 mM) in order to evaluate the effect of salinity on plant growth and development. In response to salt stress, a decrease in nutrient elements was observed for all three types of salt compared to the control group, which can be attributed to disruptions in ion balance. Changes in element levels generally showed varying levels of increase or decrease depending on both the type and concentration of the salt and these changes were statistically significant. The increase in proline level was found to be directly proportional to the changes in the amounts of Ca, Mg, K, and Na elements. Both total protein and proline content showed the lowest values for all salt concentrations with CaCl2, while the highest values were obtained with NaCl. In conclusion, the changes in the level of mineral elements, total protein, and proline content levels, which decrease or increase in different ratios, depending on the type and concentration rising of the salt, are associated with the varying tolerance of the plant to different types of salts.","PeriodicalId":14437,"journal":{"name":"International Journal of Secondary Metabolite","volume":"58 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-01-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140514343","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Bioactive compounds of Arctostaphylos uva-ursi wild-growing populations from Bulgaria 保加利亚野生欧鼠李的生物活性化合物

International Journal of Secondary Metabolite Pub Date : 2024-01-03 DOI: 10.21448/ijsm.1292525

M. Nikolova, I. Aneva, P. Zhelev, S. Berkov, E. YANKOVA-TSVETKOVA

{"title":"Bioactive compounds of Arctostaphylos uva-ursi wild-growing populations from Bulgaria","authors":"M. Nikolova, I. Aneva, P. Zhelev, S. Berkov, E. YANKOVA-TSVETKOVA","doi":"10.21448/ijsm.1292525","DOIUrl":"https://doi.org/10.21448/ijsm.1292525","url":null,"abstract":"Arctostaphylos uva-ursi (L.) Sprengel (bearberry), Ericaceae is a valuable medicinal plant with diuretic and uroantiseptic action which is mainly due to arbutin. In Bulgaria the species is considered as rare. Content assessment of bioactive compounds of A. uva-ursi leaves from three natural populations from Bulgaria was the aim of the present study. Leaf samples were collected from Pirin, Vitosha, and Rhodope Mountains. Bioactive compounds in the methanolic extracts of the samples were analysed by GC/MS. Total phenolic content was determined using Folin–Ciocalteu reagent. Arbutin, quinic acid and gallic acid were detected in the highest amounts. Catechine, 4-hydroxybenzoic acid, chlorogenic acid, triterpenes (α- and β-amyrin, uvaol and lupeol) and other primary and secondary metabolites were found, also. Differences in the content of individual compounds between samples of different origin were established. The highest total phenolic (182.98 mg GAE g-1) and arbutin (8.4%) content was found in the sample from Vitosha Mountain. The presented data characterizes the profile of bioactive compounds in the Bulgarian bearberry raw material for the first time.","PeriodicalId":14437,"journal":{"name":"International Journal of Secondary Metabolite","volume":"71 2","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-01-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139630571","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0