从大绣球花(Thunb.)

A. AL-YAFEAİ, Barbara Schmi̇tt, Angelika Malarski̇, Volker Böhm
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引用次数: 0

摘要

绣球花原产于日本和韩国,因其在食品科学和健康相关研究中的潜在应用而备受科学界关注。本研究以干燥的绣球花叶为原料。经过粉碎和 70 °C 18 小时的热处理,然后进行 30 分钟的超声波浴提取和 5 分钟的 5000 rpm 离心,最后通过制备型 HPLC 分离出水杨烯醇。调查包括评估水杨醇的抗氧化能力、对α-淀粉酶和α-葡萄糖苷酶活性的影响以及防止酶促褐变的能力。通过 TEAC(三氧化碳当量抗氧化能力)测定的抗氧化能力定量值为 1.8 至 3.2 毫摩尔 TE/毫摩尔。同样,ORAC(氧自由基吸收能力)值范围为 16.5-27.0 mmol TE/mmol。总酚含量(Folin-Ciocalteu 试验)为每 100 克 7.1-11.2 克 GAE(没食子酸当量)。在α-淀粉酶抑制作用方面,水飞蓟醇的抑制率为 52%(IC50:3.6 毫克/毫升),而阿卡波糖(阳性对照)的抑制率更高,为 99%(IC50:0.51 毫克/毫升)。在抑制α-葡萄糖苷酶方面,海兰根醇的抑制率为 51%(IC50:0.97 毫克/毫升),而阿卡波糖的抑制率为 46%(IC50:2.1 毫克/毫升)。此外,水飞蓟醇浓度为 1 毫克/毫升和 2 毫克/毫升时,PPO 的活性被抑制 61%,浓度为 4 毫克/毫升时被抑制 46%。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
In-Vitro Bioactivity Evaluation of Hydrangenol Extracted from Hydrangea macrophylla (Thunb.) Ser. Leaves
Hydrangea macrophylla plant, native to Japan and Korea, has been attracting scientific attention due to its potential applications in both food science and health-related research. In this investigation, dry Hydrangea leaves were utilized as the source material. Subsequent to comminution and thermal treatment at 70 °C for an 18-hour duration, followed by a 30-minute ultrasonic bath extraction and a 5-minute centrifugation at 5000 rpm, hydrangenol was isolated through preparative HPLC. The investigation involved assessing the antioxidant capacity of hydrangenol, its impact on the activity of α-amylase and α-glucosidase enzymes, and its ability to prevent enzymatic browning. Quantification of antioxidant capacity, determined through TEAC (Trolox Equivalent Antioxidant Capacity), showed values from 1.8 to 3.2 mmol TE/mmol. Likewise, the ORAC (Oxygen Radical Absorbance Capacity) values were in the range of 16.5-27.0 mmol TE/mmol. Total phenolics content (Folin-Ciocalteu test) yielded a range of 7.1-11.2 g GAE (Gallic Acid Equivalents) per 100 g. Examining α-amylase inhibition, hydrangenol demonstrated a 52% inhibition (IC50: 3.6 mg/mL), whereas acarbose (positive control) displayed a higher inhibition of 99 % (IC50: 0.51 mg/mL). Regarding α-glucosidase inhibition, hydrangenol exhibited a 51% inhibition (IC50: 0.97 mg/mL), while acarbose displayed a 46% inhibition (IC50: 2.1 mg/mL). Additionally, the activity of PPO was suppressed by 61% at hydrangenol concentrations of 1 mg/mL and 2 mg/mL, and by 46% at a concentration of 4 mg/mL.
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来源期刊
International Journal of Secondary Metabolite
International Journal of Secondary Metabolite Biochemistry, Genetics and Molecular Biology-Biotechnology
CiteScore
1.40
自引率
0.00%
发文量
28
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