International journal of systematic bacteriology最新文献_第8页

Pseudomonas libanensis sp. nov., a new species isolated from Lebanese spring waters. 黎巴嫩泉水假单胞菌一新种。

International journal of systematic bacteriology Pub Date : 1999-07-01 DOI: 10.1099/00207713-49-3-1091

F Dabboussi, M Hamze, M Elomari, S Verhille, N Baida, D Izard, H Leclerc

{"title":"Pseudomonas libanensis sp. nov., a new species isolated from Lebanese spring waters.","authors":"F Dabboussi, M Hamze, M Elomari, S Verhille, N Baida, D Izard, H Leclerc","doi":"10.1099/00207713-49-3-1091","DOIUrl":"https://doi.org/10.1099/00207713-49-3-1091","url":null,"abstract":"The taxonomic position of eight fluorescent Pseudomonas isolates, from two Lebanese spring waters, which were previously recognized by numerical analysis as members of a new subcluster (subcluster Vb) was examined. Except for one strain, the new subcluster exhibited internal DNA hybridization values of 76-100%, and 9-53% hybridization was measured with the type or reference strains of other Pseudomonas species. The highest DNA binding value was found with Pseudomonas marginalis strains (37-53%). The G+C content of the DNA of the type strain was 58 mol%. A comparison of 1322 nt of the 16S rRNA gene sequence of the strain representing subcluster Vb (CFML 96-195T) with the sequence of other strains of the genus Pseudomonas revealed that strain CFML 96-195T was part of the 'Pseudomonas fluorescens intrageneric cluster'. On the basis of the results of phenotypic, DNA-DNA and phylogenetic analyses, a new Pseudomonas species, Pseudomonas libanensis sp. nov., is proposed for the seven strains of subcluster Vb. The type strain is P. libanensis CFML 96-195T and has been deposited in the Collection de l'Institut Pasteur (Paris, France) as CIP 105460T. The P. libanensis strains are phenotypically and genotypically homogeneous and can be differentiated from most other fluorescent species by several phenotypic features. Differentiation of P. libanensis and Pseudomonas aeruginosa is based mainly on pyocyanin production; P. libanensis can be differentiated from P. fluorescens (all biovars) by alpha-aminobutyrate assimilation. The clinical significance of P. libanensis is unknown.","PeriodicalId":14428,"journal":{"name":"International journal of systematic bacteriology","volume":"49 Pt 3 ","pages":"1091-101"},"PeriodicalIF":0.0,"publicationDate":"1999-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1099/00207713-49-3-1091","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"21291191","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 48

Phylogenetic status of Anaerobacter polyendosporus, an anaerobic, polysporogenic bacterium. 厌氧多孢子菌厌氧多孢子菌的系统发育状况。

International journal of systematic bacteriology Pub Date : 1999-07-01 DOI: 10.1099/00207713-49-3-1119

A V Siunov, D V Nikitin, N E Suzina, V V Dmitriev, N P Kuzmin, V I Duda

{"title":"Phylogenetic status of Anaerobacter polyendosporus, an anaerobic, polysporogenic bacterium.","authors":"A V Siunov, D V Nikitin, N E Suzina, V V Dmitriev, N P Kuzmin, V I Duda","doi":"10.1099/00207713-49-3-1119","DOIUrl":"https://doi.org/10.1099/00207713-49-3-1119","url":null,"abstract":"The almost complete sequence of the 16S rRNA gene of the Gram-positive polysporogenic bacterium Anaerobacter polyendosporus was determined. This allowed phylogenetic analysis of A. polyendosporus by comparing sequences of the 16S rRNA gene of this bacterium to similar genes of other Gram-positive bacteria. It was shown that this polysporogenic bacterium belongs to the Clostridium cluster I, subcluster A. Phylogenetically, A. polyendosporus is distantly related to another polysporogenic, but non-cultivatable, bacterium, 'Metabacterium polyspora' and can be satisfactorily clustered within the saccharolytic clostridia with a low DNA G+C content grouped in subcluster A. A. polyendosporus was most closely related to Clostridium intestinale (94.8% identity of 16S rRNA genes) and Clostridium fallax (93.1%). Like other members of the Clostridium cluster I, subcluster A, A. polyendosporus possesses such common phenotypic features as a Gram-positive cell wall structure, anaerobiosis, derivation of energy from carbohydrate fermentation yielding butyric acid among other organic acids and the capacity for endogenous spore-formation. However, the scale of evolutionary change in the 16S rRNA gene between A. polyendosporus and phylogenetically related Clostridium species does not correspond to the profound changes in the phenotype of A. polyendosporus. Distinctive phenotypic features of the latter are large cell size, polysporogenesis (up to seven spores per cell), alternative modes of development and an unusual membrane ultrastructure.","PeriodicalId":14428,"journal":{"name":"International journal of systematic bacteriology","volume":"49 Pt 3 ","pages":"1119-24"},"PeriodicalIF":0.0,"publicationDate":"1999-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1099/00207713-49-3-1119","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"21291194","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 25

Comparative phylogeny of rrs and nifH genes in the Bacillaceae. 芽孢杆菌科rrs和nifH基因的系统发育比较。

International journal of systematic bacteriology Pub Date : 1999-07-01 DOI: 10.1099/00207713-49-3-961

W Achouak, P Normand, T Heulin

{"title":"Comparative phylogeny of rrs and nifH genes in the Bacillaceae.","authors":"W Achouak, P Normand, T Heulin","doi":"10.1099/00207713-49-3-961","DOIUrl":"https://doi.org/10.1099/00207713-49-3-961","url":null,"abstract":"The rrs (16S rDNA) gene sequences of nitrogen-fixing endospore-forming bacilli isolated from the rhizosphere of wheat and maize were determined in order to infer their phylogenetic position in the Bacillaceae. These rhizosphere strains form a monophyletic cluster with Paenibacillus azotofixans, Paenibacillus polymyxa and Paenibacillus macerans. Two of them (RSA19 and TOD45) had previously been identified as Bacillus circulans (group 2) by phenotypic characterization (API 50CH). Evidence for nitrogen fixation by P. azotofixans, P. polymyxa, P. macerans and putative B. circulans strains RSA19 and TOD45 was provided by acetylene-reduction activity, and confirmed by amplifying and sequencing a nifH fragment (370 nt). The phylogenetic tree of nifH-derived amino acid sequences was compared to the phylogenetic tree of rrs sequences. All Paenibacillus nifH sequences formed a coherent cluster distinct from that of related nitrogen-fixing anaerobic clostridia and Gram-positive high-G+C-content frankiae. The nifH gene was neither detected in the B. circulans type strain (ATCC 4513T) nor in the type strains of Bacillus subtilis, Bacillus cereus, Bacillus alcalophilus, Bacillus simplex, Brevibacillus brevis and Paenibacillus validus. Accordingly, nitrogen fixation among aerobic endospore-forming Firmicutes seems to be restricted to a subset of species in the genus Paenibacillus.","PeriodicalId":14428,"journal":{"name":"International journal of systematic bacteriology","volume":"49 Pt 3 ","pages":"961-7"},"PeriodicalIF":0.0,"publicationDate":"1999-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1099/00207713-49-3-961","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"21290731","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 82

Phylogenetic relationships of three amino-acid-utilizing anaerobes, Selenomonas acidaminovorans, 'Selenomonas acidaminophila' and Eubacterium acidaminophilum, as inferred from partial 16S rDNA nucleotide sequences and proposal of Thermanaerovibrio acidaminovorans gen. nov., comb. nov. and Anaeromusa acidaminophila gen. nov., comb. nov. 从部分16S rDNA核苷酸序列推断出三种氨基酸利用厌氧菌——酸氨基单胞菌、嗜酸氨基单胞菌和嗜酸氨基真杆菌的系统发育关系，并提出了嗜酸氨基热弧菌。11月和嗜酸厌氧藻11月，梳。11月。

International journal of systematic bacteriology Pub Date : 1999-07-01 DOI: 10.1099/00207713-49-3-969

S Baena, M L Fardeau, T H Woo, B Ollivier, M Labat, B K Patel

{"title":"Phylogenetic relationships of three amino-acid-utilizing anaerobes, Selenomonas acidaminovorans, 'Selenomonas acidaminophila' and Eubacterium acidaminophilum, as inferred from partial 16S rDNA nucleotide sequences and proposal of Thermanaerovibrio acidaminovorans gen. nov., comb. nov. and Anaeromusa acidaminophila gen. nov., comb. nov.","authors":"S Baena, M L Fardeau, T H Woo, B Ollivier, M Labat, B K Patel","doi":"10.1099/00207713-49-3-969","DOIUrl":"https://doi.org/10.1099/00207713-49-3-969","url":null,"abstract":"16S rRNA gene sequences of three previously described amino-acid-fermenting anaerobes, Selenomonas acidaminovorans, 'Selenomonas acidaminophila' and Eubacterium acidaminophilum, were determined. All three were found to cluster within the Clostridium and related genera of the subphylum of the Gram-positive bacteria. The thermophile, S. acidaminovorans, formed an individual line of descent and was equidistantly placed between Dethiosulfovibrio peptidovorans and Anaerobaculum thermoterrenum (similarity of 85%), both of which also form single lines of descent. 'S. acidaminophila' was related to Clostridium quercicolum, a member of cluster IX, with a similarity of 90%, whereas E. acidaminophilum was closely related to Clostridium litorale (similarity of 96%) as a member of cluster XI. Based on the phylogenetic data presented in this report and the phenotypic descriptions of these bacteria published previously, it is recommended that S. acidaminovorans be transferred to a new genus, Thermanaerovibrio gen. nov., as Thermanaerovibrio acidaminovorans comb. nov. and 'Selenomonas acidaminophila' be transferred to a new genus, Anaeromusa gen. nov., as Anaeromusa acidaminophila comb. nov. Though the transfer of E. acidaminophilum to a new taxon is justified, this is not recommended until the taxonomic status of all the members of cluster XI has been reviewed.","PeriodicalId":14428,"journal":{"name":"International journal of systematic bacteriology","volume":"49 Pt 3 ","pages":"969-74"},"PeriodicalIF":0.0,"publicationDate":"1999-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1099/00207713-49-3-969","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"21290732","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 63

Mycobacterium tuberculosis subsp. caprae subsp. nov.: a taxonomic study of a new member of the Mycobacterium tuberculosis complex isolated from goats in Spain. 结核分枝杆菌caprae无性系种群。11月:从西班牙山羊中分离的结核分枝杆菌复合体新成员的分类学研究。

International journal of systematic bacteriology Pub Date : 1999-07-01 DOI: 10.1099/00207713-49-3-1263

A Aranaz, E Liébana, E Gómez-Mampaso, J C Galán, D Cousins, A Ortega, J Blázquez, F Baquero, A Mateos, G Súarez, L Domínguez

{"title":"Mycobacterium tuberculosis subsp. caprae subsp. nov.: a taxonomic study of a new member of the Mycobacterium tuberculosis complex isolated from goats in Spain.","authors":"A Aranaz, E Liébana, E Gómez-Mampaso, J C Galán, D Cousins, A Ortega, J Blázquez, F Baquero, A Mateos, G Súarez, L Domínguez","doi":"10.1099/00207713-49-3-1263","DOIUrl":"https://doi.org/10.1099/00207713-49-3-1263","url":null,"abstract":"Isolates from the Mycobacterium tuberculosis complex cultured from caprine pathological tissue samples were biochemically and genetically characterized. The isolates were negative for nitrate reduction and niacin accumulation, they weakly hydrolysed Tween 80, were sensitive to pyrazinamide (50 micrograms ml-1) and were resistant to 1 and 2 micrograms tiophene-2-carboxylic acid hydrazide ml-1 but not to 5 or 10 micrograms tiophene-2-carboxylic acid hydrazide ml-1. Sequencing of the pncA gene revealed a polymorphism characteristic of M. tuberculosis, whereas oxyR, katG and gyrA sequences were characteristic of Mycobacterium bovis. The fingerprinting patterns obtained with IS6110, direct repeats and polymorphic G+C-rich sequence-associated RFLP and direct variable repeat-spacer oligonucelotide typing (spoligotyping) segregated these isolates from the other members of the complex. The results of this testing, together with the repeated association of this micro-organism with goats, suggest that a new member of this taxonomic complex not matching any of the classical species had been identified. This unusual mycobacterium may play a role in the epidemiology of animal and human tuberculosis in Spain. The name Mycobacterium tuberculosis subsp. caprae subsp. nov. is proposed for these isolates. The type strain of Mycobacterium tuberculosis subsp. caprae subsp. nov. is gM-1T (= CIP 105776T).","PeriodicalId":14428,"journal":{"name":"International journal of systematic bacteriology","volume":"49 Pt 3 ","pages":"1263-73"},"PeriodicalIF":0.0,"publicationDate":"1999-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1099/00207713-49-3-1263","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"21292205","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 205

rRNA gene RFLP as an identification tool for Corynebacterium species. rRNA基因RFLP作为棒状杆菌种类的鉴定工具。

International journal of systematic bacteriology Pub Date : 1999-07-01 DOI: 10.1099/00207713-49-3-983

J Björkroth, H Korkeala, G Funke

{"title":"rRNA gene RFLP as an identification tool for Corynebacterium species.","authors":"J Björkroth, H Korkeala, G Funke","doi":"10.1099/00207713-49-3-983","DOIUrl":"https://doi.org/10.1099/00207713-49-3-983","url":null,"abstract":"The value of rRNA gene RFLP analysis (ribotyping) as a tool for Corynebacterium and Turicella species identification was evaluated. Seventy-four strains representing 26 different species or subspecies were analysed by BstEII, SmaI and SphI ribotyping. Numerical analysis of the resulting rDNA banding patterns was performed by Dice coefficient correlation in order to establish a database for species identification. In general, most of the strains belonging to the same species clustered together. Interestingly, BstEII clustering of many species followed known phylogenetic lineages. This was not evident with the more heterogeneous SmaI and SphI patterns. The SmaI patterns contained a 1800 bp band in the digests of all species studied with the exception of Corynebacterium urealyticum. SphI digestion resulted in the most heterogeneous patterns. The information provided by all three enzymes was considered essential for the reliable linking of strains of unknown identity with defined species in the database. It is concluded that ribotyping provides an useful tool for screening and characterization of potentially new Corynebacterium species.","PeriodicalId":14428,"journal":{"name":"International journal of systematic bacteriology","volume":"49 Pt 3 ","pages":"983-9"},"PeriodicalIF":0.0,"publicationDate":"1999-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1099/00207713-49-3-983","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"21290734","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 18

Thauera mechernichensis sp. nov., an aerobic denitrifier from a leachate treatment plant. 来自渗滤液处理厂的好氧反硝化菌。

International journal of systematic bacteriology Pub Date : 1999-07-01 DOI: 10.1099/00207713-49-3-1045

E Scholten, T Lukow, G Auling, R M Kroppenstedt, F A Rainey, H Diekmann

引用次数: 134

Relationship of Bacillus subtilis clades associated with strains 168 and W23: a proposal for Bacillus subtilis subsp. subtilis subsp. nov. and Bacillus subtilis subsp. spizizenii subsp. nov.

International journal of systematic bacteriology Pub Date : 1999-07-01 DOI: 10.1099/00207713-49-3-1211

L K Nakamura, M S Roberts, F M Cohan

{"title":"Relationship of Bacillus subtilis clades associated with strains 168 and W23: a proposal for Bacillus subtilis subsp. subtilis subsp. nov. and Bacillus subtilis subsp. spizizenii subsp. nov.","authors":"L K Nakamura, M S Roberts, F M Cohan","doi":"10.1099/00207713-49-3-1211","DOIUrl":"https://doi.org/10.1099/00207713-49-3-1211","url":null,"abstract":"Earlier phylogenetic studies based on the inferred DNA sequences of the polC, rpoB and gyrA genes suggested that strains of the species Bacillus subtilis formed two clusters, indicating the presence two closely related taxa; one contained the laboratory strain 168 and the other the laboratory strain W23. Significant sexual isolation was found between strain 168 and members of the group containing W23, but no sexual isolation was observed between strain 168 and other members of the 168 group. DNA reassociation between the two groups ranged from 58 to 69% and intragroup DNA relatedness ranged from 82 to 100%. Because group 168 strains were highly related to the B. subtilis type strain, they were considered to be bona fide members of the species. About 99.5% sequence identity was observed between the 16S rRNA genes of the 168 and W23 groups. Ribitol and anhydroribitol were principal cell wall constituents of the W23 but not of the 168 group. These observations revealed two closely related but genetically and phenotypically distinct groups within B. subtilis that correspond to two historically important strains. Subspecies distinction is proposed for the 168 and W23 groups, with the names Bacillus subtilis subsp. subtilis subsp. nov. and Bacillus subtilis subsp. spizizenii subsp. nov., respectively. The type strain of the former is NRRL NRS-744T and the latter NRRL B-23049T.","PeriodicalId":14428,"journal":{"name":"International journal of systematic bacteriology","volume":"49 Pt 3 ","pages":"1211-5"},"PeriodicalIF":0.0,"publicationDate":"1999-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1099/00207713-49-3-1211","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"21292325","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 191

Proposal of Virgibacillus proomii sp. nov. and emended description of Virgibacillus pantothenticus (Proom and Knight 1950) Heyndrickx et al. 1998. propromii Virgibacillus sp. 11 .的提出和对泛酸Virgibacillus pantothenticus的修订描述(Proom and Knight 1950)。

International journal of systematic bacteriology Pub Date : 1999-07-01 DOI: 10.1099/00207713-49-3-1083

M Heyndrickx, L Lebbe, K Kersters, B Hoste, R De Wachter, P De Vos, G Forsyth, N A Logan

引用次数: 58

Taxonomic characterization of denitrifying bacteria that degrade aromatic compounds and description of Azoarcus toluvorans sp. nov. and Azoarcus toluclasticus sp. nov. 降解芳香族化合物的反硝化细菌的分类特征及甲粪偶氮菌和甲粪偶氮菌的描述。

International journal of systematic bacteriology Pub Date : 1999-07-01 DOI: 10.1099/00207713-49-3-1129

B Song, M M Häggblom, J Zhou, J M Tiedje, N J Palleroni

{"title":"Taxonomic characterization of denitrifying bacteria that degrade aromatic compounds and description of Azoarcus toluvorans sp. nov. and Azoarcus toluclasticus sp. nov.","authors":"B Song, M M Häggblom, J Zhou, J M Tiedje, N J Palleroni","doi":"10.1099/00207713-49-3-1129","DOIUrl":"https://doi.org/10.1099/00207713-49-3-1129","url":null,"abstract":"A taxonomic characterization of twenty-one strains capable of degrading aromatic compounds under denitrifying conditions, isolated from ten different geographical locations, was performed on the basis of general morphological and physiological characteristics, cellular fatty acids, DNA base composition, small ribosomal (16S) subunit DNA sequences, whole-cell protein patterns and genomic DNA fragmentation analysis, in addition to DNA similarity estimations using hybridization methods. The collection of strains was subdivided into a number of different groups. A first group, consisting of four strains, could be assigned to the previously described species Azoarcus tolulyticus. A second group (five strains) had DNA which reannealed highly to that of strains of the first group, and it is considered to represent a genomovar of A. tolulyticus. The third and fourth groups, composed of a total of five strains, represent a new species of Azoarcus, Azoarcus toluclasticus (group 3) and a genomovar of this species (group 4), respectively. Finally, the fifth group, with two strains, corresponds to another new species of the genus Azoarcus, Azoarcus toluvorans. In addition to these five groups, the collection includes five individual strains perhaps representing as many different new species. The above classification is partially consistent with the results of approaches other than DNA-DNA hybridization (electrophoretic patterns of whole-cell proteins and of the fragments obtained after digestion of total DNA with infrequently cutting restriction enzymes). On the other hand, no correlation of these groupings was found in terms of the cellular fatty acid composition. It is also unfortunate that no simple sets of easily determinable phenotypic properties could be defined as being characteristic of each of the groups.","PeriodicalId":14428,"journal":{"name":"International journal of systematic bacteriology","volume":"49 Pt 3 ","pages":"1129-40"},"PeriodicalIF":0.0,"publicationDate":"1999-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1099/00207713-49-3-1129","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"21291196","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 96