{"title":"Gordonia alkanivorans sp. nov., isolated from tar-contaminated soil.","authors":"C Kummer, P Schumann, E Stackebrandt","doi":"10.1099/00207713-49-4-1513","DOIUrl":"https://doi.org/10.1099/00207713-49-4-1513","url":null,"abstract":"<p><p>Twelve bacterial strains isolated from tar-contaminated soil were subjected to a polyphasic taxonomic study. The strains possessed meso-diaminopimelic acid as the diagnostic diamino acid of the peptidoglycan, MK-9(H2) as the predominant menaquinone, long-chain mycolic acids of the Gordonia-type, straight-chain saturated and monounsaturated fatty acids, and considerable amounts of tuberculostearic acid. The G + C content of the DNA was 68 mol%. Chemotaxonomic and physiological properties and 16S rDNA sequence comparison results indicated that these strains represent a new species of the genus Gordonia. Because of the ability of these strains to use alkanes as a carbon source, the name Gordonia alkanivorans is proposed. The type strain of Gordonia alkanivorans sp. nov. is strain HKI 0136T (= DSM 44369T).</p>","PeriodicalId":14428,"journal":{"name":"International journal of systematic bacteriology","volume":"49 Pt 4 ","pages":"1513-22"},"PeriodicalIF":0.0,"publicationDate":"1999-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1099/00207713-49-4-1513","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"21415625","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
M D Collins, M Rodriguez Jovita, G Foster, B Sjödén, E Falsen
{"title":"Characterization of a Gemella-like organism from the oral cavity of a dog: description of Gemella palaticanis sp. nov.","authors":"M D Collins, M Rodriguez Jovita, G Foster, B Sjödén, E Falsen","doi":"10.1099/00207713-49-4-1523","DOIUrl":"https://doi.org/10.1099/00207713-49-4-1523","url":null,"abstract":"<p><p>A hitherto unknown Gram-positive, catalase-negative, facultatively anaerobic coccus isolated from a vesicle on the gum of a dog was characterized by phenotypic and molecular taxonomic methods. Comparative 16S rRNA gene sequencing studies demonstrated that the isolate represents a new subline within the genus Gemella. The unknown bacterium was readily distinguished from all currently described members of this genus, Gemella haemolysans, Gemella bergeri, Gemella morbillorum and Gemella sanguinis, by biochemical tests and electrophoretic analysis of whole-cell proteins. On the basis of phylogenetic and phenotypic evidence, it is proposed that the unknown bacterium be classified as Gemella palaticanis sp. nov. The type strain of Gemella palaticanis is CCUG 39489T.</p>","PeriodicalId":14428,"journal":{"name":"International journal of systematic bacteriology","volume":"49 Pt 4 ","pages":"1523-6"},"PeriodicalIF":0.0,"publicationDate":"1999-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1099/00207713-49-4-1523","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"21415626","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
S B Kim, R Brown, C Oldfield, S C Gilbert, M Goodfellow
{"title":"Gordonia desulfuricans sp. nov., a benzothiophene-desulphurizing actinomycete.","authors":"S B Kim, R Brown, C Oldfield, S C Gilbert, M Goodfellow","doi":"10.1099/00207713-49-4-1845","DOIUrl":"https://doi.org/10.1099/00207713-49-4-1845","url":null,"abstract":"<p><p>The taxonomic position of two actinomycetes isolated from soil was established using a polyphasic approach. The organisms, designated 213ET and 213F, were found to have chemical and morphological properties consistent with their assignment to the genus Gordonia. Nearly complete sequences of the 16S rDNA genes of the two strains were determined following the isolation and direct sequencing of the amplified genes. The tested strains were found to have identical 16S rDNA sequences and formed a phylogenetic line within the evolutionary radiation occupied by the genus Gordonia that was most closely related to Gordonia rubropertincta DSM 43197T. However, DNA-DNA relatedness data showed that strain 213ET and Gordonia rubropertincta DSM 43197T belonged to distinct genomic species. Strains 213ET and 213F also shared an identical phenotypic profile which distinguished them from representatives of validly described Gordonia species. The combined genotypic and phenotypic data show that strains 213ET and 213F merit recognition as a new species of Gordonia. The name proposed for the new species is Gordonia desulfuricans, for which the type strain is 213ET (= NCIMB 40816T).</p>","PeriodicalId":14428,"journal":{"name":"International journal of systematic bacteriology","volume":"49 Pt 4 ","pages":"1845-51"},"PeriodicalIF":0.0,"publicationDate":"1999-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1099/00207713-49-4-1845","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"21415859","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
A Jöborn, M Dorsch, J C Olsson, A Westerdahl, S Kjelleberg
{"title":"Carnobacterium inhibens sp. nov., isolated from the intestine of Atlantic salmon (Salmo salar).","authors":"A Jöborn, M Dorsch, J C Olsson, A Westerdahl, S Kjelleberg","doi":"10.1099/00207713-49-4-1891","DOIUrl":"https://doi.org/10.1099/00207713-49-4-1891","url":null,"abstract":"<p><p>Strain K1T, isolated from the gastrointestinal tract of Atlantic salmon (Salmo salar), has the capacity to inhibit the growth of the fish pathogens Vibrio anguillarum and Aeromonas salmonicida. Strain K1T is a motile Gram-positive psychrophilic rod that lacks both catalase and oxidase, which does not grow on acetate containing media, but grows at pH 9 and in TSB with up to 6% sodium chloride content. Strain K1T is facultatively anaerobic and tryptone as a sole source of nutrient promotes growth. The most abundant cellular fatty acid of strain K1T is oleic acid (18:1cis9). Based on 16S rDNA sequence comparisons, it is suggested that strain K1T is phylogenetically closely related to C. alterfunditum. However, the unique phenotypic attributes of strain K1T suggest that it represents a new species. The name Carnobacterium inhibens is proposed, for which the type strain is K1T (= CCUG 31728T).</p>","PeriodicalId":14428,"journal":{"name":"International journal of systematic bacteriology","volume":"49 Pt 4 ","pages":"1891-8"},"PeriodicalIF":0.0,"publicationDate":"1999-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1099/00207713-49-4-1891","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"21415863","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
A Linos, A Steinbüchel, C Spröer, R M Kroppenstedt
{"title":"Gordonia polyisoprenivorans sp. nov., a rubber-degrading actinomycete isolated from an automobile tyre.","authors":"A Linos, A Steinbüchel, C Spröer, R M Kroppenstedt","doi":"10.1099/00207713-49-4-1785","DOIUrl":"https://doi.org/10.1099/00207713-49-4-1785","url":null,"abstract":"<p><p>A rubber-degrading bacterium (strain Kd2T) was isolated from fouling tyre water inside a deteriorated automobile tyre. The strain was aerobic, Grampositive, produced elementary branching hyphae which fragmented into rod/coccus-like elements and showed chemotaxonomic markers which were consistent with the classification of Gordonia, i.e. meso-diaminopimelic acid, N-glycolyl muramic acid, arabinose and galactose as diagnostic sugars, a fatty acid pattern composed of unbranched saturated and monounsaturated fatty acids with a considerable amount of tuberculostearic acid, and mycolic acids comprising 58-66 carbon atoms with two principal mycolic acids C60 and C62 counting for over 60%. Results of 16S rDNA analyses as well as chemotaxonomic results, led to the conclusion that Gordonia sp. strain Kd2T (= DSM 44302T) represents a new species within the genus Gordonia for which the name Gordonia polyisoprenivorans is proposed.</p>","PeriodicalId":14428,"journal":{"name":"International journal of systematic bacteriology","volume":"49 Pt 4 ","pages":"1785-91"},"PeriodicalIF":0.0,"publicationDate":"1999-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1099/00207713-49-4-1785","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"21415942","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Sporichthya brevicatena sp. nov.","authors":"T Tamura, M Hayakawa, K Hatano","doi":"10.1099/00207713-49-4-1779","DOIUrl":"https://doi.org/10.1099/00207713-49-4-1779","url":null,"abstract":"<p><p>A strain was isolated from soil that formed short chains of aerial mycelium dividing into motile spores on an agar medium, but not substrate mycelium. The organism had menaquinone 9(H8), LL-diaminopimelic acid in the cell wall amino acids and a DNA G + C content of 71 mol%. On the basis of the morphological and chemotaxonomic characteristics and phylogenetic analysis, this strain was classified into the genus Sporichthya. DNA-DNA hybridization revealed that the strain differed from the known species, Sporichthya polymorpha. Therefore, a new species, Sporichthya brevicatena sp. nov., is proposed. The type strain is S. brevicatena YU720-21T (= IFO 16195T).</p>","PeriodicalId":14428,"journal":{"name":"International journal of systematic bacteriology","volume":"49 Pt 4 ","pages":"1779-84"},"PeriodicalIF":0.0,"publicationDate":"1999-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1099/00207713-49-4-1779","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"21415941","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
M D Collins, M Rodriguez Jovita, P A Lawson, E Falsen, G Foster
{"title":"Characterization of a novel gram-positive, catalase-negative coccus from horses: description of Eremococcus coleocola gen. nov., sp. nov.","authors":"M D Collins, M Rodriguez Jovita, P A Lawson, E Falsen, G Foster","doi":"10.1099/00207713-49-4-1381","DOIUrl":"https://doi.org/10.1099/00207713-49-4-1381","url":null,"abstract":"<p><p>Two strains of an unknown Gram-positive, catalase-negative, facultatively anaerobic coccus originating from the reproductive tract of horses were characterized by phenotypic and molecular taxonomic methods. Comparative 16S rRNA gene sequencing studies demonstrated that the two strains constitute a new subline within the lactic-acid group of bacteria, close to, but distinct from, Abiotrophia defectiva, Globicatella sanguinis and close relatives. The unknown bacterium was readily distinguished from other described Gram-positive, catalase-negative cocci by biochemical tests and electrophoretic analysis of whole-cell proteins. On the basis of phylogenetic and phenotypic evidence, it is proposed that the unknown bacterium be classified as Eremococcus coleocola gen. nov., sp. nov. The type strain of Eremococcus coleocola is CCUG 38207T.</p>","PeriodicalId":14428,"journal":{"name":"International journal of systematic bacteriology","volume":"49 Pt 4 ","pages":"1381-5"},"PeriodicalIF":0.0,"publicationDate":"1999-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1099/00207713-49-4-1381","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"21415214","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
A M Maszenan, R J Seviour, B K Patel, P Schumann, J Burghardt, R I Webb, J A Soddell, G N Rees
{"title":"Friedmanniella spumicola sp. nov. and Friedmanniella capsulata sp. nov. from activated sludge foam: gram-positive cocci that grow in aggregates of repeating groups of cocci.","authors":"A M Maszenan, R J Seviour, B K Patel, P Schumann, J Burghardt, R I Webb, J A Soddell, G N Rees","doi":"10.1099/00207713-49-4-1667","DOIUrl":"https://doi.org/10.1099/00207713-49-4-1667","url":null,"abstract":"<p><p>Two Gram-positive, non-motile, non-spore-forming, strictly aerobic, pigmented cocci, strains Ben 107T and Ben 108T, growing in aggregates were isolated from activated sludge samples by micromanipulation. Both possessed the rare type A3 gamma' peptidoglycan. Major menaquinones of strain Ben 107T were MK-9(H4) and MK-7(H2), and the main cellular fatty acid was 12-methyltetradecanoic acid (ai-C15:0). In strain Ben 108T, MK-9(H4), MK-9(H2) and MK-7(H4) were the menaquinones and again the main fatty acid was 12-methyltetradecanoic acid (ai-C15:0). Polar lipids in both strains consisted of phosphatidyl inositol, phosphatidyl glycerol and diphosphatidyl glycerol with two other unidentified glycolipids and phospholipids also present in both. These data, together with the 16S rDNA sequence data, suggest that strain Ben 107T belongs to the genus Friedmanniella which presently includes a single recently described species, Friedmanniella antarctica. Although the taxonomic status of strain Ben 108T is far less certain, on the basis of its 16S rRNA sequence it is also adjudged to be best placed in the genus Friedmanniella. The chemotaxonomic characteristics and DNA-DNA hybridization data support the view that Ben 107T and Ben 108T are novel species of the genus Friedmanniella. Hence, it is proposed that strain Ben 107T (= ACM 5121T) is named as Friedmanniella spumicola sp. nov. and strain Ben 108T (= ACM 5120T) as Friedmanniella capsulata sp. nov.</p>","PeriodicalId":14428,"journal":{"name":"International journal of systematic bacteriology","volume":"49 Pt 4 ","pages":"1667-80"},"PeriodicalIF":0.0,"publicationDate":"1999-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1099/00207713-49-4-1667","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"21414932","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Janthinobacterium agaricidamnosum sp. nov., a soft rot pathogen of Agaricus bisporus.","authors":"S P Lincoln, T R Fermor, B J Tindall","doi":"10.1099/00207713-49-4-1577","DOIUrl":"https://doi.org/10.1099/00207713-49-4-1577","url":null,"abstract":"<p><p>A novel bacterium has been found that causes a soft rot disease of Agaricus bisporus, the cultivated mushroom. It has been characterized using nutritional, physiological, chemical and molecular techniques. Based on these data, it was shown to have many characteristics in common with members of the genus Janthinobacterium. Despite similarities to the only described species within this genus, Janthinobacterium lividum, there were a number of differences between the mushroom pathogen isolated and this species. Despite the high degree of genotypic similarity between members of the genus Janthinobacterium and Herbaspirillum, as evidenced by DNA-RNA hybridization, and the high degree of 16S rDNA sequence similarity between members of the genera Janthinobacterium, Herbaspirillum, Oxalobacter and Duganella, as well as the generically misnamed Pseudomonas lemoignei, it was possible to show that members of the genus Janthinobacterium could be easily distinguished from these taxa. The data also indicated that the mushroom pathogenic strains represent a novel species within the genus Janthinobacterium for which the name Janthinobacterium agaricidamnosum sp. nov. is proposed. The type strain of this species has been deposited in the Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH, Braunschweig, Germany, as DSM 9628T and at the National Collection of Plant-pathogenic bacteria, UK, as NCPPB 3945T. To aid practical control of the disease, the effect of the relative humidity on symptom expression on Agaricus bisporus was determined.</p>","PeriodicalId":14428,"journal":{"name":"International journal of systematic bacteriology","volume":"49 Pt 4 ","pages":"1577-89"},"PeriodicalIF":0.0,"publicationDate":"1999-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1099/00207713-49-4-1577","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"21415032","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"A novel species of thermoacidophilic archaeon, Sulfolobus yangmingensis sp. nov.","authors":"R L Jan, J Wu, S M Chaw, C W Tsai, S D Tsen","doi":"10.1099/00207713-49-4-1809","DOIUrl":"https://doi.org/10.1099/00207713-49-4-1809","url":null,"abstract":"<p><p>A novel microbe was isolated from a geothermal vent in Yang-Ming National Park in northern Taiwan. This spherical microbe with mean cell diameter of 1.1 +/- 0.2 microns is a facultatively chemolithoautotrophic archaeon that grows on elemental sulfur and reduced sulfur compounds. The optimal pH and temperature for growth are 4.0 (pH range 2.0-6.0) and 80 degrees C (temperature range 65-95 degrees C). Its membranes contain the lipids calditoglycerocaldarchaeol and caldarchaeol, which are common to other members of the Sulfolobaceae. Like Sulfolobus acidocaldarius, Sulfolobus shibatae and Sulfolobus solfataricus, the new isolate utilizes sugars and amino acids effectively as sole carbon sources. The G + C content of the genomic DNA was 42 mol%. DNA of the isolate hybridized weakly to the DNA of other Sulfolobus species. Phylogenetic analysis of the 16S rRNA indicated that the new isolate represents a deep branch within the genus Sulfolobus. On the basis of these properties, the new isolate appears to represent a new species of Sulfolobus, for which the name Sulfolobus yangmingensis sp. nov. is proposed. The type strain is strain YM1T.</p>","PeriodicalId":14428,"journal":{"name":"International journal of systematic bacteriology","volume":"49 Pt 4 ","pages":"1809-16"},"PeriodicalIF":0.0,"publicationDate":"1999-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1099/00207713-49-4-1809","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"21415945","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}