International Review of Biophysical Chemistry最新文献

Biocoagulants for Water and Waste Water Purification: a Review 生物混凝剂用于水和废水净化的研究进展

International Review of Biophysical Chemistry Pub Date : 2015-06-30 DOI: 10.15866/IREBIC.V5I3.5976

K. Yongabi

引用次数: 53

Optimisation of Poly(γ-Glutamic Acid) Production by Bacillus velezensis NRRL B – 23189 in Liquid Fermentation with Molasses as the Carbon Source without Addition of Glutamic Acid 以糖蜜为碳源、不添加谷氨酸的velezensis芽孢杆菌NRRL B - 23189液体发酵产聚γ-谷氨酸的优化

International Review of Biophysical Chemistry Pub Date : 2014-10-31 DOI: 10.15866/IREBIC.V5I5.5995

Luana Pereira de Moraes, R. Alegre, P. N. Brito

{"title":"Optimisation of Poly(γ-Glutamic Acid) Production by Bacillus velezensis NRRL B – 23189 in Liquid Fermentation with Molasses as the Carbon Source without Addition of Glutamic Acid","authors":"Luana Pereira de Moraes, R. Alegre, P. N. Brito","doi":"10.15866/IREBIC.V5I5.5995","DOIUrl":"https://doi.org/10.15866/IREBIC.V5I5.5995","url":null,"abstract":"Poly (γ-glutamic acid), also known as γ-PGA, is an extracellular polymer produced by microbial fermentation. It is water-soluble, edible, biodegradable, non-toxic towards humans and the environment, and it has many available sites for drug conjugation and a powerful ability to solubilise hydrophobic molecules. This work reports the application of molasses, citric acid and ammonium sulphate in the fermentation by Bacillus velezensis NRRL-23189 to produce γ-PGA and the detection of molasses consumption without the use of glutamic acid as a nutrient. Different concentrations of molasses, citric acid and ammonium sulphate were studied. The fermentation was agitated at 200 rpm at 27oC for 72 h, with an initial pH of 6.5 (NaOH 2N and HCl 2N). Spectrophotometric analyses were used to measure concentrations of γ-PGA and the residual sugar from molasses degradation. The maximum production of γ-PGA was 4.82 g/l, in a medium with molasses (200g/l), citric acid (12.5g/l) and ammonium sulphate (8g/l) in a fermentation that also resulted in the maximum sugar consumption.","PeriodicalId":14377,"journal":{"name":"International Review of Biophysical Chemistry","volume":"20 1","pages":"130-135"},"PeriodicalIF":0.0,"publicationDate":"2014-10-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"81916826","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 9

Effects of Transesterification Parameters on the Biodiesel Produced from Crude Groundnut Oil 花生油酯交换工艺参数对制备生物柴油的影响

International Review of Biophysical Chemistry Pub Date : 2014-10-31 DOI: 10.15866/IREBIC.V5I5.5984

A. Jimoh, A. Abdulkareem, A. Afolabi, J. O. Odigure, F. Emanuel

{"title":"Effects of Transesterification Parameters on the Biodiesel Produced from Crude Groundnut Oil","authors":"A. Jimoh, A. Abdulkareem, A. Afolabi, J. O. Odigure, F. Emanuel","doi":"10.15866/IREBIC.V5I5.5984","DOIUrl":"https://doi.org/10.15866/IREBIC.V5I5.5984","url":null,"abstract":"This study focused on optimization of biodiesel yield from crude groundnut oil through a two-stage transesterification process by investigating the effect of reaction parameters on the yield using a 24 experimental factorial design. The crude groundnut oil was pretreated to remove water and reduce its FFA by esterification using sulphuric acid and ethanol followed by transesterification using sodium hydroxide and ethanol. The optimum ethyl ester (biodiesel) yield of 99% was obtained at optimum conditions of 1:6 wt/wt oil to ethanol molar ratio, reaction temperature of 60oC, catalyst concentration of 1.0 wt% and reaction time of 90 minutes. The biodiesel produced was characterized for fuel properties that include kinetic viscosity, cetane number, flash point etc. and the determined values were found to be within the acceptable standard as recommended by the ASTM D 6751. Statistical analysis of the 24 factorial experimental results was conducted using ANOVA, it was evidently proved that the four (4) reaction parameters i.e. molar ratio, catalyst concentration, reaction temperature and time had significant effects on the yield of the ethyl ester with their percentage contribution and effects being 29.6667% and 4.35, 6.27121% and -2, 38.2213% and 4.9375, 3.12801% and 3.12801 respectively. Also, it was proved that interaction between the reactions parameters had significant effect on the ethyl ester yield except the interaction between molar ratio-catalyst concentration-reaction time whose p-value was below the 95% confident level. A first degree linear mathematical model was developed and it was seen to completely describe the system and the model was further used to develop software that predicts biodiesel yield from crude groundnut oil using two-step transesterification process with ethanol and KOH as the catalyst.","PeriodicalId":14377,"journal":{"name":"International Review of Biophysical Chemistry","volume":"12 1","pages":"114-124"},"PeriodicalIF":0.0,"publicationDate":"2014-10-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"86927038","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Technology and Engineering of Biodiesel Production: a Comparative Study between Microalgae and Other Non-Photosynthetic Oleaginous Microbes 生物柴油生产技术与工程:微藻与其他非光合产油微生物的比较研究

International Review of Biophysical Chemistry Pub Date : 2014-10-31 DOI: 10.15866/IREBIC.V5I5.5994

A. Yousuf, M. Hoque, M. Jahan, D. Pirozzi

引用次数: 7

Effect of Filling Kinetic of Sequencing Batch Reactor on the Poultry Wastewater Treatment 序批式反应器填充动力学对家禽废水处理的影响

International Review of Biophysical Chemistry Pub Date : 2014-10-31 DOI: 10.15866/IREBIC.V5I5.5996

Mauro de Paula Moreira, V. Perez, R. M. Sampaio, R. Alegre

引用次数: 0

Validation of Experimental Correlations for Estimating Solid Biofuel Properties from Proximate and Ultimate Analysis 从近似和最终分析估计固体生物燃料特性的实验相关性验证

International Review of Biophysical Chemistry Pub Date : 2014-08-31 DOI: 10.15866/IREBIC.V5I4.5980

Aitor Pablos, J. Saldarriaga, M. Amutio, R. Aguado, M. Olazar

引用次数: 0

Characterization of Lignocellulosic Biofuels by TGA 木质纤维素生物燃料的TGA表征

International Review of Biophysical Chemistry Pub Date : 2014-08-31 DOI: 10.15866/IREBIC.V5I4.5979

J. Saldarriaga, Aitor Pablos, R. Aguado, M. Amutio, M. Olazar

引用次数: 8

Surface Energetics of Protein Adsorption on to Chromatographic Supports 色谱载体上蛋白质吸附的表面能量学

International Review of Biophysical Chemistry Pub Date : 2014-06-30 DOI: 10.15866/IREBIC.V5I3.5974

M. Aasim, Poondi Rajesh Gavara, R. Vennapusa, M. F. Lahore

{"title":"Surface Energetics of Protein Adsorption on to Chromatographic Supports","authors":"M. Aasim, Poondi Rajesh Gavara, R. Vennapusa, M. F. Lahore","doi":"10.15866/IREBIC.V5I3.5974","DOIUrl":"https://doi.org/10.15866/IREBIC.V5I3.5974","url":null,"abstract":"Protein separation behavior during adsorption chromatography is governed by system thermodynamics and kinetic factors. Hydrophobic interaction chromatography (HIC) is widely utilized since many important biological products present a quite hydrophobic character. In this work, the interaction between a set of model proteins (n = 9) and a commercial adsorbent (Phenyl Sepharose FF, high substitution, GE Healthcare) was studied via extended DLVO (XDLVO) calculations. Psychochemical properties of both separand and adsorbent were gathered by contact angle determination and zeta potential measurements. Proteins were subjected to the mentioned measurements in the hydrated and the dehydrated state, so as to simulate protein properties in a low vs. high salt concentration milieu, respectively. In HIC, protein adsorption usually take place at high concentrations of ammonium sulphate (up to 1.7M) and protein desorption occurs by decreasing salt concentration in the mobile phase. The mentioned XDLVO approach allowed the calculation of the free energy of interaction vs. distance profiles between the interacting surfaces, in the aqueous environment provided by the operating mobile phase. XDLVO calculations were correlated with the actual chromatography behavior of the studied model proteins. This correlation revealed that these proteins can be segregated in two main groups, according to surface energy calculations and elution position during chromatography: i) strong binding showing a deeper secondary minimum energy >|0.20| kT ii) and weak binding having a small secondary minimum energy <|0.12| kT, thus calculations were able to predict early or late elution from a gradient chromatography experiment; the more the calculated interaction energy, the stronger will be protein binding and the later will be the elution time. The knowledge generated from these studies will generate a better understanding of real downstream bioprocess behavior which could, in turn, facilitate process design and optimization.","PeriodicalId":14377,"journal":{"name":"International Review of Biophysical Chemistry","volume":"1 1","pages":"61-69"},"PeriodicalIF":0.0,"publicationDate":"2014-06-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"86431959","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 5

The Use of Redox Mediators for Enhancing the Decolourisation of Orange G in a Sequencing Batch Reactor (SBR) 在序批式反应器(SBR)中使用氧化还原介质加强橙G脱色

International Review of Biophysical Chemistry Pub Date : 2014-06-30 DOI: 10.15866/IREBIC.V5I3.5975

M. Mehrdad, B. Bonakdarpour, B. Nasernejad

引用次数: 0

Advancement in the Structural Polymorphism of G-Quadruplexes g -四联体结构多态性研究进展

International Review of Biophysical Chemistry Pub Date : 2014-04-30 DOI: 10.15866/IREBIC.V5I2.4356

Mahima Kaushik, S. kaushik, S. Kukreti

{"title":"Advancement in the Structural Polymorphism of G-Quadruplexes","authors":"Mahima Kaushik, S. kaushik, S. Kukreti","doi":"10.15866/IREBIC.V5I2.4356","DOIUrl":"https://doi.org/10.15866/IREBIC.V5I2.4356","url":null,"abstract":"Genomes contain a large number of putative guanine-rich sequences, specifically on promoter regions, untranslated regions (UTR’s) and telomeres etc. that could form guanine-quadruplexes, and may serve as important structural and regulatory elements. They can also be the source of genomic instability which may lead to cancer, aging and human genetic diseases. Four guanines in the same plane, joined together with Hoogsteen hydrogen bonding, and stacked over one another resulting in guanine tetrads, give rise to an incredible class of G-quadruplexes. An extensive range of G-quadruplex structures is well documented, where they differ in number of strands (uni, bi, or tetramolecular), conformations (parallel, antiparallel or mixed), shapes (chair or basket form), or types of loops (edgewise/ lateral, diagonal, double chain reversal/ propeller, or V-shaped loops) etc. With advancements in the techniques, various new multistranded G-rich DNA structures are enriching the DNA structural databases. The most recent ones are (3+1) G-quadruplex, Tri-G-quadruplex, G-triplex DNA etc. which actually add to the diversity of G-quadruplex structures. Exploring their polymorphism with various biophysical and biochemical techniques has hence become extremely important. This review mainly focuses on the discussion of these unusual and comparatively new polymorphic G-quadruplex DNA structures. The robustness of these unique (3+1) G-quadruplex or G-triplex or tri-G-quadruplex structures actually can be exploited for providing a strong foundation for the designing of structure-specific drugs. Recently, G-quadruplex structures have been quantitatively visualized in human cells by engineering structure-specific antibody. Considering these developments, the mapping of G-quadruplex structures in genome may now be possible, with a goal of controlling the gene functions or other cellular processes, which might be involved in diseases like cancer.","PeriodicalId":14377,"journal":{"name":"International Review of Biophysical Chemistry","volume":"13 1","pages":"37-46"},"PeriodicalIF":0.0,"publicationDate":"2014-04-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"91255677","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1