Korean Journal of Clinical Microbiology最新文献_第2页

Basic Concepts of Bacterial Taxonomy 细菌分类学的基本概念

Korean Journal of Clinical Microbiology Pub Date : 2012-09-01 DOI: 10.5145/KJCM.2012.15.3.79

Young Sook Kim, S. Jang

引用次数: 3

Laboratory Experience in Phenotypic and Molecular Identification of Blastomyces dermatitidis First Isolated in Korea 韩国首次分离的皮炎芽孢菌的表型和分子鉴定的实验室经验

Korean Journal of Clinical Microbiology Pub Date : 2012-09-01 DOI: 10.5145/KJCM.2012.15.3.114

Kyung Sun Park, C. Ki, N. Lee

引用次数: 4

Nontuberculous Mycobacteria Isolated from Respiratory Specimens during Recent Two Years: Distribution and Clinical Significance 近两年呼吸道标本分离非结核分枝杆菌的分布及临床意义

Korean Journal of Clinical Microbiology Pub Date : 2012-09-01 DOI: 10.5145/KJCM.2012.15.3.98

Minkyung Lee, Y. Seo, J. Jeong, P. Park, Kyung-Hee Kim, J. Ahn, Jin Yong Kim, Jeong Woong Park

{"title":"Nontuberculous Mycobacteria Isolated from Respiratory Specimens during Recent Two Years: Distribution and Clinical Significance","authors":"Minkyung Lee, Y. Seo, J. Jeong, P. Park, Kyung-Hee Kim, J. Ahn, Jin Yong Kim, Jeong Woong Park","doi":"10.5145/KJCM.2012.15.3.98","DOIUrl":"https://doi.org/10.5145/KJCM.2012.15.3.98","url":null,"abstract":"Background: The isolation of nontuberculous mycobacteria (NTM) has been increasing worldwide as well as its clinical importance. The aim of this study was to investigate the distribution and clinical significance of NTM that has been isolated from respiratory specimens during a recent two-year period at a tertiary hospital. Methods: We analyzed respiratory samples that were obtained between January 2009 and December 2010 for AFB culture. We retrospectively reviewed the electronic medical records of these patients to obtain both clinical and radiologic information. NTM pulmonary disease was defined by using the guidelines provided by the America Thoracic Society/Infectious Diseases Society of America. Results: Among the 1,601 specimens that resulted in a positive AFB culture, 310 (19.4%) were NTM. In 189 patients, the most common isolate was M. aviumintracellulare complex (MAC) (127, 67.2%), which was then followed by M. abscessus (31, 16.4%), M. fortuitum (10, 5.3%), M. kansasii (9, 4.8%), and other NTM species. Of these, 93 (49.2%) patients were diagnosed with NTM pulmonary disease. MAC, M. abscessus, and M. kansasii were more virulent than the other species. None of the cases of NTM pulmonary disease were caused by M. fortuitum, M. chelonae, M. peregrinum, M. terrae complex, or M. gordonae. Conclusion: In Korea, the prevalence of NTM isolates is increasing, as are the cases of pulmonary disease. The pathogenic potential of NTM differs enormously by species and as a result the treatment of NTM lung disease depends on which species has caused the infection. The isolation and identification of NTM isolated from respiratory specimens are mandatory in order for clinical microbiology laboratories to make an accurate diagnosis and suggest the proper treatment of the NTM disease. (Korean J Clin Microbiol 2012;15:98-103)","PeriodicalId":143093,"journal":{"name":"Korean Journal of Clinical Microbiology","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2012-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"125340773","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 5

Epidemiologic Characteristics of Extrapulmonary Tuberculosis in Korea, 1995-2010: Microbiological Diagnosis versus Clinical Diagnosis 1995-2010年韩国肺外结核流行病学特征:微生物学诊断与临床诊断

Korean Journal of Clinical Microbiology Pub Date : 2012-09-01 DOI: 10.5145/KJCM.2012.15.3.92

Kyoung-Jin Park, Kyung Sun Park, N. Lee

{"title":"Epidemiologic Characteristics of Extrapulmonary Tuberculosis in Korea, 1995-2010: Microbiological Diagnosis versus Clinical Diagnosis","authors":"Kyoung-Jin Park, Kyung Sun Park, N. Lee","doi":"10.5145/KJCM.2012.15.3.92","DOIUrl":"https://doi.org/10.5145/KJCM.2012.15.3.92","url":null,"abstract":"Background: The aim of the present study was to investigate the epidemiologic characteristics of extrapulmonary tuberculosis (EPTB) in Korea. In addition, the results of culture-confirmed (CC) EPTB were compared with those of clinically-diagnosed (CD) EPTB. Methods: We retrospectively reviewed non-duplicate data of tuberculosis from the Samsung Medical Center from 1995 to 2010. A total of 6,249 and 38,726 cases of tuberculosis were CC and CD EPTB cases, respectively. The cases were categorized according to the type of specimen or by the clinicallyaffected sites. Results: The proportions of EPTB among all tuberculosis cases were 12% (745/6,249) and 22% (8,608/ 38,726) of the CC and CD cases, respectively. The distribution of both age and gender between pulmonary tuberculosis (PTB) and EPTB cases were significantly different (P＜0.001). The most common types of EPTB were tuberculous lymphadenitis, pleural TB, and abdominal TB. Pleural involvement was more common in males, while lymph node involvement was observed more frequently in females in both the CC and CD cases (M/F ratio in regards to pleura were 1.63 and 2.08, while M/F ratio in regards to the lymph node were 0.46 and 0.54). Conclusion: The dataset of EPTB cases in Korea was first evaluated over a 16-year period and compared the cases of CC EPTB to those of CD EPTB. The epidemiologic characteristics of EPTB were different from that of PTB as well as the EPTB of other countries. The present study might provide useful information regarding the epidemiology of EPTB in Korea and other countries. (Korean J Clin Microbiol 2012;15:92-97)","PeriodicalId":143093,"journal":{"name":"Korean Journal of Clinical Microbiology","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2012-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"130731342","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

Number of Blood Cultures per 1,000 Patient Days at University-Affiliated Hospitals in Korea 韩国大学附属医院每1000名患者日的血液培养次数

Korean Journal of Clinical Microbiology Pub Date : 2012-06-01 DOI: 10.5145/KJCM.2012.15.2.67

E. Kim, J. Shin, Sunjoo Kim, N. Lee, J. Cho, S. Koo, N. Ryoo, S. Joo

引用次数: 3

Epidemiologic Features of Parainfluenza Virus Type 1, 2 and 3 Infection in Seoul and a Neighboring Area, 2008-2011 2008-2011年首尔及邻近地区副流感病毒1、2、3型感染的流行病学特征

Korean Journal of Clinical Microbiology Pub Date : 2012-06-01 DOI: 10.5145/KJCM.2012.15.2.54

Hye-Jin Lee, C. Lee, M. Nam, K. Roh, Soo-Young Yoon, C. Lim, Yunjung Cho, Young Kee Kim, Kap-No Lee, Young M. Yoo

引用次数: 3

Evaluation of Matrix-Assisted Laser Desorption Ionization-Time of Flight Mass Spectrometry for Identification of Aerobic Bacteria in a Clinical Microbiology Laboratory 基质辅助激光解吸电离飞行时间质谱法鉴定临床微生物实验室需氧菌的评价

Korean Journal of Clinical Microbiology Pub Date : 2012-06-01 DOI: 10.5145/KJCM.2012.15.2.60

Myungsook Kim, M. Kwon, Hae-Sun Chung, Yangsoon Lee, D. Yong, S. Jeong, Kyungwon Lee, Y. Chong

{"title":"Evaluation of Matrix-Assisted Laser Desorption Ionization-Time of Flight Mass Spectrometry for Identification of Aerobic Bacteria in a Clinical Microbiology Laboratory","authors":"Myungsook Kim, M. Kwon, Hae-Sun Chung, Yangsoon Lee, D. Yong, S. Jeong, Kyungwon Lee, Y. Chong","doi":"10.5145/KJCM.2012.15.2.60","DOIUrl":"https://doi.org/10.5145/KJCM.2012.15.2.60","url":null,"abstract":"Background: Matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) has been used for the identification of bacteria worldwide. To our knowledge, the evaluation of MALDI-TOF MS for the identification of bacteria in Korea has not been studied. In this paper we compared the identification results of aerobic bacteria using MALDI-TOF MS to those results using conventional biochemical methods. Methods: We evaluated the performance of a MALDITOF MS system (Bruker Daltonics, Leipzig, Germany) on consecutive aerobic isolates collected from January to February of 2011 which were identified using conventional methods (biochemical testing and commercial identification kits). Either directly smearing onto the target plate or protein extraction methods were additionally used if no reliable or discordant results were obtained. Results: Among 523 isolates tested, 506 (97%) isolates had valid scores (≥2.0), 11 (2%) isolates gave intermediate scores (1.7≤ score <2.0), and 6 (1%) isolates yielded no reliable identification (score <1.7). Of the 506 valid results (score ≥2.0) by MALDI-TOF MS, the identification matched at the species level in 486 (96%) isloates, matched at the genus level in 17 (3%) isloates, and was discordant at the genus and species levels in 3 (1%) isloates. Conclusion: The overall matching rate at the species level of MALDI-TOF MS was very high. When MALDI-TOF MS did not yield reliable results by direct smear, additional direct smears or protein extraction methods could be used to obtain better results. Our results showed that MALDI-TOF MS is a very useful method for the identification of aerobic bacteria isolated in clinical microbiology laboratories. (Korean J Clin Microbiol 2012;15:60-66)","PeriodicalId":143093,"journal":{"name":"Korean Journal of Clinical Microbiology","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2012-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"131061622","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 7

Sentinel Surveillance and Molecular Epidemiology of Multidrug Resistance Bacteria 多药耐药细菌的哨点监测和分子流行病学

Korean Journal of Clinical Microbiology Pub Date : 2012-06-01 DOI: 10.5145/KJCM.2012.15.2.43

Y. Lee, H. Kim, J. Yoo, J. Yoo, Younghee Jung

{"title":"Sentinel Surveillance and Molecular Epidemiology of Multidrug Resistance Bacteria","authors":"Y. Lee, H. Kim, J. Yoo, J. Yoo, Younghee Jung","doi":"10.5145/KJCM.2012.15.2.43","DOIUrl":"https://doi.org/10.5145/KJCM.2012.15.2.43","url":null,"abstract":"The global emergence and spread of multidrug resistant bacterial infections in communities and hospitals has become an important issue in public health. The resistance rate of gram-positive cocci to vancomycin and the resistance rate of several gram-negative bacilli against cefotaxime and carbapenem have been continuously increasing. Surveillance of antimicrobial resistance is essential for providing information on the magnitude of and trend in multidrug resistance. Therefore, beginning 2011, more robust and effective management is to be legally required for six multidrug-resistant bacteria that have been linked to healthcare-related infections: vancomycin-resistant Staphylococcus aureus (VRSA), vancomycinresistant enterococci (VRE), methicillinresistant S. aureus (MRSA), multidrug-resistant Pseudomonas aeruginosa (MRPA), multidrug-resistant Acinetobacter baumannii (MRAB), and carbapenem-resistant Enterobactericeae (CRE). We have also performed laboratory-based sentinel surveillance for VRSA/VISA since 2002 and carbapenemase-producing Enterobacteriaceae since November, 2010. This article reviews the national surveillance programs, and molecular epidemiology of multidrug-resistant bacteria. (Korean J Clin Microbiol 2012;15:43-48)","PeriodicalId":143093,"journal":{"name":"Korean Journal of Clinical Microbiology","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2012-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"116335619","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 3

Primary Cutaneous Cryptococcosis in a Patient with Iatrogenic Cushing's Syndrome: A Case Report and Review of the Literature 医源性库欣综合征患者原发性皮肤隐球菌病1例报告及文献复习

Korean Journal of Clinical Microbiology Pub Date : 2012-06-01 DOI: 10.5145/KJCM.2012.15.2.70

Y. Ko, M. Hong, C. Park, H. Moon, M. Hur, Y. Yun

{"title":"Primary Cutaneous Cryptococcosis in a Patient with Iatrogenic Cushing's Syndrome: A Case Report and Review of the Literature","authors":"Y. Ko, M. Hong, C. Park, H. Moon, M. Hur, Y. Yun","doi":"10.5145/KJCM.2012.15.2.70","DOIUrl":"https://doi.org/10.5145/KJCM.2012.15.2.70","url":null,"abstract":"Cryptococcus neoformans (C. neoformans) is a ubiquitous yeast-like fungus that has been a common opportunistic human pathogen, especially in immunocompromised patients. Although skin lesions due to C. neoformans are found in 10-15% of patients with systemic cryptococcosis, primary cutaneous cryptococcosis without systemic infection is rare and now considered a distinct clinical entity. We report a case of primary cutaneous cryptococcosis in a patient with iatrogenic Cushing’s syndrome. A 73-year-old woman presented with pain and discharge from lesions on her left forearm. The patient had been treated with oral corticosteroids for 20 years, and as a result had developed iatrogenic Cushing’s syndrome. A skin fragment of the ulcer was cultured, and the encapsulated fungus were isolated and identified as C. neoformans using a Vitek2 system (Vitek2 ID-YST, bioMerieux, France) and API 20C (bioMerieux). Concurrent blood and urine cultures were negative for growth. At first, she was treated with antibiotics due to suspicion of cellulitis. After surgical resection and treatment with systemic and oral fluconazole, her wound was improved with scar. Primary cutaneous cryptococcosis should be considered when skin lesions are not responsive to antibiotics and accurate identification is important for proper treatment. (Korean J Clin Microbiol 2012;15:70-73)","PeriodicalId":143093,"journal":{"name":"Korean Journal of Clinical Microbiology","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2012-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"125218268","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 3

A Case of Anaerobiospirillum succiniciproducens Isolated from Blood Culture 产琥珀酸厌氧菌血培养分离一例

Korean Journal of Clinical Microbiology Pub Date : 2012-06-01 DOI: 10.5145/KJCM.2012.15.2.74

Woo Ri Jang, C. Nahm, Y. Moon, Young Je, D. Yong, Jin Ju Kim

引用次数: 1