Korean Journal of Clinical Microbiology最新文献

An Unusual Feature of Malaria: Exflagellated Microgametes of Malarial Parasites in Human Peripheral Blood 疟疾的一个不寻常的特征:人外周血中疟原虫的脱落小配子

Korean Journal of Clinical Microbiology Pub Date : 2012-12-01 DOI: 10.5145/KJCM.2012.15.4.151

Kyungmin Lee, J. Ham, Bongbo Seo, Yu Kyung Kim, Won-Kil Lee

{"title":"An Unusual Feature of Malaria: Exflagellated Microgametes of Malarial Parasites in Human Peripheral Blood","authors":"Kyungmin Lee, J. Ham, Bongbo Seo, Yu Kyung Kim, Won-Kil Lee","doi":"10.5145/KJCM.2012.15.4.151","DOIUrl":"https://doi.org/10.5145/KJCM.2012.15.4.151","url":null,"abstract":"Exflagellation of the malaria parasite microgametocyte usually occurs in the gut cavity of Anopheles mosquitoes following an infective blood meal. Exflagellation is a very rare event in human blood. Due to its rarity, the appearance of this structure in a peripheral blood smear will easily create a diagnostic dilemma. We report a case of malaria with exflagellated microgametes in human blood that was initially mistaken for a double infection of Plasmodium and another blood flagellate. The patient was a 29-year-old Parkistani man presenting with fluctuating fever accompanied by chills and fatigue for 4 days. Initial peripheral blood smear examination showed a number of Plasmodium ring forms, trophozoites, and gametocytes. Additionally, several filamentous structures resembling blood flagellates were seen. With these features, an initial diagnostic impression of combined infection of malaria and blood flagellate was made. Later, we determined that these structures resembling blood flagellates were exflagellated microgametes of malarial parasite. Therefore, the knowledge that exflagellation may appear in human blood with Plasmodium species infection and being more familiar with differentiation of the morphologic features of other species infection can prevent further possible misinterpretation. (Korean J Clin Microbiol 2012;15:151-153)","PeriodicalId":143093,"journal":{"name":"Korean Journal of Clinical Microbiology","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2012-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"121889433","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Lung Abscess and Bacteremia Caused by Neisseria flavescens and Streptococcus sanguis in Patient with Idiopathic Hypereosinophilic Syndrome 特发性高嗜酸性粒细胞综合征患者由黄奈瑟菌和血链球菌引起的肺脓肿和菌血症

Korean Journal of Clinical Microbiology Pub Date : 2012-12-01 DOI: 10.5145/KJCM.2012.15.4.147

J. Kong, S. Shin, Su Eun Park, H. Park, Jongyoun Yi, Shine-Young Kim, S. Son

引用次数: 3

A Case of Diabetic Foot Ulcer Caused by Arcanobacterium haemolyticum and Streptococcus agalactiae 溶血隐菌和无乳链球菌所致糖尿病足溃疡1例

Korean Journal of Clinical Microbiology Pub Date : 2012-12-01 DOI: 10.5145/KJCM.2012.15.4.143

M. Choi, D. Kim, Sam-Im Choi, Y. Cho, Hye Soo Lee

引用次数: 0

Diversity of Integrons Carrying blaVIM-2 Cassette in Pseudomonas spp. and Acinetobacter spp. 假单胞菌和不动杆菌携带blaVIM-2盒式整合子的多样性

Korean Journal of Clinical Microbiology Pub Date : 2012-12-01 DOI: 10.5145/KJCM.2012.15.4.131

J. Yum, H. Shin, D. Yong, Y. Chong

{"title":"Diversity of Integrons Carrying blaVIM-2 Cassette in Pseudomonas spp. and Acinetobacter spp.","authors":"J. Yum, H. Shin, D. Yong, Y. Chong","doi":"10.5145/KJCM.2012.15.4.131","DOIUrl":"https://doi.org/10.5145/KJCM.2012.15.4.131","url":null,"abstract":"Background: Metallo-β-lactamase-mediated carbapenem resistance has been increasingly reported in Pseudomonas, Acinetobacter and other Gram-negative bacilli (GNB) in many countries. A few studies showed highly variable structure of MBL-gene cassette-carrying integrons. The aim of this study was to determine the structure of blaVIM-2-carrying integrons in Pseudomonas and Acinetobacter. Methods: blaVIM-2-carrying GNB were isolated at a Korean hospitals during the years 1995-1999 and 2005. The size of blaVIM-2-carrying integrons was estimated by the PCR products. Representative integrons were sequenced by the dideoxy-chain termination method. The MICs of antimicrobial agents were tested by the CLSI agar dilution methods. Results: During the years 1995-1999 and 2005, the approximate size of the blaVIM-2-carrying class 1 integrons was 3-7 kb in 35 Pseudomonas isolates and 3-5 kb in 24 Acinetobacter isolates. The integrons carried one-five resistance gene cassettes in addition to the blaVIM-2 cassette. Other resistance gene cassettes found were blaOXA-1, aacA1, aac(6’)-I, and aac(6’)-II. Interestingly, sequences homologous to part of a putative class II intron were inserted into the recombination site of the last cassette in four of nine integrons. The class 1 integron from P. aeruginosa isolates had fused orf/IntI1 in a downstream leftward inverted repeat (IRi). Conclusion: According to period, the size and structure of blaVIM-2-carrying integrons are quite variable, but an identical one is also present in a different genus, indicating high mobility of the blaVIM-2 cassette and horizontal transfer of the whole integron. We suggest that the class 1 integron containing the blaVIM-2 gene is spreading horizontally among Gramnegative bacilli and is undergoing continuous development in Korea. (Korean J Clin Microbiol 2012;15:131-138)","PeriodicalId":143093,"journal":{"name":"Korean Journal of Clinical Microbiology","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2012-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"114854742","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 3

Differential Time to Positivity and Semi-Quantitative Culture of Catheter Segments for Diagnosing Catheter-Related Bloodstream Infections 导管节段阳性和半定量培养的差异时间诊断导管相关血流感染

Korean Journal of Clinical Microbiology Pub Date : 2012-12-01 DOI: 10.5145/KJCM.2012.15.4.125

S. Oh, Miae Lee

{"title":"Differential Time to Positivity and Semi-Quantitative Culture of Catheter Segments for Diagnosing Catheter-Related Bloodstream Infections","authors":"S. Oh, Miae Lee","doi":"10.5145/KJCM.2012.15.4.125","DOIUrl":"https://doi.org/10.5145/KJCM.2012.15.4.125","url":null,"abstract":"Background: Catheter-related bloodstream infection (CRBSI) is one of the leading types of infection, with a significant morbidity and mortality rate. We evaluated the differential time to positivity (DTP) and semi-quantitative culture of catheter segments (SQCC) as a method for diagnosing CRBSI. Methods: From January 2010 to August 2011, 155 positive paired blood cultures which had the same organism isolated from blood cultures drawn simultaneously through the central venous catheter (CVC) and the peripheral vein were included. Positive DTP represents a DTP of least 120 min earlier for the time to detection of CVC draw than that of a peripheral vein draw. We evaluated the clinical utility of DTP and SQCC for diagnosing CRBSIs, which were further divided into two groups: confirmed (either by DTP or SQCC) and non-confirmed CRBSIs (neither DTP nor SQCC positive). Results: Sixty-five percent (100/155) of episodes were confirmed to CRBSIs. In CRBSIs, Gram-positive cocci accounted for 61% of cases, non-fermenting Gram-negative bacilli represented 10%, Enterobacteriaceae for 10%, yeasts for 15%, and others for 4%. Among the confirmed CRBSI cases, 22 were both positive with DTP and SQCC, 30 cases were positive with DTP only, 12 cases were positive with SQCC only, and 36 cases which did not undergo SQCC analysis were DTP positive. The sensitivities of the DTP and SQCC techniques were 88.0% (88/100) and 53.1% (34/64), respectively. Conclusion: The differential time to positivity was more sensitive than the semi-quantitative culture of catheter segments for the diagnosis of CRBSIs. DTP is useful for diagnosing CRBSIs without removal of the catheter. (Korean J Clin Microbiol 2012;15:125130)","PeriodicalId":143093,"journal":{"name":"Korean Journal of Clinical Microbiology","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2012-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"134130559","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

Evaluation of the AdvanSure MDR-TB GenoBlot Assay for Detection of Rifampin and Isoniazid Resistant Mycobacterium tuberculosis Complex in Respiratory Specimens advanure耐多药结核基因斑点法检测呼吸道标本中利福平和异烟肼耐药结核分枝杆菌复合体的评价

Korean Journal of Clinical Microbiology Pub Date : 2012-12-01 DOI: 10.5145/KJCM.2012.15.4.117

Jayoung Kim, Yeon-Joon Park, N. Lee, Chulhun L. Chang, Miae Lee, Jong‐Hee Shin

{"title":"Evaluation of the AdvanSure MDR-TB GenoBlot Assay for Detection of Rifampin and Isoniazid Resistant Mycobacterium tuberculosis Complex in Respiratory Specimens","authors":"Jayoung Kim, Yeon-Joon Park, N. Lee, Chulhun L. Chang, Miae Lee, Jong‐Hee Shin","doi":"10.5145/KJCM.2012.15.4.117","DOIUrl":"https://doi.org/10.5145/KJCM.2012.15.4.117","url":null,"abstract":"Background: We evaluated the performance of the AdvanSure MDR-TB GenoBlot Assay kit (AdvanSure MDR-TB, LG Life Science, Korea) to detect mutations related to rifampin (RFP)and isoniazid (INH)-resistant Mycobacterium tuberculosis complex in respiratory specimens. Methods: From February 2010 to June 2010, a total of 542 M. tuberculosis clinical isolates were collected from pulmonary tuberculosis patients in six university hospitals across Korea. We analyzed the conventional drug susceptibility testing (DST) and compared the results with those of the AdvanSure MDR-TB. Results: Compared with the conventional DST, the overall agreement rates, sensitivity, and specificity were 98.2% (532/542), 84.6% (33/39), and 99.2% (499/503), respectively, for RFP resistance and 96.1% (521/542), 79.7% (59/74), 98.7% (462/468), respectively, for INH resistance. The three common rpoB mutations were rpoB S531L (53.8%), rpoB D516V (15.4%) and rpoB H526R (7.7%) in RFP-resistant strains. For INH resistance, the katG S315T mutation (58.1%) was the most common, followed by inhA C-15T (23.0%) and katG S315N (4.1%). Conclusion: The AdvanSure MDR-TB showed high concordance with the conventional DST and would be helpful for early detection of RFP and INH resistance, although it requires improved sensitivity. (Korean J Clin Microbiol 2012;15:117-124)","PeriodicalId":143093,"journal":{"name":"Korean Journal of Clinical Microbiology","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2012-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"130313052","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 3

Haemophilus parainfluenzae Infective Endocarditis Confirmed by 16S rRNA Sequence Analysis from Culture Negative Tissue 培养阴性组织16S rRNA序列分析证实副流感嗜血杆菌感染性心内膜炎

Korean Journal of Clinical Microbiology Pub Date : 2012-12-01 DOI: 10.5145/KJCM.2012.15.4.139

Kyoung-Jin Park, Kyung Sun Park, Soo-Han Choi, Yae-Jean Kim, C. Ki, I. Kang, N. Lee

引用次数: 2

A Case of Bacteremia Due to Microbacterium oleivorans Identified by 16S rRNA Sequencing Analysis 用16S rRNA测序分析鉴定1例橄榄微杆菌菌血症

Korean Journal of Clinical Microbiology Pub Date : 2012-09-01 DOI: 10.5145/KJCM.2012.15.3.110

Bohyun Kim, Mi-Kyung Lee

引用次数: 3

Evaluation of a ChromID C. difficile Agar for the Isolation of Clostridium difficile 难辨梭菌培养基对难辨梭菌分离的评价

Korean Journal of Clinical Microbiology Pub Date : 2012-09-01 DOI: 10.5145/KJCM.2012.15.3.88

J. Yim, S. Hwang, Myungsook Kim, H. Lim, Saeam Shin, Hae-Sun Chung, Heejung Kim, Kyungwon Lee

{"title":"Evaluation of a ChromID C. difficile Agar for the Isolation of Clostridium difficile","authors":"J. Yim, S. Hwang, Myungsook Kim, H. Lim, Saeam Shin, Hae-Sun Chung, Heejung Kim, Kyungwon Lee","doi":"10.5145/KJCM.2012.15.3.88","DOIUrl":"https://doi.org/10.5145/KJCM.2012.15.3.88","url":null,"abstract":"Background: Clostridium difficile is the main etiologic agent of antibiotic-associated diarrhea and the most common cause of hospital-acquired diarrhea. Recently, the incidence of C. difficile infections (CDI) has increased and new highly virulent C. difficile strains have emerged. Therefore, accurate and rapid diagnosis is needed. We compared the results of using chromID C. difficile (chromID CD, bioMerieux, France) with the conventional C. difficile Selective Agar (CDSA; BD, USA) for the isolation of C. difficile. Methods: A total of 738 stool specimens of suspected CDI patients at the Severance Hospital from July to August 2011 were inoculated onto CDSA. Among them, 104 stool specimens revealed colonies on CDSA that were then re-inoculated onto chromID CD. The stool samples were stored at −20 o C until the time of the re-inoculation. Cultured agars were interpreted after 24 hrs and 48 hrs, respectively. Species identification was performed on the basis of colony characteristics on agar plates as well as the ATB 32A system (API System SA, France). Results: The recovery rates of CDSA and chromID CD were 30.1% and 77.5% after 24 hrs, and 77.5% and 98.6% after 48 hrs, respectively. All of the C. difficile isolates were recovered as typical gray/black colonies on chromID CD. Conclusion: The performance of chromID CD for the isolation of C. difficile was better than that of conventional CDSA. The chromID CD could provide easy and sensitive detection of C. difficile even after 24hrs of incubation. (Korean J Clin Microbiol 2012; 15:88-91)","PeriodicalId":143093,"journal":{"name":"Korean Journal of Clinical Microbiology","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2012-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"134579156","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 4

Detection of Trichomonas vaginalis, Gardnerella vaginalis, and Candida Species in Affirm VPIII, Papanicolaou Smear Test and Gram Stain 阴道毛滴虫、阴道加德纳菌和念珠菌在确认型VPIII、巴氏涂片试验和革兰氏染色中的检测

Korean Journal of Clinical Microbiology Pub Date : 2012-09-01 DOI: 10.5145/KJCM.2012.15.3.104

K. Kim, Tae-Hyoung Kim, Jun Hyung Lee, Tae Jin Lee, Mi-Kyung Lee

{"title":"Detection of Trichomonas vaginalis, Gardnerella vaginalis, and Candida Species in Affirm VPIII, Papanicolaou Smear Test and Gram Stain","authors":"K. Kim, Tae-Hyoung Kim, Jun Hyung Lee, Tae Jin Lee, Mi-Kyung Lee","doi":"10.5145/KJCM.2012.15.3.104","DOIUrl":"https://doi.org/10.5145/KJCM.2012.15.3.104","url":null,"abstract":"Background: Infectious vaginitis is caused primarily by three different groups of microbial pathogens (Trichomonas vaginalis, Candida spp., and Gardnerella vaginalis). The objective of this study was to compare the Affirm VPIII assay using a DNA hybridization technique with the Papanicolaou (Pap) smear test and the Gram stain in the detection and identification of these three organisms. Methods: A total of 300 vaginal samples were collected from women that were either symptomatic for vaginitis or asymptomatic women that were being seen for routine obstetric or gynecological care. The presence of T. vaginalis, Candida spp., and G. vaginalis was evaluated by using the Affirm VIII assay (Becton Dickinson, USA), Pap smear test, and Gram stain method, respectively. Results: With the Affirm VPIII assay, 1 (0.3%) patient tested positive for T. vaginalis, 99 (33.0%) patients were positive for G vaginalis, and 18 (6.0%) were positive for Candida spp. The detection rates of Trichomonas infection, bacterial vaginosis and candidiasis by the Pap smear test and Gram stain method were 0.7% versus 0%, 16.3% versus 35.7%, and 1.7% versus 9.7%, respectively. The differences between the detection rates of the above three organisms between the Pap smear test and the Gram stain method were statistically significant (P<0.05). Conclusion: The Affirm VPIII assay was more sensitive than the Pap smear test and more specific than the Gram stain method for the detection and identification of these three organisms. In addition, the results of the Affirm VPIII assay are quick to obtain and are simple and easy to interpret. (Korean J Clin Microbiol 2012;15:104-109)","PeriodicalId":143093,"journal":{"name":"Korean Journal of Clinical Microbiology","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2012-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"133549247","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1