International Journal of Radiation Biology最新文献

{"title":"Analysis of the urinary metabolic profiles in irradiated rats treated with Activated Protein C (APC), a potential mitigator of radiation toxicity.","authors":"Shivani Bansal, Sunil Bansal, Brian L Fish, Yaoxiang Li, Xiao Xu, Jose A Fernandez, John H Griffin, Heather A Himburg, Marjan Boerma, Meetha Medhora, Amrita K Cheema","doi":"10.1080/09553002.2023.2182001","DOIUrl":"10.1080/09553002.2023.2182001","url":null,"abstract":"<p><strong>Purpose: </strong>The goal of the current study was to identify longitudinal changes in urinary metabolites following IR exposure and to determine potential alleviation of radiation toxicities by administration of recombinant APC formulations.</p><p><strong>Materials and methods: </strong>Female adult WAG/RijCmcr rats were irradiated with 13.0 Gy leg-out partial body X-rays; longitudinally collected urine samples were subject to LC-MS based metabolomic profiling. Sub-cohorts of rats were treated with three variants of recombinant APC namely, rat wildtype (WT) APC, rat 3K3A mutant form of APC, and human WT APC as two bolus injections at 24 and 48 hours post IR.</p><p><strong>Results: </strong>Radiation induced robust changes in the urinary profiles leading to oxidative stress, severe dyslipidemia, and altered biosynthesis of PUFAs, glycerophospholipids, sphingolipids, and steroids. Alterations were observed in multiple metabolic pathways related to energy metabolism, nucleotide biosynthesis and metabolism that were indicative of disrupted mitochondrial function and DNA damage. On the other hand, sub-cohorts of rats that were treated with rat wildtype-APC showed alleviation of radiation toxicities, in part, at the 90-day time point, while rat 3K3A-APC showed partial alleviation of radiation induced metabolic alterations 14 days after irradiation.</p><p><strong>Conclusions: </strong>Taken together, these results show that augmenting the Protein C pathway and activity via administration of recombinant APC may be an effective approach for mitigation of radiation induced normal tissue toxicity.</p>","PeriodicalId":14261,"journal":{"name":"International Journal of Radiation Biology","volume":"99 7","pages":"1109-1118"},"PeriodicalIF":2.6,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10330346/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10127900","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Manganese facilitated cGAS-STING-IFNI pathway activation induced by ionizing radiation in glioma cells.","authors":"Yuping He, Ying Yang, Wenpeng Huang, Shuangyu Yang, Xuefei Xue, Kun Zhu, Huiling Tan, Ting Sun, Wei Yang","doi":"10.1080/09553002.2023.2232011","DOIUrl":"10.1080/09553002.2023.2232011","url":null,"abstract":"<p><strong>Purpose: </strong>After irradiation, double-stranded DNA leaked into the cytoplasm activates the cyclic GMP-AMP synthase (cGAS)-stimulator of interferon genes (STING) pathway, leading to the production of type I interferon (IFNI). In this study, we sought to probe the effect of ionizing radiation on activity of cGAS-STING-IFNI pathway in normoxic or hypoxic glioma cells and explore a more effective method to activate the signaling pathway, thereby activating the anti-tumor immune response and improving the therapeutic effect of radiotherapy for glioma.</p><p><strong>Materials and methods: </strong>Human glioma cells U251 and T98G cultured in normoxia or hypoxia (1% O₂) were irradiated with different doses of X-ray. The relative expressions of cGAS, IFN-I stimulated genes (ISGs), and three-prime repair exonuclease 1 (TREX1) were detected by qPCR. The expression levels of interferon regulatory factor 3 (IRF3) and p-IRF3 proteins were detected by Western blot. The production of cGAMP and IFN-β in the supernatant was detected by ELISA assay. U251 and T98G cell lines with stable knockdown of TREX1 were established after transfection with lentivirus vectors. EdU cell proliferation assay was used to screen suitable metal ions concentrations. The phagocytosis of DCs was observed by immunofluorescence microscope. The phenotype of DCs was detected by flow cytometry. The migration ability of DCs was detected by a transwell experiment.</p><p><strong>Results: </strong>We found that cytosolic dsDNA, 2'3'-cGAMP, cGAS and ISGs expression, and IFN-β in cell supernatant were all increased with the doses of X-ray in the range of 0-16 Gy in normoxic glioma cells. Nevertheless, hypoxia significantly inhibited the radiation-induced dose-dependent activation of cGAS-STING-IFNI pathway. Furthermore, manganese (II) ion (Mn²⁺) significantly improved cGAS-STING-IFNI pathway activation induced by X-ray in both normoxic and hypoxic glioma cells, thereby promoting the maturation and migration of DCs.</p><p><strong>Conclusions: </strong>The responses of cGAS-STING-IFNI pathway to ionizing radiation were mainly investigated under normoxic condition, but the experiments described here indicated that hypoxia could hinder the pathway activation. However, Mn²⁺ showed radiosensitizing effects on the pathway under either normoxic or hypoxic conditions demonstrating its potential as a radiosensitizer for glioma through activating an anti-tumor immune response.</p>","PeriodicalId":14261,"journal":{"name":"International Journal of Radiation Biology","volume":" ","pages":"1890-1907"},"PeriodicalIF":2.6,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10147061","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Correction.","authors":"","doi":"10.1080/09553002.2023.2181604","DOIUrl":"https://doi.org/10.1080/09553002.2023.2181604","url":null,"abstract":"It has recently come to the authors’ attention that the final activity concentrations (kBq/ml) reported for the treatment of the cells in the proliferation and colony formation assays had not been properly updated. For those assays, the cells were first treated with unconjugated antibody (blocking) or just medium, having the final volumes/well be greater by 60 ml and 1ml for each assay, respectively. This corresponds to actual activity concentrations of (0, 0.116, 0.231, 0.578, 1.16, 1.74, 2.31 and 4.63) kBq/ml and (0, 0.123, 0.247, 0.617, 1.23 and 2.47) kBq/ml, instead of (0, 0.185, 0.370, 0.925, 1.85, 2.78, 3.70 and 7.40) kBq/ml and (0, 0.185, 0.370, 0.925, 1.85 and 3.70) kBq/ml, for the proliferation and colony formation assays, respectively.","PeriodicalId":14261,"journal":{"name":"International Journal of Radiation Biology","volume":"99 8","pages":"1304-1305"},"PeriodicalIF":2.6,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9862628","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"X-radiation of <i>Lotus corniculatus</i> L. seeds improves germination and initial seedling growth.","authors":"Ramazan Beyaz,&nbsp;Jennifer W MacAdam","doi":"10.1080/09553002.2023.2204961","DOIUrl":"10.1080/09553002.2023.2204961","url":null,"abstract":"<p><strong>Purpose: </strong><i>Lotus corniculatus</i> L. (bird's foot trefoil, BFT) is a valuable perennial legume forage species due to its high nutritive value, persistence under grazing, and condensed tannin content that improves ruminant production and prevents bloating. However, it is less preferred by farmers compared with other perennial forage legumes such as alfalfa because of slow germination, slow establishment and low seedling vigor. This study was conducted to determine whether X-ray seed priming could improve these deficiencies.</p><p><strong>Materials and methods: </strong>Seeds of <i>L. corniculatus</i> cv. 'AC Langille' were irradiated at 0, 100, and 300 Gy. Non-irradiated and irradiated seeds were sown on Murashige and Skoog/Gamborg medium under in vitro conditions and cultured for 21 days. Germination percentage, mean germination time (MGT), germination rate index, length of shoot and root, fresh and dry weight of shoot and root, dry matter ratios of shoot and root, water content of shoot and root, and seedling vigor index were measured.</p><p><strong>Results: </strong>The results of this study demonstrated that X-ray seed priming significantly increased the germination percentage of <i>L. corniculatus</i>, increased the germination rate and thereby shortened the MGT, and improved seedling growth. However, X-ray pretreatment also decreased seedling shoot and root biomass.</p><p><strong>Conclusions: </strong>In this study, it is reported for the first time that X-ray seed pretreatment has the potential to address important seedling establishment issues in <i>L. corniculatus</i>.</p>","PeriodicalId":14261,"journal":{"name":"International Journal of Radiation Biology","volume":" ","pages":"1794-1799"},"PeriodicalIF":2.6,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9751800","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effect of electron beam irradiation on the pine wood nematode, <i>Bursaphelenchus xylophilus</i>.","authors":"Junheon Kim, Sujin Lee, Hae-Jun Park","doi":"10.1080/09553002.2023.2232035","DOIUrl":"10.1080/09553002.2023.2232035","url":null,"abstract":"<p><strong>Purpose: </strong>Reproduction inhibition of the pine wood nematode (PWN) by electron beam (e-beam) irradiation both in vitro and in vivo was tested to determine if ionizing radiation could control the PWN by reducing survival and preventing reproduction, thus reducing the risk of pine wilt disease (PWD) spread.</p><p><strong>Materials and methods: </strong>E-beam (10 MeV) irradiation treatment at different doses (0-4 kGy) was applied to PWNs in a Petri dish. Treatment of pine wood logs infested with PWNs was performed at 10 kGy. Mortality was determined by comparing the survival rates before and after irradiation treatment. DNA damage by e-beam irradiation (0-10 kGy) in the PWN was determined using the comet assay.</p><p><strong>Results: </strong>E-beam irradiation increased mortality and suppressed reproduction with increasing doses. The lethal dose (LD) values (kGy) were estimated as follows: LD₅₀ = 2.32, LD₉₀ = 5.03, and LD₉₉ = 9.48. E-beam irradiation of pine wood logs significantly suppressed PWN reproduction. Comets of e-beam-irradiated cells showed an increased tail DNA level and moment with an increasing dose.</p><p><strong>Conclusion: </strong>This study suggests that e-beam irradiation could be used as an alternative method for the management of pine wood logs infested with PWNs.</p>","PeriodicalId":14261,"journal":{"name":"International Journal of Radiation Biology","volume":" ","pages":"1990-1996"},"PeriodicalIF":2.6,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9760389","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"IPW-5371 mitigates the delayed effects of acute radiation exposure in WAG/RijCmcr rats when started 15 days after PBI with bone marrow sparing.","authors":"Brian L Fish, Barry Hart, Tracy Gasperetti, Jayashree Narayanan, Feng Gao, Dana Veley, Lauren Pierce, Heather A Himburg, Thomas MacVittie, Meetha Medhora","doi":"10.1080/09553002.2023.2173825","DOIUrl":"10.1080/09553002.2023.2173825","url":null,"abstract":"<p><strong>Purpose: </strong>To test IPW-5371 for the mitigation of the delayed effects of acute radiation exposure (DEARE). Survivors of acute radiation exposure are at risk for developing delayed multi-organ toxicities; however, there are no FDA-approved medical countermeasures (MCM) to mitigate DEARE.</p><p><strong>Methods: </strong>WAG/RijCmcr female rat model of partial-body irradiation (PBI), by shielding part of one hind leg, was used to test IPW-5371 (7 and 20 mg kg^-1d^-1) for mitigation of lung and kidney DEARE when started 15 d after PBI. Rats were fed known amounts of IPW-5371 using a syringe, instead of delivery by daily oral gavage, sparing exacerbation of esophageal injury by radiation. The primary endpoint, all-cause morbidity was assessed over 215 d. Secondary endpoints: body weight, breathing rate and blood urea nitrogen were also assessed.</p><p><strong>Results: </strong>IPW-5371 enhanced survival (primary endpoint) as well as attenuated secondary endpoints of lung and kidney injuries by radiation.</p><p><strong>Conclusion: </strong>To provide a window for dosimetry and triage, as well as avoid oral delivery during the acute radiation syndrome (ARS), the drug regimen was started at 15 d after 13.5 Gy PBI. The experimental design to test mitigation of DEARE was customized for translation in humans, using an animal model of radiation that was designed to simulate a radiologic attack or accident. The results support advanced development of IPW-5371 to mitigate lethal lung and kidney injuries after irradiation of multiple organs.</p>","PeriodicalId":14261,"journal":{"name":"International Journal of Radiation Biology","volume":"99 7","pages":"1119-1129"},"PeriodicalIF":2.6,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10330589/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9768715","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A simplified protocol for gene expression-based biological dosimetry using peripheral whole blood.","authors":"Shuchi Bhagi,&nbsp;Sudhir Chandna","doi":"10.1080/09553002.2023.2231531","DOIUrl":"10.1080/09553002.2023.2231531","url":null,"abstract":"<p><strong>Purpose: </strong>Assessing alterations in the expression of radiation-responsive genes in peripheral blood cells is considered a promising approach for high-throughput radiation biodosimetry. However, optimization of conditions for storage and transport of blood samples would be critical for obtaining reliable results. Recent studies involved the incubation of isolated peripheral blood mononuclear cells (in cell culture medium) and/or use of RNA stabilizing agents for sample storage, immediately after the <i>ex vivo</i> irradiation of whole blood. We used a simpler protocol by incubating undiluted peripheral whole blood without any RNA stabilizing agent, and studied the impact of storage temperature and incubation time on the expression levels of 19 known radiation responsive genes.</p><p><strong>Materials & methods: </strong>Peripheral whole blood was γ-irradiated <i>ex vivo</i> at room temperature at low (0.5 Gy), moderate (1 Gy, 2 Gy) and high (4 Gy) doses and immediately incubated at two different temperatures at 4 °C or 37 °C for 2h, 4h and 24 h. Using qRT-PCR, mRNA expression levels of <i>CDKN1A, DDB2, GADD45A, FDXR, BAX, BBC3, MYC, PCNA, XPC, ZMAT3, AEN, TRIAP1, CCNG1, RPS27L, CD70, EI24, C12orf5, TNFRSF10B, ASCC3</i> were analyzed at respective time-points and compared with the sham-irradiated controls.</p><p><strong>Results: </strong>Transcriptional responses of all 19 genes did not alter significantly upon incubation of whole blood samples at 4 °C, as compared to untreated controls. However, incubation at 37 °C for 24 h resulted in significant radiation-induced overexpression in 14 out of the 19 genes analyzed (except <i>CDKN1A, BBC3, MYC, CD 70</i> and <i>EI24</i>). Detailed patterns during incubation at 37 °C revealed time-dependent up-regulation of these genes, with <i>DDB2</i> and <i>FDXR</i> showing significant up-regulation both at 4 and 24 h with the highest fold-change observed.</p><p><strong>Conclusion: </strong>Overall, the undiluted whole blood incubated at 37 °C for 24 h was found to elicit most optimal transcriptional response in the genes studied, with most profound overexpression of <i>DDB2</i> and <i>FDXR</i>. We propose that sample storage/transport/post-transit incubation at the physiological temperature for up to 24 h may enhance the sensitivity of gene expression based biodosimetry and facilitate its usage for triage application.</p>","PeriodicalId":14261,"journal":{"name":"International Journal of Radiation Biology","volume":" ","pages":"1692-1701"},"PeriodicalIF":2.6,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9773708","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"An in-vivo study of the combined therapeutic effects of pulsed non-thermal focused ultrasound and radiation for prostate cancer.","authors":"Xiaoming Chen,&nbsp;Dusica Cvetkovic,&nbsp;Lili Chen,&nbsp;C-M Ma","doi":"10.1080/09553002.2023.2214204","DOIUrl":"10.1080/09553002.2023.2214204","url":null,"abstract":"<p><strong>Purpose: </strong>The purpose of this study was to investigate the <i>in vivo</i> combined effects of pulsed focused ultrasound (pFUS) and radiation (RT) for prostate cancer treatment.</p><p><strong>Materials and methods: </strong>An animal prostate tumor model was developed by implanting human LNCaP tumor cells in the prostates of nude mice. Tumor-bearing mice were treated with pFUS, RT or both (pFUS + RT) and compared with a control group. Non-thermal pFUS treatment was delivered by keeping the body temperature below 42 °C as measured real-time by MR thermometry and using a pFUS protocol (1 MHz, 25 W focused ultrasound; 1 Hz pulse rate with a 10% duty cycle for 60 sec for each sonication). Each tumor was covered entirely using 4-8 sonication spots. RT treatment with a dose of 2 Gy was delivered using an external beam (6 MV photon energy with dose rate 300MU/min). Following the treatment, mice were scanned weekly with MRI for tumor volume measurement.</p><p><strong>Results: </strong>The results showed that the tumor volume in the control group increased exponentially to 142 ± 6%, 205 ± 12%, 286 ± 22% and 410 ± 33% at 1, 2, 3 and 4 weeks after treatment, respectively. In contrast, the pFUS group was 29% (<i>p</i> < 0.05), 24% (<i>p</i> < 0.05), 8% and 9% smaller, the RT group was 7%, 10%, 12% and 18% smaller, and the pFUS + RT group was 32%, 39%, 41% and 44% (all with <i>p</i> < 0.05) smaller than the control group at 1, 2, 3, and 4 weeks post treatment, respectively. Tumors treated by pFUS showed an early response (i.e. the first 2 weeks), while the RT group showed a late response. The combined pFUS + RT treatment showed consistent response throughout the post-treatment weeks.</p><p><strong>Conclusions: </strong>These results suggest that RT combined with non-thermal pFUS can significantly delay the tumor growth. The mechanism of tumor cell killing between pFUS and RT may be different. Pulsed FUS shows early tumor growth delay, while RT contributes to the late effect on tumor growth delay. The addition of pFUS to RT significantly enhanced the therapeutic effect for prostate cancer treatment.</p>","PeriodicalId":14261,"journal":{"name":"International Journal of Radiation Biology","volume":" ","pages":"1716-1723"},"PeriodicalIF":2.6,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9840069","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Correction.","authors":"","doi":"10.1080/09553002.2022.2147349","DOIUrl":"10.1080/09553002.2022.2147349","url":null,"abstract":"Figure 1. Experimental design of experiment. Eggs were collected in ovipositing mediums placed inside the main breeding colony and reared until hatch. 14 days post hatch crickets were randomly assigned one of three groups, sham control, irradiated parents and offspring (IPO), or Irradiated Parents and Non-Irradiated Offspring (IPNIO). All three F0 groups were kept separately for the duration of the experiment. At 14 days post hatch, all three groups were transported to the Taylor Source, but only IPO and IPNIO were exposed to radiation (13.92 Gy). F0 Maturation data and F1 eggs were then collected from the three F0 groups. 14 days after the F1 groups hatched, all three groups were transported to the Taylor Source, but only IPO was exposed to radiation (13.92 Gy). Maturation data was collected from the F1 groups.","PeriodicalId":14261,"journal":{"name":"International Journal of Radiation Biology","volume":"99 8","pages":"1303"},"PeriodicalIF":2.6,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9853472","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Protective effects of L-carnitine on X irradiation-induced uterus injury via antioxidant and anti-inflammatory pathways.","authors":"Serkan Karacetin,&nbsp;Meryem Akpolat,&nbsp;Zehra Safi Oz,&nbsp;Ayse Ceylan Hamamcioglu","doi":"10.1080/09553002.2023.2158247","DOIUrl":"https://doi.org/10.1080/09553002.2023.2158247","url":null,"abstract":"Abstract Purpose Ionizing radiation causes oxidative stress induced tissue damage as well as a decline in reproduction incidence. The purpose of our study was to evaluate the effects of L-carnitine on radiation-induced uterine injury. Materials and methods Thirty Wistar albino rats were classified into five groups. Physiological saline was administered intraperitoneally to the control group. A single dose of 8.3 Gy whole body X-irradiation was applied to the radiation-1 and radiation-2 groups. These groups were sacrificed on the 6th hour and 4th day, respectively, after irradiation. Radiation-1 + L-carnitine and radiation-2 + L-carnitine groups received a daily dose of 200 mg/kg L-carnitine in addition to the same dose of irradiation. L-carnitine was also applied one day before and four days after irradiation. Results L-carnitine therapy partially blocks the depletion of the deep glands and radiation-induced flattening of the glandular epithelium and endometrial surface. Proinflammatory cytokines such as IL-1β, IL-6 and TNF-α were found to be significantly expressed in the uterus tissue of irradiated mice. In the radiation groups, NFκB and PARP-1 expressions in uterine tissue was significantly increased compared to L-carnitine treated and the control groups. It was observed that the oxidative stress index increased in the radiation groups, but decreased in the L-carnitine applied groups. Conclusions Our findings showed that L-carnitine has a positive effect on radiation-induced uterine damage. L-carnitine may be a potential safe radio protective agent during radiotherapy for pelvic cancer provided the tumor is not protected from radiation damage to the same extent as the normal tissue is. However, prospective clinical trial studies are necessary to understand its efficacy.","PeriodicalId":14261,"journal":{"name":"International Journal of Radiation Biology","volume":"99 8","pages":"1217-1227"},"PeriodicalIF":2.6,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9863006","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}